Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,487,809	C→T	L1146L (CTG→TTG)	evgS →	sensor histidine kinase EvgS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,487,809	0	C	T	100.0%	64.1 / NA	19	L1146L (CTG→TTG)	evgS	sensor histidine kinase EvgS
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/10);  total (9/10)

ACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGATATCGAAGCCCTGAAAAATAATACGGCGAACGATCTACAACTGATGCAGGAGATTCTCATGACTTTCCAGCATGAAACGCATAAAGATCTACCCGCTGCGTTTCAAGCACTAGAAGCTGGCGATAACAGAACTTTCCATCAGTGTATTCATCGCATCCACGGTGCGGCTAACATCCTGAATTTGCAAAAGTTGATTAATATTAGCCATCAGTTAGAAATAACACCTGTTTCAGATGACAGTAAGCCTGAAATTCTTCAGTTGCTGAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACT  >  NC_000913/2487592‑2488036                                                                                                                                                                                                                          |                                                                                                                                                                                                                                    acacCAAGTTGCTCATATTGCACCTCAGTATCGCCTCCTTCATATCGAAACCCGGAAAAATAATACGGCGAACGATCTACAACTGATGCAGGAGATTCTCATGACTTTCCAGCATGAAACGCATAAAGATCTACCTGCTGCGTTTCAAGCACTAGAAGCTGGCGATAACAGAACTTTCCATCAGTGTATTCATCGCAGCCACGGTGCGGCTAACATCTTGAATTTGCAAAAGTTGATTAATATTAGCCAt                                                                                                                                                                                                     <  1:29136/250‑1 (MQ=255)    ccAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGATATCGAAGCCCTGAAAAATAATACGGCGAACGATCTACAACTGATGCAGGAGATTCTCATGACTTTCCAGCATGAAACGCATAAAGATCTACCCGCTGCGTTTCAAGCACTAGAAGCTGGCGATAACAGAACTTTCCATCAGTGTATTCATCGCATCCACGGTGCGGCTAACATCTTGAATTTGCAAAAGTTGATTAATATTAGCCATCAGt                                                                                                                                                                                                 <  1:216009/251‑1 (MQ=255)          tGCGCATATTGCACCTCAGTATCGCCACCTTGATATCGAAGCCCTGAAAAATAATACGGCGAACGATCTACAACTGATGCAGGAGATTCTCATGACTTTCCAGCATGAAACGCATAAAGATCTACCCGCTGCGTTTCAAGCACTAGAAGCTGGCGATAACAGAACTTTCCATCAGTGTATTCATCGCATCCACGGTGCGGCTAACATCTTGAATTTGCAAAAGTTGATTAATATTAGCCATCAGTTAGaa                                                                                                                                                                                            >  1:155970/1‑250 (MQ=255)           gcgcATATTGCACCTCAGTATCGCCACCTTGATATCGAAGCCCTGAAAAATAATACGGCGAACGATCTACAACTGATTCAGGAGATTCTCATGACTTTCCAGCATGAAACGCATAAAGATCTACCCGCTGCGTTTCAAGCACTAGAAGCTGGCGATAACAGAACTTTCCATCAGTGTATTCATCGCATCCACGGTGCGGCTAACATCTTGAATTTGCAAAAGTTGATTAATAGTAGCCATCAGTTAGAAAt                                                                                                                                                                                          <  1:83123/251‑1 (MQ=255)                           aGTATCGCCACCTTGATATCGAAGCCCTGAAAAATAATACGGCGAACGATCTACAACTGATGCAGGAGATTCTCATGACTTTCCAGCATGAAACGCATAAAGATCTACCCGCTGCGTTTCAAGCACTAGAAGCTGGCGATAACAGAACTTTCCATCAGTGTATTCATCGCATCCACGGTGCGGCTAACATCTTGAATTTGCAAAAGTTGATTAATATTAGCCATCAGTTAGAAATAACACCt                                                                                                                                                                                   <  1:136202/242‑1 (MQ=255)                           aGTATCGCCACCTTGATATCGAAGCCCTGAAAAATAATACGGCGAACGATCTACAACTGATGCAGGAGATTCTCATGACTTTCCAGCATGAAACGCATAAAGATCTACCCGCTGCGTTTCAAGCACTAGAAGCTGGCGATAACAGAACTTTCCATCAGTGTATTCATCGCATCCACGGTGCGGCTAACATCTTGAATTTGCAAAAGTTGATTAATATTAGCCATCAGTTAGAAATAACACCt                                                                                                                                                                                   >  2:136202/1‑242 (MQ=255)                                             tCGAAGCCCTGAAAAATAATACGGCGAACGATCTACAACTGATGCAGGAGATTCTCATGACTTTCCAGCATGAAACGCATAAAGATCTACCCGCTGCGTTTCAAGCACTAGAAGCTGGCGATAACAGAACTTTCCATCAGTGTATTCATCGCATCCACGGTGCGGCTAACATCTTGAATTTGCAAAAGTTGATTAATATTAGCCATCAGTTAGAAATAACACCTGTTTCAGATGACAGTAAGCCTGAAAtt                                                                                                                                                        >  1:129476/1‑251 (MQ=255)                                                aaGCCCTGAAAAATAATACGGCGAACGATCTACAACTGATGCAGGAGATTCTCATGACTTTCCAGCATGAAACGCATAAAGATCTACCCGCTGCGTTTCAAGCACTAGAAGCTGGCGATAACAGAACTTTCCATCAGTGTATTCATCGCATCCACTGTGCGGCTAACATCTTGAATTTGCAAAAGTTGATTAATATTATCCATCAGTTAGAAATAACACCTGTTGCAGATGACAGTAAGCTTGATAttctt                                                                                                                                                     <  2:272167/251‑1 (MQ=255)                                                                                 aaCTGATGCAGGAGACTCTCATGACTTTCCAGCATGAAACGCATAAAGATCTACCCGCTGCGGTTCAAGCACTAGAAGATGGCGATAACAGAACTTTCCATCAGTGTATTCATCGCATCCACGGTGCGGATCACATCTTGAATTTGCAAAAGTTGATTAATATTAGCCATCAGTTAGAAATAACACCTGTTTCAGATGACAGTAAGCCTGAAATTCTTCAGTTGCTGAACTCTGTAAAAGAACACATTGc                                                                                                                     >  2:179003/1‑250 (MQ=255)                                                                                              gaTTCTCATGACTTTCCAGCATGAAACGCATAAAGATCTACCCGCTGCGTTTCAAGCACTAGAAGCTGGCGATAACAGAACTTTCCATCAGTGTATTCATCGCATCCACGGTGCGGCTAACATCTTGAATTTGCAAAAGTTGATTAATATTAGCCATCAGTTAGAAATAACACCTGt                                                                                                                                                                                 <  2:86917/177‑1 (MQ=255)                                                                                              gaTTCTCATGACTTTCCAGCATGAAACGCATAAAGATCTACCCGCTGCGTTTCAAGCACTAGAAGCTGGCGATAACAGAACTTTCCATCAGTGTATTCATCGCATCCACGGTGCGGCTAACATCTTGAATTTGCAAAAGTTGATTAATATTAGCCATCAGTTAGAAATAACACCTGt                                                                                                                                                                                 >  1:86917/1‑177 (MQ=255)                                                                                                                                  tCTACCCGCTGCGTTTCAAGCACTAGAAGCTGGCGATAACAGAACTTTCCATCAGTGTATTCATCGCATCCACGGTGCGGCTAACATCTTGAATTTGCAAAAGTTGATTAATATTAGCCATCAGTTAGAAATAACACCTGTTTCAGATGACAGTAAGCCTGAAATTCTTCAGTTGCTGAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAAt                                                                     >  2:192715/1‑249 (MQ=255)                                                                                                                                     aCCCGCTGCGTTACAAGCACTAGAAGCTGGCGATAACAGAACTTTCCATCAGTGTATTCATCGCATCCACGGTGCGGCTAACATCTTGAATTTGCAAAAGTTGATGAATATTAGCCATCAGTTAGAAATAACACCTGTTTCAGATGACAGTAAGCCTGAAATTCTTCAGTTGCTGACCTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCGGTCTTCTGTCAGAAAAATGACTAAATAGCg                                                                 <  2:206884/250‑1 (MQ=255)                                                                                                                                      cccGCTGCGTTTCAAGCACTAGAAGCTGGCGATAACAGAACTTTCCATCAGTGTATTCATCGCATTCACGGTGCGGCTAACATCTTGAATTGGCAAAAGTTGATTAATATTAGCCATCAGTTAGAAATAACACTTGTTTCAGATGACAGTAAGCCTGAAATTCTTCAGTTGCTGAACTCTGTATAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGc                                                               <  1:285611/251‑1 (MQ=255)                                                                                                                                                          aGAAGCTGGCGATAACAGAACTTTCCATCAGTGTATTCATCGCATCCACGGTGCGGCTAACATCTTGAATTTGCAAAAGTTGATTAATATTAGCCATCAGTTAGAAATAACACCTGTTTCAGATGACAGTAAGCCTGAAATTCTTCAGTTGCTGAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAAtgtg                                           >  2:8584/1‑251 (MQ=255)                                                                                                                                                                  gCGATAACAGAACTTTCCATCAGTGTATTCATCGCATCCACGGTGCGGCTAACATCTTGAATTTGCAAAAGTTGATTAATATTAGCCATCAGTTAGAAATAACACCTGTTTCAGATGACAGTAAGCCTGAAATTCTTCAGTTGCTGAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAAAAGCGGCTCCCACAATGTTCAAATGTGGGAGCTAt                                   <  1:218313/251‑1 (MQ=255)                                                                                                                                                                               tttCCATCAGTGTAATCATCGCATCCCCGGTGCGGCTAACATCTTGAATTTGCAAAAGTTGATTAATATTAGCCATCAGATAGAAATAACACCTGTTTCAGATGACAGTAAGCCTGAAATTCTTCAGTTGCTGAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATc                      >  2:171660/1‑251 (MQ=255)                                                                                                                                                                                            aTTCATCGCATCCACGGTGCGGCTAACATCTTGAATTTGCAAAAGTTGATTAATATTAGCCATCAGTTAGAAATAACACCTGTTTCAGATGACAGTAAGCCTGAAATTCTTCAGTTGCTGAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTatgat         >  1:172190/1‑251 (MQ=255)                                                                                                                                                                                                   gCATCCACGGTGCGGCTAACATCTTGAATTTGCAAAAGTTGATTAATATTAGCCATCAGTTAGAAATAACACCTGTTTCAGATGACAGTAAGCCTGAAATTCTTCAGTTGCTGAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTTCATTTATGATGAGAACt  <  1:83330/251‑1 (MQ=255)                                                                                                                                                                                                                          |                                                                                                                                                                                                                                    ACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGATATCGAAGCCCTGAAAAATAATACGGCGAACGATCTACAACTGATGCAGGAGATTCTCATGACTTTCCAGCATGAAACGCATAAAGATCTACCCGCTGCGTTTCAAGCACTAGAAGCTGGCGATAACAGAACTTTCCATCAGTGTATTCATCGCATCCACGGTGCGGCTAACATCCTGAATTTGCAAAAGTTGATTAATATTAGCCATCAGTTAGAAATAACACCTGTTTCAGATGACAGTAAGCCTGAAATTCTTCAGTTGCTGAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACT  >  NC_000913/2487592‑2488036

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑