Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,274,557	A→G	T127A (ACG→GCG)	yejB →	putative oligopeptide ABC transporter membrane subunit YejB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,274,557	0	A	G	100.0%	67.6 / NA	21	T127A (ACG→GCG)	yejB	putative oligopeptide ABC transporter membrane subunit YejB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/8);  total (13/8)

GCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGACGCTGATTAAAGACAGTCTGCCGGTTTCCATCACCCTCGGATTGTGGAGCACGCTGATTATCTATCTGGTGTCGATTCCGTTAGGCATTCGCAAAGCTGTTTATAATGGGAGCCGCTTTGACGTCTGGAGTAGCGCATTTATCATCATCGGCTACGCCATTCCGGCCTTTTTGTTTGCCATCCTGCTGATTGTCTTCTTC  >  NC_000913/2274317‑2274757                                                                                                                                                                                                                                                 |                                                                                                                                                                                                         gCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGGCAGCCATGCGCAAACGGGGGGCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGGCGCTggatt                                                                                                                                                                                                 >  2:159779/1‑246 (MQ=255)   cGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGGCGCTGATTaa                                                                                                                                                                                                >  1:94830/1‑249 (MQ=255)                   gcAGGCGGGGAAGGTGTTCGGGCCAGCCCTGCGCAAACGGGGGTCGGCAATATCAGCGACAGGAAATACCCTGGCGGACGCGGGTTTGATCCCGAAAGGATCGCTGAGAACACTCATCGCTTCGGTTTTTATAAGCCGATACACGAACGTTACTTCAAAATGCTCTGGGGCTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCCGGCCCTCGGGGCTGGCGCCGATTAAAGACAATCTGCCGGtttt                                                                                                                                                                              >  2:268764/1‑250 (MQ=255)                      gacggcgAAGGTGTTCGAGCCAGCCATGCGCAAACGGGGGTCGGCATTATCAGCGACAGTAATTACCGAGGCGGAACAGGATTAGATAAAGAACTGATCGCCGAGATCACTCATCGCTACGGGTTTACTAAGCGGATCCACGAACGTTACTTCAAAATGCTATGGGACTACATACGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGATGCTGGCGCTGTTTAAAGACAGGCTGCCgggttacat                                                                                                                                                                           >  2:269911/7‑244 (MQ=255)                             aaGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGGCGCTGATTAAAGACAGTCTGCCGGTTTCCATCACCCTc                                                                                                                                                                    <  1:268764/251‑1 (MQ=255)                                gtgtTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGGCGCTGATTAAAGACAGTCTGCCGGTTTCCATCACCCTCGGa                                                                                                                                                                 <  1:107772/251‑1 (MQ=255)                                                         gggTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAAATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGGCGCTGATTAAAGACAGTCTGCCGGTTTCCATCACCCTCGGATTGTGGAGCACGCTGATgatctatc                                                                                                                                        <  2:231289/251‑1 (MQ=255)                                                               aggCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGGCGCTGATTAAAGACAGTCTGCCGGTTTCCATCACCCTCGGATTGTGGAGCACGCTGATTATCTATCTGgtgt                                                                                                                                  >  2:51076/2‑251 (MQ=255)                                                                      atCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGGCGCTGATTAAAGACAGTCTGCCGGTTTCCATCACCCTCGGATTGTGGAGCACGCTGATTATCTATCTGGTGTCGATTc                                                                                                                            <  2:94830/250‑1 (MQ=255)                                                                                            gCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGGCGCTGATTAAAGACAGTCTGCCGGTTTCCATCACCCTCGGATTGTGGAGCACGCTGATTATCTATCTGGTGTCGATTCCGTTAGGCATTCGCAAAGCTGtt                                                                                                     >  2:148809/1‑251 (MQ=255)                                                                                                    ggATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGGCGCTGATTAAAGACAGTCTGCCGGTTTCCATCACCCTCGGATTGTGGAGCACGCTGATTATCTATCTGGTGTCGATTCCGTTAGGCATTCGCAAAGCTGTTTATAATgg                                                                                             >  2:96026/1‑251 (MQ=255)                                                                                                                  aGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGGCGCTGATTAAAGACAGTCTGCCGGTTTCCATCACCCTCGGATTGTGGAGCACGCTGATTATCTATCTGGTGTCGATTCCGTTAGGCATTCGCAAAGCTGTTTATAATGGGAGCCGCTTTGACg                                                                               >  2:68358/1‑251 (MQ=255)                                                                                                                             agaTCACTCATCGCTACGGTTTTGATAAGCCGATCCTCGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGGCGCTGATTAAAGACAGTCTGCCGGTTTCCATCACCCTCGGATTGTGGAGCACGCTGATTATCTATCTGGTGGCGATTCCGTTAGGCATTCGCAAAGCTGTTTATAATGGGAGCCGCTTTGACGTCTGGAGTAgc                                                                    <  1:147970/251‑1 (MQ=255)                                                                                                                                                             aTCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGGCGCTGATTAAAGACAGTCTGCCGGTTTCCATCACCCTCGGATTGTGGAGCACGCTGATTATCTATCTGGTGTCGATTCCGTTAGGCATTCGCAAAGCTGTTTATAATGGGAGCCGCTTTGACGTCTGGAGTAGCGCATTTATCATCATCGGCTACGCCATTCCGGc                                    >  1:40122/1‑251 (MQ=255)                                                                                                                                                               ccACGAAAGTTACTTCAAAATGCTCTGGGACTAAATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGGCGCTGATTAAAGACAGTCTGCCGGTTTCCATCACCCTCGGATTGTGGAGCAAGCTGATTATCTATCTGGTGTCGATTCCGTTAGGCATTCGCAAAGCTGTTTATACTGGGAGCCGCTTTGACGTCTGGAGTAGCGCATTTATCATCATCGGCTACGCCATTCCGGCCt                                  >  1:76752/1‑251 (MQ=255)                                                                                                                                                                        gtacgtCAAAATGATCTTGGTGTACATCCGGTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGGCGCTGATTAAAGACAGTCTGCCGGTTTTCATCTCCCTCGGATTGTGGAGCACGCTGATTATCTATCTGGTGTCGATTCCGGTAGGCATTCGCAAAGCTGTTTATAATGGGAGCCGCGTTGACGTCTGGATTAGCGCATTTAGCATCATCGGCTACGCCATTCCGGCCTTtttgtttg                         <  2:201943/246‑1 (MQ=255)                                                                                                                                                                              cAAACTGCTCTGGGACTACATACGCTTTGATTTTGCCGACAGCCTCATTCGCAGAGCCTCGGTGCTGGCGCTGATTACAGACAGTCTGCCGGCTTCCATCACACTCGGATTGTGGAGCACGCTGACTATCTATATGGTCACGATTCCGTTAGGCATTCGCAAAGCTGTTTCTATTGGTAGCCGCTTTGTCGTCTGGAGTAGCGCATTTATCATCATCGGCTACGCGATTCCGGACTTTTTGTTTGCCNTcc                   >  2:257087/1‑251 (MQ=255)                                                                                                                                                                                           gACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGGCGCTGATTAAAGACAGTCTGCCGGTTTCCATCACCCTCGGATTGTGGAGCACGCTGATTATCTATCTGGTGTCGATTCCGTTAGGCATTCGCAAAGCTGTTTATAATGGGAGCCGCTTTGACGTCTGGAGTAGCGCATTTATCATCATCGGCTACGCCATTCCGGCCTTTTTGTTTGCCATCCTGCTGATTGtctt      >  2:230511/1‑251 (MQ=255)                                                                                                                                                                                               aCATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGGCGCTGATTAAAGACAGTCTGCCGGTTTCCATCACCCTCGGATTGTGGAGCACGCTGATTATCTATCTGGTGTCGATTCCGTTAGGCATTCGCAAAGCTGTTTATAATGGGAGCCGCTTTGACGTCTGGAGTAGCGCATTTATCATCATCGGCTACGCCATTCCGGCCTTTTTGTTTGCCATCCTGCTGATTGTCttcttc  <  1:159779/251‑1 (MQ=255)                                                                                                                                                                                                cATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGGCGCTGATTAAAGACAGTCTGCCGGTTTCCATCACCCTCGGATTGTGGAGCACGCTGATTATCTATCTGGTGTCGATTCCGTTAGGCATTCGCAAAGCTGTTTATAATgg                                                                                             >  1:133345/1‑159 (MQ=255)                                                                                                                                                                                                cATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGGCGCTGATTAAAGACAGTCTGCCGGTTTCCATCACCCTCGGATTGTGGAGCACGCTGATTATCTATCTGGTGTCGATTCCGTTAGGCATTCGCAAAGCTGTGTATAATgg                                                                                             <  2:133345/159‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                         GCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGACGCTGATTAAAGACAGTCTGCCGGTTTCCATCACCCTCGGATTGTGGAGCACGCTGATTATCTATCTGGTGTCGATTCCGTTAGGCATTCGCAAAGCTGTTTATAATGGGAGCCGCTTTGACGTCTGGAGTAGCGCATTTATCATCATCGGCTACGCCATTCCGGCCTTTTTGTTTGCCATCCTGCTGATTGTCTTCTTC  >  NC_000913/2274317‑2274757

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑