Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 551,779 C→T P272P (CCG→CCA)  purK ← 5‑(carboxyamino)imidazole ribonucleotide synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913551,7790CT97.9% 156.6 / ‑6.7 50P272P (CCG→CCA) purK5‑(carboxyamino)imidazole ribonucleotide synthase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (25/22);  total (27/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

TCTGCGCCCAAATCACGCCGCTGGCATATTCCGGCGGCAGCAGCGGGATTAAGGCTTCCAGCGTCGCAGTCAGACGCGATGTGTCGCTGTCGGTCAAATTCAGATGCCCCACTTTACGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCACCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGATTATTCACCACTGGTTGCGGTAACGGCAGATCGGTAATCGCCCGCAGATGCAGCTCAAACTGGCTGATGCTGGCACCGTTTTGTGTCCAGTGACCGCTGTTATGCACACGCGGTGCCAGTTCGTTGATCAACAGACCTTGCGGGGTGACAAAACACTCCATCGCCATCACGCCCACATAGCCCAGCTCCTGCATAATCGCCGACAGCATCTCTTCGGCTTGCGCCTGCTGCTGTGCGTTGGCCTGCGGAAAA  >  NC_000913/551541‑552013
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                          
tctGCGCCCAAATCACGCCGCTGGCATATTCCGGCGGCAGCAGCGGGATTAAGGCTTCCAGCGTCGCAGTCAGACGCGATGTGTCGCTGTCGGTCAAATTCAGATGCCCCACTTTACGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCACCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGATTATTCACCACTGGTTGTGGTAACGGc                                                                                                                                                                                                                                   >  2:66379/1‑248 (MQ=255)
 ctGCGCCCAAATCACGCCGCTGGCATATTCCGGCGGCAGCAGCGGGATTAAGGCTTCCAGCGTCGCAGTCAGACGCGATGTGTCGCTGTCGGTCAAATTCAGATGCCCCACTTTACGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCACCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTCCCAATCAGATTGATCATCACCGACGGATTATTCACCACTGGTTGTGGTAACGGc                                                                                                                                                                                                                                   >  2:14665/1‑247 (MQ=255)
   gcgcCCAAATCACGCCGCTGGCATATTCCGGCGGCAGCAGCGGGATTAAGGCTTCCAGCGTCGCAGTCAGACGCGATGTGTCGCTGTCGGTCAAATTCAGATGCCCCACTTTACGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCACCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGATTATTCACCACTGGTTGTGGTAACGGCAGATc                                                                                                                                                                                                                              <  1:11704/250‑1 (MQ=255)
     gcCCAAATCACGCCGCTGGCATATTCCGGCGGCAGCAGCGGGATTAAGGCTTCCAGCGTCGCAGTCAGACGCGATGTGTCGCTGTCGGTCAAATTCAGATGCCCCACTTTACGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCACCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGATTATTCACCACTGGTTGTGGTAACGGCAGATCGGt                                                                                                                                                                                                                           <  2:88785/251‑1 (MQ=255)
      cgcAAATCACGCCGATGGCATAAGCCTGAGGCAGCAGCGGTATTAAGGCTTCCCGCGACGCAGTCAGACGCGATGTGGCGCTGTCGGTCAACGTCAGATGCCCCACTTTACGCCCCGGACGGACGTCTTTGTCGTACCAGTGCATATGCACCATCGGCATTTTCAGCCAGTCATATTTCACATCGCTGCCAATCAGATTGATCATCTCCGACGGATTATTCACCACTGGTTTTGGTAACGGCAGATCGGtt                                                                                                                                                                                                                          <  2:31058/249‑2 (MQ=255)
       ccAAATCACGCCGCTGGCATATTCCGGCGGCAGCAGCGGGATTAAGGCTTCCAGCGTCGCAGTCAGACGCGATGTGTCGCTGTCGGTCAAATTCAGATGCCCCACTTTACGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCACCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGATTATTCACCACTGGTTGTGGTAACGGCAGATCGGTa                                                                                                                                                                                                                          >  1:127706/1‑250 (MQ=255)
        cAAATCACGCCGCTGGCATATTCCGGCGGCAGCAGCGGGATTAAGGCTTCCAGCGTCGCAGTCAGACGCGATGTGTCGCTGTCGGTCAAATTCAGATGCCCCACTTTACGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCACCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGATTATTCACCACTGGTTGTGGTAACGGCAGATCGGTAAt                                                                                                                                                                                                                        >  2:205971/1‑251 (MQ=255)
            tCACGCCGCTGGCATATTCCGGCGGCAGCAGCGGGATTAAGGCTTCCAGCGTCGCAGTCAGACGCGATGTGTCGCTGTCGGTCAAATTCAGATGCCCCACTTTACGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCACCAGCGGCAGTTTCAGCCAGTCATACTTCACATCGCTACCAATCAGATTGATCATCACCGACGGATTATTCACCACTGGTTGTGGTAACGGCAGATCGGTAATCGcc                                                                                                                                                                                                                    >  1:133745/1‑251 (MQ=255)
                                ggcggcAGCAGCGGGATTAAGGCTTCCAGCGTCGCAGTCAGACGCGATGTGTCGCTGTCGGTCAAATTCAGATGCCCCACTTTACGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCACCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGATTATTCACCACTGGTTGTGGTAACGGCAGATCGGTAATCGCCCGCAGATGCAGCTCAAACTg                                                                                                                                                                                                >  1:219320/1‑251 (MQ=255)
                                             ggATTAAGGCTTCCAGCGTCGCAGTCAGACGCGATGTGTCGCTGACGGTCAAATTCATAGGCACCACTTTACGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCACCAGCGGCAGTTTCAGCCAGGCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGATTATTCACCACTGGTTGGGGTAACAGCAGATCGGTAATCGCCCGCAGATGCAGCTCAAACTGGCTGATGCTGGCa                                                                                                                                                                                   <  1:138998/251‑1 (MQ=255)
                                                 tAAGGCTTCCAGCGTCGCAGTCAGACGCGATGTGTCGCTGTCGGTCAAATTCAGATGCCCCACTTTACGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCACCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGATTATTCACCACTGGTTGTGGTAACGGCAGATCGGTAATCGCCCGCAGATGCAGCTCAAACTGGCTGATGCTGGCACCGt                                                                                                                                                                               >  2:198524/1‑251 (MQ=255)
                                                          cAGCGTCGCAGTCAGACGCGATGTGTCGCTGTCGGTCAAATTCAGATGCCCCACTTTACGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCACCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGATTATTCACCACTGGTTGTGGTAACGGCAGATCGGTAATCGCCCGCAGATGCAGCTCAAACTGGCTGATGCTGGCACCGTTTTGTGTcc                                                                                                                                                                      <  1:87578/251‑1 (MQ=255)
                                                                                  gtCGCTGTCGGTCAAATTCAGATGCCCCACTTTACGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCACCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGATTATTCACCACTGGTTGTGGTAACGGCAGATCGGTAATCGCCCGCAGATGCAGCTCAAACTGGCTGATGCTGGCACCGTTTTGTGTCCAGTGACCGCTGTTATGCACAcgcg                                                                                                                                              >  1:270922/1‑251 (MQ=255)
                                                                                    cGCTGTCGGTCAAATTCAGATGCCCCACTTTACGCCCCGGACGGCCTTCTTTGTCGTACCAGTGCAGATGCACCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGATTATTCACCACTGGTTGTGGTAACGGCAGATCGGTAATCGCCCGCAGATGCAGCTCAAACTGGCTGATGCTGGCACCGTTTTGTGTCCAGTGACCGCTGTTATGCACACGCgg                                                                                                                                             <  2:33227/250‑1 (MQ=255)
                                                                                                  ttCAGATGCCCCACTTTACGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCACCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGATTATTCACCACTGGTTGTGGTAACGGCAGATCGGTAATCGCCCGCAGATGCAGCTCAAACTGGCTGATGCTGGCACCGTTTTGTGTCCAGTGACCGCTGTTATGCACACGCGGTGCCAGTTCGTTg                                                                                                                                <  2:63329/249‑1 (MQ=255)
                                                                                                  ttCAGATGCCCCACTTTACGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCACCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGATTATTCACCACTGGTTGTGGTAACGGCAGATCGGTAATCGCCCGCAGATGCAGCTCAAACTGGCTGATGCTGGCACCGTTTTGTGTCCAGTGACCGCTGTTATGCACACGCGGTGCCAGTTCGTTg                                                                                                                                >  1:63329/1‑249 (MQ=255)
                                                                                                   tCAGATGCCCCACTTTACGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCACCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGATTATTCACCACTGGTTGTGGTAACGGCAGATCGGTAATCGCCCGCAGATGCAGCTCAAACTGGCTGATGCTGGCACCGTTTTGTGTCCAGTGACCGCTGTTATGCACACGCGGTGCCAGTTCGTTGAt                                                                                                                              <  1:66379/250‑1 (MQ=255)
                                                                                                    cAGATGCCCCACTTTACGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCACCAGCGGCAGTTTAAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGATTATTCACCACTGGTTGAGGTAACGGCAGATCGGTAATCGCCCGCAGATGCAGCTCAAACTGGCTGATGCTGGCACCGTTTTGTGTCCAGTGACCGCTGTTATGAACACGCGGTGCCAGTTCGTTGATc                                                                                                                             >  1:193828/1‑250 (MQ=255)
                                                                                                      gATGCCCCACTTTACGCCCCGGACGGACTTCTTTGTCGTACCAGTGAAGATGCACCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGATTATTCACCACTGGTTGTGGTAACGGCAGATCGGTAATCGCCCGCAGATGAAGCTCACACTGGCTGATGCTGGCACCGTTTTGTGTCCAGTGACCGCTGTTATGCACACGCGGGGCCAGTTCGTTGATCAAc                                                                                                                          >  1:128470/1‑251 (MQ=255)
                                                                                                             cACTTTACGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCACCAGCGGCAGTTTCAGCCAGTCATAATTAACATCGCTACCAATCAGATTGATCATCACCGACGGATTATTCACCACTGGTTGTGGTAACGGCAGATCGGTAATCGCCCGCAGATGCAGCTCAAACTGGCTGATGCTGGCACCGTTTTGTGTCCAGTGACCGCTGTTACGCACCCGCGGTGCCAGTTCGTTGATCAACCGACCtt                                                                                                                   >  2:166764/1‑251 (MQ=255)
                                                                                                              aCTTTACGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCACCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGATTATTCACCACTGGTTGTGGTAACGGCAGATCGGTAATCGCCCGCAGATGCAGCTCAAACTGGCTGATGCTGGCACCGTTTTGTGTCCAGTGACCGCTGTTATGCACCCGCGGTGCCCGTTCGTTGATCAACAGACCTTg                                                                                                                  >  2:24343/1‑251 (MQ=255)
                                                                                                                             cggacTTCTTTGTCGTACCAGTGCAGATGCACCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGATTATTCACCACTGGTTGTGGTAACGGCAGATCGGTAATCGCCCGCAGATGCAGCTCAAACTGGCTGATGCTGGCACCGTTTTGTGTCCAGTGACCGCTGTTATGCACACGCGGTGCCAGTTCGTTGATCAACAGACCTTGCGGGGTGACAAAac                                                                                                    >  1:47459/1‑250 (MQ=255)
                                                                                                                                  ttcttTGTCGTACCAGTGCAGATGCACCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCCATCAGATTGATCATCACCGACGGATTATTCACCACTGGTTGTGGTAACGGCAGATCGGTAATCGCCCGCAGATGCAGCTCAAACTGGCTGATGCTGGCACCGTTTTGTGTCCAGTGACCGCTGTTATGCACACGCGGTGCCAGTTAGTTGATCAACACACATTGCGGGTTGACAAAACACTcc                                                                                               >  2:233662/1‑250 (MQ=255)
                                                                                                                                      ttGTCGTACCAGTGCAGATGCACCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGATTATTCACCACTGGTTGTGGTAACGGCAGATCGGTAATCGCCCGCAGATGCAGCTCAAACTGGCTGATGCTGGCACCGTTTTGTGTCCAGTGACCGCTGTTATGCACACTCGGTGCCAGTTCGTTGATCAACAGACCTTGCGGGGTGACAAAACACTCCAt                                                                                             <  1:24343/248‑1 (MQ=255)
                                                                                                                                              ccAGTGCAGATGCACCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGATTATTCACCACTGGTTGTGGTAACGGCAGATCGGTAATCGCCCGCAGATGCAGCTCAAACTGGCTGATGCTGGCACCGTTTTGTGTCCAGTGACCGCTGTTATGCACACGCGGTGCCAGTTCGTTGATCAACAGACCTTGCGGGGTGACAAAACACTCCATCGCCATCACGc                                                                                  >  1:121000/1‑251 (MQ=255)
                                                                                                                                                 gTGCAGATGCCCCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGATTATTCACCACTGGTTGTGGTAACGGCAGATCGGTAATCGCCCGCAGATGCAGCTCAAACTGGCTGATGCTGGCACCGTTTTGTGTCCAGTGACCGCTGTTATGCACACGCGGTGCCAGTTCGTTGATCAACAGACCTTGCGGGGTGACAAAACACTCCATCGCCATCACGCCca                                                                               <  1:238951/251‑1 (MQ=255)
                                                                                                                                                 gTGCAGATGCACCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGATTATTCACCACTGGTTGTGGTAACGGCAGATCGGTAATCGCCCGCAGATGCAGCTCAAACTGGCTGATGCTGGCACCGTTTTGTGTCCAGTGACCGCTGTTATGCACACGCGGTGCCAGTTCGTTGATCAACAGACCTTGCGGGGTGACAAAACACTCCATCGCCATCACGcccc                                                                               >  1:202200/1‑250 (MQ=255)
                                                                                                                                                        tgctccAGCGTCAGTTGCAGCAAGTCATAATTCCCAACGCTACCAATCAGATTGTTCATCGCCGACGGATTATTCACCGCTGGGTGTGGTAACTGCAGATCGGTAAGCGCCCGCCTATGCGGCTCAAACTTGCTGATGCTTGCCCCGTTTTGTCTCCTGTGACCTCTGTTATGCCCACGCGGTGCCAGTTCGTCGATCAACAGACCTTGCTTGGTGACAAAACACTCCATCGCCATCACGCCCACATAGc                                                                         <  2:232040/246‑1 (MQ=255)
                                                                                                                                                            ccAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGATTATTCACCACTGGTTGTGGTAACGGCAGATCGGTAATCGCCCGCAGATGCAGCTCAAACTGGCTGATGCTGGCACCGTTTTGTGTCCAGTGACCGCTGTTATGCACACGCGGTGCCAGTTCGTTGATCAACAGACCTTGCGGGGTGACAAAACACTCCATCGCCATCACGCCCACATAGCCCAGc                                                                    >  1:7132/1‑251 (MQ=255)
                                                                                                                                                             cAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGATTATTCACCACTGGTTGTGGTAACGGCAGATCGGTAATCGCCCGCAGATGCAGCTCAAACTGGCTGATGCTGGCACCGTTTTGTGTCCAGTGACCGCTGTTATGCACACGCGGTGCCAGTTCGTTGATCAACAGACCTTGCGGGGTGACAAAACACTCCATCGCCATCACGCCCACATAGCCCAGCt                                                                   <  1:14665/251‑1 (MQ=255)
                                                                                                                                                               gCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGATTATTCACCACTGGTTGTGGTAACGGCAGATCGGTAATCGCCCGCAGATGCAGCTCAAACTGGCTGATGCTGGCACCGTTTTGTGTCCAGTGACCGCTGTTATGCACACGCGGTGCCAGTTCGTTGATCAACAGACCTTGCGGGGTGACAAAACACTCCATCGCCATCACGCCCACATAGCCCAGCTcc                                                                 <  2:104564/251‑1 (MQ=255)
                                                                                                                                                                cGGCAGTTTCAGCCAGTCCTAATTCACATCGCTACCCATCAGATTGATCATCACCTACGGATTATTTACCACTGGTTGTGGTAACGGCAGATCGGTAATCGCCCGCAGATGCAGCTCAAACTGGCTGATGCTGGCACCGTTTTGTGTCCAGTGACCGCTGTTATGCACACGCGGTGCCAGTTCGTTGATCAACAGACCTTGCGGGGTGACAAAACCCTCCATCGCCATCACGCCCATATAGCCCAGCTCCt                                                                <  2:169326/251‑1 (MQ=255)
                                                                                                                                                                   cAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGATTATTCACCACTGGTTGTGGTAACGGCAGATCGGTAATCGCCCGCAGATGCAGCTCAAACTGGCTGATGCTGGCACCGTTTTGTGTCCAGTGACCGCTGTTATGCACACGCGGTGCCAGTTCGTTGATCAACAGACCTTGCGGGGTGACAAAACACTCCATCGCCATCACGCCCACATAGCCCAGCTCCTGCa                                                             <  2:219320/251‑1 (MQ=255)
                                                                                                                                                                    aGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGATTATTCACCACTGGTTGTGGTAACGGCAGATCGGTAATCGCCCGCAGATGCAGCTCAAACTGGCTGATGCTGGCACCGTTTTGTGTCCAGTGACCGCTGTTATGCACACGCGGTGCCAGTTCGTTGATCAACAGACCTTGCGGGGTGACAAAACACTCCATCGCCATCACGCCCACATAGCCCAGCTCCTGCAt                                                            <  1:214987/251‑1 (MQ=255)
                                                                                                                                                                             cAGTCATAATTCACATCGCTACCAAGCAGATTGATCATCACCGACGGATTATTCACCACTGGTTGTGGTAACGGCAGATCGGTAATCGCCCGCAGATGCAGCTCAAACTGGCTGATGCTGGCACCGTTTTGTGTCCAGTGACCGCTGTTATGCACACGCGGTGCCAGTTCGTTGATCAACAGACCTTGCGGGGTGACAAAACACTCCATCGCCATCACGCCCACATAGCCCAGCTCCTGCATAATCGCCGa                                                   <  1:58384/251‑1 (MQ=255)
                                                                                                                                                                               gTCATAATTCACATCGCTACCAATCAGATTGATAATCACCGACGGATTATTCACCACTGGTTGTGGTAACGGCAGATCGGTAATCGCCCGCAGATGCAGCTCAAACTGGCTGATGCTGGCACCGTTTTGTGTCCAGTGACCGCTGTTATGCACACGCGGTGCCAGTTCGTTGATCAACAGACCTTGCGGGGTGACAAAACACTCCATCGCCATAACGCCCACATAGCCCAGCTCCTGCATAATCGCCGACa                                                 <  2:121000/251‑1 (MQ=255)
                                                                                                                                                                                         ccaTCGCTACCAATCAGATTGATCCTCACCGACGGATTATTCACCACTGGTTGTGGTAACGGCAGATCGGTAATCGCCCGCAGATGCAGCTCAAGCTGGCTGATGCTGGCACCGTTTTGTGTCCAGTGACCGCTGTTATGCACACGCGGTGCCAGTTCGTTGATCAACAGACCTTGCGGGGTGACAAAACACTCCATCGCCATCACGCCCACATAGCCCAGCTCCTGCATAGTCGTCGACAGCATCTCTTc                                       <  2:202438/250‑1 (MQ=255)
                                                                                                                                                                                                   cAATCAGATTGATCATCACCGACGGATTATTCACCACTGGTTGTGGTAACGGCAGATCGGTAATCGCCCGCAGATGCAGCTCAAACTGGCTGATGCTGGCACCGTTTTGTGTCCAGTGACCGCTGTTATGCACACGCGGTGCCAGTTCGTTGATCAACAGACCTTGCGGGGTGACAAAACACTCCATCGCCATCACGCCCACATAGCCCa                                                                      <  2:229833/210‑1 (MQ=255)
                                                                                                                                                                                                   cAATCAGATTGATCATCACCGACGGATTATTCACCACTGGTTGTGGTAACGGCAGATCGGTAATCGCCCGCAGATGCAGCTCAAACTGGCTGATGCTGGCACCGTTTTGTGTCCAGTGACCGCTGTTATGCACACGCGGTGCCAGTTCGTTGATCAACAGACCTTGCGGGGTGACAAAACACGCCATCGCCATCACGCCCACATAGCCCAGCTCCTGCATAATCGCCGACAGCATCTCTTCGGCTTGCGcc                             <  2:77706/251‑1 (MQ=255)
                                                                                                                                                                                                   cAATCAGATTGATCATCACCGACGGATTATTCACCACTGGTTGTGGTAACGGCAGATCGGTAATCGCCCGCAGATGCAGCTCAAACTGGCTGATGCTGGCACCGTTTTGTGTCCAGTGACCGCTGTTATGCACACGCGGTGCCAGTTCGTTGATCAACAGAACTTGCGGGGTGACAAAACACTCCATCGCCATCACGCCCACATAGCCCa                                                                      >  1:229833/1‑210 (MQ=255)
                                                                                                                                                                                                             gATCATCACCGACGGATTATTCACCACTGGTTGCGGTAACGGCAGATCGGTAATCGCCCGCAGATGCAGCTCAAACTGGCTGATGCTGGCACCGTTTTGTGTCCAGTGACCGCTGTTATGCACACGCGGTGCCAGTTCGTTGATCAACAGACCTTGCGGGGTGACAAAACTCTCCATCGCCATCACGCCCACATAGCCCAGCTCCCGCACAATCGCCGACAGCATCTCTCCGGCTTGCGCCCGCTGCtgtg                   >  1:15475/1‑251 (MQ=255)
                                                                                                                                                                                                              atcatcACCGACGGATTATTCACCACTGGTTGTGGTAACGGCAGATCGGTAATCGCCCGCAGATGCAGCTCAAACTGGCTGATGCTGGCACCGTTTTGTGTCCAGTTACCGCTGTTATGCACACGCGGTGCCAGTTCGTTGATCAACAGACCTTGCGGGGTGACAAAACACTCCATCGCCATCACGCCCACCTAGCCCAGCTCCTGCATAATCGCCGACAGCATCTCTTCGGCTTTCGCCTGCTGCTNTGc                  >  2:258215/1‑251 (MQ=255)
                                                                                                                                                                                                               tcatcaCCGACGGATTATTCACCACTGGTTGTGGAAACGGCAGATCGGTAATCGCCCGCAGATGCAGCTCAAACTGGCTGATGCTGGCACCGGTTTGTGTCCAGGGACCGCTGTTATGAACAAGCGGTGCCAGTTCGTTGATCAACAGACCTTGCGGGGTGACAAAACACTCCATCGCCATCACGCCCACATAGCCCAGCTCCTGCATAATCGCCGACAGCATATCTTCGGCTTGCGCCTGCTGCTGTGCg                 >  2:244610/1‑251 (MQ=255)
                                                                                                                                                                                                                      cGACGGATTATTCACCACTGGTTGTGGTAACGGCAGATCGGTAATCGCCCGCAGATGCAGCTCAAACTGGCTGATGCTGGCACCGTTTTGTGTCCAGTGACCGATGTTATGCACACGCGGTGCCAGTTCGTTGATCAACAGACCTTGCGGGGTGACAAAACACTCCATCGCCATCACGCCCACATAGCCCAGCTCCTGCATAATCGCCGACAGCATCTCTTCGGCTTGCGCCTGCTGCTGTGCGTTGGCCt          >  1:138639/1‑251 (MQ=255)
                                                                                                                                                                                                                       gACGGATTATTCACCACTGGTTGTGGTAACGGCAGATCGGTAATCGCCCGCAGATGCAGCTCAAACTGGCTGATGCTGGCACCGTTTTGTGTCCAGTGACCGCTGTTATGCACACGCGGTGCCAGTTCGTTGATCAACAGACCTTGCGGGGTGACAAAACACTCCATCGCCATCACGCCCACATAGCCCAGCTCCTGCATAATCGCCGACAGCATCTCTTCGGCTTGCGCCTGCTGCTGTGCGTTGGCCTg         <  1:205971/251‑1 (MQ=255)
                                                                                                                                                                                                                       gACGGATTATTCACCACTGGTTGTGGTAACGGCAGATCGGTAATCGCCCGCAGATGCAGCTCAAACTGGCTGATGCTGGCACCGTTTTGTGTCCAGTGACCGCTGTTATGCACACGCGGTGCCAGTTCGTTGATCAACAGACCTTGCGGGGTGACAAAACACTCCATCGCCATCACGCCCACATAGCCCAGCTCCTGCATAATCGCCGACAGCATCTCTTCGGCTTGCGCCTGCTGCTGAGCGTTGGCCTg         >  1:99401/1‑251 (MQ=255)
                                                                                                                                                                                                                        aCGGATTATTCACCACTGGTTGTGGTAACGGCCGATCGGTAATCGCCCGCAGATGCAGCTCAAACTGGCTGATGCTGGCACCGTTTTGTGTCCAGTGACCGCTGTTATGCACACGCGGTGCCAGTTCGTTGATCAACAGACCTTGCGGGGTGACAAAACACTCCATCGACATCACGCCCACATAGCCCAGCTCCTGCATAATCGCCGACAGCATCTCTTCGGCTTGCGCCTGCTGCTGTGCGTTGGCCTGc        >  1:135143/1‑251 (MQ=255)
                                                                                                                                                                                                                        aCGGATTATTCACCACTGGTTGTGGTAACGGCAGATCGGTAATCGCCCGCAGATGCAGCTCAAACTGGCTGATGCTGGCACCGTTTTGTGTCCAGTGACCGCTGTTATGCACACGCGGTGCCAGTTCGTTGATCAACAGACCTTGCGGGGTGACAAAACACTCCATCGCCATCACGCCCACATAGCCCAGCTCCTGCATAATCGCCGACAGCATCTCTTCGGCTTGCGACTGCTGCTGTGCGTTGGCCTGc        >  2:32081/1‑251 (MQ=255)
                                                                                                                                                                                                                          ggATTATTCACCACTGGTTGTGGTAACGGCAGATCGGTAATCGCCCGCAGATGCAGCTCAAACTGGCTGATGCTGGCACCGTTTTGTGTCCAGTGACCGCTGTTATGCACACGCGGTGCCAGTTCGTTGATCAACAGACCTTGCGGGGTGACAAAACACTCCATCGCCATCACGCCCACATAGCCCAGCTCCTGCATAATCGCCGACAGCATCTCTTCGGCTTGCGCCTGCTGCTGTGCGTTGGCCTGCgg      >  1:169743/1‑251 (MQ=255)
                                                                                                                                                                                                                               attCACCACTGGTTGTGGTAACGGCAGATCGGTAATCGCCCGCAGATGCAGCTCAAACTGGCTGATGCTGGCACCGTTTTGTGTCCAGTGACCGCTGTTATGCACACGCGGTGCCAGTTCGTTGATCAACAGACCTTGCGGGGTGACAAAACACTCCATCGCCATCACGCCCACATAGCCCAGCTCCTGCATAATCGCCGACAGCATCTCTTCGGCTTGCGCCTGCTGCTGTGCGTTGGCCTGCGGaaaa  <  1:199676/250‑1 (MQ=255)
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                          
TCTGCGCCCAAATCACGCCGCTGGCATATTCCGGCGGCAGCAGCGGGATTAAGGCTTCCAGCGTCGCAGTCAGACGCGATGTGTCGCTGTCGGTCAAATTCAGATGCCCCACTTTACGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCACCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGATTATTCACCACTGGTTGCGGTAACGGCAGATCGGTAATCGCCCGCAGATGCAGCTCAAACTGGCTGATGCTGGCACCGTTTTGTGTCCAGTGACCGCTGTTATGCACACGCGGTGCCAGTTCGTTGATCAACAGACCTTGCGGGGTGACAAAACACTCCATCGCCATCACGCCCACATAGCCCAGCTCCTGCATAATCGCCGACAGCATCTCTTCGGCTTGCGCCTGCTGCTGTGCGTTGGCCTGCGGAAAA  >  NC_000913/551541‑552013

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑