Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	329,921	G→A	M153I (ATG→ATA)	betT →	choline:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	329,921	0	G	A	100.0%	99.4 / NA	32	M153I (ATG→ATA)	betT	choline:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/13);  total (19/13)

GTATCGCTTGTTCGCGTTTTGGTTCGGTGAAGCTCGGGCCAGAACAATCCAAACCGGAATTCAGCCTGCTGAGTTGGGCGGCGATGCTGTTTGCTGCCGGGATCGGTATCGACCTGATGTTCTTCTCCGTAGCCGAACCGGTAACGCAGTATATGCAGCCGCCGGAAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATGTATGCGCTGATGGGCATGGCGCTCGGATACTTTAGCTATCGTTATAATTTGCCGCTCACCATCCGCTCGGCGCTGTACCCGATCTTCGGTAAACGGATTAACGGGCCGATAGGTCACTCAGTGGATATTGCAGCGGTGATCGGCACTATCTTCGGTATTGCCACTACGCTCGGTATCGGTGTGGTGCAGCTTAACTATGGCTTGAGCGTACTGTTTGATATTCCCGATTCGATGGCGGCGAAA  >  NC_000913/329674‑330164                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                    gTATCGCTTGTTCGCGTTTTGGTTCGGTGAAGCTCGGGCCAGAACAATCCAAACCGGAATTCAGCCTGCTGAGTTGGGCGGCGATGCTGTTTGCTGCCGGGATCGGTATCGACCTGATGTTCTTCTCCGTAGCCGAACCGGTAACGCAGTATATGCAGCCGCCGGAAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGatatat                                                                                                                                                                                                                                                  >  1:67833/1‑251 (MQ=255)                                    ggaccAGAACAATCCAAACCTGAGTTAAGACTGCTAAGTAGGGCGGCGGTGCTGATTGCTGCCGGGATCGGTCTCGACCTGCTGTTCTTCTCCGTATCCGAACGGGTAACGCAGTATATGCAGCCGCCGGCAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGATGGTCTGGAAGCTGTTTCACTACGGCTTAACCGGCTGGACGCTATATGCGCTGATGGGCCTGGCGCTCGGCTACTTTAGCt                                                                                                                                                                                                                >  2:162615/4‑250 (MQ=255)                                       ccAGAACAATCCAAACCGGAATTCAGCCTGCTGAGTTGGGCGGCGATGCTGTTTGCTGCCGGGATCGGTATCGACCTGATGTTCTTCTCCGTAGCCGAACCGGTAACGCAGTATATGCAGCCGCCGGAAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATATATGCGCTGATGGGCATGGCGCTCGGATACTTTAGCTATCg                                                                                                                                                                                                            >  1:283019/1‑251 (MQ=255)                                               aTCCAAACCGGAATTCAGCCTGCTGAGTTGGGCGGCGATGCTGTTTGCTGCCGGGATCGGTATCGACCTGATGTTCTTCTCCGTAGCCGAACCGGTAACGCAGTATATGCAGCCGCCGGAAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATATATGCGCTGATGGGCATGGCGCTCGGATACTTTAGCTATCGTTATAAtt                                                                                                                                                                                                    <  1:140096/251‑1 (MQ=255)                                                             tCAGCCTGCTGAGTTGGGCGGCGATGCTGTTTGCTGCCGGGATCGGTATCGACCTGATGTTCTTCTCCGTAGCCGAACCGGTAACGCAGTATATGCAGCCGCCGGAAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATATATGCGCTGATGGGCATGGCGCTCGGATACTTTAGCTATCGTTATAATTTGCCGCTCACCATc                                                                                                                                                                                      >  2:200309/1‑251 (MQ=255)                                                                 ccTGCTGAGTTGGGCGGCGATGCTGTTTGCTGCTGGGATCGGTATCGACCTGATGTTCTTCTCCGTAGCCGAACCGGTAACGCAGTATATGCAGCCGCCGGAAGGCGCGGGACAGACGAGTGAGGCCGCGCGTCAGGCGATGGTCTGTACGCTGGTTCACTACGGCTTAACCGGCTGGTCGATATATGCGCTGATGGGCATGGCGGTGGGATACGTTAGCTATCGTTATAATTTGCCGCTCACCAGCCGCt                                                                                                                                                                                  <  2:283332/251‑1 (MQ=255)                                                                    gctgAGTTGGGCGGCGATGCTGTTTGCTGCCGGGATCGGTATCGACCTGATGTTCTTCTCCGTAGCCGAACCGGTAACGCAGTATATGCAGCCGCCGGAAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATATATGCGCTGATGGTCATGGCGCTCGGATACTTTAGCTATCGTTATAATTTGCCGCTCACCATCCGCTc                                                                                                                                                                                 <  1:50444/249‑1 (MQ=255)                                                                                      gCTGTTTGCGGCCGGGATCGGTATCGACCTGATGTTCTTCTCCGTAGCCGAACCGGTAACGCAGTATATGCAGCCGCCGGAAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATATATGCGCTGATGGGCATGGCGCTCGGATACTTTAGCTATCGTTATAATTTGCCGCTCACCATCCGCTCGGCGCTGTACCCGATCTTc                                                                                                                                                              <  2:67833/250‑1 (MQ=255)                                                                                                 ccGGGATCGGTATCGACCTGATGTTCTTCTCCGTAGCCGAACCGGTAACGCAGTATATGCAGCCGCCGGAAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATATATGCGCTGATGGGCATGGCGCTCGGATACTTTAGCTATCGTTATAATTTGCCGCTCACCATCCGCTCGGCGCTGTACCCGATCTTCGGTAAACGGAtt                                                                                                                                                  >  1:233486/1‑251 (MQ=255)                                                                                                         ggTATCGACCTGATGTTCTTCTCCGTAGCCGAACCGGTAACGCAGTATATGCAGCCGCCGGAAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATATATGCGCTGATGGGCATGGCGCTCGGTTACTTTAGCTATCGTTATAATTTGCCGCTCACCATCCGCTCGGCGCTGTACCCGATCTTCGGTAAACGGATTAACGGGc                                                                                                                                           >  2:196484/1‑250 (MQ=255)                                                                                                         ggTATCGACCTGATGTTCTTCTCCGTAGCCGAACCGGTAACGCAGTATATGCAGCCGCCGGAAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATATATGCGCTGATGGGCATGGCGCTCGGATACTTTAGCTATCGTTATAATTTGCCGCTCACCATCCGCTCGGCGCTGTACCCGATCTTCGGTAAACGGATTAACGGGc                                                                                                                                           <  1:196484/250‑1 (MQ=255)                                                                                                            atagACCTGATGTGCTTCTCCGACGCCGAACCGGTAACGCACTATCTGCAGCCGCCGGAAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAAGCGGCTGGTCGATATATGCGCTGATGGGCATGGCGCTCGGATACTTTAGCTATCGTTATAATTTGCCGCTCACCATCCGCTCGGCGCTGTACACGATCTTCGGTAAACGGATTAACGGGACGAt                                                                                                                                       >  2:117680/4‑251 (MQ=255)                                                                                                                cccTGATGTTCTTCTCCGTAGCCGAACCGGTAACGCAGTATATGCAGCCGCCGGAAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATATATGCGCTGATGGGCATGGCGCTCGGATACTTTAGCTATCGTTATAATTTGCCGCTCACCATCCGCTCGGCGCTGTACCCGATCTTCGGTAAACGGATTAACGGGCCGATAGGt                                                                                                                                   <  2:149253/250‑1 (MQ=255)                                                                                                                                     ccGAACCGGTAACGCAGTATATGCAGCCGCCGGAAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATATATGCGCTGATGGGCATGGCGCTCGGATACTTTAGCTATCGTTATAATTTGCCGCTCACCATCCGCTCGGCGCTGTACCCGATCTTCGGTAAACGGATTAACGGGCCGATAGGTCACTCAGTGGATATTGCAGCg                                                                                                              >  2:47720/1‑251 (MQ=255)                                                                                                                                       gAACCGGTAACGAAGTATATGCAGCCGCCGGAAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCCGTTTCACTACGGCTTAACCGGCTGGTCGATATATGCGCTGATGGGCATGGCGCTCGGATACTTTAGCTATCGTTATAATTTGCCGCTCACCATCCGCTCGGCGCTGTACCCGATCTTCGGTAAACGGATTAACGGGCCGATAGGTCACTCAGTGGATATTGCAgaggg                                                                                                            >  2:127574/1‑247 (MQ=255)                                                                                                                                         aCCGGTAACGCGGTATATGCAGCCGCCGGAAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCCCTGCGGCTTAACCGGCTGGTCGATATATGCGCTGATGGGCATGGCGCTCGGATACTTTAGCTATCGTTATAATTTGCCGCTCACCATCCGCTCGGCGCTGTACCCGATCTTCGGTAAACGGATTAACGGGCCGATAGGTCACTCAGTGGATATTGCAGCGGTGa                                                                                                          <  2:230534/251‑1 (MQ=255)                                                                                                                                            ggaaaCGCAGTATATGCAGCCGCCGGAAGGCGCGGGACAGACGAACGAGGCAGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATATATGCGCTGATGGGCATGGCGCTCGGATACTTTAGATATCGTTATAATTTGCCGCTCACCATCCGCTCGGCGCTGTACCCGATCTTCGGTAAACGGATTAACGGGCCGATAGGTCACTCAGTGGATATTGCAGCGGTGagcg                                                                                                       >  2:41059/4‑248 (MQ=255)                                                                                                                                            ggTAACGCAGTATATGCAGCCGCCGGAAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATATATGCGCTGATGGGCATGGCGCTCGGATACTTTAGCTATCGTTATAATTTGCCGCTCACCATCCGCTCGGCGCTGTACCCGATCTTCGGTAAACGGATTAACGGGCCGATAGGTCACTCAGTGGATATTGCAGCGGTGATCg                                                                                                       >  1:141996/1‑251 (MQ=255)                                                                                                                                                            cAGCCGCCGGAAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATATATGCGCTGATGGGCATGGCGCTCGGATACTTTAGCTATCGTTATAATTTGCCGCTCACCATCCGCTCGGCGCTGTACCCGATCTTCGGTAAACGGATTAACGGGCCGATAGGTCACTCAGTGGATATTGCAGCGGTGATCGGCACTATCTTCGGTAt                                                                                       <  2:184211/251‑1 (MQ=255)                                                                                                                                                                   cgGAAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATATATGCGCTGATGGGCATGGCGCTCGGATACTTTAGCTATCGTTATAATTTGCCGCTCACCATCCGCTCGGCGCTGTACCCGATCTTCGGTAAACGGATTAACGGGCCGATAGGTCACTCAGTGGATATTGCAGCGGTGATCGGCACTATCTTCGGTATTGCCACt                                                                                >  2:204999/1‑251 (MQ=255)                                                                                                                                                                     gaatgcgcGGGAAAGAAGAGTGCGGCCGCGCGTCAGGCGATGGACTGGACGCTGTTTCACTACGGCTTAACCGGCGGGTCGATATATGCGCGGATGGGCATGGCGCTCGGGTACTTTAGCTAGCGTTATAATGTGCCGCTCACCATCCGCTCGGCGCTGTACCCGATCTTCGGTAAACGGCGTAACGGGCCGATAGGTCACTCAGTGGATATTGAAGCGGTGATCGGAGCTATCTTCGGTACTGCCACTAc                                                                              >  2:32220/5‑251 (MQ=255)                                                                                                                                                                             gggACAGACGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATATATGCGCTGATGGGCATGGCGCTCGGATACTTTAGCTATCGTTATAATTTGCCGCTCACCATCCGCTCGGCGCTGTACCCGATCTTCGGTAAACGGATTAACGGGCCGATAGGTCACTCAGTGGATATTGCAGCGGTGATCGGCACTATCTTCGGTATTGCCACTACGCTCGGt                                                                       >  1:38066/1‑250 (MQ=255)                                                                                                                                                                               gACAGACGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATATATGCGCTGATGGGCATGGCGCTCGGATACTTTAGCTATCGTTATAATTTGCCGCTCACCATCCGCTCGGCGCTGTACCCGATCTTCGGTAAACGGATTAACGGGCCGATAGGTCACTCAGTGGATATTGCAGCGGTGATCGGCACTATCTTCGGTATTGCCACTACGCTCGGTATc                                                                    <  1:71340/251‑1 (MQ=255)                                                                                                                                                                                       aTTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTGACTACGGCTTAACCGGCTGGTCGATATATGCGCTGATGGGCATTGCGTTCGGATACTTTAGCTATCGTTATAATTTGCCGGTCACCATGCGCTCGGCGCTGTACCCGATCTTCGGGAAACGGATTAACGGGCCGATAGGTCACTCAGTGGATATTGCAGCGGTGATCGGCTCTATCTTCGGTATGGCCGCTACGCTCGCTATCGTTgtggt                                                            <  2:24053/251‑1 (MQ=255)                                                                                                                                                                                            ggCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATATATGCGCTGATGGGCATGGCGCTCGGATACTTTAGCTATCGTTATAATTTGCCGCTCACCATCCGCTCGGCGCTGTACCCGATCTTCGGTAAACGGATTAACGGGCCGATAGGTCACTCAGTGGATATTGCAGCGGTGATCGGCACTATCTTCGGTATTGCCACTACGCTCGGTATCGGTGTGGTGCAGc                                                       >  2:148940/1‑251 (MQ=255)                                                                                                                                                                                                      cAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATATATGCGCTGATGGGCATGGCGCTCGGATACTTTAGCTATCGTTATAATTTGCCGCTCACCATCCGCTCGGCGCTGTACCCGATCTTCGGTAAACGGATTAACGGGCCGATAGGTCACTCAGTGGATATTGCAGCGGTGATCGGCACTATCTTCGGTATTGCCACTACGCTCGGTATCGGTGTGGGGCAGCTTAACTATgg                                             >  2:8002/1‑251 (MQ=255)                                                                                                                                                                                                            aTGGGCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATATATGCGCTGATGGGCATGGCGCTCGGATACTTTAGCTATCGTTATAATTTGCCGCTCACCATCCGCTCGGCGCTGTACCCGATCTTCGGTAAACGGATTAACGGGCCGATAGGTCACTCAGTGGATATTGCAGCGGTGATCGGCACTATATTCGGTATTGCCACTACGCTCGGTATCGGTGTGGTGCAGCTTAACTATGGCTTg                                         >  1:147154/1‑249 (MQ=255)                                                                                                                                                                                                               gTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATATATGCGCTGATGGGCATGGCGCTCGGATACTTTAGCTATCGTTATAATTTGCCGCTCACCATCCGCTCGGCGCTGTACCCGCTCTTCGGTAAACGGATTAACGGGCCGATAGGTCACTCAGTGGATATTGCAGCGGTGATCGGCACTATCTTCGGTATTGCCACTACGCTCGGTATCGGTGGGGCGCAGCTTAACTATGGcctgcgcgc                                    >  2:105842/1‑243 (MQ=255)                                                                                                                                                                                                                            ttCACTACGGCTTAACCGGCTGGTCGATATATGCGCTGATGGGCATGGCGCTCGGATACTTTAGCTATCGTTATAATTTGCCGCTCACCATCCGCTCGGCGCTGTACCCGATCTTCGGTAAACGGATTAACGGGCCGATAGGTCACTCAGTGGATATTGCAGCGGTGATCGGCACTATCTTCGGTATTGCCACTACGCTCGGTATCGGTGTGGTGCAGCTTAACTATGGCTTGAGCGTACTGTTTGatat                        <  2:50113/250‑1 (MQ=255)                                                                                                                                                                                                                               aCTACGGCTTAACCGGCTGGTCGATATATGCGCTGATGGGCATGGCGCTCGGATACTTTAGCTATCGTTATAATTTGCCGCTCACCATCCGCTCGGCGCTGTACCCGATCTTCGGTAAACGGATTAACGGGCCGATAGGTCACTCAGTGGATATTGCAGCGGTGATCGGCACTATCTTCGGTATTGCCACTACGCTCGGTATCGGTGTGGTGCAGCTTAACTATGGCTTGAGCGTACTGTTTGATATTccc                    >  1:220825/1‑251 (MQ=255)                                                                                                                                                                                                                                        tAACCGGCTGGACGATATATGCTCTGTTGTGCATGGCGCTCGGATACTTTAGCTATCGTTATAATTTGCCGCTCACCCTCCGCTCGGCGCTGTACCCGATCTTCGGTAAACGGATTAACGGGCCGATAGGTCACTCAGCGGATATTGCAGCGGTGATCGGCACTATCTTCGGTATTGCCCCTACGCCCGGTATCGGTGTGGTGCAGCTTAACTATGGCTTGAGCGTACTGTTTGATATTCCCGATTCNATg           >  2:257075/1‑251 (MQ=255)                                                                                                                                                                                                                                        tAACAGGCTGGTCGATATATGCCCTGATGGGCATGGCGCTCGGATACTTTAGCTATCGTTATAATTTGCCGTTCACCATCCTTTCGGCTTTGTACCCGACCTTCGGTAAACGTATTAACGGGCCGACAGGTCTCTCAGTGGACATTGCATCGGTGATCTGCACTATCTTCGGTATTGCCATTCCGCTCGGTATCGGTTTGGTGCAGCTTAACTATGGATTGAGCGTACTGTTTGATATTCCCGATTCGAt            <  1:162615/250‑1 (MQ=255)                                                                                                                                                                                                                                                 ggTCGATATATGCGCTGATGGGCATGGCGCTCGGATACTTTAGCTATCGTTATAATTTGCCGCTCACCATCCGCTCGGCGCTGTACCCGATCTTCGGTAAACGGATTAACGGGCCGATAGGTCACTCAGTGGATATTGCAGCGGTGATCGGCACTATCTTCGGTATTGCCACTACGCTCGGTATCGGTGTGGTGCAGCTTAACTATGGCTTGAGCGTACTGTTTGATATTCCCGATTCGATGGCGGCGaaa  <  2:107425/251‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                    GTATCGCTTGTTCGCGTTTTGGTTCGGTGAAGCTCGGGCCAGAACAATCCAAACCGGAATTCAGCCTGCTGAGTTGGGCGGCGATGCTGTTTGCTGCCGGGATCGGTATCGACCTGATGTTCTTCTCCGTAGCCGAACCGGTAACGCAGTATATGCAGCCGCCGGAAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATGTATGCGCTGATGGGCATGGCGCTCGGATACTTTAGCTATCGTTATAATTTGCCGCTCACCATCCGCTCGGCGCTGTACCCGATCTTCGGTAAACGGATTAACGGGCCGATAGGTCACTCAGTGGATATTGCAGCGGTGATCGGCACTATCTTCGGTATTGCCACTACGCTCGGTATCGGTGTGGTGCAGCTTAACTATGGCTTGAGCGTACTGTTTGATATTCCCGATTCGATGGCGGCGAAA  >  NC_000913/329674‑330164

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑