Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	631,633	G→A	W580* (TGG→TGA)	cstA →	pyruvate transporter CstA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	631,633	0	G	A	100.0%	84.9 / NA	26	W580* (TGG→TGA)	cstA	pyruvate transporter CstA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/17);  total (9/17)

GCTGGGCGTGGTGTCTCCTGGCCTGAAGCGGACCGATTCACTGCCTGCTAACCTGCTGGCAACAGCGCTGTGCGTACTGGCGTGGGGCTACTTCCTCCATCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTCAAGATGAAACGTCAACGTTACGCCTGGGTGGCGCTGGTACCAACGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGCCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCTGGCGCAACTGGTGTTCAACAACCGTCTGGATGCCGGGTTAACCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTT  >  NC_000913/631396‑631878                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                      gctgGGCGTGGTGTCTCCTGGCCTGAAGCGGACCGATTCACTGCCTGCTAACCTGCTGGCAACAGCGCTGTGCGTACTGGCGTGGGGCTACTTCCTCCATCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTCAAGATGAAACGTCAACGTTACGCCTGAGTGGCGCTgg                                                                                                                                                                                                                                             <  1:242225/248‑1 (MQ=255)                           aGCGGACCGACTCACTGCCTGGTAACCTGCTGGCAACAGCGCTGTGCGTACTGGCGTGGGGCTACTTCCTCCAGCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACTCTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTTGTGTTGTTCAAGATGAAACGGCAACGTTACGCCTGAGTGGCGCTGGTACCAACGTCCTGGCTGCTGATTTTTAcc                                                                                                                                                                                                                <  1:29035/251‑1 (MQ=255)                              ggACCGATTCACTGCCTGCTAACCTGCTGGCAACAGCGCTGTGCGTACTGGCGTGGGGCTACTTCCTCCATCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGGTCAAGATGAAACGTCAACGTTACGCCTGAGTGGCGCTGGTACCAAAGGCCTGGCTGCTGATTTGTACCCTg                                                                                                                                                                                                             >  1:8670/1‑251 (MQ=255)                                 ccGATTCACTGCCTGCTAACCTGCTGGCATCAGCGCTGTGCGTACTGGCGTGGGGCTACTTCCTCCATCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTCAAGATGAAACGTCAACGTTACGCCTGAGTGGCGCTGGTACCAACGGCCTGGCTGCTGATTTGTACCCTGAcc                                                                                                                                                                                                          <  2:122008/251‑1 (MQ=255)                                                                               ggCGTGGGGCTACTTCCTCCATCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTCAAGATGAAACGTCAACGTTACGCCTGAGTGGCGCTGGTACCAACGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTc                                                                                                                                                             <  1:222988/250‑1 (MQ=255)                                                                                    ggggCTACTTCCTCCATCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTCAAGATGAAACGTCAACGTTACTCCTGAGTGGCGCTGGTACCAACGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTgtcc                                                                                                                                                       <  1:132141/251‑4 (MQ=255)                                                                                        cTACTTCCTCCATCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTCAAGATGAAACGTCAACGTTACGCCTGAGTGGCGCTGGTACCAACGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCTCGGATGCGAAAGTCGGCTTCCTGGCCATTg                                                                                                                                                   >  1:138855/1‑251 (MQ=255)                                                                                                  cATCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTCAAGATGAAACGTCAACGTTACGCCTGAGTGGCGCTGGTACCAACGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGCCATTGCTAATAAGtt                                                                                                                                         <  1:211130/251‑1 (MQ=255)                                                                                                                    ccGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTCAAGATGAAACGTCAACGTTACGCCTGAGTGGCGCTGGTACCAACGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGCCATTGCTAATAAGTTCCAGGCAATGATCGACa                                                                                                                        <  1:24082/250‑1 (MQ=255)                                                                                                                    ccGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTCAAGATGAAACGTCAACGTTACGCCTGAGTGGCGCTGGTACCAACGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGCCATTGCTAATAAGTTCCAGGCAATGATCGACAg                                                                                                                       <  2:51976/251‑1 (MQ=255)                                                                                                                         gggCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTCAAGATGAAACGTCAACGTTACGCCTGAGTGGCGCTGGTACCAACGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGGCGGCTTCCTGGCCATTGCTAATAAGTTCCAGGCAATGATCGACAGCgg                                                                                                                    <  2:126229/249‑1 (MQ=255)                                                                                                                            gggcATTAACACTCTGTGGCCGCTGTTTGGTATTGACAACCAGATGCTGGCAGGGATGGCGCTGATGCTCGGTGCCGTGGTGTTGTTCAAGATGAAACGTCAACGTTACGCCTGAGTGGCGCTGGTACCAACGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGCCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAata                                                                                                               <  1:118179/250‑1 (MQ=255)                                                                                                                                   aaCACTCTGTGGCCGCTGTTTGGAATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGTTGTTGTTCAAGATGAAACGTCAACGTTACGCCTGAGTGGCGCGGGTACCAACGTCCTGGCTGCTTATTTGTACCCTGACCGCAGGCTGGCAAAAAGCGTTTTGCCCGGATGAGAAAGTCGGCTTCCTGGCCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTc                                                                                                        <  2:165378/251‑1 (MQ=255)                                                                                                                                              gCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGGCGTGGAGTTGTTCAAGATGAAACGTCAACGTTACGCCTGAGTGGGGCTGGTACCAACGGCCTGGCTGCTGATTTGTACCCTGAACGCAGGCTTGCAGAAAGCGTTTAGCCCGGATGCGAAAGGCGGCTTCCTGGCCATTGCTAATAAGTTCCAGGCAATGATTGACAGCGGCAATATTCCGTCGCAGTATACTg                                                                                             >  2:95320/1‑251 (MQ=255)                                                                                                                                                       tGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTCAAGATGAAACGTCAACGTTACGCCTGAGTGGCGCTGGTACCAACGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGCCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAg                                                                                     <  1:153173/250‑1 (MQ=255)                                                                                                                                                          tATTGCCAACCAGCTGCTGGCAGGGATGGCGCTGATGCTCTGTGCAGGGGTGTTGTTCAAGATGAAACGGCAACGTTACGCCTGAGTGGCGCTGGTACCAACGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGCCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTTCGTCGCAGTATATTGAGTCACAGCTgg                                                                                 >  2:137198/1‑251 (MQ=255)                                                                                                                                                           aTTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTCAAGATGAAACGTCAACGTTACGCCTGAGTGGCGCTGGTACCAACGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGCCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGTAATATTCCGTCGCAGTATACTGAGTCACAGCTGgc                                                                                <  1:107060/251‑1 (MQ=255)                                                                                                                                                                 aaCCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTCAAGATGAAACGTCAACGTTACGCCTGAGTGGCGCTGGTACCAACGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGCCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCTGGCGCAACt                                                                          >  1:163078/1‑251 (MQ=255)                                                                                                                                                                    cAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTCAAGATGAAACGTCAACGTTACGCCTGAGTGGCGCTGGTACCAACGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGCCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCTGGCGCAACTGgt                                                                       >  2:70476/1‑251 (MQ=255)                                                                                                                                                                               aGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTCAAGATGAAACGTCAACGTTACGCCTGAGTGGCGCTGGTACCAACGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGCCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCTGGCGCAACTGGTGTTCAACAAcc                                                            <  1:154327/251‑1 (MQ=255)                                                                                                                                                                                      gcgcTGATGCTCTGTGCCGGGGTGTTGTTCAAGATGAAACGTCAACGTTACGCCTGAGTGGCGCTGGTACCAACGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCTCGGATGCGAAAGTCGGCTTCCTGGCCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCTGGCGCAACTGGTGTTCAACAACAGTCTGGa                                                     <  2:138855/251‑1 (MQ=255)                                                                                                                                                                                                  tgtgCCGTGACGTTGTTCAAGATGAAACGTCAACGTTACGCCTGAGTGGCGCTGGTACCCACGGCCTGGCTGCTGATTTGAACCCTGACCGCAGGCTGGCAGAAAGCGTCTAGCCCGGATGCGAAAGTCGGCTTCCTGGCCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCTCAGTATCCCGAGTCAAAGCTGGCGCATCTGGTGTTCAACAACCGACTGGAACCCGGCTTAAc                                         >  2:144436/1‑251 (MQ=255)                                                                                                                                                                                                            tgttgtTCAAGATGAAACGTCAACGTTACGCCTGAGTGGCGCTGGAACCAACGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGATGGCAGAAAGGGTTTAGCCCGGAAGCGAAAGTCGGCTTCCTGGCCAGTGCTAATAAGATCCAGGCAATGATCGACAGCGGCAGTATTCCGTCGCAGTATACTGAGTCACAGCTCGCGCAACTGGTGTTCAACAACGATCTGGATGCCGGGTTAACCATCTTCttt                               >  1:115032/1‑251 (MQ=255)                                                                                                                                                                                                                   cAAGATGAAACGTCAACGTTACGCCTGAGTGGCGCTGGTACCAACGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGCCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCTGGCGCAACTGGTGTTCAACAACCGTCTGGATGCCGGGTTAACCATCTTCTTTAtggtg                         <  1:259938/250‑1 (MQ=255)                                                                                                                                                                                                                               tCAACGTTACGCCTGAGTGGCGCTGGTACCAACGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGCCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCTGGCGCAACTGGTGTTCAACAACCGTCTGGATGCCGGGTTAACCATCTTCTTTATGGTGGTCGTGGTGGttt            >  1:252365/1‑250 (MQ=255)                                                                                                                                                                                                                                         gCCTGAGTGGCGCTGGTACCAACGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGCCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCTGGCGCAACTGGTGTTCAACAACCGTCTGGATGCCGGGTTAACCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGtt  <  1:178388/251‑1 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                      GCTGGGCGTGGTGTCTCCTGGCCTGAAGCGGACCGATTCACTGCCTGCTAACCTGCTGGCAACAGCGCTGTGCGTACTGGCGTGGGGCTACTTCCTCCATCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTCAAGATGAAACGTCAACGTTACGCCTGGGTGGCGCTGGTACCAACGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGCCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCTGGCGCAACTGGTGTTCAACAACCGTCTGGATGCCGGGTTAACCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTT  >  NC_000913/631396‑631878

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑