Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	426,395	C→T	L87F (CTC→TTC)	tgt →	tRNA‑guanine transglycosylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	426,395	0	C	T	100.0%	114.0 / NA	33	L87F (CTC→TTC)	tgt	tRNA‑guanine transglycosylase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/20);  total (13/20)

CACCGACGGTCGCGCACGCCGTGGCCGCCTGGTCTTTGATCGTGGCGTAGTGGAAACGCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATCATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCTTGGTTCGGATATCGTCATGATCTTTGATGAGTGTACGCCGTATCCTGCTGACTGGGATTACGCAAAACGCTCCATGGAGATGTCTCTGCGTTGGGCGAAGCGT  >  NC_000913/426157‑426640                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                      caccGAAGGTCGCGCACGCCGTGGCCGCCTGGTCTTTGATCGTGGCGTAGTGGAAACGCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATCATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCTTCACCGACTCcg                                                                                                                                                                                                                                           >  1:88719/1‑251 (MQ=255)         gTCGCGCACGCCGTGGCCGCCTGGTCTTTGATCGTGGCGTAGTGGAAACGCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATCATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCTTCACCGACTCCGGCGGCTTc                                                                                                                                                                                                                                   <  2:46326/251‑1 (MQ=255)            gcgcACGCCGTGGCCGCCTGGTCTTTGATCGTGGCGTAGTGGAAACGCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATCATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCTTCACCGACTCCGGCGGCTTcc                                                                                                                                                                                                                                  >  1:74958/1‑249 (MQ=255)                            ccTGGTCTTTGATCTTGGCGTAGTGGAAACGCCTTGTTTTATGCCTGTTGGCACCGACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATCATGAAACTGCACGGCGATCTGCACGATTTTATTCAATGTAAGGGGCCGATCTTCACCGACTAcggcggaggaaaggggggaagaaggggag                                                                                                                                                                                                                <  1:241662/251‑24 (MQ=255)                            ccTGGTCTTTGATCGTGGCGTAGTGGAAACGCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATCATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCTTCACCGACTCCGGCGGCTTCCAGGTTTTCAGCCTTGGCg                                                                                                                                                                                                                <  1:97936/251‑1 (MQ=255)                                    ttGATCGTGGCGTAGTGGAAACGCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATCATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCTTCACCGACTCCGGCGGCTTCCAGGTTTTCAGCCTTGGCGATATTCg                                                                                                                                                                                                         <  2:5883/250‑1 (MQ=255)                                          gTGGCGTAGTGGAAACGCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATCATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCTTCACCGACTCCGGCGGCTTCCAGGTTTTCAGCCTTGGCGATATTCGTAAAATc                                                                                                                                                                                                  >  1:173516/1‑251 (MQ=255)                                           gggCGTAGTGGAAACGCCTTGTTTTATGCCTGTTGGCACCTACGGCACAGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATCATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCTTCACCGACTCCGGCGGCTTCCAGGTTTTCAGCCTTGGTGATATTCGTAAAATCa                                                                                                                                                                                                 <  1:245621/250‑1 (MQ=255)                                            ggctgaGTGGAAACGCCTTGTTTTAGGCCGGTTGGCCCCTCCGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATCATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCTTCACCGACTGCGGCGGCTTCCAGGTTTTCAGCCTTGGCGATATTCGTAAAATCAc                                                                                                                                                                                                <  2:276519/246‑1 (MQ=255)                                            ggCGTAGTGGAAACGCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATCATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCTTCACCGACTCCGGCGGCTTCCAGGTTTTCAGCCTTGGCGATATTCGTAAAATCAc                                                                                                                                                                                                >  1:98474/1‑251 (MQ=255)                                                     gAAACGCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATCATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCTTCACCGACTCCGGCGGCTTCCAGGTTTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAggg                                                                                                                                                                                       >  1:38340/1‑251 (MQ=255)                                                                        cTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACTCCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATCATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCTTCACCGACTCCGGCGGCTTCCAGGTTTTCAGCCTTGGCGATATTCg                                                                                                                                                                                                         <  1:279368/214‑1 (MQ=255)                                                                        cTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATCATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCTTCACCGACTCCGGCGGCTTCCAGGTTTTCAGCCTTGGCGATATTCg                                                                                                                                                                                                         >  2:279368/1‑214 (MQ=255)                                                                                        cACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATCATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCTTCACCGACTCCGGCGGCTTCCAGGTTTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTAACCCGATCAACGGCGATCCGa                                                                                                                                                    <  1:8570/251‑1 (MQ=255)                                                                                         aCCGTAAAAGGGATGAAGCCGGAAGAAGTTGATGCCACTGGCGCGCAAATTATCCTCGGCAACCCCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATCATGAATCTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCTTCACCGACTCCGGCGGCTTCCAGGTTTTCAGCCTTGGCGATATTCGTAAAAGCACCGAACAGGGCGTGCACTTCCGCAACCCGATCAAAGGCGATACGAt                                                                                                                                                   <  2:99208/251‑1 (MQ=255)                                                                                          ccGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATCATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCTTCACCGACTCCGGCGGCTTCCAGGTTTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTAACCCGATCAACGGCGATCCGAtt                                                                                                                                                  <  1:8482/251‑1 (MQ=255)                                                                                             tAAAAGGGATGACGCCGGAAGAAGTTGAATCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATCATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCTTCACCGACTCCGGCGGCTTCCAGGTTTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTAACCCGATCAACGGc                                                                                                                                                           >  2:21367/1‑239 (MQ=255)                                                                                             tAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTGCCACCTGTGGCTGCGCCCGGGCCAGGAAATCATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCTTCACCGACTCCGGCGGCTTCCAGGTTTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTAACCCGATCAACGGc                                                                                                                                                           <  1:21367/239‑1 (MQ=255)                                                                                                        aCGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATCATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCTTCACCGACTCCGGCGGCTTCCAGGTTTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGa                                                                                                                                    <  1:122536/251‑1 (MQ=255)                                                                                                         cGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATCATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCTTCACCGACTCCGGCGGCTTCCAGGTTTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGa                                                                                                                                    >  1:245468/1‑250 (MQ=255)                                                                                                             ggAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACTCCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATCATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCTTCACCGACTCCGGCGGCTTCCAGGTTTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAAt                                                                                                                               <  2:225185/251‑1 (MQ=255)                                                                                                                                       aaaTTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATCATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCTTCACCGACGCCGGCGGCTTCCAGGTTTTCAGCCTTGGCGATATGCGTAAAATCACCGAACAGGGCGTTCACTTCCGTAACCCGATCAACGTCGAGCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCGTGGt                                                                                                     <  2:38091/251‑1 (MQ=255)                                                                                                                                                 cGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATCATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCTTCACCGACTCCGGCGGCTTCCAGGTTTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCTTGGTTCGGatat                                                                                             <  2:38340/249‑1 (MQ=255)                                                                                                                                                        acCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATCATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCTTCACCGACTCCGGCGGCTTCCAGGTTTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCTTGGTTCGGATATCGTCATGAt                                                                                    >  1:98046/1‑251 (MQ=255)                                                                                                                                                               aCCTGTGGCTGCGCCCGGGCCAGGAAATCATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCTTCACCGACTCCGGCGGCTTCCAGGTTTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCTTGGTTCGGATATCGTCATGATCTTtgat                                                                             >  1:282932/1‑251 (MQ=255)                                                                                                                                                                               gggCCAGGAAATCATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCTTCACCGACTCCGGCGGCTTCCAGGTTTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCTTGGTTCGGATATCGTCATGATCTTTGATGAGTGTACGCCGTATc                                                             >  2:224649/1‑251 (MQ=255)                                                                                                                                                                                     ggAAAGCATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCTTCACCGACTCCGGCGGCTTCCAGGTATTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCTTGGTTCGGATATCGTCATGATCTTTGATGAGTGTACGCCGTTTCctgctg                                                       <  2:222285/251‑1 (MQ=255)                                                                                                                                                                                            aTGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCTTCACCGACTCCGGCGGCTTCCAGGTTTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCTTGGTTCGGATATCGTCATGATCTTTGATGAGTGTACGCCGTATCCTGCTGACTggg                                                 >  2:96969/1‑250 (MQ=255)                                                                                                                                                                                                 aCTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCTTCACCGACTCCGGCGGCTTCCAGGTTTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCTTGGTTCGGATATCGTCATGATCTTTGATGAGTGTACGCCGTATCCTGCTGACTGGGATTACg                                           <  2:117062/251‑1 (MQ=255)                                                                                                                                                                                                                gctcgtcgttATGAAGTGGATGGGGCCGATCTACACCGCCACCGGCGTCTTCCAGGTTTTCAGCCTTGGCGATATTCTTAAAATCACCGAACAGGGCGTGCACTTCCGTAACCCTATCAACGGCGATCCGATTTTCCTCGATCATGAAAAATCAATGGAGATTCAGTACGATCTTGGTTCGGAGATTGTCATGATCTTTGATGAGTGTACGCCGTATCCTGCTGACTGGGATTACGCAAAACGCt                                  <  2:51030/237‑1 (MQ=255)                                                                                                                                                                                                                gCACGATTTTATGCAGTGGAAGGGGCCGATCTTCACCGACTCCGGCGGCTTCCAGGTTTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCTTGGTTCGGATATCGTCATGATCTTTGATGAGTGTACGCCGTATCCTGCTGACTGGGATTACGCAAAACGCTcc                                <  2:98046/247‑1 (MQ=255)                                                                                                                                                                                                                                        gCCGATCTTCACCGACTCCGGCGGCTTCCAGGTTTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCTTGGTTCGGATATCGTCATGATCTTTGATGAGTGTACGCCGTATCCTGCTGACTGGGATTACGCAAAACGCTCCATGGAGATGTCTCTGCGTTGGGCGAAGc    <  1:147330/251‑1 (MQ=255)                                                                                                                                                                                                                                           gATCTTCACCGACTCCGGCGGCTTCCAGGATTTCATAATTGCCGATACTAGTAAAATCACCGAACAGGGCGTGCACTTCCGTAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGACAAATCAATGGCGATTCAGTACGATCTTGGTTCGGATATCGTCATGATCTTTGTTGAGTGTACGCCGTATCCTGCTGACTGGGATTACGCAAAACGCTCCATGGAGATGTCTCTGCGTTGGGCGAAGCGt  >  1:154008/1‑250 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                      CACCGACGGTCGCGCACGCCGTGGCCGCCTGGTCTTTGATCGTGGCGTAGTGGAAACGCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATCATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCTTGGTTCGGATATCGTCATGATCTTTGATGAGTGTACGCCGTATCCTGCTGACTGGGATTACGCAAAACGCTCCATGGAGATGTCTCTGCGTTGGGCGAAGCGT  >  NC_000913/426157‑426640

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑