Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,228,994	G→A	G36D (GGC→GAC)	ygjK →	glycoside hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,228,994	0	G	A	94.4%	107.9 / ‑3.9	37	G36D (GGC→GAC)	ygjK	glycoside hydrolase
Reads supporting (aligned to +/- strand):  ref base G (0/2);  new base A (16/18);  total (16/21)
Fisher's exact test for biased strand distribution p-value = 4.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

ACGAATACGGCGGCGGACGTTTCGCCACGGCTTTTGATAGCCGCTTCTACATGAAAAATGCCTCTCACGATCCCAGCGACCAAATCTTCCTGATGCAATATTTCTACTGGTAATAAGGACTGTAATTTCATGAAAATAAAAACTATTTTAACGCCAGTAACCTGCGCTCTGCTGATAAGTTTTTCCGCCCATGCCGCTAACGCCGACAA‑TTATAAAAACGTGATTAACCGTACTGGCGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGTACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACTTTACGCTGGAGGCATACAGTATTCCCG  >  NC_000913/3228759‑3229236                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                   aCGAATACGGCGGCGGACGTTTCGCCACGGCTTTTGATAGCCGCTTCTACATGAAAAATGCCTCTCACGATCCCAGCGACCAAATCTTCCTGATGCAATATTTCTACTGGTAATAAGGACTGTAATTTCATGAAAATAAAAACTATTTTAACGCCAGTAACCTGCGCTCTGCTGATAAGTTTTTCCGCCCATGCCGCTAACGCCGACAA‑TTATAAAAACGTGATTAACCGTACTGACGCGCCGCAGTACa                                                                                                                                                                                                                                      <  1:186286/250‑1 (MQ=255)         ggcggcggACGGTTCGCCACGGCTTTTGATAGCCGCTTCTACATGAAAAATGCCTCTCACGATCCCAGCGACCAAATCTTCCTGATGCAATATTTCTACTGGTAATAAGGACTGTAATTTCATGAAAATAAAAACTATTTTAACGCCAGTAACCTGCGCTCTGCTGATAAGTTTTTCCGCCCATGCCGCTAACGCCGACAA‑TTATAAAAACGTGAGTAACCGTACTGACGCGCCGCAGTACATGAAGgatt                                                                                                                                                                                                                             <  1:80618/251‑1 (MQ=255)           cggcggACGTTTCGC‑ACGGCTTTTGATAGCCGCTTCTACATGAAAAATGCCTCTCACGATCCCAGCGACCAAATCTTCCTGATGCAATATTTCTACTGGTAATAAGGACTGTAATTTCATGAAAATAAAAACTATTTTAACGCCAGTAACCTGCGCTCTGCTGATAAGTTTTTCCGCCCATGCCGCTAACGCCGACAA‑TTATAAAAACGTGATTAACCGTACTGACGCGCCGCAGTACATGAAGgattacg                                                                                                                                                                                                                          >  2:81427/1‑251 (MQ=255)              ggggcgttgcgcacaggCTTTGGATAGCCGGTTTTACAAGAAAAATGCCTCCCATGAACCCAGCCGCCAAAAGTTCCTGATGCAATATTTCTACTGGTAATAAGGACTCTAAATTCATGAAAAGAAAAATTTTTTTAACGCCAGTAACCTGCGTTCTGCTGATAATTTTTTCCGCCCATGCCGCTAACGTCGACAA‑TTATAAAAACGTGATAATCCGTACTGGCGCGCCGCGGTACATGAAAgattacgat                                                                                                                                                                                                                        <  2:38930/236‑1 (MQ=255)                       cGCCACGGCTTTTGATAGCCGCTTCTACATGAAAAATGCCTCTCACGATCCCAGCGACCAAATCTTCCTGATGCAATATTTCTACTGGTAATAAGGACTGTAATTTCATGAAAATAAAAACTATTTTAACGCCAGTAACCTGCGCTCTGCTGATAAGTTTTTCCGCCCATGCCGCTAACGCCGACAA‑TTATAAAAACGTGATTAACCGTACTGACGCGCCGCAGTACATGAAGGATTACGATTACGACGAt                                                                                                                                                                                                               >  2:102646/1‑251 (MQ=255)                                   tGATAGCCGCTTCTACATGAAAAATGCCTCTCACGATCCCAGCGACCAAATCTTCCTGATGCAATATTTCTACTGGTAATAAGGACTGTAATTTCATGAAAATAAAAACTATTTTAACGCCAGTAACCTGCGCTCTGCTGATAAGTTTTTCCGCCCATGCCGCTAACGCCGACAA‑TTATAAAAACGTGATTAACCGTACTGACGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGttgt                                                                                                                                                                                                   >  1:255139/1‑249 (MQ=255)                                      tAGCCGCTTCTACATGAAAAATGCCTCTCACGATCCCAGCGACCAAATCTTCCTGATGCAATATTTCTACTGGTAATAAGGACTGTAATTTCATGAAAATAAAAACTATTTTAACGCCAGTAACCTGCGCTCTGCTGATAAGTTTTTCCGCCCATGCCGCTAACGCCGACAA‑TTATAAAAACGTGATTAACCGTACTGACGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAAt                                                                                                                                                                                                >  2:178543/1‑251 (MQ=255)                                                 aCATGAAAAATGCCTCTCACGATCCCAGCGACCAAATCTTCCTGATGCAATATTTCTACTGGTAATAAGGACTGTAATTTCATGAAAATAAAAACTATTTTAACGCCAGTAACCTGCGCTCTGCTGATAAGTTTTTCCGCCCATGCCGCTAACGCCGACAA‑TTATAAAAACGTGATTAACCGTACTGACGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTc                                                                                                                                                                                       <  1:19949/249‑1 (MQ=255)                                                  cATGAAAAATGCCTCTCACGATCCCAGCGACCAAATCTTCCTGATGCAATATTTCTACTGGTAATAAGGACTGTAATTTCATGAAAATAAAAACTATTTTAACGCCAGTAACCTGCGCTCTGCTGATAAGTTTTTCCGCCCATGCCGCTAACGCCGACAA‑TTATAAAAACGTGATTAACCGTACTGACGCGCCGCGGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGAt                                                                                                                                                                                    <  1:251050/251‑1 (MQ=255)                                                         aaTGCCTCTCACGATCCCAGCGACCAAATCTTCCTGATGCAATATTTCTACTGGTAATAAGGACTGTAATTTCATGAAAATAAAAACTATTTTAACGCCAGTAACCTGCGCTCTGCTGATAAGTTTTTCCGCCCATGCCGCTAACGCCGACAA‑TTATAAAAACGTGATTAACCGTACTGACGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGATCTCgg                                                                                                                                                                               >  1:79769/1‑249 (MQ=255)                                                             ccTCTCACGATCCCAGCGACCAAATCTTCCTGATGCAATATTTCTACTGGTAATAAGGACTGTAATTTCATGAAAATAAAAACTATTTTAACGCCAGTAACCTGCGCTCTGCTGATAAGTTTTTCCGCCCATGCCGCTAACGCCGACAA‑TTATAAAAACGTGATTAACCGTACTGACGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGATCTCGGAGCCTg                                                                                                                                                                         <  1:191932/251‑1 (MQ=255)                                                                  cACGATCCCAGCGACCAAATCTTCCTGATGCAATATTTCTACTGGTAATAAGGACTGTAATTTCATGAAAATAAAAACTATTTTAACGCCAGTAACCTGCGCTCTGCTGATAAGTTTTTCCGCCCATGCCGCTAACGCCGACAA‑TTATAAAAACGTGATTAACCGTACTGACGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGATCTCGGAGCCTGGCATg                                                                                                                                                                    >  1:176571/1‑251 (MQ=255)                                                                                  aaaTCTTCCTGATGCAATATTTCTACTGGTAATAAGGACTGTAATTTCATGAAAATAAAAACTATTTTAACGCCAGTAACCTGCGCTCTGCTGATAAGTTTTTCCGCCCATGCCGCTAACGCCGACAA‑TTATAAAAACGTGATTAACCGTACTGACGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGa                                                                                                                                                    <  1:122506/251‑1 (MQ=255)                                                                                               gCAATACTTCTACTGGTAATAAGGCCTGTAATTTCATGAAAATAAAAACTATTGTAACGCCAGTAACCTTCTCTCTGCTGATAAGTTTTTCCGCCCATGCCGCTAACGCCGACAA‑TTATAAAAACTTGATTAACCGTTCTGTCGCGCCGCAGTACATGAATGATTACGATTACGTCGATCACCAGCGTTTTAAGCCGTTTTTCGATCTCGGAGCCTGACATGGTCATCTGTTGCCAGACGGCTCTAacac                                                                                                                                        <  2:21883/250‑1 (MQ=255)                                                                                                       tCTACTGGTAATAAGGACTGTAATTTCATGAAAATAAAAACTATTTTAACGCCAGTAACCTGCGCTCTGCTGATAAGTTTTTCCGCCCATGCCGCTAACGCCGACAA‑TTATAAAAACGTGATTAACCGTACTGACGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGggcg                                                                                                                                <  2:285018/250‑1 (MQ=255)                                                                                                                               ttCATGAAAATAAAAACTATTTTAACGCCAGTAACCTGCGCTCTGCTGATAAGTTTTTCCGCCCATGCCGCTAACGCCGACAA‑TTATAAAAACGTGATTAACCGTACTGACGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGcc                                                                                                                                                       >  2:174649/1‑203 (MQ=255)                                                                                                                               ttCATGAAAATAAAAACTATTTTAACGCCAGTAACCTGCGCTCTGCTGATAAGTTTTTCCGCCCATGCCGCTAACGCCGACAA‑TTATAAAAACGTGATTAACCGTACTGACGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGcc                                                                                                                                                       <  1:174649/203‑1 (MQ=255)                                                                                                                                tCATGAAAATAAAAACTATTTTAACGCCAGTAACCTGCGCTCTGCTGATAAGTTTTTCCGCCCATGCCGCTAACGCCGACAA‑TTATAAACACGTGATTAACCGTACTGACGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACg                                                                                                      >  2:109385/1‑251 (MQ=255)                                                                                                                                       aaTAAAAACTATTTTAACGCCAGTAACCTGCGCTCTGCTGAGAAGTTTTTCCGCCCATGCCGCTAACGCCGACAA‑TTATAAAAACGTGATTAACCGTACTGACGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTGCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGt                                                                                               <  2:12913/251‑1 (MQ=255)                                                                                                                                        aTAAAAACTATTTTAACGCCAGTAACCTGCGCTCTGCTGATAAGTTTTTCCGCCCATGCCGCTAACGCCGACAA‑TTATAAAAACGTGATTAACCGTACTGACGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTGTTCGATCGCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGTa                                                                                              <  1:120853/251‑1 (MQ=255)                                                                                                                                             aaCTATTTTAACGCCAGTAACCTGCGCTCTGCTGATAAGTTTTTCCGCCCATGCCGCTAACGCCGACAA‑TTATAAAAACGTGATTAACCGTACTGACGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGTACATc                                                                                          >  2:172678/1‑250 (MQ=255)                                                                                                                                                 aTTTTAACGCCAGTAACCTGCGCTCTGCTGATAAGTTTTTCCGCCCATGCCGCTAACGCCGACAA‑TTATAAAAACGTGATTAACCGTACTGACGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGTACATCAACtt                                                                                     >  1:97585/1‑251 (MQ=255)                                                                                                                                                    ttAACGCCAGTAACCTGCGCTCTGCTGATAAGTTTTTCCGCCCATGCCGCTAACGCCGACAA‑TTATAAAAACGTGATTAACCGTACTGACGCGCCGCAGTACCTGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGTACATCAACTTTAt                                                                                  >  1:161377/1‑251 (MQ=255)                                                                                                                                                             gTAACCTGCGCTCTGCTGATAAGTTTTTCCGCCCATGCCGCTAACGCCGACAA‑TTATAAAAACGTGATTAACCGTACTGACGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGTACATCAACTTTATGGCCAGCaa                                                                         <  1:172678/251‑1 (MQ=255)                                                                                                                                                                    gcgcTCTGCTGATAAGTTTTTCCGCCCATGCCGCTAACGCCGACAA‑TTATAAAAACGTGATTAACCGTACTGACGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGTACATCAACTTTATGGCCAGCAATTTCg                                                                    <  1:109385/249‑1 (MQ=255)                                                                                                                                                                                   gTTTTTCAGCCCTGGCCGCTAAAGCC‑ACAAATTATAAAAACGTGTTTAACCTCACCGGCCCGCCGCAGTACATGAAGGATTACGATTACGCCGACCGCCAACGTTTTAATCCGTTTTGCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACTCCATGCGCGTCTTTCCGGGCGTTGCGCTGCTCACGGAAGAGTACATCAACTTTACGGCCTGCGATTTCGCCCGCCTGACCGTCTgt                                                   <  2:191911/251‑2 (MQ=255)                                                                                                                                                                                         ccGCCCATGCCGCTAACGCCGACAA‑TTATAAAAACGTGATTAACCGTACTGACGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGTACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGAc                                             <  1:18248/251‑1 (MQ=255)                                                                                                                                                                                             ccATGCCGCTAACGCCGACAA‑TTATAAAAACGTGATTAACCGTACTGACGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGTACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCa                                         <  1:178543/251‑1 (MQ=255)                                                                                                                                                                                             ccATGCCGCTAACGCCGACAA‑TTATAAAAACGTGATTAACCGTACTGACGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCCTTCTCGGAGCCTGGCATGGTCATCTGTTGCAAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGTACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGc                                          >  1:152273/1‑250 (MQ=255)                                                                                                                                                                                                 gCCGCTAACGCCGACAA‑TTATAAAAACGTGATTAACCGTACTGACGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGGGTACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGa                                      >  1:250364/1‑250 (MQ=255)                                                                                                                                                                                                   cGCTAACGCCGACAA‑TTATAAAAACGTGATTAACCGTACTGACGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGTACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAg                                   <  1:81427/251‑1 (MQ=255)                                                                                                                                                                                                          gCCGACAA‑TTATAAAAACGTGATTAACCGTACTGACGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGTACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACtt                            <  1:102646/251‑1 (MQ=255)                                                                                                                                                                                                             gACAA‑TTATAAAAACGTGATTAACCGTACTGACGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGTACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACTTTAc                         <  2:238517/251‑1 (MQ=255)                                                                                                                                                                                                             gACAA‑ATATAAAAACGTGATTAACCGTACTGACGCGCCGCAGTTCATGTAGGATTCCGGTTACGACGATCCCCAACGTTTTAATCCGTTTTTCGACCCCGGAGCCTGGCATGGTCATCGGTTGCCAGACGGCCATAACCCCATGGGCGTCTGTCCGGGCGTTGCGCTGGTGACGGAAGAGTACATCAACTTTATGACCAGCAAGTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACtttgc                         <  2:68757/251‑3 (MQ=255)                                                                                                                                                                                                                   ttATAAAAACGTGATTAACCGTACTGACGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGTACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACTTTACGCTgg                    >  1:58891/1‑251 (MQ=255)                                                                                                                                                                                                                          aaCGTGATTAACCGTACTGACGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGTACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACTTTACGCTGGAGGCAt              >  1:173183/1‑250 (MQ=255)                                                                                                                                                                                                                                     ccGTACTGACGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGTACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACTTTACGCTGGAGGCATACAGTATTCCCg  >  1:230692/1‑251 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                   ACGAATACGGCGGCGGACGTTTCGCCACGGCTTTTGATAGCCGCTTCTACATGAAAAATGCCTCTCACGATCCCAGCGACCAAATCTTCCTGATGCAATATTTCTACTGGTAATAAGGACTGTAATTTCATGAAAATAAAAACTATTTTAACGCCAGTAACCTGCGCTCTGCTGATAAGTTTTTCCGCCCATGCCGCTAACGCCGACAA‑TTATAAAAACGTGATTAACCGTACTGGCGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGATCTCGGAGCCTGGCATGGTCATCTGTTGCCAGACGGCCCTAACACCATGGGCGGCTTTCCGGGCGTTGCGCTGCTGACGGAAGAGTACATCAACTTTATGGCCAGCAATTTCGACCGCCTGACCGTCTGGCAGGACGGCAAGAAAGTCGACTTTACGCTGGAGGCATACAGTATTCCCG  >  NC_000913/3228759‑3229236

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑