breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 3,645 G→A W282* (TGG→TAG)  thrB → homoserine kinase
RA 71,170 T→C S262P (TCG→CCG)  araC → DNA‑binding transcriptional dual regulator AraC
RA 81,044 C→T L305L (CTG→CTA leuB ← 3‑isopropylmalate dehydrogenase
RA 81,102 G→A S286L (TCG→TTG)  leuB ← 3‑isopropylmalate dehydrogenase
RA 85,203 C→T P279L (CCG→CTG)  leuO → DNA‑binding transcriptional dual regulator LeuO
RA 154,182 C→T L415L (CTG→CTA htrE ← putative fimbrial usher protein HtrE
RA 156,068 A→G V45A (GTA→GCA)  yadV ← putative fimbrial chaperone YadV
JC JC 168,803 IS1 (+) +9 bp coding (1320‑1328/2244 nt) fhuA → ferrichrome outer membrane transporter/phage receptor
RA 223,560 T→A intergenic (+152/‑211) gmhB → / → rrsH D‑glycero‑beta‑D‑manno‑heptose‑1,7‑bisphosphate 7‑phosphatase/16S ribosomal RNA
RA 246,985 C→T P92S (CCG→TCG)  yafL → NlpC/P60 family protein YafL
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 284,535 T→G A445A (GCT→GCG yagF → D‑xylonate dehydratase
RA 292,019 C→T L174L (CTA→TTA)  yagJ → protein YagJ
RA 309,381 C→T D216N (GAT→AAT)  ecpB ← putative fimbrial chaperone EcpB
RA 309,985 T→A G14G (GGA→GGT ecpB ← putative fimbrial chaperone EcpB
JC JC 362,204 IS1 (–) +9 bp coding (968‑976/1254 nt) lacY ← lactose permease
RA 410,666 C→T P175S (CCT→TCT)  mak → fructokinase
RA 421,027 C→T R14R (CGC→CGT proY → putative transporter ProY
RA 456,711 C→G P12R (CCC→CGC)  clpP → ATP‑dependent Clp protease proteolytic subunit
RA 472,849 A→G G84G (GGA→GGG glnK → nitrogen regulatory protein PII‑2
RA 495,622 C→T T168I (ACC→ATC)  htpG → chaperone protein HtpG
RA 534,742 G→A G276D (GGT→GAT)  gcl → glyoxylate carboligase
RA 547,891 C→T L404L (CTC→CTT fdrA → putative oxamate carbamoyltransferase FdrA
MC JC 575,786 Δ3,398 bp IS5‑mediated [nmpC]borD [nmpC], essD, rrrD, rzpD, rzoD, borD
RA 582,597 C→T intergenic (+500/‑278) tfaD → / → tfaX putative tail fiber assembly protein TfaD/protein TfaX
RA 694,941 G→A L5L (CTC→CTT miaB ← isopentenyl‑adenosine A37 tRNA methylthiolase
RA 696,470 C→T noncoding (35/75 nt) glnX ← tRNA‑Gln
RA 780,624 C→T noncoding (71/76 nt) lysT → tRNA‑Lys
RA 789,580 C→A E134* (GAA→TAA)  galK ← galactokinase
RA 790,832 Δ1 bp coding (198/1047 nt) galT ← galactose‑1‑phosphate uridylyltransferase
RA 822,527 C→T E405K (GAA→AAA)  clsB ← cardiolipin synthase B
RA 883,396 C→T intergenic (‑8/‑277) ybjG ← / → mdfA undecaprenyl pyrophosphate phosphatase/multidrug efflux pump MdfA/Na(+):H(+) antiporter/K(+):H(+) antiporter
RA 884,892 C→T S407F (TCT→TTT)  mdfA → multidrug efflux pump MdfA/Na(+):H(+) antiporter/K(+):H(+) antiporter
RA 905,283 G→T G124V (GGC→GTC)  amiD → N‑acetylmuramoyl‑L‑alanine amidase D
RA 908,169 C→T R38R (AGG→AGA ybjT ← putative NAD(P)‑binding protein YbjT
RA 909,990 C→T E354K (GAG→AAG)  poxB ← pyruvate oxidase
RA 922,954 G→T A14A (GCG→GCT clpS → specificity factor for ClpA‑ClpP chaperone‑protease complex
RA 1,085,420 T→A S143R (AGT→AGA phoH → ATP‑binding protein PhoH
JC JC 1,085,983 IS1 (+) +8 bp coding (992‑999/1065 nt) phoH → ATP‑binding protein PhoH
RA 1,123,823 2 bp→AG coding (231‑232/648 nt) grxB ← reduced glutaredoxin 2
RA 1,147,792 T→A S58T (TCG→ACG)  plsX → putative phosphate acyltransferase
RA 1,169,836 A→G L180P (CTG→CCG)  ldtC ← L,D‑transpeptidase LdtC
RA 1,189,980 A→G T156T (ACT→ACC phoP ← DNA‑binding transcriptional dual regulator PhoP
RA 1,194,922 G→A T10T (ACC→ACT rluE ← 23S rRNA pseudouridine(2457) synthase
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
RA 1,253,002 C→T intergenic (+17/+83) dhaR → / ← ycgV DNA‑binding transcriptional dual regulator DhaR/putative autotransporter adhesin YcgV
MC JC 1,299,499 Δ1,199 bp insH21 insH21
RA 1,301,992 A→T N271Y (AAT→TAT)  oppA → oligopeptide ABC transporter periplasmic binding protein
RA 1,306,736 T→G S325A (TCC→GCC)  oppF → murein tripeptide ABC transporter/oligopeptide ABC transporter ATP binding subunit OppF
RA 1,308,684 T→C intergenic (‑39/‑104) clsA ← / → yciY cardiolipin synthase A/uncharacterized protein YciY
RA 1,331,059 2 bp→AT coding (12‑13/2598 nt) topA → DNA topoisomerase 1
RA 1,337,394 A→G S522G (AGC→GGC)  acnA → aconitate hydratase 1
RA 1,358,859 T→C Y110C (TAT→TGT)  puuP ← putrescine:H(+) symporter PuuP
RA 1,405,758 G→A intergenic (‑7/‑221) mcaS ← / → smrA small regulatory RNA McaS/DNA endonuclease SmrA
RA 1,428,150 G→A L193L (CTG→TTG)  insH5 ← IS5 family transposase and trans‑activator
RA 1,489,491 G→A L420L (CTG→CTA aldA → aldehyde dehydrogenase A
RA 1,497,385 4 bp→CAA coding (530‑533/1656 nt) opgD → glucans biosynthesis protein D
RA 1,629,247 C→T I11I (ATC→ATT ydfZ → putative selenoprotein YdfZ
JC JC 1,641,158 IS1 (–) +8 bp intergenic (+183/+174) ynfU → / ← cspB putative zinc‑binding protein YnfU/cold shock‑like protein CspB
RA 1,643,679 A→T L209Q (CTG→CAG)  ydfU ← protein YdfU
RA 1,652,331 T→C intergenic (+2346/+397) ydfD → / ← ynfP lysis protein/protein YnfP
RA 1,667,460 G→A Q369* (CAG→TAG)  mlc ← DNA‑binding transcriptional repressor Mlc
RA 1,748,841 C→T R240K (AGA→AAA)  ydhT ← uncharacterized protein YdhT
RA 1,751,024 C→A intergenic (‑11/+53) ydhX ← / ← ydhW putative 4Fe‑4S ferredoxin‑like protein YdhX/uncharacterized protein YdhW
RA 1,848,300 2 bp→TT coding (376‑377/552 nt) ydjA ← putative oxidoreductase YdjA
JC JC 1,879,829 Δ1 bp :: IS186 (+) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
RA 1,894,839 T→C L12P (CTC→CCC)  pabB → aminodeoxychorismate synthase subunit 1
RA 1,962,172 C→T A67A (GCC→GCT yecT → protein YecT
MC JC 1,978,503 Δ776 bp insB5insA5 insB5, insA5
RA 2,002,087 C→T noncoding (20/195 nt) fliX ← small RNA FliX
RA 2,003,219 T→G T130P (ACC→CCC)  fliC ← flagellar filament structural protein
RA 2,008,395 C→T A40V (GCT→GTT)  yedE → putative selenium transporter YedE
RA 2,010,068 C→T T83I (ACC→ATC)  yedK → genome maintenance protein
RA 2,023,559 A→G T223A (ACT→GCT)  fliR → flagellar biosynthesis protein FliR
RA 2,027,332 T→A E229V (GAA→GTA)  dgcQ ← diguanylate cyclase DgcQ
RA 2,029,550 C→T R4R (CGC→CGT yedA → putative transporter YedA
RA 2,031,160 C→T E386E (GAG→GAA dcm ← DNA‑cytosine methyltransferase
RA 2,032,183 C→T W45* (TGG→TGA dcm ← DNA‑cytosine methyltransferase
RA 2,033,648 C→T intergenic (‑227/‑1) drpB ← / → rseX cell division protein DrpB/small regulatory RNA RseX
RA 2,040,433 C→A A319D (GCC→GAC)  msrP → protein‑L‑methionine sulfoxide reductase catalytic subunit MsrP
RA 2,041,278 G→A intergenic (+160/‑97) msrQ → / → zinT membrane‑bound flavocytochrome MsrQ/metal‑binding protein ZinT
RA 2,046,403 C→T A489V (GCT→GTT)  yeeJ → inverse autotransporter adhesin
RA 2,052,885 G→A intergenic (+871/‑758) yeeJ → / → shiA inverse autotransporter adhesin/shikimate:H(+) symporter
JC JC 2,094,553 IS1 (+) +9 bp coding (19‑27/591 nt) hisH → imidazole glycerol phosphate synthase subunit HisH
RA 2,104,024 G→T I162I (ATC→ATA wbbK ← putative glycosyltransferase WbbK
RA 2,111,259 Δ1 bp coding (718/900 nt) rfbD ← dTDP‑4‑dehydrorhamnose reductase
RA 2,142,598 G→A L117L (CTC→CTT udk ← uridine/cytidine kinase
JC JC 2,168,341 IS5 (–) +4 bp intergenic (‑35/‑368) yegR ← / → yegS uncharacterized protein YegR/lipid kinase YegS
MC JC 2,171,429 Δ5,958 bp IS3‑mediated [gatR]gatY [gatR], gatD, gatB, gatA, gatZ, gatY
RA 2,233,609 C→T Q4* (CAG→TAG)  yeiS → DUF2542 domain‑containing protein YeiS
RA 2,242,247 C→T G241R (GGG→AGG)  yeiB ← DUF418 domain‑containing protein YeiB
RA 2,247,134 A→C Y467D (TAC→GAC)  lysP ← lysine:H(+) symporter
RA 2,281,855 T→C G408G (GGT→GGC radD → putative DNA repair helicase RadD
RA 2,281,954 C→A N441K (AAC→AAA radD → putative DNA repair helicase RadD
RA 2,404,402 G→A S71L (TCG→TTG)  nuoB ← NADH:quinone oxidoreductase subunit B
RA 2,556,984 G→A T105I (ACC→ATC)  eutC ← ethanolamine ammonia‑lyase subunit beta
RA 2,587,112 C→T A461V (GCA→GTA)  narQ → sensor histidine kinase NarQ
RA 2,609,473 G→A L329L (CTG→CTA hyfG → hydrogenase 4 catalytic subunit HyfG
RA 2,774,947 G→A G544S (GGT→AGT)  yfjW → uncharacterized protein YfjW
RA 2,867,455 G→A Q33* (CAG→TAG)  rpoS ← RNA polymerase sigma factor RpoS
RA 2,880,635 T→C L138L (TTA→TTG casD ← type I‑E CRISPR system Cascade subunit CasD
RA 2,926,772 C→A G155G (GGC→GGA ppnN → nucleotide 5'‑monophosphate nucleosidase
RA 3,025,601 (T)5→4 coding (1251/1401 nt) xanQ → xanthine:H(+) symporter XanQ
RA 3,070,858 T→G K129N (AAA→AAC fbaA ← fructose‑bisphosphate aldolase class II
RA 3,087,508 C→T P268L (CCA→CTA)  metK → methionine adenosyltransferase
RA 3,167,148 G→A H191Y (CAT→TAT)  ygiS ← putative deoxycholate binding periplasmic protein
RA 3,207,549 Δ1 bp coding (179/606 nt) ttdB → L(+)‑tartrate dehydratase subunit beta
RA 3,279,276 C→G F121L (TTC→TTG kbaZ → putative tagatose‑1,6‑bisphosphate aldolase 1 chaperone
RA 3,296,481 G→A V25M (GTG→ATG)  dolP → division and outer membrane stress‑associated lipid‑binding lipoprotein
RA 3,329,743 G→T D261Y (GAT→TAT)  dacB → peptidoglycan DD‑endopeptidase DacB
RA 3,388,041 T→G T50P (ACG→CCG)  aaeB ← aromatic carboxylic acid efflux pump subunit AaeB
RA 3,411,689 G→T E13* (GAA→TAA)  yhdJ → DNA adenine methyltransferase
RA 3,433,900 C→T L71L (CTA→TTA)  def → peptide deformylase
RA 3,465,502 G→A E141K (GAG→AAG)  gspM → Type II secretion system protein GspM
RA 3,474,424 T→G K43N (AAA→AAC rpsL ← 30S ribosomal subunit protein S12
RA 3,486,502 C→T T128I (ACT→ATT)  crp → DNA‑binding transcriptional dual regulator CRP
RA 3,511,466 C→T V293V (GTG→GTA yhfZ ← putative DNA‑binding transcriptional regulator YhfZ
RA 3,524,913 C→T F681F (TTC→TTT mrcA → peptidoglycan glycosyltransferase/peptidoglycan DD‑transpeptidase MrcA
RA 3,555,047 2 bp→TG coding (1963‑1964/2706 nt) malT → DNA‑binding transcriptional activator MalT
RA 3,560,455 +G intergenic (+589/+167) rtcR → / ← glpG DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
RA 3,581,222 A→G N29D (AAC→GAC)  yhhY → N‑acetyltransferase YhhY
RA 3,647,428 C→T A377V (GCG→GTG)  gor → glutathione reductase (NADPH)
RA 3,685,480 C→T E47K (GAA→AAA)  pdeK ← c‑di‑GMP phosphodiesterase PdeK
RA 3,707,947 C→A intergenic (‑242/+669) dppA ← / ← proK dipeptide ABC transporter periplasmic binding protein/tRNA‑Pro
RA 3,719,429 G→A intergenic (‑385/‑49) yiaF ← / → yiaG DUF3053 domain‑containing protein YiaF/putative DNA‑binding transcriptional regulator YiaG
RA 3,725,176 T→G E48A (GAG→GCG)  glyQ ← glycine‑‑tRNA ligase subunit alpha
RA 3,729,440 2 bp→TT noncoding (105‑106/160 nt) xylZ ← small RNA XylZ
RA 3,730,560 C→T W69* (TGG→TAG)  xylA ← xylose isomerase
RA 3,770,539 C→T V281M (GTG→ATG)  yibH ← inner membrane protein YibH
RA 3,773,173 Δ1 bp coding (893/1914 nt) mtlA → mannitol‑specific PTS enzyme IICBA component
RA 3,773,176 C→T S299F (TCT→TTT)  mtlA → mannitol‑specific PTS enzyme IICBA component
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,926,991 G→A L5L (CTG→TTG)  asnC ← DNA‑binding transcriptional dual regulator AsnC
RA 3,966,530 2 bp→TT coding (114‑115/1260 nt) rho → transcription termination factor Rho
JC JC 4,044,847 IS5 (+) +4 bp coding (649‑652/1431 nt) yihF → DUF945 domain‑containing protein YihF
JC JC 4,124,494 IS2 (+) +5 bp intergenic (‑38/+114) cytR ← / ← priA DNA‑binding transcriptional repressor CytR/primosomal protein N'
RA 4,223,638 T→C intergenic (‑10/‑190) iclR ← / → metH DNA‑binding transcriptional repressor IclR/cobalamin‑dependent methionine synthase
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,322,288 A→G F45F (TTT→TTC phnF ← putative transcriptional regulator PhnF
RA 4,325,000 C→T G56S (GGC→AGC)  phnC ← phosphonate/phosphate ABC transporter ATP binding subunit
RA 4,325,644 C→T S33N (AGC→AAC)  yjdN ← PF06983 family protein YjdN
RA 4,331,890 C→T L463L (CTC→CTT proP → osmolyte:H(+) symporter ProP
RA 4,437,890 C→T Q62* (CAG→TAG)  ytfI → protein YtfI
RA 4,473,900 C→T G52R (GGA→AGA)  bdcA ← c‑di‑GMP‑binding biofilm dispersal mediator protein
RA 4,475,493 T→C F19F (TTT→TTC yjgL → protein YjgL
RA 4,476,605 G→A S390N (AGC→AAC)  yjgL → protein YjgL
RA 4,489,016 C→T E350K (GAA→AAA)  yjgR ← DUF853 domain‑containing protein YjgR
RA 4,510,238 T→G intergenic (+445/+452) insO → / ← fecE IS911B regulator fragment/ferric citrate ABC transporter ATP binding subunit

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1196364 1211475 15112 2 [1] [1] 2 [icd]–mcrA [icd],C0293,ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,ymfN,ymfR,beeE,ymfQ,ycfK,tfaP,tfaE,pinE,mcrA
* * ÷ NC_000913 1579803 1579852 50 2 [1] [1] 2 ydeM putative anaerobic sulfatase maturation enzyme YdeM
* * ÷ NC_000913 2558699 2565482 6784 42 [1] [1] 5 intZ–[eutA] intZ,yffL,yffM,yffN,yffO,yffP,yffQ,yffR,yffS,[eutA]
* * ÷ NC_000913 3423900–3424231 3424424–3424238 8–525 3 [1] [1] 2 rrlD 23S ribosomal RNA
* * ÷ NC_000913 4035410–4035728 4036863–4035735 8–1454 2 [1] [1] 2 [rrsA] [rrsA]
* * ÷ NC_000913 4542050 4548165 6116 20 [0] [0] 2 [fimE]–[fimF] [fimE],fimA,fimI,fimC,fimD,[fimF]
* * ÷ NC_000913 4548357 4576732 28376 2 [0] [0] 45 [fimG]–yjiT [fimG],fimH,gntP,uxuA,uxuB,uxuR,yjiC,ytiC,ytiD,idlP,iraD,hypT,iadA,yjiG,yjiH,kptA,yjiJ,yjiK,ytiA,yjiL,yjiM,yjiN,mdtM,ytiE,yjiR,yjiS,yjiT

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 156143 =8 (0.250)3 (0.090) 3/488 2.4 27.3% coding (59/741 nt) yadV putative fimbrial chaperone YadV
?NC_000913 273955 = NA (NA)noncoding (1195/1195 nt) IS5 repeat region
* ? NC_000913 = 1561469 (0.280)16 (0.490) 16/488 0.7 64.0% coding (56/741 nt) yadV putative fimbrial chaperone YadV
?NC_000913 = 275149 NA (NA)noncoding (1/1195 nt) IS5 repeat region
* ? NC_000913 257908 =NA (NA)12 (0.370) 12/488 1.0 50.0% noncoding (768/768 nt) IS1 repeat region
?NC_000913 431322 = 12 (0.370)coding (692/885 nt) tsx nucleoside‑specific channel‑forming protein Tsx
* ? NC_000913 273955 =NA (NA)8 (0.250) 8/486 1.4 38.1% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 538670 = 13 (0.400)coding (1038/1455 nt) ybbW putative allantoin transporter
* ? NC_000913 = 382398NA (NA)5 (0.160) 5/484 1.9 NA noncoding (1139/1331 nt) IS2 repeat region
?NC_000913 = 382590 NA (NA)noncoding (1331/1331 nt) IS2 repeat region
* ? NC_000913 = 43133011 (0.340)17 (0.530) 17/488 0.7 60.7% coding (684/885 nt) tsx nucleoside‑specific channel‑forming protein Tsx
?NC_000913 = 1979270 NA (NA)noncoding (1/768 nt) IS1 repeat region
* ? NC_000913 = 53867314 (0.430)9 (0.280) 9/488 1.3 39.1% coding (1041/1455 nt) ybbW putative allantoin transporter
?NC_000913 = 575785 NA (NA)noncoding (1/1195 nt) IS5 repeat region
* ? NC_000913 607765 =1 (0.030)32 (0.990) 28/486 0.2 97.0% noncoding (28/60 nt) sokE small RNA SokE
?NC_000913 = 3722075 NA (NA)noncoding (1443/1443 nt) IS150 repeat region
* ? NC_000913 = 118654110 (0.310)7 (0.220) 7/486 1.6 41.2% coding (698/1227 nt) pepT peptidase T
?NC_000913 = 1469240 NA (NA)noncoding (1/1331 nt) IS2 repeat region
* ? NC_000913 = 139420812 (0.380)4 (0.130) 4/482 2.1 25.0% coding (982/1614 nt) mppA murein tripeptide ABC transporter periplasmic binding protein
?NC_000913 1396044 = NA (NA)noncoding (1/1195 nt) IS5 repeat region
* ? NC_000913 = 13945486 (0.190)9 (0.280) 9/488 1.3 60.0% coding (1322/1614 nt) mppA murein tripeptide ABC transporter periplasmic binding protein
?NC_000913 = 2290113 NA (NA)noncoding (1/1195 nt) IS5 repeat region
* ? NC_000913 1446828 =11 (0.340)7 (0.220) 7/488 1.6 38.9% coding (456/906 nt) feaR DNA‑binding transcriptional activator FeaR
?NC_000913 1467910 = NA (NA)noncoding (1331/1331 nt) IS2 repeat region
* ? NC_000913 1743497 =9 (0.280)9 (0.280)
+G
8/486 1.4 50.1% coding (41/1374 nt) mdtK multidrug efflux pump MdtK
?NC_000913 2101749 = NA (NA)noncoding (1195/1195 nt) IS5 repeat region
* ? NC_000913 1978503 =NA (NA)44 (1.360) 38/488 0.1 100% noncoding (768/768 nt) IS1 repeat region
?NC_000913 4576733 = 0 (0.000)intergenic (+2802/+179) yjiT/mcrC putative uncharacterized protein YjiT/Type IV methyl‑directed restriction enzyme EcoKMcrBC subunit
* ? NC_000913 = 25586981 (0.030)41 (1.310) 36/472 0.1 98.8% intergenic (‑19/‑160) eutB/intZ ethanolamine ammonia‑lyase subunit alpha/putative phage integrase IntZ
?NC_000913 2565489 = 0 (0.000)coding (1396/1404 nt) eutA ethanolamine ammonia‑lyase reactivase EutA
* ? NC_000913 3583428 =NA (NA)20 (0.620) 16/486 0.7 100% noncoding (1/768 nt) IS1 repeat region
?NC_000913 = 4542049 0 (0.000)coding (13/597 nt) fimE regulator for fimA
* ? NC_000913 4092855 =17 (0.530)5 (0.160) 5/486 1.9 22.7% intergenic (‑32/+269) frvA/rhaM putative PTS enzyme IIA component FrvA/L‑rhamnose mutarotase
?NC_000913 4498181 = NA (NA)noncoding (1/1331 nt) IS2 repeat region