Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,010,068	C→T	T83I (ACC→ATC)	yedK →	genome maintenance protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,010,068	0	C	T	100.0%	36.5 / NA	14	T83I (ACC→ATC)	yedK	genome maintenance protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/8);  total (6/8)

ACGCTTTGCCCAATCCCAAACGCGTGAAGATTACCTTG‑CGCTTCTCGCGGAAGATATTGAACGCGATATTCCCTATGATCCCGAACCCATTGGCAGATACAACGTCGC‑GCCGGGAACCAAAGTCCTACTGCTCAGTGAACGTGATGAACACCTTCATCTGGATCCGGTTTT‑CTGGGGATATGCTCCCGGATGGTGGGATAAACCGCCGCTGATTAACGCCCGCGTAGAAACTGCGGCAACCAGTCGTATGTTTAAACCGCTCTGGCAACATGGTCGGGCAATCTGTTTTGCCGATGGCTGGTTTGAGTGGAAAAAAGAAGGCGACAAAAAACAGCCTTTTTTTATCTATCGTGCTGATGGACAACCTATTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACGAAGCCGAAGGATTTTTGATCGTCACTGCTGCGGCAGATCAAGG  >  NC_000913/2009829‑2010284                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                         aCGCTTTGCCCAATCCCAAACGCGTGAAGATTACCTTG‑CGCTTCTCGCGGAAGATATTGAACGCGATATTCCCTATGATCCCGAACCCATTGGCAGATACAACGTCGC‑GCCGGGAACCAAAGTCCTACTGCTCAGTGAACGTGATGAACACCTTCATCTGGATCCGGTTTT‑CTGGGGATATGCTCCCGGATGGTGGGATACACCGCCGCTGATTAACGCCAGCGTAGAAACTGCGGCAATCAGTCGTATGt                                                                                                                                                                                                               >  2:117648/1‑251 (MQ=255)   gCTTTGCCCAATCCCAAACGCGTGAAGATTGCCTTG‑CGCTTCTCGCGGAAGATATTGAACGCGATATTCCCTATGATCCCGACCCCATTGGCAGATACAACGTCGC‑GCCGGGAACCAAAGTCCTACTGCTCAGTGAACGTGATGAACACCTTCATCTGGATCCGGTTTT‑CTGGGGAAATGCTCCCGGATGGTGGGATAAACCGCCGCTGATTAACGCCCGCGTAGAAACTGCGGCAATCAGTCGGATGttt                                                                                                                                                                                                             <  1:242983/251‑1 (MQ=255)     tttGCCCAATCCCAAACGCGTGAAGATTACCTTG‑CGCTTCTCGCGGAAGATATTGAACGCGATATTCCCTATGATCCCGAACCCATTGGCAGATACAACGTCGC‑GCCGGGAACCAAAGTCCTACTGCTCAGTGAACGTGATGAACCCCTTCATCTGGATCCGGTTTT‑CTGGGGATATGCTCCCGGATGGTGGGATAAACCGCCGCTGATTAACGCCCGCGTAGAAACTGCGGCAATCAGTCGTATGttt                                                                                                                                                                                                             <  1:239882/249‑1 (MQ=255)        ccccAATCCCAAACGCTTGAAGATT‑CCTTGGCGCTTCTCGCGGAAGATATTGACCGCGATATTCCCTAGGATCCCGAACCCATTGGCAGATACAACGTCGC‑GCCGGGACCCAAAGGCCTACTGCTCAGTGAACGTGAGGAACACCTGCATCTGGATCCGTTTTG‑CTGGGGATATTCTCCCGGATGTTGGGATAAACCGCCGCTGATTAATGCCCGCGTAGAAACTGCGGCAATCAGTCGTATGTTTAAAcc                                                                                                                                                                                                        <  1:106524/250‑1 (MQ=255)                        gTGAAGATTACCTTG‑CGCTTCTCGCGGAAGATATTGAACGCGATATTCCCTATGATCCCGAACCCATTGGCAGATACAACGTCGC‑GCCGGGAACCAAAGTCCTACTGCTCAGTGAACGTGATGAACACCTTCATCTGGATCCGGTTTT‑CTGGGGATATGCTCCCGGATGGTGGGATAAACCGCCGCTGATTAACGCCCGCGTAGAAACTGCGGCAATCAGTCGTATGTTTAAACCGCTCTGGCAACATGGt                                                                                                                                                                                        >  1:163372/1‑251 (MQ=255)                                         gcTTCTCGCGTAAGAAATTGACCGCGATATTCCCTATAAACCCGACCCCTTTGGCAGATACAACG‑CGCCGCCGGGAACCAAAGTCCTACTGCTCAGTTAACGTGAGGATCATCGTCATGTGTA‑CCGGTTTTTCGGGGGATATGCTCCCGGGTGGTGGTATAAACCGCCGCTGATTAACGCCCGCGTAGAAATTGCGGCAATCAGTCGTATGTTTAAACCGCTCTGCCAACATGGTCGGGGAATCTGTTTTg                                                                                                                                                                        <  2:163372/251‑1 (MQ=255)                                            tctcGCGGAAGATATTGAACGCGATATTCCCTATGATCCCGAACCCATTGGCAGATACAACGTCGC‑GCCGGGAACCAAAGTCCTACTGCTCAGTGAACGTGATGAACACCTTCATCTGGATCCGGTTTT‑CTGGGGATATGCTCCCGGATGGTGGGATAAACCGCCGCTGATTAACGCCCGCGTAGAAACTGCGGCAATCAGTCGTATGTTTAAACCGCTCTGGCAACATGGTCGGGCAATCTGTTTTGCCg                                                                                                                                                                     >  1:276764/1‑251 (MQ=255)                                                gaggAAGATATTGAACGCGATATTCCCTATGATCCCGAACCCATTGGCAGATACAACGTCGA‑GCCGGGAACCAAAGTCCTACTGCTCCGTGAACGTGATGAACACCTTCATCTGGATACGGTTTT‑CTGGGGATATGCTCCCGGATGGTGGGATAAACCGCCGCTGATTAACGCCCGCGTAGAAACTGCGGCAATCAGTCGTAGGTTTAAACCGCTCTGGCAACATGGTCGGGCTATATGTTTTGCCGATgg                                                                                                                                                                 >  2:260376/3‑251 (MQ=255)                                                                                     accccATGGGCATATACAACGTCGC‑GCGGGGAACCAAAGTCCTACTGCTCAGTGAACGTGATGAACCCTTTCATCTGGATCCGTTTTT‑CTGGGGTTATGCTCCCGGATGGTGGGATAAACCGCCGCTGATTAACGCCCGCGTAGAAACTGCGGCAATCAGTCGTATGTTTAAACCGCTCTGGCAACATGGTCGGGCAATCTGTTTTGCCGATGGCTGGTTTGAGTGGAAAAAAGAAGGCGACAAAAAACAg                                                                                                                            <  2:184307/249‑1 (MQ=255)                                                                                                                 cGGGAACCAAAGTCCTACTGCTCAGTGAACGTGATGAACACCTTCATCTGGATCCGGTTTT‑CTGGGGATATGCTCCCGGATGGTGGGATAAACCGCCGCTGATTAACGCCCGCGTAGAAACTGCGGCAATCAGTCGTATGTTTAAACCGCTCTGGCAACATGGTCGGGCAATCTGTTTTGCCGATGGCTGGTTTGAGTGGAAAAAAGAAGGCGACaaa                                                                                                                                  >  2:229820/1‑218 (MQ=255)                                                                                                                 cGGGAACCAAAGTCCTACTGCTCAGTGAACGGGATGAACCCCTTCATCTGGATCCGGTTTT‑CTGGGGATATGCTCCCTGATGGTGGGATAAACCGCCGCTGATTAACGCCCGCGTAGAAACTGCGGCAATCAGTCGTATGTTTAAACCGCTCTGGCAACATGGTCGGGCAATCTGTTTTGCCGATGGCTGGTTTGAGTGGAAAAAAGAAGGCGACaaa                                                                                                                                  <  1:229820/218‑1 (MQ=255)                                                                                                                              ccttcTGCTCAGTGACCGTGATAACCCCCTTCATCTGGAACCGGTTTT‑CGGGGGATATGGGCCCGGATGGGGGGATAAACCGCCGCTGATTAACGCCCGCGTAGAAACTGCGGCAATCAGTCGTATGTTTAAACCGCTCTGGCAACATGGTCGGGCAATCTGTTTTGCCGATGGCTGGTTTGAGTGGAAAAAAGAAGGCGACAAAAAACAGCCTTTTTTTATCTATCGTGCTGATGGACAACCTAttttt                                                                                     <  2:76104/246‑1 (MQ=255)                                                                                                                                                                                                   ggtggGATAAACCGCCGCGGATGAACGCCCGCGTAGAAACTGCGGCAATCAGTCGTATGGTGAAACCGCGCGGGGAACATGGTCGGACAAACTGTTTTGCCGATGGCTGGGTTGAGTGGAAAAAAGAAGGCAACAAAAAACATCCTTTATGTAACTATCTTGATGATGGACAACCTATTTTTATGGCCGCGATAGGTAGCACACCAATTGAGCGTGGTTACGAAGCCGAAGGACTTTTGATCGTCActgct                <  1:168974/251‑1 (MQ=255)                                                                                                                                                                                                                 ccgcTGATTAACGCCCGCGTAGAAACTGCGGCAATCAGTCGTATGTTTAAACCGCTCTGGCAACATGGTCGGGCAATCTGTTTTGCCGATGGCTGGTTTGAGTGGAAAAAAGAAGGCGACAAAAAACAGCCTTTTTTTATCTATCGTGCTGATGGACAACCTATTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACGAAGCCGAAGGATTTTTGATAGTCACTGCTGCGGCAGATCAAgg  >  1:13223/1‑251 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                         ACGCTTTGCCCAATCCCAAACGCGTGAAGATTACCTTG‑CGCTTCTCGCGGAAGATATTGAACGCGATATTCCCTATGATCCCGAACCCATTGGCAGATACAACGTCGC‑GCCGGGAACCAAAGTCCTACTGCTCAGTGAACGTGATGAACACCTTCATCTGGATCCGGTTTT‑CTGGGGATATGCTCCCGGATGGTGGGATAAACCGCCGCTGATTAACGCCCGCGTAGAAACTGCGGCAACCAGTCGTATGTTTAAACCGCTCTGGCAACATGGTCGGGCAATCTGTTTTGCCGATGGCTGGTTTGAGTGGAAAAAAGAAGGCGACAAAAAACAGCCTTTTTTTATCTATCGTGCTGATGGACAACCTATTTTTATGGCCGCGATAGGTAGCACACCATTTGAGCGTGGTGACGAAGCCGAAGGATTTTTGATCGTCACTGCTGCGGCAGATCAAGG  >  NC_000913/2009829‑2010284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑