Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	908,169	C→T	R38R (AGG→AGA)	ybjT ←	putative NAD(P)‑binding protein YbjT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	908,169	0	C	T	100.0%	103.7 / NA	32	R38R (AGG→AGA)	ybjT	putative NAD(P)‑binding protein YbjT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/12);  total (20/12)

GCTCATGTGGCGGGGCCTGCAACGAACTAAGAAAGATTAATTGCTTAACTGGTACTTCACGTAGCGCATCGCGGACGTTGAGAGCCACCTGGCGCTCCTGAGCGATAAAATCGCCGCCTTCGCCCATGCTGTGCACCAGAAAATAGACCGTATCGATATCCTGCAACAGGGCCGGTAGGTTATCCGGCCAGCTGAGATCGACTTTATGGCAACTGACGTTTGCCAGTTGCAGCTTTGCAAGCCTGTCGACATGACGTGCCGCCGCCAGGATCTGATGCCCTTGCTGGCTGAGTGTGCGCACCAGATGCTGACCAATGTAGCCACTGGCACCGAGAACTAAAATGCGTTGCGGCACGTCTCTCTCCTTAACGCGCCAGGAATGCACGCCAGTGGGCGGCGACTTCCGCCAGTTGTTCGCGCGAGACGTCAAGATGCGTCACCAGGCGGACAATCGGCGAGGCGTTAATCAGCACGTTTCTCGCTTTCAT  >  NC_000913/907928‑908415                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                       gCTCATGTGGCGGGGCCTGCAACGAACTAAGAAAGATTAATTGCTTAACTGGTACTTCACGTAGCGCATCGCGGACGTTGAGAGCCACCTGGCGCTCCTGAGCGATAAAATCGCCGCCTTCGCCCATGCTGTGCACCAGCAAATAGACCGGATCGATATCCTGCAACAGGGCCGGTAGGTTATCCGGCCAGCTGAGATCGACTTTATGGCAACTGACGATTGACAGTTGCAGCTTTGCAAGTCTGTCGAcc                                                                                                                                                                                                                                               >  1:39970/1‑250 (MQ=255)    cATGTGGCGGGGCCTGCAAAGAACTAAGAAAGATTAATTGCTTAACTGGGACTTCACGTAGCGCATCGCGGACGGTGAGAGCCACCTGGCGCTCCTGAGCGGTAAAATCGCCGCCTTCGCCCATGCTGTGCACCAGAAAATAGACCGTATCGATATCCTGCAACAGGGCCGGGAGGGTATCCGGCCCGCTGAGATCGGCTTTATGGCAACTGACGTTTGCCCGTTGCAGCTTTGCAAGTCTGTCGACATg                                                                                                                                                                                                                                             >  1:70055/1‑250 (MQ=255)     aTGTGGCGGGGCCTGCAACGAACTAAGAAAGATTAATTGCTTAACTGGTACTTCACGTAGCGCATCGCGGACGTTGAGAGCCACCTGGCGCTCCTGAGCGATAAAAGCGCCGCCTTCGCCCATGCTGTGCACCAGAAAATAGACCGTATCGATATCCTGCAACAGGGCCGGTAGGTTATCCGGCCAGCTGAGATCGACTTTATGGCAACTGACGTTTGCCAGTTGCAGCTTTGCAAGTCTGTCGACATGAc                                                                                                                                                                                                                                           <  1:315328/251‑1 (MQ=255)          gCGGAGCCTGCAACGAACTAAGAAAGATTAATTGCTTAACTGGTACTTCACGTAGCGCATCGCGGACGTTGAGAGCCACCTGGCGCTCCTGAGCGATAAAATCGCCGCCTTCGCCCATGCTGTGCACCAGAAAATAGACCGTATCGATATCCTGCAACAGGGCCGGTAGGTTATCCGGCCAGCTGAGATCGACTTTATGGCAACTGACGTTTGCCAGTTGCAGCTTTGCAAGTCTGTCGACATGACGTgcc                                                                                                                                                                                                                                      <  1:77428/251‑1 (MQ=255)                             aagaaagaTTAATTGCTTAACTGGTACTTCACGTAGCGCATCGCGGACGTTGAGAGCCACCTGGCGCTCCTGAGCGATAAAATCGCCGCCTTCGCCCATGCTGTGCACCAGAAAATAGACCGTATCGATATCCTGCAACAGGGCCGGTAGGTTATCCGGCCAGCTGAGATCGACTTTATGGCAACTGACGTTTGCCAGTTGCAGCTTTGCAAGTCTGTCGACATGACGTGCCGCCGCCAGGATCTGATg                                                                                                                                                                                                                     <  1:236022/249‑1 (MQ=255)                                          tGCTTAACTGGTACTTCACGAAGCGCATCGCGGCCGTTGAGAGCCACCTGGCGCTCCTCAGCGATAAAATCGCCGCCTTCGCCCATGCTGTGCACCAGAAAATAGACCGTATCGATATCCTGCAACAGGGCCGGTAGGTTATCCGGCCAGCTGAGATCGACTTTATAGCAACTGACGTTTGCCAGTTTCAGCTTTGCAAGTCTGTCGACATGACGTGCCGCCGCCCGGAGCTGATGCCCTTgctggctg                                                                                                                                                                                                        >  2:62470/1‑249 (MQ=255)                                                 cTGGTACTTCACGTAGCGCATCGCGGACGTTGAGAGCCACCTGGCGATCCTGAGCGATAAAATCGCCGCCTTCGCCCATGCTGTGCACCAGAAAATAGACCGTATCGATATCCTGCAACAGGGCCGGTAGGTTATCCGGCCAGCTGAGATCGACTTTATGGCAACTGACGTTTGCCAGTTGCAGCTTTGCAAGTCTGTCGACATGACGTGCCGCCGCCAGGATCTGATGCCCTTGCTGGCTGAGTGTgcgc                                                                                                                                                                                               >  1:214629/1‑251 (MQ=255)                                                        ttCACGTAGCGCATCGCGGACGTTGAGAGCCACCTGGCGCTCCTGAGCGATAAAATCGCCGCCTTCGCCCATGCTGTGCACCAGAAAATAGACCGTATCGATATCCTGCAACAGGGCCGGTAGGTTATCCGGCCAGCTGAGATCGACTTTATGGCAACTGACGTTTGCCAGTTGCAGCTTTGCAAGTCTGTCGACATGACGTGCCGCCGCCAGGATCTGATGCCCTTGCTGGCTGAGTGTGCGCAcc                                                                                                                                                                                            >  2:247573/1‑247 (MQ=255)                                                          cACGTAGCGCATCGCGGACGTTGATAGCCACCTGTCGCTCCTGAGCGATAAAATCGCCGCCTTCGCCCATGCTGTGCACCAGAAAATAGACCGTATCGATATCCTGCAACAGGGCCGGTAGGTTATCCGGCCAGCTGAGATCGACTTTATGGCAACTGACGTTTGCCAGTTGCAGCTTTGCAAGTCTGGCGACAGGACGTGCCGCCGCCAGGATCTGAGGCCCTTGCTTGCTGAGTGTGCGCACCAGATGc                                                                                                                                                                                      <  2:202936/251‑1 (MQ=255)                                                          cACGTAGCGCAGCACGGCTGTTGCGAGCCACATCGCGCTCCTGCGCGATCACATCGCCGCCTCAGCCCATGCTCTGCTCCAGAAACTAGACCGAATCGATACCATGCCACCGGGCCGGTAGGTTATCCGGCCAGCTGAGATCGACTTTATGGCAACTGCCGTTTGCCCGTTGCCGCTTTGCACGTCTGTCGACATGACGTGCCGCCGCCAGGATCTGCTGCACTTGCTGGCTGAGTGTGCGCACCAGATGc                                                                                                                                                                                      >  2:49411/1‑251 (MQ=255)                                                            cGTAGCGCATCGCGGACGTTGAGAGCCACCTGGCGCTCCTGAGCGATAAAATCGCCGCCTTCGCCCATGCTGTGCACCAGAAAATAGACAGTATCGATATCCTGCAACAGGGCCGGTAGGTTATCCGGCCAGCTGAGATCGACTTTATGGCAACTGACGTTTGCCAGTTGCAGCTTTGCAAGTCTGTCGCCATGACGTGCCGCCGCCAGGATCTGATGCCCTTGCTGGCTGAGTGTGCGCACCAGATGCTg                                                                                                                                                                                    >  2:189167/1‑251 (MQ=255)                                                                       gcgGACGTTGAGAGCCACCTGGCGCTCCTGAGCGATAAAATCGCCGCCTTCGCCCATGCTGTGCACCAGAAAATAGACCGTATCGATATCCTGCAACAGGGCCGGTAGGTTATCCGGCCAGCTGAGATCGACTTTATGGCAACTGACGTTTGCCAGTTGCAGCTTGGCAAGTCGGTCGACATGACGTGCCGCCGCCAGGATCTGATGCCCTTGCTGGCTGAGTGTGCGCACCAGATGCTGACCAATGTAGc                                                                                                                                                                         <  1:317139/251‑1 (MQ=255)                                                                        cgGACGTTGAGAGCCACCTGGCGCTCCTGAGCGATAAAATCGCCGCCTTCGCCCATGCTGTGCACCAGAAAATAGACCGTATCGATATCCTGCAACAGGGCCGGTAGGTTATCCGGCCAGCTGAGATCGACTTTATGGCAACTGACGTTTGCCAGTTGCAGCTTTGCAAGTCTGTCGACATGACGTGCCGCCGCCAGGATCTGATGCCCTTGCTGGCTGAGTGTGCGCACCAGATGCTGACCAATGTAGcc                                                                                                                                                                        >  1:176004/1‑251 (MQ=255)                                                                                 agagCCACCTGGCGCTCCTGAGCGATAAAATCGCCGCCTTCGCCCATGCTGTGCACCAGAAAATAGACCGTATCGATATCCTGCAACAGGGCCGGTAGGTTATCCGGCCAGCTGAGATCGACTTTATGGCAACTGACGTTTGCCAGTTGCAGCTTTGCAAGTCTGTCGACATGACGTGCCGCCGCCAGGATCTGATGCCCTTGCTGGCTGAGTGTGCGCACCAGATGCTGACCAATGTAGCCACTGGCAcc                                                                                                                                                               <  1:146552/251‑1 (MQ=255)                                                                                           ggCGCTCCTGAGCGATAAAATCGCCGCCTTCGCCCATGCTGTGCACCAGAAAATAGACCGTATCGATATCCTGCAACAGGGCCGGTAGGTTATCCGGCCAGCTGAGATCGACTTTATGGCAACTGACGTTTGCCAGTTGCAGCTTTGCAAGTCTGTCGACATGACGTGCCGCCGCCAGGATCTGATGCCCTTGCTGGCTGAGTGTGCGCACCAGATGCTGACCAATGAAGCCACTGGCACCGGGAACTaa                                                                                                                                                      >  2:100386/1‑250 (MQ=255)                                                                                             cgcTCCTGAGCGATAAAATCGCCGCCTTCGCCCATGCTGTGCACCAGAAAATAGACCGTATCGATATCCTGCAACAGGGCCGGTAGGTTATCCGGCCAGCTGAGATCGACTTTATGGCAACTGACGTTTGCCAGTTGCAGCTTTGCAAGTCTGTCGACATGACGTGCCGCCGCCAGGATCTGATGCCCTTGCTGGCTGAGTGTGCGCACCAGATGCTGACCAATGTAGCCACAGGCACCGAGACCTAAAAt                                                                                                                                                   >  1:286920/1‑251 (MQ=255)                                                                                                   tGAGCGATAAAATCGCCGCCTTCGCCCATGCTGTGCACCAGAAAATAGACCGTATCGATATCCTGCAACAGGGCCGGTAGGTTATCCGGCCAGCTGAGATCGACTTTATGGCAACTGACGTTTGCCAGTTGCAGCTTTGCAAGTCTGTCGACATGACGTGCCGCCGCCAGGATCTGATGCCCTTGCTGGCTGAGGGTGCGCACCAGATGCTGACCAATGTAGCCACTGGCACCGAGAACTAAAATGCGTTg                                                                                                                                             >  2:145309/1‑251 (MQ=255)                                                                                                                 gccgccTTCGCCCATGCTGTGCACCAGAAAATAGACCGTATCGATATCCTGCAACAGGGCCGGTAGGTTATCCGGCCAGCTGAGATCGACTTTATGGCAACTGACGTTTGCCAGTTGCAGCTTTGCAAGTCTGTCGACATGACGTGCCGCCGCCAGGATCTGATGCCCTTGCTGGCTGAGTGTGCGCACCAGATGCTGACCAATGTAGCCACTGGCACCGAGAACTAAAATGCGTTGCGGCACGtctctct                                                                                                                               <  1:247573/251‑1 (MQ=255)                                                                                                                           cccATGCTGTGCACCAGAAAATAGAACGTATCGATATCCTGCAACAGGGCCGGTAGGTTATCCGGCCAGCTGAGATCGACTTTATGGCAACTGACGTTTGCCAGTTGCAGCTTTGCAAGTCTGTCGACATGACGTGCCGCCGCCAGGATCTGATGCCCTTGCTGGCTGAGTGTGCGCACCAGCTGCTGACCAATGTAGCCCCTGGCACCGAGAACTAAAATGCGTTGCGGCACGTCTCTCTCCTTAAcgcc                                                                                                                     >  2:194856/1‑250 (MQ=255)                                                                                                                                                 aGACCGTATCGATATCCTGCAACAGGGCCGGTAGGTTATCCGGCCAGCTGAGATCGACTTTATGGCAACTGACGTTTGCCAGTTGCAGCTTTGCAAGTCTGTCGACATGACGTGCCGCCGCCAGGATCTGATGCCCTTGCTGGCTGAGTGTGCGCACCAGATGCTGACCAATGTAGCCACTGGCACCGAGAACTAAAATGCGTTGCGGCACGTCTCTCTCCTTAACGCGCCAGGAATGCACGCCAGTGggc                                                                                               >  2:323715/1‑251 (MQ=255)                                                                                                                                                  aaCCGTATCGATATCCTGCAACAGGGCCTGTAGTTTATCCGGTCACCTGAGATCGACTTTATGGCAACTGACTTTTTCCAGTTGCAGCTTTGCAAGTCTGCCGACATGATGTGCCGCCGCCAGGTTCTGATGCCCTTGCTGGCTGAGTGTGCGCACCAGATGCTGACCAAGGTAGCCACTGGCACCGAGAACTAAAATGCGTTTCGACACTTCTCTCTCCGTATCGCGCCAGGAATGCACGCCAGTGggcg                                                                                              <  1:186172/250‑1 (MQ=255)                                                                                                                                                                           gCCGGTAGGTTATCCGGCCAGCTGAGATCGACTTTATGGCAACTGACGTTTGCCAGTTGCAGCTTTGCAAGTCTGTCGACATGACGTGCCGCCGCCAGGATCTGATGCCCTTGCTGGCTGAGTGTGCGCACCAGATGCTGACCAATGTAGCCACTGGCACCGAGAACTAAAATGCGTTGCGGCACGTCTCTCTCCTTAACGCGCCAGGAATGCACGCCAGTGGGCGGCGACTTCCGCCAGTTGTTcgcgcg                                                                     >  1:236645/1‑251 (MQ=255)                                                                                                                                                                                                  aggaTCAACTGTATGGCAACTGACGTGTGCCAGTTGCCGCTTTGCAAGTCTGTCGACATGACGTGCCGCCGCCAGGATCTGATGCCCTTGCTGGCTGAGTGTGCGCACCAGATGCTGACCACTGTAGCCACTGGCACCGAGAACTAAAATGCGTTGCGGCACGTCTCTCCCCTTAACGCGCCAGGAATGCACGCCAGTGGGCGGCGACTTCCGCCAGTTGTTCGCGCGAGACGTCAAGATGCGTCACCa                                                <  2:4464/247‑1 (MQ=255)                                                                                                                                                                                                   agaTCGACTTTATGGCAACTGACGTTTGCCAGTTGCAGCTTTGCAAGTCGGTCGACATGACGTGCCGCCGCCAGGATCTGATGCCCTTGCTGGCTGAGTGTGCGCACCAGATGCTGACCAATGTAGCCACTGGCACCGAGAACTAAAATGCGTTGCGGCACGTCTCTCTCCTTAACGCGCCAGGAATGCACGCCAGTGGGCGGCGACTTCCGCCAGTTGTTCGCGCGAGACGTCAAGATGCGTCACCAGGc                                             <  2:305683/251‑1 (MQ=255)                                                                                                                                                                                                     aTCGACTTTATGGCAACTGCCGTTTGCCAGTTGCAGCTTTGCAAGTCTGTCGACATGCCGTGCCGCCGCCAGGATCTGATGCCCTTGCTGGCTGAGTGTGCGCACCAGATGCTGACCAATGTAGCCACTGGCACCGAGAACTAAAATGCGTTGCGGCACGTCTCTCTCCTTAACGCGCCAGGAATGCACGCCAGTGGGCGGCGACTTCCGCCAGTTGTTCGCGCGAGACGTCAAGATGCGTCACCAGGCgg                                           <  2:176004/251‑1 (MQ=255)                                                                                                                                                                                                         aCTTTATGGCAACTGACGTTTGCCAGTTGCAGCTTTGCAAGTCTGTCGACATGACGTGCCGCCGCCAGGATCTGATGCCCTTGCTGGCTGAGTGTGCGCACCAGATGCTGACCAATGTAGCCACTGGCACCGAGAACTAAAATGCGTTGCGGCACGTCTCTCTCCTTAACGCGCCAGGAATGCACGCCAGTGGGCGGCGACTTCCGCCAGTTGTTCGCGCGCGACGTCAAGATGCGTCACCAGGCGGACa                                        >  2:75704/1‑250 (MQ=255)                                                                                                                                                                                                              aTGGCAACTGACGTTTGCCAGTTGCAGCTTTGCAAGTCTGTCGACATGACGTGCCGCCGCCAGGATCTGATGCCCTTGCTGGCTGAGTGTGCGCACCAGATGCTGACCAATGTAGCCACTGGCACCGAGAACTAAAATGCGTTGCGGCACGTCTCTCTCCTTAACGCGCCAGGAATGCACGCCAGTGGGCGGCGACTTCCGCCAGTTGTTCGCGCGAGACGTCAAGATGCGTCACCAGGCGGACAATCggg                                  >  1:150329/1‑250 (MQ=255)                                                                                                                                                                                                                   aaCTGACGTTTGCCAGTTGCAGCTTTGCAAGTCTGTCGACATGACGTGCCGCCGCCAGGATCTGATGCCCTTGCTGGCTGAGTGTGCGCACCAGATGCTGACCAATGTAGCCACTGGCACCGAGAACTAAAATGCGTTGCGGCACGTCTCTCTCCTTAACGCGCCAGGAATGCACGCCAGTGGGCGGCGACTTCCGCCAGTTGTTCGCGCGAGACGTCAAGATGCGTCACCAGGCGGACAATCGGCGAGGc                             >  1:305027/1‑251 (MQ=255)                                                                                                                                                                                                                         cGTTTGCCAGTTGCAGCTTTGCAAGTCTGTCGACATGACGTGCCGCCGCCAGGATCTGATGCCCTTGCTGGCTGAGTGTGCGCACCAGATGCTGACCAATGTAGCCACTGGCACCGAGAACTAAAATGCGTGGCGGCACGTCTCTCTCCTTAACGCGCCAGGAATGCACGCCAGTGGGCGGCGACTTCCGCCAGTTGTTCGCGCGAGACGTCAAGATGCGTCACCAGGCGGACAATCGGCGAGGCGTTAAt                       <  2:70055/251‑1 (MQ=255)                                                                                                                                                                                                                              gCCAGTTGCAGCTTTGCAAGTCTGTCGACATGACGTGCCGCCGCCAGGATCTGATGCCCTTGCTGGCTGAGTGTGCGCACCAGATGCTGACCAATGTAGCCACTGGCACCGAGAACTAAAATGCGTTGCGGCACGTCTCTCTCCTTAACGCGCCAGGAATGCACGCCAGTGGGCGGCGACTTCCGCCAGTTGGTCGCGCGATACGTCAAGATGCGTCACCAGGCGGACACTCGGCGCGGCGTTAATCAGcc                  >  2:513/1‑250 (MQ=255)                                                                                                                                                                                                                                     gCAGCTTTGCAAGTCTGTCGACATGACGTGCCGCCGCCAGGATCTGATGCCCTTGCTGGCTGAGTGTGCGCACCAGATGCTGACCAATGTAGCCACTGGCACCGAGAACTAAAATGCGTTGCGGCACGTCTCTCTCCTTAACGCGCCAGGAATGCACGCCAGTGGGCGGCGACTTCCGCCAGTGGTTCGCGCGAGACGTCAAGATGCGTCACCAGGCGGACAATCGGCGCGGCGTTAATCAGCACGTTtct           >  2:82842/1‑251 (MQ=255)                                                                                                                                                                                                                                              cAAGTCTGTCGACATGACGTGCCGCCGCCAGGATCTGATGCCCTTGCTGGCTGAGTGTGCGCCCCAGATGCTGACCAATGTAGCCACTGGCACCGAGAACTAAAATGCGTTGCGGCACGTCTCTCTCCTTAACGCGCCAGGAATGCACGCCAGTGGGCGGCGACTTCCGCCAGTTGTTCGCGCGAGACGTCAAGATGCGTCACCAGGCGGACAATCGGCGAGGCGTTAATCAGCACGTTTCTCGCTTTCAt  >  2:287213/1‑251 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                       GCTCATGTGGCGGGGCCTGCAACGAACTAAGAAAGATTAATTGCTTAACTGGTACTTCACGTAGCGCATCGCGGACGTTGAGAGCCACCTGGCGCTCCTGAGCGATAAAATCGCCGCCTTCGCCCATGCTGTGCACCAGAAAATAGACCGTATCGATATCCTGCAACAGGGCCGGTAGGTTATCCGGCCAGCTGAGATCGACTTTATGGCAACTGACGTTTGCCAGTTGCAGCTTTGCAAGCCTGTCGACATGACGTGCCGCCGCCAGGATCTGATGCCCTTGCTGGCTGAGTGTGCGCACCAGATGCTGACCAATGTAGCCACTGGCACCGAGAACTAAAATGCGTTGCGGCACGTCTCTCTCCTTAACGCGCCAGGAATGCACGCCAGTGGGCGGCGACTTCCGCCAGTTGTTCGCGCGAGACGTCAAGATGCGTCACCAGGCGGACAATCGGCGAGGCGTTAATCAGCACGTTTCTCGCTTTCAT  >  NC_000913/907928‑908415

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑