Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,002,087	C→T	noncoding (20/195 nt)	fliX ←	small RNA FliX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,002,087	0	C	T	100.0%	84.4 / NA	25	noncoding (20/195 nt)	fliX	small RNA FliX
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/12);  total (13/12)

TGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGCAATTTGGCGTTGCCGTCAGTCTCAGTTAATCAGGTTACAACGATTAACCCTGCAGCAGAGACAGAACCTGCTGCGGTACCTGGTTAGCTTTTGCCAACACGGAGTTACCGGCCTGCTGGATGATCTGCGCTTTCGACATATTGGACACTTCGGTCGCATAGTCGGCGTCCTGAATACGGGACTGCGCTTCAGACAGGTTGGTAGTGGTGTTGTTCAGGTTGGTAACCGCGGAATCCAGACGGTTTTGCACCGCACCGAGGGAA  >  NC_000913/2001868‑2002329                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                   tGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGCAATTTGGCGTTGCTGTCAGTTTCAGTTAATCAGGTTACAACGATTAACCCTg                                                                                                                                                                                                                     >  1:257513/1‑251 (MQ=255)         ggggATATTTGGGGGATACAGGTAAATTCCAGGCAGACAAAAACCACGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCACCTTGTAGGCCTGATAAGCGCAGCGCATCAGGCAATTTGGCGTTGCCGTAAGTTTCAGTTAATCAGGTTACAACGATTAACCCTGcagcag                                                                                                                                                                                                               >  2:231998/1‑249 (MQ=38)                 ttGGGGGTTACAGGTAAATTCCAGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCAGCAGGCAATTTGGCGTTGCCGTCAGTTTCAGTTAATCAGGTTACAACGATTAACCCTGCAGCAGAGACAGAAc                                                                                                                                                                                                      <  1:34293/250‑1 (MQ=255)                             ggTAAATTCCAGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGCAATTTGGCGTTGCCGTCAGTTTCAGTTAATCAGGTTACAACGATTAACCCTGCAGCATAGACAGAACCTGCTGCGGTa                                                                                                                                                                                           <  2:193868/249‑1 (MQ=255)                                 aaTTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGCAATTTGGCGTTGCCGTCAGTTTCAGTTAATCAGGTTACAACGATTAACCCTGCAGCAGAGACAGAACCTGCTGCGGTACCTGGt                                                                                                                                                                                     >  1:176311/1‑251 (MQ=255)                                                           ggTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGCAATTTGGCGTTGCCGTCAGTTTCAGTTAATCAGGTTACAACGATTAACCCTGCAGCAGAGACAGAACCTGCTGCGGTACCTGGTTAGCTTTTGCCAACACGGAGTTACCg                                                                                                                                                           <  1:225781/251‑1 (MQ=255)                                                                cGGGGAAGCACTTGGCTGACAAAGTGCGACTTATGTGTCCGGATGCGGAGTAAACTCCTTATCCTGCCTACAAAAATGTGCAAATTCAATAAATTGCAATGCAACTGGGAGTCCTTATAAGCGCAGCGCATCAGGCAATTTGGCGTTGCCGTCAGTTTCAGTTAAGCAGGTTACAACGAGTAACCCTGCAGCAGAGACAGAACCTGCTGCGGTACCTGTTTAGCTTTTGTCAAAACGGAGTTACCGGCctt                                                                                                                                                      <  2:185514/251‑2 (MQ=255)                                                                        cACGTTGCTGACAAATTGCGCTTTAAGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGCAATTTGGCGTGGCCGTCAGTTTCAGTTAATCAGGTTACAACGATTAACCCTGCAGCAGAGACAGAACCTGCTGCGGTACCTGGTTAGCTTTTGCCAACACGGAGTTACCGGCCTGCTGgatga                                                                                                                                              <  1:135839/251‑1 (MQ=255)                                                                                                aTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCACTAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGCAATTTGGCGTTGCCGTCAGTTTCAGTTAATCAGGTTACAACGATTAACCCTGCAGCAGAGACAGAACCTGCTGCGGTACCTGGTTAGCTTTTGCCAACACGGAGTTACCGGCCTGCTGGATGATCTGCGCTTTCGACATATTGGac                                                                                                                       >  2:151873/1‑250 (MQ=255)                                                                                                             cggcgTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGCAATTTGGCGTTGCCGTCAGTTTCAGTTAATCAGGTTACAACGATTAACCCTGCAGCAGAGACAGAACCTGCTGCGGTACCTGGTTAGCTTTTGCCAACACGGAGTTACCGGCCTGCTGGATGATCTGCGCTTTCGACATATTGGACACTTCGGTCGCAt                                                                                                          >  1:237447/1‑250 (MQ=255)                                                                                                                cgTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGCAATTTGGCGTTGCCGTCAGTTTCAGTTAATCAGGTTACAACGATTAACCCTGCAGCAGAGACAGAACCTGCTGCGGTACCTGGTTAGCTTTTGCCAACACGGAGTTACCGGCCTGCTGGATGATCTGCGCTTTCGACATATTGGACACTTCGGTCGCATAGTc                                                                                                      >  1:219926/1‑251 (MQ=255)                                                                                                                              ccGGCCTACAAAAAGGTGCAAAGTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGCAATTTGGCGTTGCCGTCAGTTTCAGTTAATCAGGTTACAACGATTAACCCTGCAGCAGAGACAGAACCTGCTGCGGTACCTGGTTAGCGTTTGCCAACACGGAGGTACCGGCCTGGTGGATGATCTGCGCTTTCGACAGAGTGGACACTTCGGTCGCATGGTTGGCGTCCTGAATa                                                                                         <  2:109284/250‑1 (MQ=255)                                                                                                                                  ccTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGCAATTTGGCGTTGCCGTCAGTTTCAGTTAATCAGGTTACAACGATTAACCCTGCAGCAGAGACAGAACCTGCTGCGGTACCTGGTTAGCTTTTGCCAACACGGAGTTACCGGCCTGCTGGATGATCTGCGCTTTCGACATATTGGACACTTCGGTCGCATAGTCGGCGTCCTGAATACGGGa                                                                                    <  1:10315/251‑1 (MQ=255)                                                                                                                                             gtgCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGCAATTTGGCGTTGCCGTCAGTTTCAGTTAATCAGGTTACAACGATTAACCCTGCAGCAGAGACAGAACCTGCTGCGGTACCTGGTTAGCTTTTGCCAACACGGAGTTACCGGCCTGCTGGATGATCTGCGCTTTCGACATATTGGACACTTCGGTCGCATAGTCGGCGTCCTGAATACGGGACTGCGCTTc                                                                           >  1:305332/1‑249 (MQ=255)                                                                                                                                               gCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGCAATTTGGCGTTGCCGTCAGTTTCAGTTAATCAGGTTACAACGATTAACCCTGCAGCAGAGACAGAACCTGCTGCGGTACCTGGTTAGCTTTTGCCAACACGGAGTTACCGGCCTGCTGGATGATCTGCGCTTTCGACATATTGGACACTTCGGTCGCATAGTCGGCGTCCTGAATACGGGACTGCGCTTCAGAc                                                                       <  1:161522/251‑1 (MQ=255)                                                                                                                                                              tGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGCAATTTGGCGTTGCCGTCAGTTTCAGTTAATCAGGTTACAACGATTAACCCTGCAGCAGAGACAGAACCTGCTGCGGTACCTGGTTAGCTTTTGCCAACACGGAGTTACCGGCCTGCTGGATGATCTGCGCTTTCGACATATTGGACACTTCGGTCGCATAGTCGGCGTCCTGAATACGGGACTGCGCTTCAGACAGGTTGGTAgtggtg                                                        >  1:255066/1‑251 (MQ=255)                                                                                                                                                                            tAGGCCTGATAAGCGCCGCGCATCAGGCAATTTGGCGTTGCCGTCAGTTTCAGTTAATCAGGTTACAACGATTAACCCTGCAGCAGAGACAGAACCTGCTGCGGTACCTGGTTAGCTTTTGCCAac                                                                                                                                                                       >  1:270152/1‑126 (MQ=255)                                                                                                                                                                            tAGGCCTGATAAGCGCAGCGCATCAGGCAATTTGGCGTTGCCGTCAGTTTCAGTTAATCAGGTTACAACGATTAACCCTGCAGCAGAGACAGAACCTGCTGCGGTACCTGGTTAGCTTTTGCCAac                                                                                                                                                                       <  2:270152/126‑1 (MQ=255)                                                                                                                                                                                 cTGATAAGCGCAGCGCATCAGGCAATTTGGCGTTGCCGTCAGTTTCAGTTAATCAGGTTACAACGATTAACCCTGCAGCAGAGACAGAACCTGCTGCGGTACCTGGTTAGCTTTTGCCAACACGGAGTTACCGGCCTGCTGGATGATCTGCGCTTTCGACATATTGGACACt                                                                                                                    >  1:276594/1‑172 (MQ=255)                                                                                                                                                                                 cTGATAAGCGCAGCGCATCAGGCAATTTGGCGTTGCCGTCAGTTTCAGTTAATCAGGTTACAACGATTAACCCTGCAGCAGAGACAGAACCTGCTGCGGTACCTGGTTAGCTTTTGCCAACACGGAGTTACCGGCCTGCTGGATGATCTGCGCTTTCGACATATTGGACACt                                                                                                                    <  2:276594/172‑1 (MQ=255)                                                                                                                                                                                                         aTTTGGCGCTGCCGTCAGTTTCAGTTAATCAGGTTACAACGATTAACCCTGCAGCAGAGACAGAACCTGCTGCGGTACCTGGTTAGCTTTTGCCAACACGGAGTTACCGGCCTGCTGGATGATCTGCGCTTTCGACATATTGGACACTTCGGTCGCATAGTCGGCGTCCTGAATACGGGACTGCGCTTCAGACAGGTTGGTAGTGGTGTTGTTCAGGTTGGTAACCGCGGAATCCAGACGGTTTTgcaccg             >  1:247588/1‑251 (MQ=255)                                                                                                                                                                                                              gCGTTGCCGTCAGTTTCAGTTAATCAGGTTACAACGATTAACCCTGCAGCAGAGACAGAACCTGCTGCGGTACCTGGTTAGCTTTTGCCAACACGGAGTTACCGGCCTGCTGGATGATCTGCGCTTTCGACATATTGGACACTTCGGTCGCATAGTCGGCGTCCTGAATACGGGACTGCGCTTCAGTCAGGTTGGTAGTGGTGTTGTTCAGGTTGGTAGCCGCGGAATCCAGACGGTTTTGcaccgcaccg        <  2:206377/251‑1 (MQ=255)                                                                                                                                                                                                                   gctgTCAGTTTCAGTTAATCAGGTTACAACGATTAACCCTGCAGCAGAGACAGAACCTGCTGCGGTACCTGGTTAGCTTTTGCCAACACGGAGTTACCGGCCTGCTGGATGATCTGCGCTTTCGACATATTGGACACTTCGGTCGCATAGTCGGCGTCCTGAATACGGGACTGCGCTTCAGACAGGTTGGTAGTGGTGTTGTTCAGGTTGGTAACCGCGGAATCCAGACGGTTTTGCACCGCACCGAggg    >  1:10378/4‑250 (MQ=255)                                                                                                                                                                                                                   gCCGTCAGTTTCAGTTAATCAGGTTACAACGATTAACCCTGCAGCAGAGACAGAACCTGCTGCGGTACCTGGTTAGCTTTTGCCAACACGGAGTTACCGGCCTGCTGGATGATCTGCGCTTTCGACATATTGGACACTTCGGTCGCATAGTCGGCGTCCTGAATACGGGACTGCGCTTCAGACAGGTTGGTAGTGGTGTTGTTCAGGTTGGTAACCGCGGAATCCAGACGGTTTTGCACCGCACCGAggg    >  1:10368/1‑250 (MQ=255)                                                                                                                                                                                                                    ccGTCAGTTTCAGTTAATCAGGTTACAACGATTAACCCTGCAGCAGAGACAGAACCTGCTGCGGTACCTGGTTAGCTTTTGCCAACACGGAGTTACCGGCCTGCTGGATGATCTGCGCTTTCGACATATTGGACACTTCGGTCGCATAGTCGGCGTCCTGAATACGGGACTGCGCTTCAGACAGGTTGGTAGTGGTGTTGTTCAGGTTGGTAACCGCGGAATCCAGACGGTTTTGCACCGCACCGAGGgaa  <  2:305332/251‑1 (MQ=255)                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                   TGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGCAATTTGGCGTTGCCGTCAGTCTCAGTTAATCAGGTTACAACGATTAACCCTGCAGCAGAGACAGAACCTGCTGCGGTACCTGGTTAGCTTTTGCCAACACGGAGTTACCGGCCTGCTGGATGATCTGCGCTTTCGACATATTGGACACTTCGGTCGCATAGTCGGCGTCCTGAATACGGGACTGCGCTTCAGACAGGTTGGTAGTGGTGTTGTTCAGGTTGGTAACCGCGGAATCCAGACGGTTTTGCACCGCACCGAGGGAA  >  NC_000913/2001868‑2002329

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑