Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,087,508	C→T	P268L (CCA→CTA)	metK →	methionine adenosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,087,508	0	C	T	100.0%	146.0 / NA	44	P268L (CCA→CTA)	metK	methionine adenosyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/16);  total (28/16)

TCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACGTTATGTCGCGAAAAACATCGTTGCTGCTGGCCTGGCCGATCGTTGTGAAATTCAGGTTTCCTACGCAATCGGCGTGGCTGAACCGACCTCCATCATGGTAGAAACTTTCGGTACTGAGAAAGTGCCTTCTGAACAACTGACCCTGCTGGTACGTGAGTTCTTCGACCTGCGCCCATACGGTCTGATTCAGATGCTGGATC  >  NC_000913/3087264‑3087744                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                             tCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCTATCaa                                                                                                                                                                                                                                         >  1:102288/1‑250 (MQ=255)               tGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCTATCAAAAGAGGACCGTTCCg                                                                                                                                                                                                                          >  1:286100/1‑251 (MQ=255)                      aTCGACCAGAAATCGCTGCAAGAAGCGGTAATGGACGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCCTTCTCTGGTAAAGATCTATCAAAAGTGGACCGTTCCGCAGCCt                                                                                                                                                                                                                    >  2:238055/1‑250 (MQ=255)                         gACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCTATCAAAAGTGGACCGTTCCGCAGCCTACgc                                                                                                                                                                                                                >  1:119880/1‑251 (MQ=255)                          aCCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCTATCAAAAGTGGACCGTTCCGCAGCCTACgc                                                                                                                                                                                                                >  1:82019/1‑250 (MQ=255)                          aCCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTACCCGCTGAATGGCTGACTTCTGACACCAAATTCTTCATAAACCCGAACGGTCGTTTCGTTATAGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATAACTAAGGAGGCATGGCGCGTCACGGTGGCGGAGCATTCTCTGGTAAAGATCTATCACAAGTGGACCGTTCCGAAGCCTCCgc                                                                                                                                                                                                                >  1:159437/1‑250 (MQ=255)                                aaTCGCTACAAGAAGAGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTAACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCTATCAAAAGTGGACCGTTCCTCCGCCTACACAGCACGt                                                                                                                                                                                                         >  2:220363/1‑251 (MQ=255)                                 aTCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCTATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACGtt                                                                                                                                                                                                        >  1:216157/1‑251 (MQ=255)                                                      ggAAGAGACCATCAAGCCAATTCTGCCCGCGGAATGGCTGACTTCTGCCCCCAAATTCTTCATCAACCCGACCGGTCGTGTCGTTATCGGTGGCCAAAGGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCGCTGGTAAAGATCTATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACGTTATGTCGCGAAAAACATCGTtg                                                                                                                                                                                   <  2:280221/251‑1 (MQ=255)                                                        aaGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCTATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACGTTATGTCGCGAAAAACATCGTtgct                                                                                                                                                                                 <  1:145953/251‑1 (MQ=255)                                                          gagaTCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCTATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACGTTATGTCGCGAAAAACATCGTtgctgc                                                                                                                                                                               >  2:91874/1‑251 (MQ=255)                                                           agaTCATCAAGCCAAGTCTGCCCGCTGAATGGCTGACGTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGGCGTAAAATTATCGTTGAGACCTACGGCGGCATTGCGCGTCACGGTGGCGGTGCATTCGCTGGTAAAGGTCTATCAAAAGTGGACCGTTGCGCAGCCTACGCAGCTCGTTATGTCGCGAAAAACATCGTTgctgct                                                                                                                                                                              <  1:291861/251‑1 (MQ=255)                                                                  caAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCTATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACGTTATGTCGCGAAAAACATCGTTGCTGctggcctgg                                                                                                                                                                       >  1:204678/1‑251 (MQ=255)                                                                     gCCAATTCGGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGGTTCGTTAGCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCTATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCTCGTTATGTCGCGAAAAACATCGTTGCTGCGGGCCTGGCCg                                                                                                                                                                    <  2:118929/251‑1 (MQ=255)                                                                             tGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCACAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATCGCGCGTCACGGTGGCGGTGCCTTCTCTGGTAAAGATCTATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCCCGTTATGTCGCGAAAACCATCGTTGCTGCTGGCCTGGCCGATCGtttt                                                                                                                                                            >  2:173308/1‑249 (MQ=255)                                                                              gCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCTATCAAAATTGGACCGTTCCGCAGCCTACGCAGCACGTTATGTCGCGAAAAACATCGTTGCTGCTGGCCTGGCCGATCGTtg                                                                                                                                                             <  1:211802/249‑1 (MQ=255)                                                                              gCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCTATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACGTTATGTCGCGAAAAACATCGTTGCTGCTGGCCTGGCCGATCGTtgt                                                                                                                                                            <  2:151632/250‑1 (MQ=255)                                                                                ccGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCTATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACGTTATGTCGCGAAAAACATCGTTGCTGCTGGCCTGGCCGCTCGTTGTGa                                                                                                                                                          >  2:275287/1‑250 (MQ=255)                                                                                  gCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCTATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACGTTATGTCGCGAAAAACATCGTTGCTGCTGTCCTGGCCGATCGTTGGGAAAt                                                                                                                                                       >  1:6304/1‑251 (MQ=255)                                                                                   cTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCACAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCTATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACGTTATGTCGCGAAAAACATCGTTGCTGCTGGCCTGGCCGATCGTTGTGAAAtt                                                                                                                                                      >  1:309535/1‑251 (MQ=255)                                                                                             gACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTCTCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCTATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACGTTATGTCGCGAAAAACATCGTTGCTGCTGGCCTGGCCGATCGTTGTGAAATTCAGGTTTCCt                                                                                                                                            >  1:200491/1‑251 (MQ=255)                                                                                             gACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCTATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACGTTATGTCGCGAAAAACATCGTTGCTGCTGGCCTGGCCGATCGTTGTGAAATTCAGGTTTcc                                                                                                                                             <  2:133911/250‑1 (MQ=255)                                                                                                ttCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAACTTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCTATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACGTTATGTCGCGAAAAACATCGTTGCTGCTGGCCTGGCCGATCGTTGTGAAATTCAGGTTTCCTACg                                                                                                                                         >  1:157857/1‑251 (MQ=255)                                                                                                  cTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGCCTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGAGCATTCTCTGGTAAAGATCTTTCAAAAGTGGACCGTTACGCAGCCTACGCAGCACGATATGTCGCGAAAAAAATCGTTGCTGCCGGCCTGGCCGATCGTTGAGAAATTCAGCTTTCCTACGc                                                                                                                                        >  1:24283/1‑250 (MQ=255)                                                                                                         caAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCTATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACGTTATGTCGCGAAAAACATCGTTGCTGCTGGCCTGGCCGATCGTTGTGAAATTCAGGTTTCCTACGCAATCGGCg                                                                                                                                >  1:276197/1‑251 (MQ=255)                                                                                                           aaTTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCTATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACGTTATGTCGCGAAAAACa                                                                                                                                                                                         >  2:94059/1‑192 (MQ=255)                                                                                                           aaTTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCTATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACGTTATGTCGCGAAAAACa                                                                                                                                                                                         <  1:94059/192‑1 (MQ=255)                                                                                                                 tcatcaACCCGACCGGTCGATTCGTTATCGGTGGCCCCATCGGTGACTGCGGTCTGCCTGGTCGTCAAATTCTCGATCATACCTACGCCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGCTCTATCAAAAATGGCCCGTTCCCCAGCCTACGCAGCACGTTATGTCGCCAAAAACATCGTTGCTGCTCGCCTGGCAGAACGTTGGGAAATTAATGTTTCCTCAGCAATCGGccgtgctga                                                                                                                         >  2:193027/1‑242 (MQ=255)                                                                                                                     caACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTTGGCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCTATCAAAAGTGGACCGTTCCGCAGCCTTCGCAGCACGTTATGTCGCGAAAAAGATCGTTGCTGCTGGCCTGGCCGATCGTTGTGAAATTCAGGTTTCCTACGCAAGCGGCGTGGCTGAACCGa                                                                                                                    <  1:210474/251‑1 (MQ=255)                                                                                                                      aaCCCGACCGGACGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCTATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACGTTATGTCGCGAAAAACATCGTTGCTGCTGGCCTGGCCGATCGTTGTGAAATTCAGGTTTCCTACGCAATCGGCGTGGCTGAACCGAc                                                                                                                   >  1:268176/1‑251 (MQ=255)                                                                                                                        cccGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCTATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACGTTATGTCGCGAAAAACATCGTTGCTGCTGGCCTGGCCGATCGTTGTGAAATTCAGGTTTCCTACGCAATCGGCGTGGCTGAACCGACCt                                                                                                                 <  2:67611/251‑1 (MQ=255)                                                                                                                          cGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCTATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACGTTATGTCGCGAAAAACATCGTTGCTGCTGGCCTGGCCGATCGTTGTGAAATTCAGGTTTCCTACGCAATCGGCGTGGCTGAACCGACCTcc                                                                                                               >  2:83265/1‑251 (MQ=255)                                                                                                                                gTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAACTTATCGTT‑ATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCTATCAAAAGTGGACCGTTCCGCCGCCTACGCAGCACGTTAAGTCGCGAAAAACATCGTTGCTGCTGGCCTGGCCGATCGTTGTGAAATTCATGTTTCCTACGCAATCGGCGTGGCTGACCCGGCCACCATCATgg                                                                                                        >  2:280230/1‑251 (MQ=255)                                                                                                                                     ttcgttATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCTATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACGTTATGTCGCGAAAAACATCGTTGCTGCTGGCCTGGCCGATCGTTGTGAAATTCAGGTTTCCTACGCAATCGGCGTGGCTGAACCGACCTCCATCATGGt                                                                                                       >  1:4191/1‑248 (MQ=255)                                                                                                                                           aTCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCTATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCCCGTTATGTCGCGAAAAACATCGTTGCTGCTGGCCTGGCCGATCGTTGTGAAATTCAGGTTTCCTACGCAATCGGCGTGGCTGAACCGACCTCCATCATGGTAGAAACttt                                                                                              >  2:325695/1‑251 (MQ=255)                                                                                                                                                                            ggTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCTATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACGTTATGTCGCGAAAAACATCGTTGCTGCTGGCCTGGCCGATCGTTGTGAAATTCAGGTTTCCTACGCAATCGGCGTGGCTGAACCGACCTCCATCATGGTAGAAACTTTCGGTACTGAGAAAGTGCCTTCTGAACAACTg                                                               <  1:238055/249‑1 (MQ=255)                                                                                                                                                                               cGTAAAATTATCGTTTATACCTACTGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCTATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACGTTATGTCGCGAAAAACATCGTTGCTGCTGGCCTGGCCGATCGTTGTGAAATTCAGGTTTCCTACGCAATCGGCGTGGCTGAACCGACCTCCATCATGGTAGAAACTTTCGGTACTGAGAAAGTGCCTTCTGAACAACTGAcct                                                           <  2:25271/250‑2 (MQ=255)                                                                                                                                                                                   aaaTTAGCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCTATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACGTTATGTCGCGAAAAACATCGTTGCTTCTGGCCTGGCCGATCGTTGTGAAATTCAGGTTTCCTACGCAATCGGCGTGGCTGAACCGACCTCCATCATGGTAGAAACTTTCGGTACTGAGAAAGTGCCTTCTGAACAACTGACCctgctg                                                      <  2:309535/251‑1 (MQ=255)                                                                                                                                                                                           gTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCTATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACGTTATGTCGCGAAAAACATCGTTGCTGCTGGCCTGGCCGATCGTTGTGAAATTCAGGTTTCCTACGCAATCGGCGTGGCTGAACCGACCTCCATCATGGTAGAAACTTTCGGTACTGAGAAAGAGCCTTCTGAACAACTGACCCTGCTGGTACGTg                                               >  1:115670/1‑250 (MQ=255)                                                                                                                                                                                               aTACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCTATCAAAAGGTGACCGTTCCGCAGCCTACGCAGCACGTTATGTCGCGAAAAACATCGTTGCTGCTGGCCTGGCCGATCGTTGTGAAATTCAGGTTTCCTACGCAATCGGCGTGGCTGAACCGACCTCCATCATGGTAGAAACTTTCGGTACTGAGAAAGTGCCTTCGGAACAACTGGCCCTGCTGGTACGTGAGttc                                          <  2:200491/251‑1 (MQ=255)                                                                                                                                                                                                     aCGGCGGCAGGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCTATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACGTTATGTCGCGAAAAACATCGTTGCTGCTGGCCTGGCCGATCGTTGTGAAATTCAGGTTTCCTACGCAATCGGCGTGGCTGAACCGACCTCCATCATGGTAGAAACTTTCGGTACTGAGAAAGTGCCTTCTGAACAACTGACCCTGCTGGTACGTGAGTTCTTCGAc                                    <  2:204678/251‑1 (MQ=255)                                                                                                                                                                                                            cATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCTATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACGTTATGTCGCGAAAAACATCGTTGCTGCTGGCCTGGCCGATCGTTGTGAAATTCAGGTTTCCTACGCAATCGGCGTGGCTGAACCGACCTCCATACTGGTAGAAACTTTCGGTACTGAGAAAGTGCCTTCTGAACCACTGAACCTGCTGGTACGTGAGTTCTTCGACCTGCGcc                             >  2:90268/1‑251 (MQ=255)                                                                                                                                                                                                                                      tctcTGGTAAAGATCTATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACGTTATTTCGCGAAAAACATCGTTGCTGCTGGCCTGGCCGATCGTTGTGAAATTCAGGTTTCCTACGCAATCGGCGTGGCTGAACCGACCTCCATCATGGTAGAAACTTTCGGTACTGAGAAAGTGCCTTCTGAACAACTGACCCTGCTGGTACGTGAGTTCTTCGACCTGCGCCCATACGGTCTGATTCAGATGCTGGa    <  1:6536/250‑1 (MQ=255)                                                                                                                                                                                                                                       ctctGGTAAAGATCTATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACGTTATGTCGCGAAAAACATCGTTGCTGCTGGCCTGGCCGATCGTTGTGAAATTCAGGTTTCCTACGCAATCGGCGTGGCTGAACCGACCTCCATCATGGTAGAAACTTTCGGTACTGAGAAAGTGCCTTCTGAACAACTGACCCTGCTGGTACGTGAGTTCTTCGACCTGCGCCCATACGGTCTGATTCAGATGCTGGATc  >  1:31226/1‑251 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                             TCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACGTTATGTCGCGAAAAACATCGTTGCTGCTGGCCTGGCCGATCGTTGTGAAATTCAGGTTTCCTACGCAATCGGCGTGGCTGAACCGACCTCCATCATGGTAGAAACTTTCGGTACTGAGAAAGTGCCTTCTGAACAACTGACCCTGCTGGTACGTGAGTTCTTCGACCTGCGCCCATACGGTCTGATTCAGATGCTGGATC  >  NC_000913/3087264‑3087744

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑