Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,023,559	A→G	T223A (ACT→GCT)	fliR →	flagellar biosynthesis protein FliR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,023,559	0	A	G	100.0%	36.4 / NA	12	T223A (ACT→GCT)	fliR	flagellar biosynthesis protein FliR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (3/9);  total (3/9)

GGTCATTTATGGTTGATTTCACTGCTGGTCGATACCTTTCACACCCTGCCGATTGGTGGCGAACCGTTGAACAGCAATGCGTTTCTGGCACTCACCAAAGCAGGGAGTTTGATTTTCCTTAACGGGCTGATGCTGGCGTTACCGCTCATTACTCTGCTGCTGACACTGAATCTGGCATTAGGTTTACTTAATCGTATGGCCCCGCAATTATCCATTTTTGTTATTGGATTTCCATTAACTCTGACTGTCGGCATCTCTTTAATGGCGGCATTAATGCCGTTAATTGCACCTTTTTGCGAACATTTATTCAGTGAAATTTTTAATTTGCTGGCTGATATTATTAGTGAATTGCCATTAATATAATTCCGTAACGTTTATCATGTTATCCTAAGGATTATCCGAAAAATAATACCTACGAACATCTTCCAGGATACTCCTGCAGCGAAATATTTGTTTTAAGCTCACTCA  >  NC_000913/2023316‑2023783                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                 ggTCATTTATGGTTGATTTCACTGCTGGTCGATACCTTTCACACCCTGCCGATTGGTGGCGAACCGTTGAACAGCAATGCGTTTCTGGCACTCACCAAAGCAGGGAGTTTGATTTTCCTTAACGGGCTGATGCTGGCGTTACCGCTCATTACTCTGCTGCTGACACTGAATCTGGCATTAGGTTTACTTAATCGTATGGCCCCGCAATTATCCATTTTTGTTATTGGATTTCCATTAACTCTGGCTGTCgg                                                                                                                                                                                                                           <  1:10018/251‑1 (MQ=255)    cATTTATGGTTGATTTCACTGCTGGTCGATACCTTTCACACCCTGCCGATTGGTGGCGAACCGTTGAACAGCAATGCGTTTCTGGCACTCACCAAAGCAGGGAGTTTGATTTTCCTTAACGGGCTGATGCTGGCGTTACCGCTCATTACTCTGCTGCTGACACTGAATCTGGCATTAGGTTTACTTAATCGTATGGCCCCGCAATTATCCATTTTTGTTATTGGATTTCCATTAACTCTGGCTGTCGGCa                                                                                                                                                                                                                         <  1:83985/250‑1 (MQ=255)                                     tttCACACCCTGCCGATTGGTGGCGAACCGTTGAACAGCAATGCGTTTCTGGCACTCACCAAAGCAGGGCGTTTGATTTTCCTTAACGGGCTGATGCTGGCGTTACCGCTCATTACTCTGCTGCTGACACTGAATCTGGCATTAGGTTTACTTAATCGTATGGCCCCGCAATTATCCATTTTTGTTATTGGATTTCCATTAACTCTGGCTGTCGGCATCTCTTTAATGGCGGCATTAATGCCGTTAATTGc                                                                                                                                                                                       >  1:50896/1‑251 (MQ=255)                                          cacCCTGCCGATTGTTGGCGAACCGTTGAACAGCAATGCGTTTCTGGCACTCACCAAAGCAGGGAGTTTGATTTTCCTTAACGGGCTGATGCTGGCGTTACCGCTCATTACTCTGCTGCTGACACTGAATCTGGCATTAGGTTTACTTAATCGTATGGCCCCGCAATTATCCATTTTTGTTAGTGGATTTCCATTAACTCTGGCTGTCGGCATCTCTTTAATGGCGGCATTAATGCCGTTAATTGCACCtt                                                                                                                                                                                  <  2:304735/251‑1 (MQ=255)                                             ccTGCCGATTGGTGGCGAACCGTTGAACAGCAATGCGTTTCTGGCACTCACCAAAGCAGGGAGTTTGATTTTCCTTAACGGGCTGATGCTGGCGTTACCGCTCATTACTCTGCTGCTGACACTGAATCTGGCATTAGGTTTACTTAATCGTATGGCCCCGCAATTATCCATTTTTGTTATTGGATTTCCATTAACTCTGGCTGTCGGCATCTCTTTAATGGCGGCATTAATGCCGTTAATTGCACCttttt                                                                                                                                                                               >  1:202038/1‑251 (MQ=255)                                                                           cAATGCGTGTCTGGCACTCACCAAAGCAGGGAGTTTGATTTTCCTTAACGGGCTGATGCTGGCGTTACCGCTCATTACTCTGCTTCGGACACTGAATCTGGCATTATGTTTACTTAATCGTATGGCCCCGCAATTATCCATTTTTGTTATTGGATTTCCATTAACTCTGGCTGTCGGCATCTCTTTAATGGCGGCAGTAATGTCGTTAATTGCACCTTTTTGCGAACATTTATTCAGTGAAATTTTTAAtt                                                                                                                                                 <  2:202038/251‑1 (MQ=255)                                                                                          aCTCACCAAAGCAGGGAGTTTGATTTTCCTTAACGGGCTGATGCTGGCGTTACCGCTCATTACTCTGCTGCTGACACTGAATCTGGCATTAGGTTTACTTAATCGTATGGCCCCGCAATTATCCATTTTTGTTATTGGATTTCCATTAACTCTGGCTGTCGGCATCTCTTTAATGGCGGCATTAATGCCGTTAATTGCACCTTTTTGCGAACATTTATTCAGTGAAATTTTTAATTTGCTGGCTGAtatta                                                                                                                                  <  2:99358/251‑1 (MQ=255)                                                                                                         gAGTTTGATTTTCCTTAACGGGCTGATGCTGGCGTTACCGCTCATTACTCTGCTGCTGACACTGAATCTGGCATTAGGTTTACTTAATCGTATGGCCCCGCAATTATCCATTTTTGTTATTGGATTTCCATTAACTCTGGCTGTCGGCATCTCTTTAATGGCGGCATTAATGCCGTTAATTGCACCTTTTTGCGAACATTTATTCAGTGAAATTTTTAATTTGCTGGCTGATATTATTAGTGAATTGCCAt                                                                                                                   <  1:4272/251‑1 (MQ=255)                                                                                                                                               cGCTCATTACTCTGCTGCTGACACTGAATCTGGCATTAGGTTTACTTAATCGTATGGCCCCGCAATTATCCATTTTTGTTATTGGATTTCCATTAACTCTGGCTGTCGGCATCTCTTTAATGGCGGCATTAATGCCGTTAATTGCACCTTTTTGCGAACATTTATTCAGTGAAATTTTTAATTTGCTGGCTGATATTATTAGTGAATTGCCATTAATATAATTCCGTAACGTTTATCATGTTATCCTAAgg                                                                             >  1:193657/1‑251 (MQ=255)                                                                                                                                                                                                                  aTCCATTTTTGTTATTGGATTTCCATTAACTCTGGCTGTCGGCATCTCTTTAATGGCGGCATTAATGCCGTTAATTGCACCTTTTTGCGAACATTTATTCAGTGAAATTTTTAATTTGCTGGCTGATATTATTAGTGAATTGCCATTAATATAATTCCGTAACGTTTATCATGTTATCCTAAGGATTATCCGAAAAATAATACCTACGAACATCTTCCAGGATACTCCTGCAGCGAAATATTTGTTTTaa           <  1:84250/250‑1 (MQ=255)                                                                                                                                                                                                                    ccATTTTTGTTATTGGATTTCCATTAACTCTGGCTGTCGGCATCTCTTTAATGGCGGCATTAATGCCGGTAATTGCACCTTTTTGCGAACATTTATTCAGTGAAATTTTTAATTTGCTGGCTGATATTATTAGTAAATTGCCATTAATATAATTCCGTAACGTTTATCATTTTATCCTAAGGATGATCCGAAAAATAATACCTACGAACATCTTCCAGGATACTCCTGCAGCGAAATATTTGTTTTAAGct        <  1:145435/251‑1 (MQ=255)                                                                                                                                                                                                                           tGTTATTGGATTTCCATTAACTCTGGCTGTCGTCATCTCTTTAATGGCGGCATTAATGCCGTTAATTGCACCTTTTTGCGAACATTTATTCAGTGAAATTTTTAATTTGCTGGCTGATATTATTAGTGAATTGCCATTAATATAATTCCGTAACGTTTATCATGTTATCCTAAGGATTATCCGAAAAATAATACCTACGAACATCTTCCAGGATACTCCTGCAGCGAAATATTTGTTTTAAGctcactca  <  2:265178/250‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                 GGTCATTTATGGTTGATTTCACTGCTGGTCGATACCTTTCACACCCTGCCGATTGGTGGCGAACCGTTGAACAGCAATGCGTTTCTGGCACTCACCAAAGCAGGGAGTTTGATTTTCCTTAACGGGCTGATGCTGGCGTTACCGCTCATTACTCTGCTGCTGACACTGAATCTGGCATTAGGTTTACTTAATCGTATGGCCCCGCAATTATCCATTTTTGTTATTGGATTTCCATTAACTCTGACTGTCGGCATCTCTTTAATGGCGGCATTAATGCCGTTAATTGCACCTTTTTGCGAACATTTATTCAGTGAAATTTTTAATTTGCTGGCTGATATTATTAGTGAATTGCCATTAATATAATTCCGTAACGTTTATCATGTTATCCTAAGGATTATCCGAAAAATAATACCTACGAACATCTTCCAGGATACTCCTGCAGCGAAATATTTGTTTTAAGCTCACTCA  >  NC_000913/2023316‑2023783

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑