Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,296,481	G→A	V25M (GTG→ATG)	dolP →	division and outer membrane stress‑associated lipid‑binding lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,296,481	0	G	A	97.0%	213.8 / NA	67	V25M (GTG→ATG)	dolP	division and outer membrane stress‑associated lipid‑binding lipoprotein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (38/27);  minor base C (2/0);  total (40/27)
Fisher's exact test for biased strand distribution p-value = 5.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

ACCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCCGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAGTGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCAAAGTGCTGCTGGTTGGGCAGTCACCAAATGCTGAACTTTCGGCTCGCGCCAAACAGATTGCTATGGGCGTAGACGGTGCCAACG  >  NC_000913/3296235‑3296724                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    aCCGGATATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCCGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGTCGGCAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATggg                                                                                                                                                                                                                                                 >  2:149078/1‑251 (MQ=255)       tATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCCGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGACGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCgc                                                                                                                                                                                                                                           >  2:303591/1‑251 (MQ=255)         tGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCCACATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGGAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCACGGTTGTGTTGCCGCTGCCGTACTGGGTACCgctg                                                                                                                                                                                                                                         >  1:178814/1‑251 (MQ=255)          gACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCCGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCgctg                                                                                                                                                                                                                                         <  1:320601/250‑1 (MQ=255)                      cgtGCAGGTTTGGTAGGGCCACAGGATGTGGAGATCCGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCGGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCaa                                                                                                                                                                                                                            <  2:121981/249‑1 (MQ=255)                            ggTTTGTTAGGGCCACAGGATGTGGAGATCCGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGc                                                                                                                                                                                                                      >  2:103620/1‑251 (MQ=255)                                               aTGTGGAGATCCGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTc                                                                                                                                                                                                   <  1:273984/251‑1 (MQ=255)                                                   ggAGATCCGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGCGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGcc                                                                                                                                                                                               >  2:193132/1‑250 (MQ=255)                                                        tCCGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCAccc                                                                                                                                                                                            >  1:34213/1‑249 (MQ=255)                                                           gCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTg                                                                                                                                                                                       >  1:229792/1‑251 (MQ=255)                                                             aTTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCTCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTgg                                                                                                                                                                                      >  1:45505/1‑250 (MQ=255)                                                             aTTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCAAGGTgg                                                                                                                                                                                      >  2:230340/1‑250 (MQ=255)                                                               tCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGAc                                                                                                                                                                                    <  2:78341/250‑1 (MQ=255)                                                                   tCGCATCGTAGTGCTCGCATTCAGGAAATGCAGATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATg                                                                                                                                                                                <  2:16383/250‑1 (MQ=255)                                                                           tAGTGCGCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTCAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGATGCAAGGTTGTGTTGCCGCTGCCGTAATGGTTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTgg                                                                                                                                                                       >  2:157823/1‑251 (MQ=255)                                                                             gTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCGGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCTGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGaa                                                                                                                                                                     <  2:27143/251‑1 (MQ=255)                                                                               gCTCGCCTTCAGGAAATGCATATGCTGACGGTAACTTGCCTGTGCGATCTGATCGATAACACGCTTTTCCATCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTAccc                                                                                                                                                                          >  2:296055/1‑244 (MQ=255)                                                                               gCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTAccc                                                                                                                                                                          <  1:296055/244‑1 (MQ=255)                                                                                   gCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGAGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTgcgc                                                                                                                                                               >  1:327902/1‑251 (MQ=255)                                                                                    cATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGcgcg                                                                                                                                                              >  2:42069/1‑251 (MQ=255)                                                                                     aTTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTACGAAGAATACATGCAGGCATTATCGCCAATCGCAGTCCTTGTTTCCACGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAGGCCGCCACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTgcgagg                                                                                                                                                             >  1:151921/1‑248 (MQ=255)                                                                                     aTTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGCACCCTGGCAGTGCGCGt                                                                                                                                                             >  2:273219/1‑251 (MQ=255)                                                                                            aaaTGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAgc                                                                                                                                                      <  1:103073/251‑1 (MQ=255)                                                                                              aTGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGAAAGGATGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGGACCAAAGCCGCAACTGACCCCCGCAGTGTCGGCACCCAGGTGGACGTTGGGACCCTGGAAGTGCGCGTGAACAgcgc                                                                                                                                                    >  2:180947/1‑251 (MQ=255)                                                                                               ggCATATGCTGACGTGAAATTGCCTGTGCGAGCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTTGCGCCAATCGCAGTCCTTATTTCCGCGCTGCGGTTGCAAGGTTGGGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCGGGAAGTGCTCGGGAACAGCGCa                                                                                                                                                   <  2:141691/250‑1 (MQ=255)                                                                                                 catatccggACGGTAAATTGCCTGTGCGATCTGATCGAGAACACGCTTTTCCCTCACCAGGATGAGTAAGGAGAATACATGAAGTCATTATCGCCAATCGCAGTCCTTATGTCCGCGCGGCTGTTGCAAGGTTGTGTTTCCGCTGCCGTAATGGGTACCGCTGCTGGGGGGACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGTAAGTGCGCGTGAACACCGCAtt                                                                                                                                                 <  2:151921/243‑1 (MQ=255)                                                                                                   tatGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGt                                                                                                                                               >  2:187153/1‑251 (MQ=255)                                                                                                    atGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGAGTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTc                                                                                                                                              <  1:209583/251‑1 (MQ=255)                                                                                                      gCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTGGCCTCTGCCGTAATGGGTACCGCGGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCg                                                                                                                                             <  2:178814/250‑1 (MQ=255)                                                                                                             gTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGa                                                                                                                                     <  2:305548/251‑1 (MQ=255)                                                                                                               aaaTTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAAc                                                                                                                                   >  1:223201/1‑251 (MQ=255)                                                                                                                aaTTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAAc                                                                                                                                   >  2:14716/1‑250 (MQ=255)                                                                                                                      cTGTGCGATCTGATCGATAACACGCTTTTACCTAACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGCTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCCGTGTCGGAACCCAGGTGGACGATGGTACCCTGGAAATGCGCGTGACCAGCGCATTGTCGAACGCCGAACAGATTa                                                                                                                             >  2:313236/1‑250 (MQ=255)                                                                                                                            gATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTaagaa                                                                                                                         >  1:125624/1‑248 (MQ=255)                                                                                                                                tGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAgcg                                                                                                                  >  1:237289/1‑251 (MQ=255)                                                                                                                                 gATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTaagaaaga                                                                                                                      <  2:104059/246‑1 (MQ=255)                                                                                                                                 gATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAgcgc                                                                                                                 <  1:42069/251‑1 (MQ=255)                                                                                                                                 gATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAgcgc                                                                                                                 >  2:262255/1‑251 (MQ=255)                                                                                                                                  aTCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAgcgcg                                                                                                                <  1:8916/251‑1 (MQ=255)                                                                                                                                   tcgatcaaaagCTTTTCCCTCACCAGGATGATTACGGAGAATCCATGAAGGCATAATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGAGCCACGCAGTGTCGGCACCCAGGGGGACGATGGTACCCTGGAAGTGCGCGTGACCAGCGCATTGTCGCAAGACGAACAGATTAAGAAAGAAgagcgc                                                                                                               >  2:297130/11‑251 (MQ=255)                                                                                                                                    cGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAgcgcgc                                                                                                               >  1:133692/1‑250 (MQ=255)                                                                                                                                            cGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATg                                                                                                        <  1:90615/249‑1 (MQ=255)                                                                                                                                               ttttCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATAGCCAATCGCAGTCCTTATTTCCACGCTGCTGTTGCCAGCATGAGTTGCCGCTGCCGTACTGGGTACCGCTACTAAGGGTACAAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGAGCCGGCAGGTACCCTGGCAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACg                                                                                                   >  1:23039/1‑251 (MQ=255)                                                                                                                                                   cccTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCt                                                                                               >  2:240405/1‑251 (MQ=255)                                                                                                                                                    ccTCACCAGGATGATTAAGGAGAATACAGGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTa                                                                                              <  1:156106/251‑1 (MQ=255)                                                                                                                                                         ccAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAgg                                                                                         >  2:27961/1‑251 (MQ=255)                                                                                                                                                           aGGATGATTAAGGAGAATCCATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGc                                                                                       >  1:59647/1‑251 (MQ=255)                                                                                                                                                            ggATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGc                                                                                       >  1:91205/1‑250 (MQ=255)                                                                                                                                                            ggATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGc                                                                                       >  1:299518/1‑250 (MQ=255)                                                                                                                                                              atgatTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTGCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGGCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCAGTAATGTAACGGCCTATCAGGGCaaa                                                                                    <  2:327902/251‑1 (MQ=255)                                                                                                                                                                gatTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCAAAg                                                                                   <  1:272382/250‑1 (MQ=255)                                                                                                                                                                   tAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCAAAGtgct                                                                               >  1:180466/1‑251 (MQ=255)                                                                                                                                                                                   aGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCAAAGTGCTGCTGGTTGGGCAGTCa                                                               <  1:303591/251‑1 (MQ=255)                                                                                                                                                                                            cGCCACTCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCAAAGTGCTGCTGGTTGGGCAGTCACCAAATGCt                                                      >  2:150016/1‑251 (MQ=255)                                                                                                                                                                                             gCCAATCGCAGTCCTTATTTCCGCGATGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGAGCGCGTGAACAGCGCATTGTCGAAAGACGAAAAGATTAAGAAAGAAGCGCGCATTAATGTCACGGCCTATCAGGGCAAAGTGCTGCTGGTTGGGCAGTCACCAAATGCTg                                                     >  2:18982/1‑251 (MQ=255)                                                                                                                                                                                                 aTCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGCAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCAAAGTGCTGCTGGTTGGGCAGTCACCAAATGCTGCACt                                                 >  2:275981/1‑251 (MQ=255)                                                                                                                                                                                                      aGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGCACCAAAGCCGCAACTGACCCACGCAGTGTCGGAACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGACAGCCGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCAAAGTGCTGCTGGTTGGGCAGTCACCAAATGCTGAACTTTCgg                                            >  1:294816/1‑251 (MQ=255)                                                                                                                                                                                                          cTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCAAAGTGCTGCTGGTTGGGCAGTCACCAAATGCTGAACTTTCGGCTcg                                        >  1:325037/1‑251 (MQ=255)                                                                                                                                                                                                           ttATTTCCGCGATGCTGTTGCAAGGTTGTGTTGTCGCTGCCGTAATGGGTACCGCTGCTGTGGGTCCCCAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGTGCGTGATCAGCGCATTGTCGAAAGACGAACAGATTCAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCAAAGTGCTGCTGGTTGGGCAGTCACCAACAGCTGAACTTTCGGCTcgc                                       >  2:161825/1‑251 (MQ=255)                                                                                                                                                                                                             aTTTCCGCGCAGCTGTTGCAAGATTGTGTTGCCGCTGCCGTAATGGGTACCGCTGATGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATCGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGACAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCAAAGTGCTGCTGGTTGGGCAGTCACCAAATGCTGAACTTTCGGCTCgcgc                                     >  2:142532/1‑251 (MQ=255)                                                                                                                                                                                                                   gcgcTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGGAATGTGTTCCGCTGCTGGGGGTACCAAAGCCGCAACGGACCCACGCAGTGTCGGCACCCAGGTGGACGATTGTACCCTGGAAGTGCGCTTGAACAGCGCACTGTCGAAAGTCGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCAAAGTGCTGCGGGCAGGGCAGTCACCAAATGCTGAACTTTCGGCTCGCGCCAAACa                               <  2:291729/251‑1 (MQ=255)                                                                                                                                                                                                                     gctgctGTTGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCAAAGTGCTGCTGGTTGGGCAGTCACCAAATGCTGAACTTTCGGCTCGCGCCAAACAGa                             <  2:68300/251‑1 (MQ=255)                                                                                                                                                                                                                             tGCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCAAAGTGCTGCTGGTTGGGCAGTCACCAAATGCTGAACTTTCGGCTCGCGCCAAACAGATTGCTATg                     >  1:261055/1‑251 (MQ=255)                                                                                                                                                                                                                              gCAAGGTTGTGTTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCAAAGTGCTGCTGGTTGGGCAGTCACCAAATGCTGAACTTTCGGCTCGCGCCAAACAGATTGCTATgg                    <  2:196438/251‑1 (MQ=255)                                                                                                                                                                                                                                      gtgtTGCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCAAAGTGCTGCTGGTTGGGCAGTCACCAAATGCTGAACTTTCGGCTCGCGCCAAACAGATTGCTATGGGCGTAGAc            <  1:289423/251‑1 (MQ=255)                                                                                                                                                                                                                                           gCCGCTGCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCAAAGTGCTGCTGGTTGGGCAGTCACCAAATGCTGAACTTTCGGCTCGCGCCAAACAGATTGCTATGGGCGTAGACGGTGc       <  1:6810/251‑1 (MQ=255)                                                                                                                                                                                                                                                 gCCGTAATGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCAAAGTGCTGCTGGTTGGGCAGTCACCAAATGCTGAACTTTCGGCTCGCGCCAAACAGATTGCTATGGGCGTAGACGGTGCCAACg  <  1:109334/250‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    ACCGGCTATGACGGCGGCGAACTTGCAGGTTTGTTAGGGCCACAGGATGTGGAGATCCGCATTCCTTCGCATCGTAGTGCTCGCATTCAGGAAATGCATATGCTGACGGTAAATTGCCTGTGCGATCTGATCGATAACACGCTTTTCCCTCACCAGGATGATTAAGGAGAATACATGAAGGCATTATCGCCAATCGCAGTCCTTATTTCCGCGCTGCTGTTGCAAGGTTGTGTTGCCGCTGCCGTAGTGGGTACCGCTGCTGTGGGTACCAAAGCCGCAACTGACCCACGCAGTGTCGGCACCCAGGTGGACGATGGTACCCTGGAAGTGCGCGTGAACAGCGCATTGTCGAAAGACGAACAGATTAAGAAAGAAGCGCGCATTAATGTAACGGCCTATCAGGGCAAAGTGCTGCTGGTTGGGCAGTCACCAAATGCTGAACTTTCGGCTCGCGCCAAACAGATTGCTATGGGCGTAGACGGTGCCAACG  >  NC_000913/3296235‑3296724

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑