Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,476,605	G→A	S390N (AGC→AAC)	yjgL →	protein YjgL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,476,605	0	G	A	100.0%	50.8 / NA	15	S390N (AGC→AAC)	yjgL	protein YjgL
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/7);  total (8/7)

TGAAGAAATAGATAAGCTCTACGCATTAAATGATCACGGAATTGATAATGACAAAGTAGGTAACAATGAAATTAATGACATCAAAGTTAACCTGTCTCATATTCTTATTGATTCCTTAGATGATGCAAAGGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAATCCCCATATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAGCATGCAATATTTTGATGATACTAAAAAGATAAAGCATGCATGCTCCGTAATAAATCATATTAATCTTCGCAGCGATCAGTCTAAAATAGCTGAGACATTATTTTTCAATCTCGATAAAGAACCCTATAAAAATAGCCCTGAATTACAGGGGTTGATTTGGAATAAGTTGGTTGTATATGTCAATGAATTTAACTTAAGTAATCGAGAAAAAACAAATTTAATACAAAGGCTATTTGATAATGT  >  NC_000913/4476378‑4476848                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                    tGAAGAAATAGATAAGCTCTACGCATTAAATGATCACGGAATTGATAATGACAAAGTAGGTAACAATGAAATTAATGACATCAAAGTTAACCTGTCTCATATTCTTATTGATTCCTTAGATGATGCAAAGGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAATCCCCATATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAACATGCAATATTTTGATGATACt                                                                                                                                                                                                                               >  1:109381/1‑250 (MQ=255)   aagaaATAGATAAGCTCTACGCATTAAATGATCACGGAATTGATAATGACAAAGTAGGTAACAATGAAATTAATGACATCAAGGTTAACCTGTCTCATATTCTTATTGATTCCTTAGATGATGCAAAGGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAATCCCCATATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAACATGCAATATTTTGATGATACTaaa                                                                                                                                                                                                                            <  1:68623/251‑1 (MQ=255)             tAAGCTCTACGCATTAAATGATCACGGAATTGATAATGACAAAGTAGGTAACAATGAAATTAATGACATCAAAGTTAACCTGTCTCATATTCTTATTGATTCCTTAGATGATGCAAAGGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAATCCCCATATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAACATGCAATATTTTGATGATACTAAAAAGATAAAgc                                                                                                                                                                                                                  >  2:104134/1‑251 (MQ=255)                                                         tAGGTAACAATGAAATTAATGACATCAACGTTAACCTGTATCATATTCTTATTGATTACTTAGATGATGCAAAGGTTAACCTTACACCGTTCATCGATTCGATTCTGGAGACTTTTTCAAAATCCCCATATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAACATGCAATATTTTGATGATACTAACAAGATAAAGCATGCATGCTCCGAAATATATCATATTAATATTATCAGCGATc                                                                                                                                                                        >  2:77318/1‑249 (MQ=255)                                                                                                ctcATATTCTTATTGATTCCTTAGATGATGCAAAGGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAATCCCCATATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAACATGCAATATTTTGATGATACTAAAAAGATAAAGCATGCATGCTCCGTAATAAATCATATTAATCTTCGCAGCGATCAGTCTAAAATAGCTGAGACATTATTTTTCAATCTCGATaa                                                                                                                                >  2:261698/1‑250 (MQ=255)                                                                                                                     tAGATGATGCAAAGGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAATCCCCATATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAACATGCAATATTTTGATGATACTAAAAAGATAAAGCATGCATGCTCCGTAATAAATCATATTAATCTTCGCAGCGATCAGTCTAAAATAGCTGAGACATTATTTTTCAATCTCGATAAAGAACCCTATAAAAATAGCCCt                                                                                                          >  1:226373/1‑251 (MQ=255)                                                                                                                        atgatgCAAAGGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAATCCCCATATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAACATGCAATATTTTGATGATACTAAAAAGATAAAGCATGCATGCTCCGTAATAAATCATATTAATCTTCGCAGCGATCAGTCTAAAATAGCTGAGACATTATTTTTCAATCTCGATAAAGAACCCTATAAAAATAGCCCTGa                                                                                                        >  1:50302/1‑250 (MQ=255)                                                                                                                               aaaGGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAATCCCCATATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAACATGCAATATTTTGATGATACTAAAAAGATAAAGCATGCATGCTCCGTAATAAATCATATTAATCTTCGCAGCGATCAGTCTAAAATAGCTGAGACATTATTTTTCAATCTCGATAAAGAACCCTATAAAAATAGCCCTGAATTACAgg                                                                                                >  1:34371/1‑251 (MQ=255)                                                                                                                                                                                            atTAATGATGTAAGAATACTGGATTGGTGTTTTAAGAAAAACATGCAATATTTTGATGATACTAAAAAGATAAAGCATGCATGCTCCGTAATAAATCATATTAATCTTCGCAGCGATCAGTCTAAAATAGCTGAGACATTATTTTTCAATCTCGATAAAGAACCCTATAAAAATAGCCCTGAATTACAGGGGTTGATGTGGAATAAGTTGGTTGTATATGTCAATGAATTGAACTTAAGTAATCGAGaaaa                                   <  2:323722/251‑1 (MQ=255)                                                                                                                                                                                                     gTAAGAATACTGGATTGGTGTTTTAATAAAAACATGCAATATTTTGATGATACTAAAAAGATAAAGCATGCATGCTCCGTAATAAATCATATTAATCTTCGCAGCGATCAGTCTAAAATAGCTGAGACATTATTTTTCAATCTCGATAAAGAACCCTATAAAAATAGCCCTGAATTACAGGGGTTGATTTGGAATAAGTTGGTTGTATATGTCAATGAATTTAACTTAAGTAATCGAGAAAAAACAAAtt                           <  2:34371/250‑1 (MQ=255)                                                                                                                                                                                                         gAATACTGGATTGGTGTTTTAATAAAAACATGCAATATTTTGATGATACTAAAAAGATAAAGCATGCATGCTCCGTAATAAATCATATTAATCTTCGCAGCGATCAGTCTAAAATAGCTGAGACATTATTTTTCAATCTCGATAAAGAACCCTATAAAAATAGCCCTGAATTACAGGGGTTGATTTGGAATAAGTTGGTTGTATATGTCAATGAATTTAACTTAAGTAATCGAGAAAAAACAAATTTAATa                      <  2:179721/251‑1 (MQ=255)                                                                                                                                                                                                           aTACTGGATTGGTGTTTTAATAAAAACATGCAATATTTTGATGATACTAAAAAGATAAAGCATGCATGCTCCGTAATAAATCATATTAATCTTCGCAGCGAACAGTCTAAAATAGCTGAGACATTATTTTTCAATCTCGATAAAGAACCCTATAAAAATAGCCCTGAATTACAGGGGTTGATTTGTAATAAGTTGGTTGTATATGTCAATGAATTTAACTTAATTAATCGAGAAAAAACAAATTTAATACa                    <  2:159367/251‑1 (MQ=255)                                                                                                                                                                                                             aCTGGATTGGTGTTTTAATAAAAACATGCAATATTTTGATGATACTAAAAAGATAAAGCATGCATGCTCCGTAATAAATCATATTAATCTTCGCAGCGATCAGTCTAAAATAGCTGAGACATTATTTTTCAATCTCGATAAAGAACCCTATAAAAATAGCCCTGAATTACAGGGGTTGATTTGGAATAAGTTGGTTGTATATGTCAATGAATTTAACTTAAGTAATCGAGAAAAAACAAATTTAATACaa                   <  2:248067/250‑1 (MQ=255)                                                                                                                                                                                                                ggATTGGTGTTTTAATAAAAACATGCAATATTTTGATGATACTAAAAAGATAAAGCATGCATGCTCCGTAATAAATCATATTAATCTTCGCAGCGATCAGTCTAAAATAGCTGAGACATTATTTTTCAATCTCGATAAAGAACCCTATAAAAATAGCCCTGAATTACAGGGGTTGATTTGGAATAAGTTGGTTGTATATGTCAATGAATTTAACTTAAGTAATCGAGAAAAAACAAATTTAATACAAAGGc               <  1:89348/251‑1 (MQ=255)                                                                                                                                                                                                                             aataaAAACATGCAATATTTTGATGATACTCAAAAGATAAAGCATGCATGCTCCGTAATAAATCATATTAATCTTCGCAGCGATCAGTCTAAAATAGCTGAGACATTATTTTTCAATCTCGATAAAGAACCCTATAAAAATAGCCCTGAATTACAGGGGTTGATTTGGAATAAGTTGGTTGTATATGTCAATGAATTTACCTTAAGTAATCGAGAAAAAACAAATTTAATACAAAGGCTATTTGATAATGt  >  2:47077/1‑251 (MQ=255)                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                    TGAAGAAATAGATAAGCTCTACGCATTAAATGATCACGGAATTGATAATGACAAAGTAGGTAACAATGAAATTAATGACATCAAAGTTAACCTGTCTCATATTCTTATTGATTCCTTAGATGATGCAAAGGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAATCCCCATATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAGCATGCAATATTTTGATGATACTAAAAAGATAAAGCATGCATGCTCCGTAATAAATCATATTAATCTTCGCAGCGATCAGTCTAAAATAGCTGAGACATTATTTTTCAATCTCGATAAAGAACCCTATAAAAATAGCCCTGAATTACAGGGGTTGATTTGGAATAAGTTGGTTGTATATGTCAATGAATTTAACTTAAGTAATCGAGAAAAAACAAATTTAATACAAAGGCTATTTGATAATGT  >  NC_000913/4476378‑4476848

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑