Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,167,148	G→A	H191Y (CAT→TAT)	ygiS ←	putative deoxycholate binding periplasmic protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,167,148	0	G	A	100.0%	113.6 / NA	30	H191Y (CAT→TAT)	ygiS	putative deoxycholate binding periplasmic protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/12);  total (18/12)

GCCGGAACCCAGGTGAGATCGACCTCTCCCGCGCGATAGCGGTTATAGCCGGTGACCGAATTATCTAGCGCCAGATACTCAACCTGTTGCAATACTGTATGTTGCGCATCGCGGTACTTTGGATTTTTGCGTGCAGTAATCTTTTCGTTAACTACCCACTGATCAAGCACAAAGGCACCGTTGTAAACCATGTTCTCTGGCTTACTCCAGCTATCGCCATGTTTAGCGATGACATGATGAGGAACCGGGAACAGCGTCGGCCAGGCGAGCATCGTCGTGAACCAAGGAACCGGCTGCTCAAGCGTAACTTCAAGAGTACGATCATCCGTCGCTTTGACACCCAGCGATGTAACATCTGCTTTACCCGCAACAATAGCTGCGGCATTGTTAATGTGCGCCTGTGCCAGATAGCCAGCAAAAGGGCTTGCCGTTTTCGGGTCAACCGCGCGCTGCCAGCCGAGGACAAAATCC  >  NC_000913/3166913‑3167383                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                            gcCGGAACCCAGGTGAGATCGACCTCTCCCGCGCGATAGCGGTTATAGCCGGTGACCGAATTATCTAGCGCCAGATACTCAACCTGTTGCAATACTGTATGTTGCGCATCGCGGTACTTTGGATTTTTGCGTGCAGTAATCTTTTCGTTAACTACCCACTGATCAAGCACAAAGGCACCGTTGTAAACCATGTTCTCTGGCTTACTCCAGCTATCGCCATGTTTAGCGATGACATAATGAGGAACCGGGa                                                                                                                                                                                                                               >  1:153349/1‑250 (MQ=255)    ggAACCCAGGTGAGATCGACCTCTCCCGCGCGATAGCGGTTATAGCCGGTGACCGAATTATCTAGCGCCAGATACTCAACCTGTTGCAATACTGTATGTTGCGCATCGCGGTACTTTGGATTTTTGCGTGCAGTAATCTTTTCGTTAACTACCCACTGATCAAGCACAAAGGCACCGTTGTAAACCATGTTCTCTGGCTTACTCCAGCTATCGCCATGTTTAGCGATGACATAATGAGGAACCGGGAAc                                                                                                                                                                                                                             >  1:20196/1‑249 (MQ=255)        ccccggTGAGATCGACCTCTCCCGCGCGATAGCGGTTATAGCCGGTGACCGAATTATCTAGCGCCAGATACTCAACCTGTTGCAATACTGTATGTTGCGCATCGCGGTACTTTGGATTTTGGCGTGCAGTAATCTTTTCGTTAACTACCCACTGATCAAGCACAAAGGCACCGTTGTAAACCATGTTCTCTGGCTTACTCCAGCTATCGCCATGTTTAGCGATGACATAATGAGGAACCGGGAACAGCGTc                                                                                                                                                                                                                       <  2:142148/247‑1 (MQ=255)         ccAGGTGAGATCGACCTCTCCCGCGCGATAGCGGTTATAGCCGGTGACCGAATTATCTAGCGCCAGATACTCAACCTGTTGCAATACTGTATGTTGCGCATCGCGGTACTTTGGATTTTTGCGTGCAGTAATCTTTTCGTTAACTACCCACTGATCAAGCACAAAGGCACCGTTGTAAACCATGTTCTCTGGCTTACTCCAGCTATCGCCATGTTTAGCGATGACATAATGAGGAACCGGGAACAGCGTCg                                                                                                                                                                                                                      <  2:104003/251‑1 (MQ=255)           aGGTGAGATCGACCTCTCCCGCGCGATAGCGGTTATAGCCGGTGACCGAATTATCTAGCGCCAGATACTCAACCTGTTGCAATACTGTATGTTGCGCATCGCGGTACTTTGGATTTTTGCGTGCAGTAATCTTTTCGTTAACTACCCACTGATCAAGCACAAAGGCACCGTTGTAAACCATGTTCTCTGGCTTACTCCAGCTATCGCCATGTTTAGCGATGACATAATGAGGAACCGGGAACAGCGTCGGc                                                                                                                                                                                                                    <  2:103413/251‑1 (MQ=255)                      aCCTCTCCCGCGAGATAGCGGTTATAGCCGGTGACCGAATTATCTAGCGCCAGATACTCAACCTGTTGCAATACTGTATGTTGCGCATCGCGGTACTTTGGATTTTTGCGTGCAGTAATCTTTTCGTTAACTACCCACTGATCAAGCACAAAGGCACCGTTGTAAACCATGTTCTCTGGCTTACTCCAGCTATCGCCATGTTTAGCGATGACATAATGAGGAACCGGGAACAGCGTCGGCCAGGCGAGCAt                                                                                                                                                                                                         >  2:155789/1‑251 (MQ=255)                                 gcgATAGCGGTTATAGCCGGTGACCGAATTATCTAGCGCCAGATACTCAACCTGTTGCAATACTGTATGTTGCGCATCGCGGTACTTTGGATTTTTGCGTGCAGTAATCTTTTCGTTAACTACCCACTGATCAAGCACAAAGGCACCGTTGTAAACCATGTTCTCTGGCTTACTCCAGCTATCGCCATGTTTAGCGATGACATAATGAGGAACCGGGAACAGCGTCGGCCAGGCGAGCATCGTCGTGAAcc                                                                                                                                                                                              >  1:184250/1‑251 (MQ=255)                                  cgATAGCGGTTATAGCCGGTGACCGAATTATCTAGCGCCAGATACTCAACCTGTTGCAATACTGTATGTTGCGCATCGCGGTACTTTGGATTTTTGCGTGCAGTAATCTTTTCGTTAACTACCCACTGATCAAGCACAAAGGCACCGTTGTAAACCATGTTCTCTGGCTTACTCCAGCTATCGCCATGTTTAGCGATGACATAATGAGGAACCGGGAACAGCGTCGGCCAGGCGAGCATCGTCGTGAACCa                                                                                                                                                                                             <  1:286556/251‑1 (MQ=255)                                    aTAGCGGTTATAGCCGGTGACCGAATTATCTAGCGCCAGATACTCAACCTGTTGCAATACTGTATGTTGCGCATCGCGGTACTTTGGATTTTTGCGTGCAGTAATCTTTTCGTTAACTACCCACTGATCAAGCACAAAGGCACCGTTGTAAACCATGTTCTCTGGCTTACTCCAGCTATCGCCATGTTTAGCGATGACATAATGAGGAACCGGGAACAGCGTCGGCCAGGCGAGCATCGTCGTGAACCa                                                                                                                                                                                             >  2:93662/1‑249 (MQ=255)                                                      gACCGAATTATCTAGCGCCAGATACTCAACCTGTTGCAATACTGTATGTTGCGCATCGCGGTACTTTGGATTTTTGCGTGCAGTAATCTTTTCGTTAACTACCCACTGATCAAGCACAAAGGCACCGTTGTAAACCATGTTCTCTGGCTTACTCCAGCTATCGCCATGTTTAGCGATGACATAATGAGGAACCGGGAACAGCGTCGGCCAGGCGAGCATCGTCGTGAACCAAGGAACCGGCTGCTCAAGc                                                                                                                                                                          <  2:20196/250‑1 (MQ=255)                                                               aTCTAGCGCCAGATACTCAACCTGTTGCAATACTGTATGTTGCGCATCGCGGTACTTTGGATTTTTGCGTGCAGTAATCTTTTCGTTAACTACCCACTGATCAAGCACAAAGGCACCGTTGTAAACCATGTTCTCTGGCTTACTCCAGCTATCGCCATGTTTAGCGATGACATAATGAGGAACCGGGAACAGCGTCGGCCAGGCGAGCATCGTCGTGAACCAAGGAACCGGCTGCTCAAGCGTAACTTCa                                                                                                                                                                 >  1:78480/1‑250 (MQ=255)                                                                                  aCCTGTTGCAATACTGTATGTTGCGCATCGCGGTACTTTGGATTTTTGCGTGCAGTAATCTTTTCGTTAACTACCCACTGATCAAGCACAAAGGCACCGTTGTAAACCATGTTCTCTGGCTTACTCCAGCTATCGCCATGTTTAGCGATGACATAATGAGGAACCGGGAACAGCGTCGGCCAGGCGAGCATCGTCGTGAACCAAGGAACCGGCTGCTCAAGCGTAACTTCAAGAGTACGATCATCCGTCGc                                                                                                                                             >  1:262725/1‑251 (MQ=255)                                                                                       ttGCAATACTGTATGTTGCGCATCGCGGTACTTTGGATTTTTGCGTGCAGTAATCTTTTCGTTAACTACCCACTGATCAAGCACAAAGGCACCGTTGTAAACCATGTTCTCTGGCTTACTCCAGCTATCGCCATGTTTAGCGATGACATAATGAGGAACCGGGAGCAGCGTCGGCCAGGCGAGCATCGTCGTGAACCAAGGAACCGGCTGCTCAAGCGTAACTTCAAGAGTACGATCATCCGTCGCTTTg                                                                                                                                         >  1:171592/1‑250 (MQ=255)                                                                                                           cATCGCGGTACTTTGGATTTTTGCGTGCAGTAATCTTTTCGTTAACTACCCACTGATCAAGCACAAAGGCACCGTTGTAAACCATGTTCTCTGGCTTACTCCAGCTATCGCCATGTTTAGCGATGACATAATGAGGAACCGGGAACAGCGTCGGCCAGGCGAGCATCGTCGTGAACCAAGGAACCGGCTGCTCAAGCGTAACTTCAAGAGTACGATCATCCGTCGCTTTGACACCCAGCGATGTAACATCt                                                                                                                    <  1:244286/251‑1 (MQ=255)                                                                                                                                  cGTGCAGTAATCTTTTCGTTAACTACCCACTGATCAAGCACAAAGGCACCGTTGTAAACCATGTTCTCTGGCTTACTCCAGCTATCGCCATGTTTAGCGATGACATAATGAGGAACCGGGAACAGCGTCGGCCAGGCGAGCATCGTCGTGAACCAAGGAACCGGCTGCTCAAGCGTAACTTCAAGAGTACGATCATCCGTCGCTTTGACACCCAGCGATGTAACATCTGCTTTACCCg                                                                                                          >  1:121597/1‑238 (MQ=255)                                                                                                                                  cGTGCAGTAATCTTTTCGTTAACTACCCACTGATCAAGCACAAAGGCACCGTTGTAAACCATGTTCTCTGGCTTACTCCAGCTATCGCCATGTTTAGCGATGACATAATGAGGAACCGGGAACAGCGTCGGCCAGGCGAGCATCGTCGTGAACCAAGGAACCGGCTGCTCAAGCGTAACTTCAAGAGTACGATCATCCGTCGCTTTGACACCCAGCGATGTAACATCTGCTTTACCCg                                                                                                          <  2:121597/238‑1 (MQ=255)                                                                                                                                               tttCGTTAACTACCCACTGATCAAGCACAAAGGCACCGTTGTAAACCATGTTCTCTGGCTTACTCCAGCTATCGCCATGTTTAGCGATGACATAATGAGGAACCGGGAACAGCGTCGGCCAGGCGAGCATCGTCGTGAACCAAGGAACCGGCTGCTCAAGCGTAACTTCAAGAGTACGATCATCCGTCGCTTTGACACCCAGCGATGTAACATCTGCTTTACCCGCAACAATAGCTGCGGCATTGtt                                                                                    <  1:182125/247‑1 (MQ=255)                                                                                                                                                     tAACTACCCACTGATCAAGCACAAAGGCACCGTTGTAAACCATGTTCTCTGGCTTACTCCAGCTATCGCCATGTTTAGCGATGACATAATGAGGAACCGGGAACAGCGTCGGCCAGGCGAGCATCGTCGTGAACCAAGGAACCGGCTGCTCAATCGTAACTTCAAGAGTACGATCATCCGTCGCTTTGACACCCAGCGATGTAACATCTGCTTTACCCGCAACAATAGCTGCGGCATTTTAAATGTGCGcc                                                                          >  1:158367/1‑251 (MQ=255)                                                                                                                                                        cTACCCACTGATCAAGCACAAAGGCACCGTTGTAAACCATGTTCTCTGGCTTACTCCAGCTATCGCCATGTTTAGCGATGACATAATGAGGAACCGGGAACAGCGTCGGCCAGGCGAGCATCGTCGTGAACCAAGGAACCGGCTGCTCAAGCGTAACTTCAAGAGAACGATCATCCGTCGCTTTGACACCCAGCGATGTAACATCTGCTTTACCCGCAACAATAGCTGCGGCATTGTTAATGTGCGCCttt                                                                       >  2:127872/1‑249 (MQ=255)                                                                                                                                                                 gATCAAGCACAAAGGCACCGTTGTAAACCATGTTCTCTGGCTTACTCCAGCTATCGCCATGTTTAGCGATGACATAATGAGGAACCGGGAACAGCGTCGGCCAGGCGAGCATCGTCGTGAACCAAGGAACCgg                                                                                                                                                                                    >  1:26784/1‑133 (MQ=255)                                                                                                                                                                 gATCAAGCACAAAGGCACCGTTGTAAACCATGTTCTCTGGCTTACTCCAGCTATCGCCATGTTTAGCGATGACATAATGAGGAACCGGGAACAGCGTCGGCCAGGCGAGCATCGTCGTGAACCAAGGAACCgg                                                                                                                                                                                    <  2:26784/133‑1 (MQ=255)                                                                                                                                                                   tCAAGCACAAAGGCACCGTTGTAAACCATGTTCTCTGGCTTACTCCAGCTATCGCCATGTTTAGCGATGACATAATGAGGAACCGGGAACAGCGTCGGCCAGGCGAGCATCGTCGTGAACCAAGGAACCGGCTGCTCAAGCGTAACTTCAAGAGTACGATCATCCGTCGCTTTGACACCCAGCGATGTAACATCTGCTTTACCCGCAACAATAGCTGCGGCATTGTTAATGTGCGCCTGTGCCAGATAGcc                                                            >  2:320069/1‑251 (MQ=255)                                                                                                                                                                        cacaAAGGCACCGTTGTAAACCATGTTCTCTGACTTACTCCAGCTATCGCCATGTTTAGCGATGACATAATGAGGAACCGGGAACAGCGTCGGCCAGGCGAGCATAGTCGTGAACCAAGGAACCGGCTGCTCAAGCGTAACTTCAAGCGTACGATCATCCGTCGCTTTGACACCCAGCGATGTAACATCTGCTTTACCCGCAACAATAGCTGCGGCATTGTTAATGTGCGCCTGTTCCAGATAGCCAGCa                                                        >  1:192553/1‑250 (MQ=255)                                                                                                                                                                         acaAAGGCACCGTTGTAAACCATTTTCTCTGGCTTACTCCAGCTATCGCCATGTTTAGCGATGACATAATGAGGAACCGGGAACAGCGTCGTCCAGGCGAGCATCGTCGTGAACCAAGGAACCGGCTGCTCAAGCGTAACTTCAAGAGTACTATCATCCGTCGCTTTGACACCCAGCGATGTAACATCTGCTTTACCCGCAACAATAGCTGCGGCATTGTTAATGTGCGCCTGTGCCAGATAGCCAGCaaa                                                      <  1:326814/251‑1 (MQ=255)                                                                                                                                                                         acaAAGGCACCGTTGTAAACCATGTTCTCTGGCTTACTCCAGCTATCGCCATGTTTAGCGATGACATAATGAGGAACCGGGAACAGCGTCGGCCAGGCGAGCATCGTCGTGAACCAAGGAACCGGCTGCTCAAGCGTAACTTCAAGAGTACGATCATCCGTCGCTTTGACACCCAGCGATGTAACATCTGCTTTACCCGCAACAATAGCTGCGGCATTGTTAATGTGCGCCTGTGCCAGATAGCCAGCaa                                                       >  2:81662/1‑250 (MQ=255)                                                                                                                                                                             aGGCACCGTTGTAAACCATGTTCTCTGGCTTACTCCAGCTATCGCCAGGTTTAGCGATGACATAATGAGGAACCGGGAACAGCGTCGGCCAGGCGAGCATCGTCGTGAACCAAGGAACCGGCTGCTCAAGCGTAACTTCAAGAGTACGAGCATCCGTCGCTTTGACACCCAGCGATGTAACATCTGCTTTACCCGCAACAATAGCTGCGGCAGTGTTAATGTGCGCCTGTGCCAGATAGCCAGCAAAAggt                                                  <  2:306270/251‑2 (MQ=255)                                                                                                                                                                                    gTTGTAAACCATGTTCTCTGGCTTACTCCAGCTATCGCCATGTTTAGCGATTACATAATGAGGAACCGGGAACAGCGTCGGCCAGGCGAGCATCGTCGTGAACCAAGGAACCGGCTGCTCAAGCGTAACTTCAAGAGTACGATCATCCGTCGCTTTGACACCCAGCGATGTAACATCTGCTTTACCCGCAACAATAGCTGCGGCATTGTTAATGTGCGCCTGTGCCAGATAGCCAGCAAAAGGGCTTGCCg                                           <  1:190134/251‑1 (MQ=255)                                                                                                                                                                                                       ggCTTACTCCAGCTATCGCCATGTTTAGCGATGACATAATGAGGACCCGGGAACAGCGTCGGCCAGGCGAGCATCGTCGTGAACCAAGGAACCGGCTGCTCAAGCGTAAATTCAAGCGTACGATCATCCGTCGCTTTGACACCCAGCGATGTAACATCTGCTTTACCCGCAACAATAGCTGCGGCATTGTTAATGTGCGCCTGTGCCAGATAGCAAGCAAAAGGGCTTGCCGTTTTCGGGTCAACcgagcg                        >  1:192460/1‑251 (MQ=255)                                                                                                                                                                                                        gCTTACTCCAGCTATCGCCATGTTTAGCGATGACATAATGAGGAACCGGGAACAGCGTCGGCCAGGCGAGCATCGTCGTGAACCAAGGAACCGGCTGCTCAAGCGTAACTTCAAGAGTACGATCATCCGTCGCTTTGACACCCAGCGATGTAACATCTGCTTTACCCGCAACAATAGCTGCGGCATTGTTAATGTGCGCCTGTGCCAGATAGCCAGCAAAAGGGCTTGCCGTTTTCGGGTCAACCgcgcgc                       >  1:88822/1‑251 (MQ=255)                                                                                                                                                                                                                             gTTTAGCGATGACATAATGAGGAACCGGGAACAGCGTCGGCCAGGCGAGCATCGTCGTGAACCAAGGAACCGGCTGCTCAAGCGTAACTTCAAGAGTACGATCATCCGTCGCTTTGACACCCAGCGATGTAACATCTGCTTTACCCGCAACCATAGCTGCGGCATTGTTAATGTGCGCCTGTGCCAGATAGCCAGCAAAAGGGCTTGCCGTTTTCGGGTCAACCGCGCGCTGCCAGCCGCGGCCAAAATcc  >  2:278407/1‑251 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                            GCCGGAACCCAGGTGAGATCGACCTCTCCCGCGCGATAGCGGTTATAGCCGGTGACCGAATTATCTAGCGCCAGATACTCAACCTGTTGCAATACTGTATGTTGCGCATCGCGGTACTTTGGATTTTTGCGTGCAGTAATCTTTTCGTTAACTACCCACTGATCAAGCACAAAGGCACCGTTGTAAACCATGTTCTCTGGCTTACTCCAGCTATCGCCATGTTTAGCGATGACATGATGAGGAACCGGGAACAGCGTCGGCCAGGCGAGCATCGTCGTGAACCAAGGAACCGGCTGCTCAAGCGTAACTTCAAGAGTACGATCATCCGTCGCTTTGACACCCAGCGATGTAACATCTGCTTTACCCGCAACAATAGCTGCGGCATTGTTAATGTGCGCCTGTGCCAGATAGCCAGCAAAAGGGCTTGCCGTTTTCGGGTCAACCGCGCGCTGCCAGCCGAGGACAAAATCC  >  NC_000913/3166913‑3167383

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑