Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	922,954	G→T	A14A (GCG→GCT)	clpS →	specificity factor for ClpA‑ClpP chaperone‑protease complex

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	922,954	0	G	T	100.0%	54.2 / NA	17	A14A (GCG→GCT)	clpS	specificity factor for ClpA‑ClpP chaperone‑protease complex
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (9/8);  total (9/8)

GGACAAGGGGCAGGTATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACAGGAATGTGACAGATGTCGCTGATGCCAACGATAGATGATAGTTATCTATCATGTGGAGTAGATTGGTCAGGCAAATAAGCTCTTGTCAGCGGCAGGGCGTTCTGCCGATAACCGTAACCGAAGATGATAACTGACAATGGGTAAAACGAACGACTGGCTGGACTTTGATCAACTGGCGGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTATGTATAAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAATTCTTTTCTTATGATGTAGAACGTGCAACGCAATTGATGCTCGCTGTTCACTACCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGTGAACAAGTACGCGAGGGAGAATG  >  NC_000913/922725‑923198                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     ggACAAGGGGCAGGTATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACAGGAATGTGACAGATGTCGCTGATGCCAACGATAGATGATAGTTATCTATCATGTGGAGTAGATTGGTCAGGCAAATAAGCTCTTGTCAGCGGCAGGGCGTTCTGCCGATAACCGTAACCGAAGATGATAACTGACAATGGGTAAAACGAACGACTGGCTGGACTTTGATCAACTGGCTGAAGAAAAAGTTCGCGAcgcg                                                                                                                                                                                                                                 <  1:149488/251‑1 (MQ=255)                   aaTCAAAAATTTGAAGCAGTTAAAGCTATTGACAGGAATGTGACCGATGTCGCTGATGCCAACGATAGATGCTAGTAATCTATCATGTGGAGTAGATTGGTCAGGAAAATAAGCTCTTGTAAGCGGCAGGGCGTTATGCAGATAACCGTAACCGAAGATGATAACTGACAATGGGTAAAACGAACGACTGGCTGGACTTTGATCAACTGACTGCAGAAAAAGATCGCGACGCGATACAACCGCCATCTATg                                                                                                                                                                                                               >  1:188961/1‑251 (MQ=255)                       aaaaaTTTGAAGCAGTTAACGCTATTGACAGGAATGTGACAGATGTCGCTGATGCCAACGATAGATGATAGTTATCTATCATGTGGAGTAGATTGGTCAGGCAAATAAGCTCTTGTCAGCGGCAGGGCGTTCTGCCGATAACCGTAACCGAAGATGATAACTGACAATGGGTAAAACGAACGACTGGCTGGACTTTGATCAACTGGCTGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTATGtat                                                                                                                                                                                                            >  2:88949/1‑250 (MQ=255)                                              aTTGACAGGAATGTGACAGATGTCGCTGATGCCAACGATAGATGATAGTTATCTATCATGTGGAGTAGATTGGTCAGGCAAATAAGCTCTTGTCAGCGGCAGGGCGTTCTGCCGATAACCGTAACCGAAGATGATAACTGACAATGGGTAAAACGAACGACTGGCTGGACTTTGATCAACTGGCTGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCGATGTATAAAGTGATATTAGTCAATgatgat                                                                                                                                                                                    <  1:246194/251‑1 (MQ=255)                                                          gtgACAGATGTCGCTGATGCCAACGATAGATGATAGTTATCTATCATGTGGAGTAGATTGGTCAGGCAAATAAGCTCTTGTCAGCGGCAGGGCGTTCTGCCGATAACCGTAACCGAAGATGATAACTGACAATGGGTAAAACGAACGACTGGCTGGACTTTGATCAACTGGCTGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTATGTATAAAGTGATATTAGTCAATGATGATTACACTCCGAt                                                                                                                                                                         <  1:211210/250‑1 (MQ=255)                                                                          aTGCCAACGATAGCTGATAGTTATCTATCATGTGGAGTAGATTGGTCAGGCAAATAAGCTCTTGTCAGCGGCAGGGCGTTCTGCCGATAACCGTAACCGAAGATGATAACTGACAATGGGTAAAACGAACGACTGGCTGGACTTTGATCAACTGGCTGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTATGTATAAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGTTATTGACg                                                                                                                                                        >  2:145510/1‑251 (MQ=255)                                                                                   aTAGATGATAGTTATCTATCATGTGGAGTAGATTGGTCAGGCAAATAAGCTCTTGTCAGCGGCAGGGCGTTCTGCCGATAACCGTAACCGAAGATGATAACTGACAATGGGTAAAACGAACGACTGGCTGGACTTTGATCAACTGGCTGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTATGTATAAAGTGATATTAGTCa                                                                                                                                                                                            >  1:66797/1‑206 (MQ=255)                                                                                   aTAGATGATAGTTATCTATCATGTGGAGTAGATTGGTCAGGCAAATAAGCTCTTGTCAGCGGCAGGGCGTTCTGCCGATAACCGTAACCGAAGATGATAACTGACAATGGGTAAAACGAACGACTGGCTGGACTTTGATCAACTGGCTGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTATGTATAAAGTGATATTAGTCa                                                                                                                                                                                            <  2:66797/206‑1 (MQ=255)                                                                                                      cATGTGGAGTAGATTGGTCAGGCCAATAAGCTCTTGTCAGCGGCAGGGCGTTCTGCCGATAACCGTAACCGAAGATGATAACTGACAATGGGTAAAACGAACGACTGGCTGGACTTTGATCAACTGGCTGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTATGTATAAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAATTCTTTTCTTATGATGt                                                                                                                            >  1:66924/1‑251 (MQ=255)                                                                                                              gTAGATTGGTCAGGCAAATAAGCTCTTGTCAGCGGCAGGGGGTTCTGCCGATAACCGTAACCGAAGAAGATAACTGACAATGGGTAAAACGAACGACTGGCTTGACTTTGATCAACTGGCTGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTATGTATAAAGTGATATTAGTCAATGATGATTACACTCCTATGGAGTGTGTTATTGACGTGTTACAAAAATTCTTGTCTTATGATGTAGAACGTg                                                                                                                    <  2:315253/251‑1 (MQ=255)                                                                                                                        cAGGCAAATAAGCTCTTGTCAGCGGCAGGGCGTTCTGCCGATAACCGTAACCGAAGATGATAACTGACAATGGGTAAAACGAACGACTGGCTGGACTTTGATCAACTGGCTGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTATGTATAAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAATTCTTTTCTTATGATGTAGAACGTGCAACGCAAt                                                                                                           <  1:293860/250‑1 (MQ=255)                                                                                                                                         gTCAGCGGCAGGGCGTTCTGCCGATAACCGTAACCGAAGATGATAACTGACAATGGGTAAAACGAACGACTGGCTGGACTTTGATCAACTGGCTGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTATGTATAAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAATTCTTTTCTTATGATGTAGAACGTGCAACGCAATTGATGCTCGCTGTTCACt                                                                                         <  1:88949/251‑1 (MQ=255)                                                                                                                                                                            gAAGATGATAACTGACAATGGGTAAAACGAACGACTGGCTGGACTTTGATCAACTGGCTGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTATGTATAAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAATTCTTTTCTTATGATGTAGAACGTGCAACGCAATTGATGCTCGCTGTTCACTACCAGGGGAAGGCCATTTGCGGAGTCTTTAccgcc                                                      <  1:145510/251‑1 (MQ=255)                                                                                                                                                                                     aaatGACAATAGGAAAAACGACCGAATGGCTGGACTTTGATCAACCGGATGACGAACAAGCTCGCGACACGCTAAAACCGACATCTATGTATAAAGAGATATTAGTCACTGATGATTCCAATCCGATGGAGTTTGTTATTGACGTGTTACAAAAATTCTTTTCTTATGATGTAGAACGTGCAACGCAATTGATGATCGCTGTTCAATAACAGGGGACGGCCATTGGAGGAGTCTTTACCGCCGAGGTTGc                                              >  2:312037/4‑250 (MQ=255)                                                                                                                                                                                           cAATGGGTAAAACGAACGACTGGCTGGACTTTGATCAACTGGCTGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTATGTATAAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAATTCTTTTCTTATGATGTAGAACGTGCAACGCAATTGATGCTCGCTGTTCACTACCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAAcc                                       >  2:157073/1‑251 (MQ=255)                                                                                                                                                                                                      acgaacgaCTGGCTGGACTTTGATCAACTGGCTGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTATGTATAAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAATTCTTTTCTTATGATGTAGAACGt                                                                                                                     >  1:79915/1‑162 (MQ=255)                                                                                                                                                                                                                                aCTGGCTGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTATGTATAAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAATTCTTTTCTTATGATGTAGAACGTGCAACGCAATTGATGCTCGCTGTTCACTACCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGTGAACAAGTACGCGAGGGAGAATg  >  1:268388/1‑251 (MQ=255)                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     GGACAAGGGGCAGGTATGAATCAAAAATTTGAAGCAGTTAACGCTATTGACAGGAATGTGACAGATGTCGCTGATGCCAACGATAGATGATAGTTATCTATCATGTGGAGTAGATTGGTCAGGCAAATAAGCTCTTGTCAGCGGCAGGGCGTTCTGCCGATAACCGTAACCGAAGATGATAACTGACAATGGGTAAAACGAACGACTGGCTGGACTTTGATCAACTGGCGGAAGAAAAAGTTCGCGACGCGCTAAAACCGCCATCTATGTATAAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAATTCTTTTCTTATGATGTAGAACGTGCAACGCAATTGATGCTCGCTGTTCACTACCAGGGGAAGGCCATTTGCGGAGTCTTTACCGCCGAGGTTGCAGAAACCAAAGTGGCGATGGTGAACAAGTACGCGAGGGAGAATG  >  NC_000913/922725‑923198

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑