Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,253,002	C→T	intergenic (+17/+83)	dhaR → / ← ycgV	DNA‑binding transcriptional dual regulator DhaR/putative autotransporter adhesin YcgV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,253,002	0	C	T	100.0%	38.1 / NA	14	intergenic (+17/+83)	dhaR/ycgV	DNA‑binding transcriptional dual regulator DhaR/putative autotransporter adhesin YcgV
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/7);  total (7/7)

GCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTCCACCAGTCTGTCATTTGCGGAAGTTGAAAAAGAGGCAATTATTAACGCAGCCCAGGTCACAGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCAGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAA‑TTTTCAGCATCGTTTAAGGGCTTGTCTATCCCGCACTTAAAAGCTGAAGCGATATCCTCCGTTGACTTGCTTCTGATCAAAGAGGTTACCCTGCGTGTAATCCGCTTCGAGATAGAATGTGTGCTGTTTGTTATACTGTGCACTGACTCCCACGCCGTTATTCCAGCCATTACCTTTGAAACTGT  >  NC_000913/1252778‑1253233                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                         gcatgctaCAGATGATGTCAGCGCCACCCGCCTTTCCACCAGTCTGTCATTTGCGGAAGTTGAAAAAGAGGCAATTATTAACGCAGCCCAGGTCACAGGCGGTCGCAGTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCAGTGATGCAGGGCCGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTTTGATACATCAGAGTGATCTGTATTTc                                                                                                                                                                                                                <  1:168876/244‑1 (MQ=255)                         cACCCGCCTTTCCACCAGTCTGTCATTTGCGGAAGTTGAAAAAGAGGCAATTATTAACGCAGCCCAGGTCACAGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCAGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTTTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAA‑tttt                                                                                                                                                                                       <  1:137336/251‑1 (MQ=255)                                     caccaGTCTGTCATTTGCGGAAGTTGAAAAAGAGGCAATTATTAACGCAGCCCAGGTCACAGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCAGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTTTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAA‑TTTTCAGCATCGTTTa                                                                                                                                                                           <  1:49186/251‑1 (MQ=255)                                       ccaGTCTGTCCTTTGCGGCAGTTGAAAAAGAGGCAATTATTAACGCAGCCCAGGTCACAGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTTGGGTCGGCCGCACTACGCTGGGGCGGGAAATGAAGCAACATGGCATTTATGCAGGGCCGGTTAAGCGCCGGGGATGAAAGACAGAAACGATTTTTTATACATCAGAGAGATCTGTTTTTCATTTCGGGGCACGCTAAAAAATTTTC‑GCATCggttta                                                                                                                                                                          >  2:55583/1‑245 (MQ=255)                                                                                                             tCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCAGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTTTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAA‑TTTTCAGCATCGTTTAAGGGCTTGTCTATCCCGCACTTAAAAGCTGAAGCGATATCCTCCGTTGACTTGCTTCTGATCAAAGAGGtt                                                                                                    <  2:245206/250‑1 (MQ=255)                                                                                                                                                      tgtgGCGGAAAAAGAAGAAAACCGGCCTTGATGCAGGGCAGGTTAAGCGCCGGGCATGAAAGACAGCAACGATTTTTGATAAATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAA‑TTTTCAGCATCGTTTAAGGGCTTGTCTATCCCGCACTTAAAAGCTGAAGCGATATCCTCCGTTGCCTTGCTTCTGATCAAAAAGGTTACCCTGCGTGTACTCCGCTTCGCGCTTGAATGTGTTCtgttt                                                          >  2:230344/1‑251 (MQ=255)                                                                                                                                                                  tGAAGCAACATGGCATTGATGCAGGGCAGTTTAAGCGCCGGGTATGAACGACAGAAACGATTTTTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAA‑TTTTCAGCATCGTTTAAGGGCTTGTCTATCCCGCACTTAAAAGCTGAAGCGATATCCTCCGTTGACTTGCTTCTGATCAAAGAGGTTACCCTGCGTGTAATCCGCTTCGAGATAGAATGTGTGCTGTTTGTTATACTGTGc                                              >  2:30004/1‑251 (MQ=255)                                                                                                                                                                   gAAGCAACAAGGCATTGATGCAGGCCAGATTAAGAGCCGGGTATGAAAGACAGAAACGATTTTTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAA‑TTTTCAGCATCGTTTAAGGGCTTGTCTATCCAGCACTTACAAGCTGAAGCGATATCCTCCGTTGACTTGCTTCTGATCAAAGAGGTTACCCTGCGTGTAATCAGCTTCGAGAGAGAATGTGTGCTGTTTGTTATACTGTGc                                              >  2:223181/1‑250 (MQ=255)                                                                                                                                                                                  tGATGCAGGGCCGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTTTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAA‑TTTTCAGCATCGTTTAAGGGCTTGTCTATCCCGCACTTAAAAGCTGAAGCGATATCCTCCGTTGACTTGCTTCTGATCAAAGAGGTTACCCTGCGTGTAATCCGCTTCGAGATAGAATGTGTGCTGTTTGTTATACTGTGCACTGACTCACACGCCg                              >  2:168023/1‑251 (MQ=255)                                                                                                                                                                                            cAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTTTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAA‑TTTTCAGCATCGTTTAAGGGCTTGTCTATCCCGCACTTAAAAGCTGAAGCGATATCCTCCGTTGACTTGCTTCTGATCAAAGAGGTTACCCTGCGTGTAATCCGCTTCGAGATAGAATGTGTGCTGTTTGTTATACTGTGCACTGACTCCCACGCCGTTATTCCAGc                    >  2:273103/1‑251 (MQ=255)                                                                                                                                                                                               tttAAGCGCCGGGTATGAAAGACAGAAACGATTTTTGATACATCCGAGTGATCTGTATTTCAATCCGGCGCACGCTAACAA‑TTTTCAGCATCGATTAAGGGCTTGTCTATCCCGCACTTAAAAGCTGACGCGCTATCCTCAGTTGACTTGTTTCTGATCAAAGAGGTTACCCTGCGTGTAATCCGCTTCGCGATAGCATGTGTGCTGTTTGTTAGACAGTGCACTGACAGCACCGCCGTTATTCCAGCCAt                 >  2:226654/1‑251 (MQ=255)                                                                                                                                                                                                  aaGCGCCGGGTATGAAAGACAGAAACGATTTTTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAA‑TTTTCAGCATCGTTTAAGGGCTTGTCTATCCCGCACTTAAAAGCTGAAGCGATATCCTCCGTTGACTTGCTTCTGATCAAAGAGGTTACCCTGCGTGTAATCCGCTTCGAGATAGAATGGGTGCTGTTTGTTATACTGTGCACTGACTCCCACGCCGTTATTCCAGCCATTAc              <  1:192854/251‑1 (MQ=255)                                                                                                                                                                                                      cccGGGTATGAAAGACAGAAACGATTTTTGATACATCAGAGTGATCTGTATTTCATGCCGGCGCCCGCTAACAA‑TTTTCATCATCGTTAAAGGGCTTGTCTATCCCGCACTTAAAAGCTGAAGCGATATCCTCCGTTGACTTGCTTCTGATCAAAGAGGTCTCCCTGGTTGTAATCCGCTTCGAAATAGAATGTGTGCTGTGTGTTATACTGTGCACTGACTCCCACGCCGTTATTCCAGCCATTACCttt          <  1:132161/250‑1 (MQ=255)                                                                                                                                                                                                               gAAAGACAGAAACGATTTTTGATACATCGGAGTGATCTGTATTTCATTCCGGGGCACGCTAACAA‑TTTTCAGCATCGTTTAAGGCCTTGTCTATCCCGCACTTAAAAGTGGAAGCGATATCCTCCGTTTACTTGCTTCTGATCAAAGGGGTTACCCTGCGTGTAATCCGCTTCGAGATAGAATGTGTGCTGTTTGTTATACTGTGCACTGACTCCCACGCCGTTATTCCAGCCATTACCTTTGAAACTGt  <  2:145381/250‑1 (MQ=255)                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                         GCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTCCACCAGTCTGTCATTTGCGGAAGTTGAAAAAGAGGCAATTATTAACGCAGCCCAGGTCACAGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCAGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAA‑TTTTCAGCATCGTTTAAGGGCTTGTCTATCCCGCACTTAAAAGCTGAAGCGATATCCTCCGTTGACTTGCTTCTGATCAAAGAGGTTACCCTGCGTGTAATCCGCTTCGAGATAGAATGTGTGCTGTTTGTTATACTGTGCACTGACTCCCACGCCGTTATTCCAGCCATTACCTTTGAAACTGT  >  NC_000913/1252778‑1253233

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑