Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,645	G→A	W282* (TGG→TAG)	thrB →	homoserine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,645	0	G	A	100.0%	89.8 / NA	29	W282* (TGG→TAG)	thrB	homoserine kinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/21);  total (8/21)

GGCGACATCTGGCAGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAGTACTGGAAAACTAAATGAAACTCTACAATCTGAAAGATCACAACGAGCAGGTCAGCTTTGCGCA‑AGCCGTAACCCAGGGGTTGGGCAAAAATCA‑GGGGCTGTTTTTTCCGCACGACCTGCCGGAATTCAGCCTGACTGAAATTGATGAGATGCTGAAGCTGGATTT  >  NC_000913/3411‑3885                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                   ggCGACATCTGGCAGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTAGTTGGGGAAGAACTAc                                                                                                                                                                                                                                    >  1:255948/1‑251 (MQ=255)           ggCAGGCTTCATTCACGCCTTCTATTCCCGTCAGCCTGAGCTTGCCGCTAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCTACTAGTTGGGTAAGAACTACCTGCAAAAt                                                                                                                                                                                                                           <  1:100067/250‑1 (MQ=255)           ggCAGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGCAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTAGTTGGGTAAGaa                                                                                                                                                                                                                                        >  1:196449/1‑237 (MQ=255)           ggCAGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCAGGCGTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCGTGTTCGCGCTGTGTGACAAGCCGGAAACCGCCCAGCGCGGTGCCGACTAGTTTTGTAAGaa                                                                                                                                                                                                                                        <  2:196449/237‑1 (MQ=255)             cAGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGGTATCGCTGAACCCTACCGTGAACGGTTACTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTAGTTGGGTAAGAACTACCTGCAAAATCAg                                                                                                                                                                                                                        <  1:249190/251‑1 (MQ=255)              aGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGGATCTCCGGCCCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCCGCGCGTTGCCGACTAGTTGGGTAGGAACTACCTGCCAAAtacgg                                                                                                                                                                                                                       >  2:137219/1‑247 (MQ=255)                   tcattcaCGCCTGCGATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCTCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTATTTGGGTAAGAACTACCTGCAAAATCAGGaatg                                                                                                                                                                                                                   <  2:238849/250‑3 (MQ=255)                     attcaCGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTAGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGttt                                                                                                                                                                                                                <  1:231981/251‑1 (MQ=255)                     agtcaCGCCTGCTATTCCCGTCAGCCTGAGCTGGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGCCCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTAGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGttt                                                                                                                                                                                                                <  2:57022/249‑1 (MQ=255)                      ttcaCGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGGCAAGCCGGAAACCGCCCAGCGCGTTGCCGACTAGTTGGGGAAGAACTTCCTGCAAAATCAGGAAGGtttt                                                                                                                                                                                                               >  2:76878/1‑251 (MQ=255)                         aCGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTAGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGtt                                                                                                                                                                                                            <  1:61887/251‑1 (MQ=255)                                          cAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAATCCGCCCAGCGCGTTGCCGAGTAGGTGGGTAAGAACTACCTGCAAAAGCAGGAAGGTTTTGTTTATATTTGCCGGCTGGa                                                                                                                                                                                           <  2:193427/251‑1 (MQ=255)                                                           gAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTCCTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGACATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTAGTTGGGTAAGCACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACggcgggccgccggg                                                                                                                                                                          >  1:168008/1‑244 (MQ=255)                                                           gAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTAGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAg                                                                                                                                                                          <  2:88185/251‑1 (MQ=255)                                                                    gaAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTAGGTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTGGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAGTACTGGaa                                                                                                                                                                  <  2:165008/250‑1 (MQ=255)                                                                              atcgcggcaCCCTACCGTGAACGGTTACTGCCAGGCTACCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGTCTCTCCGGCTCCGGCGCGTCCTTGTTCGCTCTGTGTGACAAGCCGGACACCGCCCAGCGCGTTGCCGACTAGTTGGTGAAGAACTGCCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCTCGAGTGCTGGAAAACTAAATGaa                                                                                                                                                       <  2:81575/243‑1 (MQ=255)                                                                                   tGAACCCTACCGTGAACGGTTACTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTAGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGGGGACGAggaccgggaaaataaacggaaatcc                                                                                                                                                  >  2:163067/1‑227 (MQ=255)                                                                                    gAACCGTACCGTGAACGGTTACGGCCAGGCTTCCGGCAGGCGGTGAAGGCTGTGGCGGAAAGCGGCGCGGGGGCGAGCGGTAGATACGGCTCCGTCCCGACCTTGTTCGCTCGGTGTGACAAGCCGGAAACCGCCTAGCGCGTTGCCGACTAGTGGGGTAAGAACTACCTGCAAAAGCAGGATGGTTTTGTTCATAGTTGCTGTCTGGATACGGCGGGCGCACGAGTACTGGaaaaagagaggaaactcta                                                                                                                                                 <  1:15663/251‑16 (MQ=255)                                                                                        ccTACCGTGAACGGTTACTGCCCGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTAGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAGTACTGGAAAACTAAATGAAACTCTACAAt                                                                                                                                             <  2:103589/251‑1 (MQ=255)                                                                                             cGTGAACGGTTACTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTAGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCTGGCGCACGAGTACTGGAAAACTAAATGAAACGCTACAATCTGaa                                                                                                                                        <  2:150549/251‑1 (MQ=255)                                                                                              gTGAACGGTTACTGCCAGGGTTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTAGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAGTACTGGAAAACTAAATGAAACTCTACAATCTgtaa                                                                                                                                       <  2:176780/251‑4 (MQ=255)                                                                                                                                   ggcgGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGGTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTAGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAGTACTGGAAAACTAAATGAAACTCTACAATCTGAAAGATCACAACGAGCAGGTCAGCTTTGCGCA‑AGCCGTaa                                                                                                 <  1:40288/251‑1 (MQ=255)                                                                                                                                                     cgcgGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTAGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAGTACTGGAAAACTAAATGAAACTCTACAATCTGAAAGATCACAACGAGCAGGTCAGCTTTGCGCA‑AGCCGTAACCCAGGGGTTGGGCaaaa                                                                               <  2:255948/251‑1 (MQ=255)                                                                                                                                                            gcgagcgGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTAGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACCAGGACTGGGAAACTAAAAGAAAAACTACAATCTTAAAGATCACAACCAACCGGTCAGCTTTGCGCACACCCTAAACCCAGGGGTTGGCCaaaataaggg                                                                         >  1:147822/1‑245 (MQ=255)                                                                                                                                                                             ccccggcGCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTAGTTGGGTAAGAACTTCCTGCAAAATCAGGAAGGTTTTGTGCATATTTGCCGGCTGGATACGGCGGGCGCACGAGTACTGGAAAACTAAATGAAACTCTACAATCTGAAAGATCACAACGAGCAGGTCAGCTTTGCGCA‑AGCCGTAACCCAGGGGTTTGGCAAAAATCA‑GGGGCTGTTTTTGCCGCACg                                                      <  2:168008/249‑1 (MQ=255)                                                                                                                                                                                        ccTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTAGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGGGCGCGAGGAATGGAAAAAAAAAAAAAAAACTAAAAACTGAAAAATCACAAACAGCAGGGGAGGTTTTTGCC‑AGCCCTAACCCCGGGGGTGGGCAAAAATCAAGGGGGGGttttttttccccacccgccggg                                             >  2:223651/1‑234 (MQ=255)                                                                                                                                                                                                                  gAAACCGCCCAGCGCGTTGCCGACTAGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAGTACTGGAAAACTAAATGAAACTCTACAATCTGAAAGATCACAACGAGCAGGTCAGCTTTGCGCA‑AGCCGTAACCCAGGTGTTGGGCAAAAATCA‑GGGGCTGTTTTTTCCGCACGACCTGCCGGAATTCAGCCTGACTGAAATTGATGAGAt                 <  2:12313/251‑1 (MQ=255)                                                                                                                                                                                                                  gAAACCGCCCAGCGCGTTGCCGACTAGGTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAGTACTGGAAAACTAAATGAAACTCTACAATCTGAAAGATCACAACGAGCAGGTCAGCTTTGCGCA‑AGCCGTAACCCAGGGGTTGGGc                                                                                   <  1:241302/186‑1 (MQ=255)                                                                                                                                                                                                                                 gTTGCCGACTAGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAGTACTGGAAAACTAAATGAAACTCTACAATCTGAAAGATCACAACGAGCAGGTCAGCTTTGCGCA‑AGCCGTAACCCAGGGGTTGGGCAAAAATCA‑GGGGCTGTTTTTTCCGCACGACCTGGCGGAATTCAGCCTGACTGAAATTGATGAGATGCTGAAGCTGGAttt  <  1:119587/251‑1 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                   GGCGACATCTGGCAGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAGTACTGGAAAACTAAATGAAACTCTACAATCTGAAAGATCACAACGAGCAGGTCAGCTTTGCGCA‑AGCCGTAACCCAGGGGTTGGGCAAAAATCA‑GGGGCTGTTTTTTCCGCACGACCTGCCGGAATTCAGCCTGACTGAAATTGATGAGATGCTGAAGCTGGATTT  >  NC_000913/3411‑3885

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑