Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,404,402	G→A	S71L (TCG→TTG)	nuoB ←	NADH:quinone oxidoreductase subunit B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,404,402	0	G	A	100.0%	92.8 / NA	26	S71L (TCG→TTG)	nuoB	NADH:quinone oxidoreductase subunit B
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/12);  total (14/12)

TTATCGACGCCCTGCACAACGGAATAAATATCGTACATACCACCAGAGTTGGCACAGGCACCCATTGAGATAACCCATTTTGGTTCCAGCATCTGGTCATACAGACGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGTTTTTGTTAACTTCTTGCTCCAGAGGGTCGGTTACGATCTCCTGCTTTTGCAGGGGGTAACGGTCGTTCTCACCGTTGGGATCTATGCGGGTGAGCGTATAATCCATCTTAATGCCTCGCGGTTAGCGTTGACGATTAGCG  >  NC_000913/2404174‑2404647                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      ttATCGACGCCCTGCACAACGGAATAAATATCGTACATACCACCAGAGTTGGCACAGGCACCCATTGAGATAACCCATTTTGGTTCCAGCATCTGGTCATACAGACGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACAAAGTCACCATCTCAACGTAAc                                                                                                                                                                                                                                  <  2:323999/250‑1 (MQ=255)   aTCGACGCTCTGCACAACGGAATAAATATCGTACATACCACCAGAGTTGGCACAGGCACCCATTGAGATAACCCATTTTGGTTCCAGCATCTGGTCATACAGACGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACAAAGTCACCATCTCAACGTAAcag                                                                                                                                                                                                                                <  1:241368/250‑1 (MQ=255)      gACGCCCTGCACAACGGAATAAATATCGTACATACCACCAGAGTTGGCACAGGCACCCATTGAGATAACCCATTTTGGTTCCAGCATCTGGTCATACAGACGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGAACCGCGGTAAACAAAGTCACCATCTCAACGTAACAGCAgg                                                                                                                                                                                                                            <  2:77696/251‑1 (MQ=255)               cacaACGGAATAAATATCGTACATACCACCAGAGTTGGCACAGGCACCCATTGAGATAACCCATTTTGGTTCCAGCATCTGGTCATACAGACGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACAAAGTCACCATCTCAACGTAACAGCAGGAAAGACCg                                                                                                                                                                                                                    >  1:77139/1‑250 (MQ=255)                             aatCGTACATACCACCAGAGTTGGCACAGGCACCCATTGAGATAACCCATTTTGGTTCCAGCATCTGGTCATACAGACGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACAAAGGCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCaa                                                                                                                                                                                                     <  1:308518/250‑1 (MQ=255)                                          accaGAGTTGGCACAGGCACCCATTGAGATAACCCATTTTGGTTCCAGCATCTGGTCATACAGACGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACAAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTAc                                                                                                                                                                                        >  2:102177/1‑251 (MQ=255)                                                   ggCACAGGCACCCATTGAGATAACCCATTTTGGTTCCAGCATCTGGTCATACAGACGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACAAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGt                                                                                                                                                                               >  1:245885/1‑251 (MQ=255)                                                               cATTGAGTTAACCCATTTTGGATCCAGCATCTGGTCATACAGACGCTGAATAACCGGTGCCATTTTGGTAACGCAAGTTCCTGCAACCACAATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACAAAGTCACCATCTCAACGTAACAGCAGGAAAGACAGAAGTTATACGGCCAAATTGAGCTTTTACGACCCCCGTTAACCATATCAt                                                                                                                                                                   >  2:283285/1‑251 (MQ=255)                                                                            cATTTTGGTTCAAGCATCTGGTCATACAGACGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACAAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCAt                                                                                                                                                      >  2:292260/1‑251 (MQ=255)                                                                                  ggTTCCAGCATCTGGTCATACAGACGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCCCCATCAGGGCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACAAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAaca                                                                                                                                                 <  2:84185/250‑1 (MQ=255)                                                                                    ttCCAGCATCTGGTCATACAGACGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACAAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGt                                                                                                                                              >  2:79637/1‑251 (MQ=255)                                                                                                ggcATACAGACGCTGAATAACCTGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACAAAGTCACCATCTCAACGTAACAGCTGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGGTCGCCCATAAACACGTTTTTGTTAActt                                                                                                                                  <  2:314260/249‑1 (MQ=255)                                                                                                  cctACAGACGCTGAATAACCGGGGCCATTTTGGGAAAGCAGGTTCCTGCAACCACCATCAGGTCATGCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCAGTAAACAAAGTCACCATCTCAACTTAACAGCAGGAAAGACCGAAGTTATACTGCCAAATTGAGTTTTTACGTCCCCAGTTACCCATGTCATTGAGCTTGCCCATAAACACGTTTTTGTTAActtc                                                                                                                                 <  2:321664/248‑1 (MQ=255)                                                                                                   aTACAGACGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACAAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGTTTTTGTTAActtctt                                                                                                                               <  1:6120/251‑1 (MQ=255)                                                                                                   aTACAGACGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACAAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGTTTTTGTTAActtctt                                                                                                                               >  2:7408/1‑251 (MQ=255)                                                                                                          cGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACAAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGTTTTTGTTAACTTCTTGCTcc                                                                                                                          >  1:75674/1‑249 (MQ=255)                                                                                                              gAATGACCGGTGCCCTTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCCGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACAAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATTTCATTGAGCTTGCCCATAAACACGTTTGTGTTAACTTCGTGCTGCagag                                                                                                                      <  2:307913/249‑1 (MQ=255)                                                                                                                                                       accaTCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACAAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGTTTTTGTTAACTTCTTGCTCCAGAGGGTCGGTTACGATCTCCTGCTTTTGCAGGGGGTAACGGTCGtt                                                                           >  1:197593/1‑251 (MQ=255)                                                                                                                                                            cAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACAAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGTTTTTGTTAACTTCTTGCTCCAGAGGGTCGGTTACGATCTCCTGCTTTTGCAGGGGGTAACGGTCGTTCTCAc                                                                      <  2:245885/251‑1 (MQ=255)                                                                                                                                                                                     cGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACAAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGTTTTTGTTAACTTCTTGCTCCAGAGGGTCGGTTACGATCTCCTGCTTTTGCAGGGGGTAACGGTCGTTCTCACCGTTGGGATCTATGCGGGTGAGCGt                                             >  2:275584/1‑251 (MQ=255)                                                                                                                                                                                                 gcgcCAAAACGCGCCACGTCATGCACCGCGGTAAACAAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGTTTTTGTTAACTTCTTGCTCCAGAGGGTCGGTTACGATCTCCTGCTTTTGCAGGGGGTAACGGTCGTTCTCACCGTTGGGATCTATGCGGGTGAGCGTATAATCCATc                                   <  1:238752/249‑1 (MQ=255)                                                                                                                                                                                                             gcCACGTCATGCACCGCGGTAAACAAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGTTTTTGTTAACTTCTTGCTCCAGAGGGTCGGTTACGATCTCCTGCTTTTGCAGGGGGTAACGGTCGTTCTCACCGTTGGGATCTATGCGGGTGAGCGTATAATCCATCTTAATGCCTCGCgg                     >  2:174784/1‑251 (MQ=255)                                                                                                                                                                                                                 cGTCATGCACCGCGGTAAACAAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGTTTTTGTTAACTTCTTGCTCCAGAGGGTCGGTTACGATCTCCTGCTTTTGCAGGGGGTAACGGTCGTTCTCACCGTTGGGATCTATGCGGGTGAGCGTATAATCCATCTTAATGCCTCGCGGtt                   >  1:283403/1‑249 (MQ=255)                                                                                                                                                                                                                  gTCATGCACCGCGGTAAACAAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGTTTTTGTTAACTTCTTGCTCCAGAGGGTCGGTTACGATCTCCTGCTTTTGCAGGGGGTAACGGTCGTTCTCACCGTTGGGATCTATGCGGGTGAGCGTATAATCCATCTTAATGCCTCGCGGTTAGc                >  1:173668/1‑251 (MQ=255)                                                                                                                                                                                                                            gcgGTAAACAAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACTCGTTTTTGTTAGCTTCTTGCTCCAGATGGTCGGTTACGATCTCCTGCTTTTTCAGGGGGTAACGGTCGTTCTCACCGTTGGGATCTATGCTGGTGAGCGTATAAGCCTTCTTAATGCCTCGCGGTTAGCGTTGACGAtt      <  1:282440/251‑1 (MQ=255)                                                                                                                                                                                                                                tAAACAAAGTCACCATATCGACGTAAAAGTAGGAAAGACCGAAGATATAAGGACAAATTGAGATTTTACGACCACAGTTAACCATGTCATTGCGCTTGCCCATAAACACGTTTTTGTTAACTTCTTGCTCCAGAGGGTCGGTTACGATATCCTGCTTTTGCAGGGGGTCACGGGTGGTCTCACCGTTGGGATCTATGAGGGCGAGCGTATAATCCATATTAATGCCTCGCGGATAGCGTTGACGATTAGCg  >  1:101872/1‑251 (MQ=255)                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      TTATCGACGCCCTGCACAACGGAATAAATATCGTACATACCACCAGAGTTGGCACAGGCACCCATTGAGATAACCCATTTTGGTTCCAGCATCTGGTCATACAGACGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGTTTTTGTTAACTTCTTGCTCCAGAGGGTCGGTTACGATCTCCTGCTTTTGCAGGGGGTAACGGTCGTTCTCACCGTTGGGATCTATGCGGGTGAGCGTATAATCCATCTTAATGCCTCGCGGTTAGCGTTGACGATTAGCG  >  NC_000913/2404174‑2404647

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑