Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,524,913	C→T	F681F (TTC→TTT)	mrcA →	peptidoglycan glycosyltransferase/peptidoglycan DD‑transpeptidase MrcA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,524,913	0	C	T	100.0%	108.1 / NA	33	F681F (TTC→TTT)	mrcA	peptidoglycan glycosyltransferase/peptidoglycan DD‑transpeptidase MrcA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/19);  total (14/19)

GGCGTGATTTTCGAAGCGAAACCGAAAGTAGCCTGCCCGGAATGCGATATTCCGGTGATTTACGGTGATACGCAGAAATCGAACGTGCTGGAAAATAACGATGTTGAAGATGTCGCTATCTCCCGCGAGCAGCAGAATGTTTCTGTACCAATGCCGCAGCTGGAGCAGGCAAATCAGGCGTTAGTGGCGAAGACTGGCGCGCAGGAGTACGCACCGCACGTCATCAACACTCCGCTGGCATTCCTGATTAAGAGTGCTTTGAACACCAATATCTTTGGTGAGCCAGGCTGGCAGGGTACTGGCTGGCGTGCAGGTCGTGATTTGCAGCGTCGCGATATCGGCGGGAAAACCGGGACCACTAACAGTTCGAAAGATGCGTGGTTCTCGGGTTACGGTCCGGGCGTTGTGACCTCGGTCTGGATTGGCTTTGATGATCACCGTCGTAATCTCGGTCATACAACGGCTTCCGGAGCGATTAAAGAT  >  NC_000913/3524671‑3525153                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                 ggcgTCATTTTCGAAGCGAAACCGCAAGTCGCCCGCCCGGAATGCGATATTCCGGTGATATACGGTGATACGCAGAAATCGACAGTGATGGCACATAACGATGTTGAAGATGTCGCTATCTCCCGCGAGCAGCAGAATGTTTCTGTACAAATGCCGCAGCTGGAACAGGCAAATCAGGCGTTAGTGGCGAAGACTGCCGCGCCGGAGGACGCACCACACGTCACCAACACTCCGCTGGCATTTCTGAttc                                                                                                                                                                                                                                           >  2:24543/1‑249 (MQ=255)   cgTGATTTTCGAAGCGAAACCGAAAGTAGCCTGCCCGGAATGCGATATTCCGGTGATTTACGGTGATACGCAGAAATCGAACGTGCTGGAAAATAACGATGTTGAAGATGTCGCTATCTCCCGCGAGCAGCAGAATGTTTCTGTACCAATGCCGCAGCTGGAGCAGGCAAATCAGGCGTTAGTGGCGAAGACTGGCGCGCAGGAGTACGCACCGCACGTCATCAACACTCCGCTGGCATTTCTGATTAaga                                                                                                                                                                                                                                        <  1:66862/251‑1 (MQ=255)      gagtttCGAAGCGAAACCGAAAGTAGCCTGCCCGGAATGCGATATTCCGGTGATTTACGGTGATACGCAGAAATCGAACGTGCTGGAAAATAACGATGTTGAAGATGTCGCTATCTCCCGCGAGCAGCAGAATGTTTCTGTACCAATGCCGCAGCTGGAGCAGGCAAATCAGGCGTTAGTGGCGAAGACTGGCGCGCAGGAGTACGCACCGCACGTCATCAACACTCCGCTGGCATTTCTGATTAAGAGTg                                                                                                                                                                                                                                     <  2:262900/248‑1 (MQ=255)             gAAGCGAAACCGAAAGTAGCCTGCCCGGAATGCGATATTCCGGTGATTTACGGTGATACGCAGAAATCGAACGTGCTGGAAAATAACGATGTTGAAGATGTCGCTATCTCCCGCGAGCAGCAGAATGTTTCTGTACCAATGCCGCAGCTGGAGCAGGCAAATCAGGCTTTAGTGGCGAAGGGTGGCGCGCAGGAGTACGGACCGCACGGCATCAACACTCCGCTGGCAATTGTGATTAAGAGTGCTTTGaa                                                                                                                                                                                                                              <  2:140666/251‑1 (MQ=255)              aaGCGAAACCGAAAGTAGCCTGCCCGGAATGCGATATTCCGGTGATTTACGGTGATACGCAGAAATCGAACGTGCTGGAAAATAACGATGTTGAAGATGTCGCTATCGCCCGCGAGCAGCAGAATGTTTCTGTACCAATGCCGCAGCTGGAGCAGGCAAATCAGGCGTTAGTGGCGAAGACTGGCGCGCAGGAGTACGCACCGCACGTCATCAACACTCCGCTGGCATTTCTGATTAAGAGTGCTTTGAac                                                                                                                                                                                                                             <  2:94024/251‑1 (MQ=255)                   aaaCCGAAAGTAGCCTGCCCGGAATGCGATATTCCGGTGATTTACGGTGATACGCAGAAATCGAACGTGCTGGAAAATAACGATGTTGAAGATGTCGCTATCTCCCGCGAGCAGCAGAATGTTTCTGTACCAATGCCGCAGCTGGAGCAGGCAAATCAGGCGTTAGTGGCGAAGACTGGCGCGCAGGAGTACGCACCGCACGTCATCAACACTCCGCTGGCATTTCTGATTAAGAGTGCTTTGAACACCaa                                                                                                                                                                                                                        <  2:100093/251‑1 (MQ=255)                             gaGCCTGGCCGGAATGCGATACTACGGTGATTTAAGGTGATACGCAGAAATCGAACGTGCGGGAAAAGAACGATGTTGAAGATGTCGCTATCTCCCGCGAGCAGCAGAATGTTTCGGGACCAATGCCGCAGCTGGAGCATGCAAATCAGGCGTGAGTGGCGAAGACTGGCGCGCAGGAGGACGCACCGCGCGTCACCAACTCTCCGCGCGCAATTCGGAGTAAGAGTGCGTTGTACTCCAATATGTTTGGt                                                                                                                                                                                                              <  2:212853/250‑1 (MQ=255)                                   gCCCGGACTGCGATATTCCGGTGATTTACGGTGATACGCAGAAATCGAACGTGCTGGAAAATAACGATGTTGAAGATGTCGCTATCTCCCGCGAGCAGCAGAATGTTTCTGTACCAATGCCGCAGCTGGAGCAGGCAAATCAGGCGTTAGTGGCGAAGACTGGCGCGCAGGAGTACGCACCGCACGTCATCAACACTCCGCTGGCATTTCTGATTAAGAGTGCTTTGAACACCAATATCTTTGGTGAGcc                                                                                                                                                                                                         >  2:135766/1‑250 (MQ=255)                                            gCGATATTCCGGTGATTTACGGTGATACGCAGAAATCGAACGTGCTGGAAAATAACGATGTTGAAGATGTCGCTATCTCCCGCGAGCAGCAGAATGTTTCTGTACCAATGCCGCAGCTGGAGCAGGCAAATCAGGCGTTAGTGGCGAAGACTGGCGCGCAGGAGTACGCACCGCACGTCATCAACACTCCGCTGGCATTTCTGATTAAGAGTGCTTTGAACACCAATATCTTTGGTGAGCCAGGCTGGCAg                                                                                                                                                                                               <  2:275739/251‑1 (MQ=255)                                             cGATATTCCGGTGATTTACGGTGATACGCAGAAATCGAACGTGCTGGAAAATAACGATGTTGAAGATGTCGCTATCTCCCGCGAGCAGCAGAATGTTTCTGTACCAATGCCGCAGCTGGAGCAGGCAAATCAGGCGTTAGTGGCGAAGACTGGCGCGCAGGAGTACGCACCGCACGTCATCAACACTCCGCTGGCATTTCTGATTAAGAGTGCTTTGAACACCAATATCTTTGGTGAGCCAGGCTGGCAgg                                                                                                                                                                                              >  1:52049/1‑251 (MQ=255)                                              gATATTCCGGTGATTTACGGTGATACGCAGAAATCGAACGTGCTGGAAAATAACGATGTTGAAGATGTCGCTATCTCCCGCGAGCAGCAGAATGTTTCTGTACCAATGCCGCAGCTGGAGCAGGCAAATCAGGCGTTAGTGGCGAAGACTGGCGCGCAGGAGTACGCACCGCACGTCATCAACACTCCGCTGGCATTTCTGATTAAGAGTGCTTTGAACACCAATCTCTTTGGTGAGCCAGGCTGGCAggg                                                                                                                                                                                             >  2:64935/1‑251 (MQ=255)                                                            ttACGGTGAGACGCAGAAATCGAACGTGCTGGAAAATAACGATGTTGAAGATGTCGCTATCGCCCGCGAGCAGCAGAATGTTTCTGTACCAATGCCGCAGCTGGAGCAGGCAAATCAGGCGTTAGTGGCGAAGACTGGCGCGCAGGAGTACGCACCGCACGTCATCAGCACTCCGCTGGCATTTCTGATTAAGAGTGCTTTGAACGCCAATATCTTTGGTGAGCCAGGCTGGCAGGGTACTGGCTGGCGt                                                                                                                                                                                <  2:38865/250‑1 (MQ=255)                                                               cggagATACGCAGAAATCGAACGTGCTGGAAAAGAACGATGTTGAAGAGGTCGCTATCCCCCGCGAGCAGCAGAATGTTTCTGTACCAATGCCGCAGCTGGAGCAGGCAAATCAGGCGTTAGTGGCGAAGACTGGCGCGCAGGAGTACGCACCGCACGTCATCAACACTCCGCTGGCATTTCTGATTAAGAGTGCTTTGAACACCAATATCTTTGGTGAGCCAGGCTGGCAGGGTACTGGCTGGCGTGCAg                                                                                                                                                                            <  1:144361/247‑1 (MQ=255)                                                                        gCAGAAATCGCACATGCTGAAAACTAACGATGTTGAAGATGTCGCTATCTCCCGCGAGCAGCAGAATGTTTCTGTACCAATGCCGCAGCTAAAGCAGGCAAATCAGGCGTTAGTGGCGAAGACTGGCGCGCAGGAGTCCGCACCGCACGTCATCAACACTCCGCTGGCATTTATGCTTAAGAGTGCTTTGAACACCAATATCTTTGGTGTGCCAGGCTGGCAGGGTACAGGCTGGCGTGCAGGACGTggtt                                                                                                                                                                   >  2:291864/1‑248 (MQ=255)                                                                                          ggAAAATAACGATGTTGAAGATGTCGCTATCTCCCGCGAGCAGCAGAATGTTTCTGTACCAATGCCGCAGCTGGAGCAGGCAAATCAGGCGTTAGTGGCGAAGACTGGCGCGCAGGAGTACGCACCGCACGTCATCAACACTCCGCTGGCATTTCTGATTAAGAGTGCTTTGAACACCAATATCTTTGGTGAGCCAGGCTGGCAGGGTACTGGCTGGCGTGCAGGTCGTGATTTGCAGCGTCGCGatat                                                                                                                                                   <  2:180875/249‑1 (MQ=255)                                                                                          ggAAAATAACGATGTTGAAGATGTCGCTATCTCCCGCGAGCAGCAGAATGTTTCTGTACCAATGCCGCAACTGGAGCAGGCAAATCAGGCGTTAGTGGCGAAGACTGGCGCGCAGGAGTACGCACCGCACGTCATCAAAACTCCGCTGGCATTTCTGATTACGAGTGCTTTGAACACCCATATCTTTGGTGCGCCAGGCTGGCTGGGTACTGGCTGGCGTGCAGGTCGCGATTTGCAGCGTAGCGATATcg                                                                                                                                                 >  1:322399/1‑251 (MQ=255)                                                                                                tAACGATGTTGAAGATGTCGCTATCTCCCGCGCGCAGCAGAATGTTTCTGTACCAATGCCGCAGCTGGAGCAGGCAAATCAGGCGTTAGTGGCGAAGACTGGCGCGCAGGAGTACGCACCGCACGTCATCAACACTCCGCTGGCATTTCTGATTAAGAGTGCTTTGAACACCAATATCTTTGGTGAGCCAGGCTGGCAGGGTACTGGCTGGCGTGCAGGTCGTGATTTGCAGCGTCGCGATATCGGCgggg                                                                                                                                           >  2:307548/1‑250 (MQ=255)                                                                                                 aaCGATGTTGAAGATGTCGCTATCTCCCGCGAGCAGCAGAATGTTTCTGTACCAATGCCGCAGCTGGAGCAGGCAAATCAGGCGTTAGTGGCGAAGACTGGCGCGCAGGAGTACGCACCGCACGTCATCAACACTCCGCTGGCATTTCTGATTAAGAGTGCTTTGAACACCAATATCTTTGGTGAGCCAGGCTGGCAGGGTACTGGCTGGCGTGCAGGTCGTGATTTGCAGCGTCGCGATATCGGCgggg                                                                                                                                           >  1:132982/1‑249 (MQ=255)                                                                                                    gATGTTGAAGATGTCGCTATCTCCCGCGAGCAGCAGAATGTTTCTGTACCAATGCCGCAGCTGGAGCAGGCAAATCAGGCGTTAGTGGCGAAGACTGGCGCGCAGGAGTACGCACCGCACGTCATCAACACTCCGCTGGCATTTCTGATTAAGAGTGCTTTGAACACCAATATCTTTGGTGAGCCAGGCTGGCAGGGTACTGGCTGGCGTGCAGGTCGTGATTTGCAGCGTCGCGATATCGGCGGGaaaa                                                                                                                                        >  1:242067/1‑250 (MQ=255)                                                                                                       gTTGAAGATGTCGCTATCTCCCGCGAGCAGCAGAATGTTTCTGTACCAATGCCGCAGCTGGAGCAGGCAAATCAGGCGTTAGTGGCGAAGACTGGCGCGCAGGAGTACGCACCGCACGTCATCAACACTCCGCTGGCATTTCTGATTAAGAGTGCTTTGAACACCAATATCTTTGGTGAGCCAGGCTGGCAGGGTACTGGCTGGCGTGCAGGTCGTGATTTGCAGCGTCGCTATATCGGCGGGAGAAcccg                                                                                                                                    >  1:138555/1‑249 (MQ=255)                                                                                                                      aTCTCCCGCGAGCAGCAGAATGTTTCTGTACCAATGCCGCAGCTGACGCAGGCAAATCAGGCGTTAGTGGCGAAGACTGGCGCGCAGGAGTCCGCACCGCACGTCATCAACACCCCGCTGGCATTTCTGATTAAGAGTGCTTTGAACACCAATATCTTTGGTGAGCCAGGCTGGCAGGGTACTGGCTGGCGTGCAGGTCGTGATTTGCAGCGTCGCGATATCGGCGGGTCCACCGGGAccccaaacacatc                                                                                                                     >  1:136718/1‑240 (MQ=255)                                                                                                                       tctcCCGCGAGCAGCAGAATGTTTATGTACCAATGCCGCAGCTGGAGCAGGCAAATCAGGCGTTAGTGGCGAAGACTGGCGCGCAGGAGTACGCACCGCACGTCATCAACACTCCGCTGGCATTTCTGATTAAGAGTGCTTTGAACACCAATATCTTTGGTGAGCCAGGCTGGCAGGGTACTGGCTGGCGTGCAGGTCGTGATTTGCAGCGTCGCGATATCGGCGGGGAAACCCGGACGACTaccaatttg                                                                                                                    >  2:149265/1‑243 (MQ=255)                                                                                                                        ctcCCGCGAGCAGGAGAATGTTTCTGTACCAATGCCGCAGCTGGAGCAGGCAAATCAGGCGTTAGTGGCGAAGACTGGCGCGCAGGAGTACGCACCGCACGTCATCAACACTCCGCTGGCATTTCTGATTAAGAGTGCTTTGAACACCAATATCTTTGGTGAGCCAGGCTGGCAGGGTACTGGCTGGCGTGCAGGTCGTGATTTGCAGCGGCGCGATATCGGCgggggaaacagcgggcctcacatctgtc                                                                                                                   >  2:159163/1‑226 (MQ=255)                                                                                                                                       gAATGTTTCTGTACCAATGCCGCAGCTGGAGCAGGCAAATCAGGCGTTAGTGGCGAAGACTGGCGCGCAGGAGTACGCACCGCACGTCATCAACACTCCGCTGGCATTTCTGATTAAGAGTGCTTTGAACACCAATATCTTTGGTGAGCCAGGCTGGCAGGGTACTGGCTGGCGTGCAGGTCGTGATTTGCAGCGTCGCGATATCGGCGGGAAAACCGGGACCACTAACAGTTCGAAAGATGCGTGGTtct                                                                                                    <  1:64935/251‑1 (MQ=255)                                                                                                                                         aTGTTTCTGTACCAATGCCGCAGCTGGAGCAGGCAAATCAGGCGTTAGTGGCGAAGACTGGCGCGCAGGAGTACGCACCGCACGTCATCAACACTCCGCTGGCATTTCTGATTAAGAGTGCTTTGAACACCAATATCTTTGGTGAGCCAGGCTGGCAGGGTACTGGCTGGCGTGCAGGTCGTGATTTGCAGCGTCGCGATATCGGCGGGAAAACCGGGACCACTAACAGTTCGAAAGATGCGTGGTTCTCg                                                                                                  <  2:261112/251‑1 (MQ=255)                                                                                                                                                                    aGCAGGCAAATCAGGCGTTAGTGGCGAAGACTGGCGCGCAGGAGTACGCACCGCACGTCATCAACACTCCGCTGGCATTTCTGATTAAGAGTGCTTTGAACACCAATATCTTTGGTGAGCCAGGCTGGCAGGGTACTGGCTGGCGTGCAGGTCGTGATTTGCAGCGTCGCGATATCGGCGGGAAAACCGGGACCACTAACAGTTCGAAAGATGCGTGGTTCTCGGGTTACGGTCCGGGCGTTGTGACCTCg                                                                       <  2:132982/251‑1 (MQ=255)                                                                                                                                                                                   cGTTAGTGGCTAAGACTGGCGCGCAGGAGTACGCACCGCACGTCATCAACACTCCGCTGGCATTTCTGATTAAGAGTGCTTTGAACTCCAATATCTTTGGTGAGCCAGGCTGGCAGGGTACTGGCTGGCGTGCAGGTCGTGATTTGCAGCGTCGCGATATCGGCGGGAAAACCGGGACCACTAACAGTTCGAAAGATGCGTGGTTCTCGGGTTACGGTCCGGGCGTTGTGACCCCGGTCTGGATTGGCttt                                                        <  1:117169/251‑1 (MQ=255)                                                                                                                                                                                       aGTGGCGAAGACTGGCGCGCAGGAGTACGCACCGCACGTCATCAACACTCCGCTGGCATTTCTGATTAAGAGTGCTTTGAACACCAATATCTTTGGTGAGCCAGGCTGGCAGGGTACTGGCTGGCGTGCAGGTCGTGATTTGCAGCGTCGCGATATCGGCGGGAAAACCGGGACCACTAACAGTTCGAAAGATGCGTGGTTCTCGGGTTACGGTCCGGGCGTTGTGACCTCGGTCTGGATTGGCTTtgat                                                     <  2:85547/250‑1 (MQ=255)                                                                                                                                                                                             gAAGACTGGCGCGCAGGAGTACGCACCGCACGTCATCAACACTCCGCTGGCATTTCTGATTAAGAGTGCTTTGAACACCAATATCTTTGGTGAGCCAGGCTGGCAGGGTACTGGCTGGCGTGCAGGTCGTGATTTGCAGCGTCGCGATATCGGCGGGAAAACCGGGACCACTAACAGTTCGAAAGATGCGTGGTTCTCGGGTTACGGTCCGGGCGTTGTGACCTCGGTCTGGATTGGCTTTGATGATCAcc                                              <  2:52049/251‑1 (MQ=255)                                                                                                                                                                                              aaGACTGGCGCGCAGGAGTACGCACCGCACGTCATCAACACTCCGCTGGCATTTCTGATTAAGAGTGCTTTGAACACCAATATCTTTGGTGAGCCAGGCTGGCAGGGTACTGGCTGGCGTGCAGGTCGTGATTTGCAGCGTCGCGATATCGGCGGGAAAACCGGGACCACTAACAGTTCGAAAGATGCGTGGTTCTCGGGTTACGGTCCGGGCGTTGTGACCTCGGTCTGGATTGGCTTTGATGATCACcg                                             <  1:149265/251‑1 (MQ=255)                                                                                                                                                                                                    ggCGCGCAGGAGTACGCACCGCACGTCATCAACACTCCGCTGGCATTTCTGATTAAGAGTGCTTTGAACACCAATATCTTTGGTGAGCCAGGCTGGCAGGGTACTGGCTGGCGTGCAGGTCGTGATTTGCAGCGTCGCGATATCGGCGGGGAAACCGGGGGCACTAACAGGTCGGAAAATGGGGGGTTGTCGGGGTACGGTCCGGGGGGTTTGACCCCCGACTGGATTGGCTTtgatgttccacggcgta                                        >  1:234234/1‑238 (MQ=255)                                                                                                                                                                                                                 aCGCACCGCACGTCATCAACACTCCGCTGGCATTTCTGATTAAGAGTGCTTTGAACACCAATATCTTTGGTGAGCCAGGCTGGCAGGGTACTGGCTGGCGTGCAGGTCGTGATTTGCAGCGTCGCGATATCGGCGGGAAAACCGGGACCACTAACAGTTCGAAAGATGCGTGGTTCTCGGGTTACGGTCCGGGCGTTGTGACCTCGGTCTGGATTGGCTTTGATGATCTCCGTCGTAATCTCGGTCATACa                          <  1:257456/251‑1 (MQ=255)                                                                                                                                                                                                                                         cGCTGGCATTTCTGATTAAGAGTGCTTTGAACTCCAATATCTTTGGTGAGCCAGGCTGGCAGGGTACTGGCTGGCGTGCAGGTCGTGATTTGCAGCGTCGCGATAGCGGCGGGAAAACCGGGACCACTAACAGTTCGAAAGATGCGTGGTTCTCGGGTTACGGTCCGGGCGTTGTGACCTCGGTCTGGATTGGCTTTGATGATCACCGTCGTAATCTCGGTCATACAACGGCTTCCGGAGCGATTAAagat  <  2:230556/251‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                 GGCGTGATTTTCGAAGCGAAACCGAAAGTAGCCTGCCCGGAATGCGATATTCCGGTGATTTACGGTGATACGCAGAAATCGAACGTGCTGGAAAATAACGATGTTGAAGATGTCGCTATCTCCCGCGAGCAGCAGAATGTTTCTGTACCAATGCCGCAGCTGGAGCAGGCAAATCAGGCGTTAGTGGCGAAGACTGGCGCGCAGGAGTACGCACCGCACGTCATCAACACTCCGCTGGCATTCCTGATTAAGAGTGCTTTGAACACCAATATCTTTGGTGAGCCAGGCTGGCAGGGTACTGGCTGGCGTGCAGGTCGTGATTTGCAGCGTCGCGATATCGGCGGGAAAACCGGGACCACTAACAGTTCGAAAGATGCGTGGTTCTCGGGTTACGGTCCGGGCGTTGTGACCTCGGTCTGGATTGGCTTTGATGATCACCGTCGTAATCTCGGTCATACAACGGCTTCCGGAGCGATTAAAGAT  >  NC_000913/3524671‑3525153

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑