Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	884,892	C→T	S407F (TCT→TTT)	mdfA →	multidrug efflux pump MdfA/Na(+):H(+) antiporter/K(+):H(+) antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	884,892	0	C	T	100.0%	59.8 / NA	18	S407F (TCT→TTT)	mdfA	multidrug efflux pump MdfA/Na(+):H(+) antiporter/K(+):H(+) antiporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/6);  total (12/6)

ATTGGTCTGGCGAATGCGGGACTGGTGCGATTAACCCTGTTTGCCAGCGATATGAGTAAAGGTACGGTTTCTGCCGCGATGGGAATGCTGCAAATGCTGATCTTTACCGTTGGTATTGAAATCAGCAAACATGCCTGGCTGAACGGGGGCAACGGACTGTTTAATCTCTTCAACCTTGTCAACGGAATTTTGTGGCTGTCGCTGATGGTTATCTTTTTAAAAGATAAACAGATGGGAAATTCTCACGAAGGGTAAAAAAATGCCTGACTGCTTTGTGCGATCAGGCATTCTCGAATTAATGGTGATGGTCGTCAATCTGGTGTTCGATAACCATCCCTTCACCTACGCTGGCAAGATGGCGAACATAAGGATGCGGGCGGTAAGCCGGAGCTGGCGCAGGAGCCACATAGACGGTTTGCGGCACAGTCGCGACGCTGACCGCTTGTGGAACGCTGACTGAATC  >  NC_000913/884651‑885113                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                              aTTGGTCTGGCGAATGCGGGACTGGTGCGATTAACCCTGTTTGCCAGCGATATGAGTAAAGGTACGGTTTCTGCCGCGATGGGAATGCTGCAAATGCTGATCTTTACCGTTGGTATTGAAATCAGCAAACATGCCTGGCTGAACGGGGGCAACGGACTGTTTAATCTCTTCAACCTTGTCAACGGAATTTTGTGGCTGTCGCTGATGGTTATCTTTTTAAAAGATAAACAGATGGGAAATTTTCACGCAgg                                                                                                                                                                                                                      >  2:50465/1‑251 (MQ=255)  ttGGTCTGGCGAATGCGGGACTGGTGCGATTAACCCTGTTTGCCAGCGATATGAGTAAAGGTACGGATTCTGCCGCGATGGGAATGCTGCAAATGCTGATCTTTACCGATGGTATTGAAATCAGCAAACATGCCTGGCTGAACGGGGGCAACGGACTGTTTAATCTCTTCAACCTTGTCAACGGAATTTTGTGGCTGTCGCTGATGGTTATCTTTTTAAAAGATAAACAGATGGGAAATTTTCACGAAggg                                                                                                                                                                                                                     >  1:191715/1‑251 (MQ=255)    ggTCTGGCGAGTGCGGGACTGGTGCGCTTAACCCTGTTTGCCAGCGATATGAGTAAAGGTACGGTTTCTGCCGCGATGGGAATGCTGCAAATGCTGATCTTTACCGTTGGTATTGAAATCAGCAAACATGCCTGGCTGAACGGGGGCAACGGACTGTTTAATCTCTTCAACCTTGTCAACGGACTTTTGTGGCTGTCGCTGATGGTTATCTTTTTAAAAGATAAACAGATGGGAAATTTTCACGAAGGGt                                                                                                                                                                                                                    >  2:215399/1‑250 (MQ=255)                        ggTGCGATTAACCCTGTTTGCCAGCGATATGAGTAAAGGTACGGTTTCTGCCGCGATGGGAATGCTGCAAATGCTGATCTTTACCGTTGGTATTGAAATCAGCAAACATGCCTGGCTGAACGGGGGCAACGGACTGTTTAATCTCTTCAACCTTGTCAACGGAATTTTGTGGCTGTCGCTGATGGTTATCTTTTTAAAAGATAAACAGATGGGAAATTTTCACGAAGGGTAAAAAAATGCCTGACTGCtt                                                                                                                                                                                                <  1:290655/250‑1 (MQ=255)                                                     tGAGTAAAGGTACGGTTTCTGCCGCGATGGGAATGCTGCAAATGCTGATCTTTACCGTTGGTATTGAAATCAGCAAACATGCCTGGCTGAACGGGGGCAACGGACTGTTTAATCTCTTCAACCTTGTCAACGGAATTTTGTGGCTGTCGCTGCTGGTTATCTTTTTAAAAGATAAACAGATGGGAAATTTTCACGAAGGGTAAAAAAATGCATGACTGCTTTGTGCGATCAGGCATTCTCGAATTAATGGt                                                                                                                                                                  >  1:159445/1‑251 (MQ=255)                                                                aCGGTTTCTGCCGCGATGGGAATGCTGCAAATGCTGATCTTTACCGTTGGTATTGAAATCAGCAAACATGCCTGGCTGAACGGGGGCAACGGACTGTTTAATCTCTTCAACCTTGTCAACGGAATTTTGTGGCTGTCGCTGATGGTTATCTTTTTAAAAGATAAACAGATGGGAAATTTTCACGAAGGGTAAAAAAATGCCTGACTGCTTTGTGCGATCAGGCATTCTCGAATTAATGGTGATGgtcgtc                                                                                                                                                        >  2:202589/1‑250 (MQ=255)                                                                                                                          tCAGCAAACATGCCTGGCTGAACGGGGGCAACGGACTGTTTAATCTCTTCAACCTTGTCAACGGAATTTTGTGGCTGTCGCTGATGGTTATCTTTTTAAAAGATAAACAGATGGGAAATTTTCACGAAGGGTAAAAAAATGCCTGACTGCTTTGTGCGATCAGGCATTCTCGAATTAATGGTGATGGTCGTCAATCTGGTGTTCGATAACCATCCCTTCACCTACGCTGGCAAGATGGCGAACATAAGGAt                                                                                             >  1:181896/1‑251 (MQ=255)                                                                                                                                             gAACGGGGGCAACGGACTGTTTAATCTCTTCAACCTTGTCAACGGAATTTTGTGGCTGTCGCTGATGGTTATCTTTTTAAAAGATAAACAGATGGGAAATTTTCACGAAGGGTAAAAAAATGCCTGACTGCTTTGTGCGATCAGGCATTCTCGAATTAATGGTGATGGTCGTCAATCTGGTGTTCGATAACCATCCCTTCACCTACGCTGGCAAGATGGCGAACATAAGGATGCGGGCGGTAAGCCGGa                                                                            <  2:243584/249‑1 (MQ=255)                                                                                                                                                              tGTTTAATCTCTTCAACCTTGTCAACGGAATTTTGTGGCTGTCGCTGATGGTTATCTTTTTAAAAGATAAACAGCTGGGAAATTTTCACGAAGGGTAAAAAAATGCCTGACTGCTTTGTGCGATCAGGCATTCTCGAATTAATGGTGATGGTCGTCAATCTGGTGTTCGATAACCATCCCTTCACCTACGCTGGCAAGATGGCGAACATAAGGATGCGGGCGGTAAGCCGGAGCTGGCGCAGGAGCCACAt                                                         >  1:51037/1‑251 (MQ=255)                                                                                                                                                                                  gTCAACGGAATTTTGTGGCTGTCGCTGATGGTTATCTTTTTAAAAGATGAACAGATGGGACATTTTCACGAAGGGTAAAAAAATGCCTGACTGCTTTGTGCGATCAGGCATTCTCGAATTAATGGAGATGGTCGTCAATCTGGTGTTCGATAACCATCCCTTCACCTTCGCTGGCAAGATGGCGAACATAAGGATGCGGGCGGTAAGCCGGAGCTGGCGCAGGAGCCACATAGACGGTTTGCGGCACAGt                                      >  2:14063/1‑250 (MQ=255)                                                                                                                                                                                        ggAATTTTGTGGCTGTCGCTGATGGTTATCTTTTTAAAAGATAAACAGCTGGGAAATTTTCACGAAGGGTAAAAAAATGCCTGACTGCTTTGTGCGATCAGGCATTCTCGAATTAATGGTGATGGTCGTCAATCTGGTGTTCGATAACCATCCCTTCACCTACGCTGGCAAGCTGGCGaa                                                                                                      >  1:114911/1‑180 (MQ=255)                                                                                                                                                                                        ggAATTTTGTGGCTGTCGCTGATGGTTATCTTTTTAAAAGATAAACAGATGGGAAATTTTCACGAAGGGTAAAAAAATGCCTGACTGCTTTGTGCGATCAGGCATTCTCGAATTAATGGTGATGGTCGTCAATCTGGTGTTCGATAACCATCCCTTCACCTACGCTGGCAAGATGGCGaa                                                                                                      <  2:114911/180‑1 (MQ=255)                                                                                                                                                                                                              tGGTTCTCTTTTTAAAAGATAAACAGATGGGAAATTTTCACGAAGGGTAAAAAAATGCCTGACTGCTTTGTGCGATCAGGCATTCTCGAATTAATGGTGATGGTCGTCAATCTGGTGTTCGATAACCa                                                                                                                                    >  2:193766/1‑128 (MQ=255)                                                                                                                                                                                                              tGGTTATCTTTTTAAAAGATAAACAGATGGGAAATTTTCACGAAGGGTAAAAAAATGCCTGACTGCTTTGTGCGATCAGGCATTCTCGAATTAATGGTGATGGTCGTCAATCTGGTGTTCGATAACCa                                                                                                                                    <  1:193766/128‑1 (MQ=255)                                                                                                                                                                                                                     cTTTTTAAAAGATAAACAGATGGGAAATTTTCACGAAGGGTAAAAAAATGCCTGACTGCTTTGTGCGATCAGGCATTCTCGAATTAATGGTGATGGTCGTCAATCTGGTGTTCGATAACCATCCCTTCACCTACGCTGGCAAGATGGCGAACATAAGGATGCGGGCGGTAAGCCGGAGCTGGCGCAGGAGCCACATAGACGGTTTGCGGCACAGTCGCGACGCTGACCGCTTGTGGAACGCTGACTGAATc  >  2:55392/1‑251 (MQ=255)                                                                                                                                                                                                                       ttttAAAAGATAAACAGATGGGAAATTTTCACGAAGGGTAAAAAAATGCCTGACTGCTTTGTGCGATCAGGCATTCTCGAATTAAGGGGGATGGTCGTCAATCTGGTGTTTGATAACCATCCCTTCTCCTACGCTGGCAAGATGGCGAACATAAGGATGCGGGCGGTAAGCCGGAGCTGGCGCAGGAGCCACATAGACGGTTTGCGGCACAGTCGCGACGCTGACCGCTTGTGGACCGCTGACTGAATc  <  2:28920/249‑1 (MQ=255)                                                                                                                                                                                                                              aGATAAACAGATGGGAAATTTTCACGAAGGGTAAAAAAATGCCTGACTGCTTTGTGCGATCAGGCATTCTCGAATTAATGGTGATGGTCGTCAATCTGGTGTTCGATAACCATCCCTTCACCTACGCTGGCAAGATGGCGAACATAAGGATGCGGGCGGTAAGCCGGAGCTGGCGCAGGAGc                                                              <  1:94020/182‑1 (MQ=255)                                                                                                                                                                                                                              aGATAAACAGATGGGAAATTTTCACGAAGGGTAAAAAAATGCCTGACTGCTTTGTGCGATCAGGCATTCTCGAATTAATGGTGATGGTCGTCAATCTGGTGTTCGATAACCATCCCTTCACCTACGCTGGCAAGATGGCGAACATAAGGATGCGGGCGGTAAGCCGGAGCTGGCGCAGGAGc                                                              >  2:94020/1‑182 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                              ATTGGTCTGGCGAATGCGGGACTGGTGCGATTAACCCTGTTTGCCAGCGATATGAGTAAAGGTACGGTTTCTGCCGCGATGGGAATGCTGCAAATGCTGATCTTTACCGTTGGTATTGAAATCAGCAAACATGCCTGGCTGAACGGGGGCAACGGACTGTTTAATCTCTTCAACCTTGTCAACGGAATTTTGTGGCTGTCGCTGATGGTTATCTTTTTAAAAGATAAACAGATGGGAAATTCTCACGAAGGGTAAAAAAATGCCTGACTGCTTTGTGCGATCAGGCATTCTCGAATTAATGGTGATGGTCGTCAATCTGGTGTTCGATAACCATCCCTTCACCTACGCTGGCAAGATGGCGAACATAAGGATGCGGGCGGTAAGCCGGAGCTGGCGCAGGAGCCACATAGACGGTTTGCGGCACAGTCGCGACGCTGACCGCTTGTGGAACGCTGACTGAATC  >  NC_000913/884651‑885113

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑