Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 154,182 C→T L415L (CTG→CTA htrE ← putative fimbrial usher protein HtrE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913154,1820CT95.2% 68.5 / NA 21L415L (CTG→CTAhtrEputative fimbrial usher protein HtrE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (15/5);  minor base G (0/1);  total (15/6)
Fisher's exact test for biased strand distribution p-value = 2.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

TTCGGTTCAAGATCTTGTTCGGGATGTTTCACTTCATCAATTAGAGTTAGTGCATCATTAAGACCAAGGTAATTCTGTGTCGAATAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTCAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGTAACATTTGAACAACGGATGAGAAAGGTTGCGAG  >  NC_000913/153946‑154395
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                     
ttCGGTTCAAGATCTTGTTCGGGATGTTTCACTTCATCAATTAGAGTTAGTGCATCATTAAGACCAAGGTAATTCTGTGTCGAATAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACTAACATTTGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTGATACCAAGACCTa                                                                                                                                                                                                          <  2:219725/250‑1 (MQ=255)
ttCGGTTCAAGATCTTGTTCGGGATGTTTCACTTCATCAATTAGAGTTAGTGCATCATTAAGACCAAGGTAATTCTGTGTCGAATAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTa                                                                                                                                                                                                          >  1:227051/1‑250 (MQ=255)
       cAAGATCTTGTTCGGGATGTTTCACTTCATCAATTAGAGTTAGTGCATCATTAAGACCAAGGTAATTCTGTGTCGAATAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAAcc                                                                                                                                                                                                   >  1:85650/1‑250 (MQ=255)
              ttGTTCGGGATGTTTCACTTCATCAATTAGAGTTAGTGCATCATTAAGACCAAGGTAATTCTGTGTCGAATAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGtat                                                                                                                                                                                           >  2:125856/1‑251 (MQ=255)
               tGTTCGGGATGTTTCACTTCATCAATTAGAGTTAGTGCATCATTAAGACCAAGGTAATTCTGTGTCGAATAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGtat                                                                                                                                                                                           >  2:96021/1‑250 (MQ=255)
                   cGGGATGTTTCACTTCATCAATTAGAGTTAGTGCATCATTAAGACCAAGGTAATTCTGTGTCGAATAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGtta                                                                                                                                                                                      <  1:96021/251‑1 (MQ=255)
                                       aTTAGAGTTAGTGCATCATTAAGACCAAGGTAATTCTGTGTCGAATAGCGATAGGCCGCGATATTAAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATAcc                                                                                                                                                                  >  1:42489/1‑251 (MQ=255)
                                         tAGAGTTAGTGCATCATTAAGACCAAGGTAATTCTGTGTCGAATAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCATCGGTATAGTTATTATCGGTTATCTGAATACCgg                                                                                                                                                                >  2:223120/1‑251 (MQ=255)
                                                                       aTTCTGTGTCGAATAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCAGGCCgt                                                                                                                                  >  1:255364/1‑251 (MQ=255)
                                                                                    tAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCAGGCCGTAGTAGTAgcttgc                                                                                                                     >  2:108086/1‑251 (MQ=255)
                                                                                              ccGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGGTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCAGCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTTCTTGaaataaa                                                                                                           <  2:28443/251‑1 (MQ=255)
                                                                                                           tGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATCCt                                                                                              >  1:204633/1‑251 (MQ=255)
                                                                                                                    tGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATCCTGAATatcat                                                                                     >  2:275938/1‑251 (MQ=255)
                                                                                                                      tttCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATCCTGAATATCATCt                                                                                   >  1:140854/1‑251 (MQ=255)
                                                                                                                            cGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGGCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATCCTGAATATCATCTTTTaa                                                                              <  1:269861/250‑1 (MQ=255)
                                                                                                                              aaTAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCAGCCGGAATATCATCTTTTAAGAc                                                                           <  1:77563/251‑1 (MQ=255)
                                                                                                                                                acacGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATCCTGAATATCATCTTTTAAGACCTGACAGCCGCTAATATc                                                         >  2:5376/1‑251 (MQ=255)
                                                                                                                                                                                           gAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGCTAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATCCTGAATATCATCTTTTAAGACCTGACAGCCGCTAATATCCCAACGTCCAACGCCAGGGCGTAACATTTGAACAACGGATg                >  1:44154/1‑249 (MQ=255)
                                                                                                                                                                                                       gAGTCACATCGAAAGAAAATGCACAAACTGAAGTATTTAGAACAAGACCTAACAAACCAGCGGTATAGTAATTATGGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCGGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTAACCCTGAATATAATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGTAACATTTGAACAACGGATGAGAAAGGTTGCgag  <  2:55941/251‑1 (MQ=255)
                                                                                                                                                                                                        aGTCACATCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACATAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGTAACATTTGAACAACGGATGAGAAAGGTTGCg    >  1:128699/1‑248 (MQ=255)
                                                                                                                                                                                                                                  cTGAAGTATTTAGACCAGGACCTAACAACCCAGCGGTCTAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCAGGCCGTAGTAGAAGCTTGCTTGAAATAAATTAGGCTCATCCTGAATATCATATTTTAAGCCATGACCGCCGCTAATATCCCAACGTCCaa                                              >  2:196364/1‑180 (MQ=255)
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                     
TTCGGTTCAAGATCTTGTTCGGGATGTTTCACTTCATCAATTAGAGTTAGTGCATCATTAAGACCAAGGTAATTCTGTGTCGAATAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTCAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGTAACATTTGAACAACGGATGAGAAAGGTTGCGAG  >  NC_000913/153946‑154395

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: