Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	85,203	C→T	P279L (CCG→CTG)	leuO →	DNA‑binding transcriptional dual regulator LeuO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	85,203	0	C	T	100.0%	72.6 / NA	25	P279L (CCG→CTG)	leuO	DNA‑binding transcriptional dual regulator LeuO
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/18);  total (7/18)

GCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATCGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGCCGTTAAAACAAAACAGCAGAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTGTGGGCTCTCTATTTTAGGATTAATTAAAAAAATAGAGAAATTGCTGTA  >  NC_000913/84963‑85440                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                              gCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATCGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGGGTCGCAAACGCCTTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGCTGTTaaaaca                                                                                                                                                                                                                                      >  1:268545/1‑250 (MQ=255)       tACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATCGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGCTGTTAAAACAAAAcag                                                                                                                                                                                                                                <  1:259240/250‑1 (MQ=255)           gAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATCGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGCTGTTAAAACAAAACAGCAGaa                                                                                                                                                                                                                           <  2:6738/251‑1 (MQ=255)                            aCGAACAACATGAGGAGGTTTCGCTCGATCGTTTCGCGTCCTTTAGTCAACCCTGGTATGGCACGGTAGATCAGCAAACCAGTATCTCGTATCAGGGCATGGGAATGATGAGAGTAATTAGCGTGGGGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGGTCGCTGAATCCTTAGAATTAACGGTATTACCGCTGTTGTTAAAACAAAACAGCAGAACCGGTTATCACCCCTgg                                                                                                                                                                                                          >  2:207908/1‑251 (MQ=255)                                acaacaTGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATCGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGCTGTTAAAACAAAACAGCAGAACCTGTTATCTCTCCTGGCATg                                                                                                                                                                                                      >  1:128508/1‑251 (MQ=255)                                             gTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATCGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGCTGTTAAAACAAAACAGCAGAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGgcg                                                                                                                                                                                         >  1:102031/1‑251 (MQ=255)                                                  gCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATCGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGCTGTTAAAACAAAACAGCAGAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATa                                                                                                                                                                                    <  1:53257/251‑1 (MQ=255)                                                       aTCGTTGCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATCGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGCTGTTAAAACAAAACAGCAGAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGc                                                                                                                                                                               <  1:3208/251‑1 (MQ=255)                                                              cgcgTCATTTAGTCAACCTGGGTATGACACGGTAGATAAGCAAGCCAGTATCGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGCTGTTAAAACAAAACAGCAGAACCTGTTATCGCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGt                                                                                                                                                                        <  2:57128/251‑1 (MQ=255)                                                                      ttAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATCGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGCTGTTAAAACAAAACAGCAGAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGtggatgga                                                                                                                                                                 <  2:268545/250‑1 (MQ=255)                                                                         gTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATCGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGCTGTTAAAACAAAACAGCAGAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAagag                                                                                                                                                             <  1:150491/251‑1 (MQ=255)                                                                                     aTGACACGGTAGATAAGCAAGCCAGTAGCGCGTATCAGGGCATGGCAATGATGAGCGTACGTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTTGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCGGCTGTTAAAACAAAACAGCAGAACCTGGTATCTCGCCGGGCATTAAGCTGCCGGGCGCTATAAAGGCCAGCAGTGGAGGTAAGAGCAATTAGtctc                                                                                                                                                  <  2:309884/250‑1 (MQ=255)                                                                                        acacGGTAGATAAGCAAGCCAGTATCGCGTATCAGGGCATGGCAATGATGAGCGTTCTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATGACCGCTGCTGTTAAAACAAAACAGCAGAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGATCAATTAGTCTCAAtt                                                                                                                                              <  2:203079/251‑1 (MQ=255)                                                                                         cacGGTAGATAAGCAAGCCAGTATCGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGCTGTTAAAACAAAACAGCAGAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAAttt                                                                                                                                             >  1:250251/1‑251 (MQ=255)                                                                                                                         aGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGCTGTTAAAACAAAACAGCAGAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGa                                                                                                             <  2:102031/251‑1 (MQ=255)                                                                                                                                               aCTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGCTGTTAAAACAAAACAGCAGAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTg                                                                                       <  2:128508/251‑1 (MQ=255)                                                                                                                                                              gCAAACGCATTTGGTCGCTATGGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGCTGTGAAAACAAAACAGCAGAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCAGTTTTTATTGAAt                                                                        <  2:98854/251‑1 (MQ=255)                                                                                                                                                                       tttGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGCTGTTAAAACAAAACAGCAGAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAAttt                                                               >  1:211728/1‑251 (MQ=255)                                                                                                                                                                                             tggctggcTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGCTGTTAAAACAAAACAGCAGAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATATTTTATTGAATGTAGAATTTTATTCTGAATGTGTGGGctct                                          <  2:240295/250‑1 (MQ=255)                                                                                                                                                                                              ggctggctGAAGAGTTCGCTGAATCCTTAGAATTACAGGTTTTACCGCTGCTGTTAAAACAAAACAGCAGAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATGAGGCTCAATTTGCAAACGCTAACTGATTGTAGAATAGGTCAGACATGAATGTCTGTTTTATTCTGCATTTTGTATTGAAGGTAGAATTTTATTCTGAATGTGTTTGctctc                                         <  2:205299/250‑1 (MQ=255)                                                                                                                                                                                                           gTTCGCTGAATCCTTAGAATTACATGTATTACCGCTGCTGTTAAAACAAAACAGCAGAACCTGTTATCTCTCCTGGCATGAAGCTGCCTGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTTGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTGTGTGGTCTCTATGTTAGGattaa                           <  2:204553/251‑1 (MQ=255)                                                                                                                                                                                                                  gAATCCTTAGAATTACAGGTATGACCGCTGCTGTTAAAACAAAACAGCAGAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGTCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTGTGGGCTCTCTATTTTAGGATTAATTaaaaa                    <  1:14624/251‑1 (MQ=255)                                                                                                                                                                                                                   aaTCCTTAGCCTTACAGGTATCACCGCTGATGTTAACCCAAACCAGCAGAACCTGTTATCTCTCCTGGCCTGCAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGCAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTGTGGGCTCTCTATTTTAGGATTAATTaaaaa                    >  2:148707/1‑250 (MQ=255)                                                                                                                                                                                                                             aTTACAGGTATTACCGCTGCTGTTAAAACAAAACAGCAGAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTGTGGGCTCTCTATTTTAGGATTAATTAAAAAAATAGAGAAAt         <  1:238499/251‑1 (MQ=255)                                                                                                                                                                                                                                    gTATTACCGCTGCTGTTAAAACAAAACAGCAGAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTGTGGGCTCTCTATTTTAGGATTAATTAAAAAAATAGAGAAATTGCTGTa  <  1:56237/251‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                              GCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATCGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGCCGTTAAAACAAAACAGCAGAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTGTGGGCTCTCTATTTTAGGATTAATTAAAAAAATAGAGAAATTGCTGTA  >  NC_000913/84963‑85440

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑