Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	472,849	A→G	G84G (GGA→GGG)	glnK →	nitrogen regulatory protein PII‑2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	472,849	0	A	G	100.0%	150.1 / NA	42	G84G (GGA→GGG)	glnK	nitrogen regulatory protein PII‑2
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/17);  total (25/17)

TTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAGCGACGATAAAAACTGGGCTTGCTTCACTGGCGATGCTTCCGGGACTGGTAATGGCTGCACCTGCGGTGGCCGATAAAGCCGACAATGCGTTTATGATGATTTGTACTGCGCTG  >  NC_000913/472628‑473088                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                ttCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGc                                                                                                                                                                                                                    >  2:36975/1‑251 (MQ=255)    aaGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTg                                                                                                                                                                                                                  >  1:84574/1‑250 (MQ=255)    aaGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTg                                                                                                                                                                                                                  >  2:77830/1‑250 (MQ=255)     aGCTGGAAGACGTTCGTGAAGCGTTATCGTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGaa                                                                                                                                                                                                                <  1:154662/251‑1 (MQ=255)         ggAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGACAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACAAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACAGGGAAAATTGGCGACGGCAAAAt                                                                                                                                                                                                                             >  2:157302/1‑234 (MQ=255)         ggAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAAt                                                                                                                                                                                                                             <  1:157302/234‑1 (MQ=255)            aGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAAc                                                                                                                                                                                                         >  1:54356/1‑251 (MQ=255)                                   ccATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGc                                                                                                                                                                                  <  1:319736/251‑1 (MQ=255)                                        ggTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGc                                                                                                                                                                             >  1:39794/1‑251 (MQ=255)                                               aaggCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGGGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATATTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCcgacga                                                                                                                                                                       >  2:232059/3‑250 (MQ=255)                                                        ccGTCACCGAAGGGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCTGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATTGGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATAGCGTCAGTAAGGCGTCTTACACCGGGAAAATTGGCGGCGGCAAAATCTTCGTCGCTGAATTGCTACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTg                                                                                                                                                             <  1:303278/251‑1 (MQ=255)                                                                        aGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGcacaca                                                                                                                                             <  2:24809/251‑1 (MQ=255)                                                                             tCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGa                                                                                                                                                                                >  1:300955/1‑211 (MQ=255)                                                                             tCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCAGTCGTATGCGTACCGGCGa                                                                                                                                                                                <  2:300955/211‑1 (MQ=255)                                                                                                 gCCGAGCTGTACCGGGTGGCGGCATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGAt                                                                                                                     >  1:284913/1‑250 (MQ=255)                                                                                                       cTGTACCGGGAGTCGGAATACAGCGTCAATTTCCTGACAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGACGGGATCGATATCGTCAGTAAGGCTGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGGCGTTGCATTGCAACGCGTCACTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTATTCTCTGGCACACAGCAACAGCAACGAAAAATGAAGTTAgagccg                                                                                                              >  2:120133/1‑246 (MQ=255)                                                                                                               gggggCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGCAAAATGAAGATAGCGGCGATAAAAAc                                                                                                      >  2:89010/1‑251 (MQ=255)                                                                                                                                 cAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACg                                                                                                                                                                                                                                    >  2:243087/1‑107 (MQ=255)                                                                                                                                 cAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACg                                                                                                                                                                                                                                    <  1:243087/107‑1 (MQ=255)                                                                                                                                  aaTTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAGCGACGATAAAAACTGGGCTTGCTTCACTGGCg                                                                                   >  1:270571/1‑251 (MQ=255)                                                                                                                                       ccTGCCAAAAGTAAAAAGTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAGCGACGATAAAAACTGGGCTTGCTTCACTGGCGATGc                                                                               <  1:272892/250‑1 (MQ=255)                                                                                                                                             aaaaGTAAAAATTGATGTGGCGATTGCTGCTGACCAACTCGATGAAGAGATCGATATCGTCAGTAAGGAGGCTTACACCGGGCAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAGCGAAGATAAAAACTGGGCTTGCTTCACTGGCGATACTTCCggg                                                                        >  2:265035/1‑251 (MQ=255)                                                                                                                                             aaaaGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGAGACGGCAAAATCTTCGTCGCTGGATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAGCGACGATAAAAACTGGGCTTGCTTCACTGGCGATGCTTCCggg                                                                        >  1:131705/1‑251 (MQ=255)                                                                                                                                              aaaGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTTGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAGCGACGATAAAAACTGGGCTTGCTTCACTGGCGATGCTTCCggg                                                                        >  1:176953/1‑250 (MQ=255)                                                                                                                                               aaGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGACGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAGCGACGATAAAAACTGGGCTTGCTTCACTGGCGATGCTGCCGGGAc                                                                      >  2:95459/1‑251 (MQ=255)                                                                                                                                                    aaaaTTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAGCGACGATAAAAACTGGGCTTGCTTCACTGGCGATGCTTCCGGGACTGGTa                                                                 <  1:241123/251‑1 (MQ=255)                                                                                                                                                              tgGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAGCGACGATAAAAACTGGGCTTGCTTCACTGGCGATGCTTCCGGGACTGGTAATGGCTGCAc                                                       >  1:113582/1‑251 (MQ=255)                                                                                                                                                               ggCGATTGCTGCTGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGCCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACCGCAACAGGAACGAAAAATGAAGATAGCGACGATAAAAACTGGGCTTGCTTCACTGGCGATGCTTCCGGGACTGGTACTGGCTGCAcc                                                      >  1:316870/1‑251 (MQ=255)                                                                                                                                                                         gatgaCCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTCCACCGGGAACATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAGCGACGATAAAAACTGGGCTTGCTTCACTGGCGATGCTTCCGGGACTGGTAATGGCTGCACCTGCGGTGGcc                                            >  2:128908/1‑251 (MQ=255)                                                                                                                                                                                aaCTCGATGACGTGATCGATATCGTCCGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAACTGAAGACAGCGACGATAAAAACTGGGCTTGCTTCACTGGAGATGCTTCCGGGACTGGTAATGGCTGCACCTGCGGTGGCCGTTaa                                       >  2:32013/1‑249 (MQ=255)                                                                                                                                                                                  cTCGATGAAGTGATCGATCTCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAGCGACGATAAAACCTGGGCTTGCTTCACTGGCGATGCTTCCGGGACTGGTAATGGCTGCACCTGCGGCGGCCGATAAAGcc                                   >  1:244781/1‑251 (MQ=255)                                                                                                                                                                                        gAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAGCGACGATAAAAACTGGGCTTGCTTCACTGGCGATGCTTCCGGGACTGGTAATGGCTGCACCTGCGGTGGCCGATAAAGCCGACAAt                             >  1:75700/1‑251 (MQ=255)                                                                                                                                                                                            ggATCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAGCGACGATAAAAACTGGGCTTGCTTCACTGGCGATGCTTCCGGGACTGGTAATGGCTGCACCTGCGGTGGCCGATAAAGCCGACAATGCGt                         <  2:54356/250‑1 (MQ=255)                                                                                                                                                                                              aTCGATATCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAGCGACGATAAAAACTGGGCTTGCTTCACTGGCGATGCTTCCGGGACTGGTAATGGCTGCACCTGCGGTGGCCGATCAAGCCGACAATGCGttt                       >  2:47695/1‑251 (MQ=255)                                                                                                                                                                                                cGATATCGTCAGTAAGGCGGGTTATACCGTGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGTTAGCGATTATAAAAACTGGGCTTGCTGCACTGGCGATGCTTCCGGGACTGGTAATGGCTGCCCCTGCGGTAGCCGATAAAGCCGACAATGGGTTTa                      <  2:316881/250‑1 (MQ=255)                                                                                                                                                                                                    atCGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAGCGACGATAAAAACTGGGCTTGCTTCACTGGCGATGCTTCCGGGACTGGTAATGGCTGCACCTGCGGTGGCCGATAAAGCCGACAATGCGTTTatgat                  <  1:47695/250‑1 (MQ=255)                                                                                                                                                                                                      cGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGc                                                                                                                                                  >  2:92480/1‑120 (MQ=255)                                                                                                                                                                                                      cGTCAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGc                                                                                                                                                  <  1:92480/120‑1 (MQ=255)                                                                                                                                                                                                         cAGTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAGCGACGATAAAAACTGGGCTTGCTTCACTGGCGATGCTTCCGGGACTGGTAATGGCTGCACCTGCGGTGGCCGATAAAGCCGACAATGCGTTTATGATGATTTg            <  2:75700/251‑1 (MQ=255)                                                                                                                                                                                                           gTAAGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAGCGACGATAAAAACTGGGCTTGCTTCACTGGCGATGCTTCCGGGACTGGTAATGGCTGCACCTGCGGTGGCCGATAAAGCCGACAATGCGTTTATGATGATTTGTa          <  1:36975/251‑1 (MQ=255)                                                                                                                                                                                                              aGGCGGCTTACACCGGGAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAGCGACGATAAAAACTGGGCTTGCTTCACTGGCGATGCTTCCGGGACTGGTAATGGCTGCACCTGCGGTGGCCGATAAAGCCGACAATGCGTTTATGATGATTTGTACTg       <  1:178071/251‑1 (MQ=255)                                                                                                                                                                                                                   gcTTACACCGGGAAACTTGGCGACGGCAAAATCTGCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGAGAGCGTCGATAAAAACTGGGCTTGCTTCACTGGCGATGCTTCCGGGACTGGTAATGGCTGCACCTGCGGTGGCCGATAAAGCCGACAATGCGTTTATGATGATTTGTACTGCGCTg  <  2:256438/251‑1 (MQ=255)                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                TTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAGCGACGATAAAAACTGGGCTTGCTTCACTGGCGATGCTTCCGGGACTGGTAATGGCTGCACCTGCGGTGGCCGATAAAGCCGACAATGCGTTTATGATGATTTGTACTGCGCTG  >  NC_000913/472628‑473088

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑