Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	2,094,553	IS1 (+) +9 bp	coding (19‑27/591 nt)	hisH →	imidazole glycerol phosphate synthase subunit HisH

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	1978503 =	NA (NA)	31 (0.960)	30/488	0.2	100%	noncoding (768/768 nt)	IS1	repeat region
	?	NC_000913	2094553 =	0 (0.000)					coding (19/591 nt)	hisH	imidazole glycerol phosphate synthase subunit HisH
*	?	NC_000913	= 1979270	NA (NA)	24 (0.740)	24/488	0.4	100%	noncoding (1/768 nt)	IS1	repeat region
	?	NC_000913	= 2094561	0 (0.000)					coding (27/591 nt)	hisH	imidazole glycerol phosphate synthase subunit HisH

CGCCATTCATGGCCATATCAATGATTTTCTGGTGCGTACCGGGTTGAGAAGCGGTGTAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/1979026‑1979270 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GCCGGTATCAAGGATCACCACGTTCATTACAGCACTCCTTTCGACGAGGGCAGGGTATCGCCTTCCACGCGGATGGCCTGGCGCAGGGTGCGACCAAAGGCTTTGAACAGACTCTCTACACGGTGATGATCGTTTTTACCTTTGGTTTTCAGGTGTAGCGTCACGCCCATGGTGTATGAGAGCGAACGGAAGAAGTGCTCGATCATTTCGGTGCTGAGATCGCCCACGCGCTGGTAGGTAAACTCGGCTT  <  NC_000913/2094561‑2094312                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 CGCCATTCATGGCCATATCAATGATTTTCTGGTGCGTACCGGGTTAAGAAGCGGTGCAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAACCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGGAAGTTGGCAGCATCACCGCCGGg                                                                                                                                                                                                                                                      >  2:310462/1‑250              CATATCAATGATTTTATAGTGCGTACCGGCTTGAGGAGCGGTGTACGAGAACTGCAGTTGCCATGTTTTACGGCAGTGCGAGCAGGGATAGCGCTGATGGCAGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGCACAGGAGGGACAGCTGATAGAACAAGAAGCCCCTGGAGCCCCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACAGCCGGTATCAAGGATCccc                                                                                                                                                                                                                                         >  2:48427/1‑248                      TGATTTTCTGGTGCGTACCGGGTTGAGAAGCGGTGGAAGTGAACTGCAGGTGACATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGGCGGTGCTTTTGCCGGTACGCACCACCCAGTCAGGAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAAAAACATCATACACTAAATAAGTAAGTTGGCAGCATCACCGCAGGTATCAAGGATCACCACGggcat                                                                                                                                                                                                                                 >  2:118560/1‑246                                     TACCGGGTTGAGAAGCGGTGTAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGCCGGTATCAAGGATCACCACGTTCATTACAGCACTCCTTTC                                                                                                                                                                                                                  >  1:294569/1‑251                                        CGGGTTGAGAAGCGGTGTAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGCCGGTATCAAGGATCACCACGTTCATTACAGCACTCCTTTCGAC                                                                                                                                                                                                               >  1:278641/1‑251                                                    CGGTGTAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTAAGCACCACCCCGTCAGTAGCTGCACAGGCGGGACAGCTGATAGAAACAGAAGCCACTGGAGAACCTCAAACACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGCCGGTATCAAGGATCACCACGTTCATTACAGCACTCCTTTCGACGAGGGCAGGGT                                                                                                                                                                                                    >  1:322208/1‑250                                                                                              AGCAGAGATAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGCCGGTATCAAGGATCACCACGTTCATTACAGCACTCCTTTCGACGAGGGCAGGGTATCGCCTTCCACGCGGATGGCCTGGCGCAGGGTGCGACCAA                                                                                                                                                           >  1:201237/1‑249                                                                                                       AGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGCCGGTATCAAGGATCACCACGTTCATTACAGCA                                                                                                                                                                                                                          >  2:87940/1‑177                                                                                                         CGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGCCGGTATCAAGGATCACCACGTTCATTACAGCACTCCTTTCGACGAGGGCAGGGTATCGCCTTCCACGCGGATGGCCTGGCGCAGGGTGCGACCAAAGGCTTTGAAC                                                                                                                                                >  2:245319/1‑249                                                                                                          GCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCACGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGCCGGTATCAAGGATAACCACGTTCATTACAGCACTCCTTTCGACGAGGGCAGGGAATCGCCTTCCACGCGGATGGCCTGGCGCAGGGTGCGACCAAAGGCTTTGAcc                                                                                                                                                >  2:181632/1‑246                                                                                                                  CCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGTCAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTACGTTGGCAGCATCACCGCCGGTATCAAGGATCACCACGTTCATTACAGCACTCCTTTCGACGAGGGCAGGGTATCGCCTTCCACGCGGATGGCCTGGCGCAGGGCGCGACCACAGGCTTTGAACAGACTCTCTAC                                                                                                                                     >  2:274695/1‑251                                                                                                                               TGCCGTTACTCCCCCCCCCGCCAGTAGCTAAACAGGGGGAAAAGCTGAAAAAAACAGAACCCACTGGACCACCTAAAAAACCCAATCATACACTAAATCAGTAAGTTGGCAGCATCACCGCCGGTATCAAGGATCACCACGTTCATTACAGCACTCCTTTCGACGAGGGCAGGGTATCGCCTTCCACGCGGATGGCCTGGCGCAGGGTGCGACCAAAGGCTTTGAACAGACTCTCTACACGGTGATGATC                                                                                                                         <  1:201846/250‑1                                                                                                                                                       TAGCTGAACAGGAGGGACAGATGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATAAACTAAATCAGTAAGTTGGCAGCATCACCGCCGGTATCAAGGATCACCACGTTCATTACAGCCCTCCTTTCGACGAGGGCAGGGTATCGCCTTCCACGCGGCTGGCCTGGCGCAGGGTGCGACCACAGGCTTTGAACAGACTCTCTACACGGTGATGATCGTTTTTACCTTTGGTTTTCAGGTGT                                                                                                >  1:116026/1‑251                                                                                                                                                                       ACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGCCGGTATCAAGGATCACCACGTTCATTACAGCACTCCTTTCGACGAGGGCAGGGTATCGCCTTCCACGCGGATGGCCTGGCGCAGGGTGCGACCAAAGGCTTTGAACAGACTCTCTACACGGTGATGATCGTTTTTACCTTTGGTTTTCAGGTGTAGCGTCACGCCCATGG                                                                                >  2:5644/1‑251                                                                                                                                                                                     CAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGCCGGTATCAAGGATCACCACGTTCATTACAGCACTCCTTTCGACGAGGGCAGGGTATCGCCTTCCACGCGGATGGCCTGGCGCAGGGTGCGACCAAAGGCTTTGAACAGACTCTCTACACGGTGATGATCGTTTTTACCTTTGGTTTTCAGGTGTAGCGTCACGCCCATGGTGTATGAGAGCGA                                                                   >  2:164653/1‑250                                                                                                                                                                                                         aaaaaccccccTCATACACTAAACAAGAAAGTTGGCAGCATCTCCGCCGGTATCAAGGATCACCACGTTCATTACAGCACTCCTTTCGACGAGGGCAGGGTATCGCCTTCCACGCGGATGGCCTGGCGCAGGTTGCGACCAAAGGCTTTGAACAGACTCTCTACACGGGGATGATCGTTTTTACCTTTGGTTTTCAGGTGTAGCGTCACGCCCATGGTGTATGAGAGCGAACGGAAGAAGTGCTCGATCAT                                              <  1:310462/240‑1                                                                                                                                                                                                                   ATCATACACTAAATCAGTAAGTTGGCAGCATCACCGCCGGTATCAAGGATCACCACGTTCATTACAGCACTCCTTTCGACGAGGGCAGGGTATCGCCTTCCACGCGGATGGCCTGGCGCAGGGTGCGACCAAAGGCTTTGAACAGACTCTCTACACGGTGATGATCGTTTTTACCTTTGGTTTTCAGGTGTAGCGTCACGCCCATGGTGTATGAGAGCGAACGGAAGAAGTGCTCGATCATTTCGGTGCTG                                    >  1:317425/1‑251                                                                                                                                                                                                                              AATCAGTAAGTTGGCAGCATCACCGCCGGTATCAAGGATCACCACGTTCATTACAGCACTCCTTTCGACGAGGGCAGGGTATCGCCTTCCACGCGGATGGCCTGGCGCAGGGTGCGACCAAAGGCTTTGAACAGACTCTCTACACGGTGATGATCGTTTTTACCTTTGGTTTTCAGGTGTAGCGTCACGCCCATGGTGTATGAGAGCGAACGGAAGAAGTGCTCGATCATTTCGGTGCTGAGATCGCCCAC                         >  1:142155/1‑251                                                                                                                                                                                                                                    TAAGTTGGCAGCATCACCGCCGGTATCAAGGATCACCACGTTCATTACAGCACTCCTTTCGACGAGGGCAGGGTATCGCCTTCCACGCGGATGGCCTGGCGCAGGGTGCGACCAAAGGCTTTGAACAGACTCTCTACACGGTGATGATCGTTTTTACCTTTGGTTTTCAGGTGTAGCGTCACGCCCATGGTGTATGAGAGCGAACGGAAGAAGTGCTCGATCATTTCGGTGCTGAGATCGCCCACGCGCTG                   >  2:97507/1‑251                                                                                                                                                                                                                                       gtgGGCAGCCTCACCGCCGGTATCAAGGATCACCACGTTCATTACAGCACTCCTTTCGACGAGGGCAGGGTATCGCCTTCCACGCGGATGGCCTGGCGCAGGGTGCGACCAAAGGCTTTGAACAGACTCTCTACACGGTGATGATCGTTTTTACCTTTGGTTTTCAGGTGTAGCGTCACGCCCATGGTGTATGAGAGCGAACGGAAGAAGTGCTCGATCATTTCGGTGCTGAGATCGCCCACGCGCTGGTA                <  2:278641/248‑1                                                                                                                                                                                                                                         gGGCAGCCTCGCCGCCGGTATCAAGGATCACCACGTTCATTACAGCACTCCTTGCGACGAGGGCAGGGTATCGCCTTCCACGCGGATGGCCTGGCGCAGGGTGCGACCAAAGGCTTTGAACATACTCTCTACACGGTGATGATCGTTTTTACCTTTGGTTTTCAGGTGTAGCGTCACGCCCATGGTGTATGAGAGCGAACGGAAGAAGTGCTCGATCATTGCGGTGCTGAGATCGCCCACGCGCTGGTAG               <  1:274695/249‑1                                                                                                                                                                                                                                            ccgctTCACCGCCGGTATCAAGGATCACCACGTTCATTACAGCTCGCCTTTCGTCGCGGGCAGGGTATCGCCTTCCACGCGGATGGCCTGGCGCAGTGTGCGACCAAAGGCTTTGAACAGACTCTCTACACGGTGATGATCGTTTTTACCTTTGGTTTTCAGGTGTAGCGTCACGCCCATGGTGTATGAGAGCGAACGGAAGAAGTGCTCGATCATTGCGGTGCTGAGATCTTCCACGCGCTGGTAGGTAA           <  2:105501/246‑1                                                                                                                                                                                                                                              GCATCACCGCCGGTATCAAGGATCACCACGTTCATTACAGCACTCCTTTCGACGAGGGCAGGGTATCGCCTTCCACGCGGATGGCCTGGCGCAGGGTGCGACCAAAGGCTTTGAACAGACTCTCTACACGGTGATGATCGTTTTTACCTTTGGTTTTCAGGTGTAGCGTCACGCCCATGGTGTATGAGAGCGAACGGAAGAAGTGCTCGATCATTTCGGTGCTGAGATCGCCCACGCGCTGGTAGGTAAAC         >  2:107139/1‑251                                                                                                                                                                                                                                                     CGCCGGTATCAAGGATCACCACGTTCATTACAGCACTCCTTTCGACGAGGGCAGGGTATCGCCTTCCACGCGGATGGCCTGGCGCAGGGTGCGACCAAAGGCTTTGAACAGACTCTCTACACGGTTATTATCGTTTTTACCTTTGGTTTTCAGGTGTAGCGTCGCGCCCATGGTGTGTGAGAGCGAACGGAGGAAGTGCGCGATTATTTCGGTGCTGAGATCGCCCACGCGCTGGTAGGTAAACTCGGCTT  <  1:48427/251‑1                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 CGCCATTCATGGCCATATCAATGATTTTCTGGTGCGTACCGGGTTGAGAAGCGGTGTAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/1979026‑1979270 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GCCGGTATCAAGGATCACCACGTTCATTACAGCACTCCTTTCGACGAGGGCAGGGTATCGCCTTCCACGCGGATGGCCTGGCGCAGGGTGCGACCAAAGGCTTTGAACAGACTCTCTACACGGTGATGATCGTTTTTACCTTTGGTTTTCAGGTGTAGCGTCACGCCCATGGTGTATGAGAGCGAACGGAAGAAGTGCTCGATCATTTCGGTGCTGAGATCGCCCACGCGCTGGTAGGTAAACTCGGCTT  <  NC_000913/2094561‑2094312

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.