Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,748,841	C→T	R240K (AGA→AAA)	ydhT ←	uncharacterized protein YdhT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,748,841	0	C	T	96.0%	73.3 / NA	26	R240K (AGA→AAA)	ydhT	uncharacterized protein YdhT
Reads supporting (aligned to +/- strand):  ref base C (0/1);  major base T (12/12);  minor base G (0/1);  total (12/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCTGGTTGATGCACGACCAGCCTTTCGTTCAGGGACTTACGGCATGTGCAGAAATTGGTGAGGCGATGGCTCGGGCGGTCGTAAAGCCTGCATCCCGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTT‑TCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTCCGGTATTCGCAATCCG  >  NC_000913/1748611‑1749085                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      ccTGGTTGATGCACGACCAGCCTTTCGTTCAGGGACTTACGGCAGGTGCAGAAATTGGTGAGGCGATGGCGCGGGCGGTCGTAAAGCCTGCATCCCGTGCTCGGCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTAAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTGTTCACCTTCAATGGCGACGGCGATGCGAACTTTT‑TCGCCGTCATGATATGcc                                                                                                                                                                                                                                  <  2:80113/251‑1 (MQ=255)        gATGCACGACCAGCCTTTCGTTCAGGGACTTACGGCATGTGCAGAAATTGGTGAGGCGATGGCTCGGGCGGTCGTAAAGCCTGCATCCCGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCGGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTT‑TCGCCGTCATGATATGCCAGTgcgg                                                                                                                                                                                                                           <  2:114505/251‑1 (MQ=255)               gACCAGCCTTTCGTTCAGGGACTTACGGCATGTGCAGAAATTGGTGAGGCGATGGCTCGGGCGGTCGTTAAGCCTGCATCCCGTGCTCGTCGGCGTCTTTCGTTTGATTCAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCAGACTCCGGCTCGGATATTGTTTTCACCTTCAATGGCGGAGGCGAAACGAACTTTT‑TCGCCGTCATGCTATGCAAGTGCGGCGCTtcc                                                                                                                                                                                                                    >  1:140578/1‑251 (MQ=255)                 ccAGCCTTTCGTTCAGGGACTTACGGCATGTGCAGAAATTGGTGAGGCGATGGCTCGGGCGGTCGTAAAGCCTGCATCCCGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTT‑TCGCCGTCATGATATGCCAGTGCGGCGCTtcctc                                                                                                                                                                                                                  <  1:244976/251‑1 (MQ=255)                   aGCCTGTCGTTCAGGGACTTACGGCATGTGCAGAAATTGGTGAGGCGATGGCTCGGGCGGTCGTAAAGCCTGCATCCCGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACGCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTT‑TCGCCGTCATGATATGCCAGTGCGGCGCTTcctcct                                                                                                                                                                                                                <  1:21306/251‑1 (MQ=255)                             tCAGGGACTTACGGCAGGTGCAGAAATTGGTGAGGCGATGGCTCGGGCGGTCGTAAAGCCTGCATCCCGTGCTCGTCGGCGTCTTTCGGTTGATGAAAGGTAAAGCTATCAGCACTCAACAAACTGAGGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTGCACCTTCAATGGCGACGGCGAAGCGAGCTTTT‑TCGGCGGCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCgaga                                                                                                                                                                                                       <  2:291711/250‑1 (MQ=255)                                gggACTTACGGCATGTACAGAAATTGGTGAGGCGATGGCTCGGGCGGTCGTAAAGCCTGCATCCCGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGCAAAGCTATCAGCAATCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCCCGCATATTGTTTTCCCCTTCAATGGCCACGGCGAAGCGAACTTTT‑TCGCCGTCATGATATGCCAGGGCGGCCCTTCCTCCTGGCCCGGGaata                                                                                                                                                                                                    >  2:160342/1‑250 (MQ=255)                                         ggCATGTGCAGAAATTGGTGAGGCGATGGCTCGGGCGGTCGTAAAGCCTGCATCCAGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTT‑TCGCCGTCATGATATGCCAGAGCGGCGCTTCCTCCTTTCCCGCGAATAATTGAGTCg                                                                                                                                                                                           >  2:212091/1‑250 (MQ=255)                                                acAGAAATTGGTGAGGCGATGGCTCGGGCGGTCGTAAAGCCTGCATCCCGTGATCGTCGGCGTCTTTCGTTTGCTTAAAGGGAAAGATATCAGCACTCATCAACCGGATGCTGTTCATTAAGACGATAGCGGACTCCTGCTAGAATATTGTTTTCACCTTCAATGGCGACCGCGAAGCGAACTTTT‑TCGCCGTCTTGATATGCAAGTACTGCGCTTCCTCCTGGCCCGAGAATAAATGAGTAGTCCACgac                                                                                                                                                                                   >  2:244959/2‑249 (MQ=255)                                                                          ggCGGTCGTAAAGCCTGCATCCCGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTT‑TCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTc                                                                                                                                                           >  1:293200/1‑249 (MQ=255)                                                                          ggCGGTCGTAAAGCCTGCATCCCGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTT‑TCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTc                                                                                                                                                           <  2:293200/249‑1 (MQ=255)                                                                          ggCGGTCGTAAAGCCTGCATCCCGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTT‑TCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCAc                                                                                                                                                         >  2:50930/1‑251 (MQ=255)                                                                                cGTAAAGCCTGCAGCCCGTGCTCGTCGGCGTCTTTCGTTTGAGTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTT‑TCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCTCCGCTgg                                                                                                                                                   <  2:184197/251‑1 (MQ=255)                                                                                     aGCCTGCCTCCCGTGCTCGTCGGCGTCTTTCGTTTGCTTCAAGGTAAAGCTATCAGCACTCAACAGACTGATGCTGTTCATTAAGACGATAGCGGACTCAGGCTCGAATATTGTTTTCACCTTCAATGCCGACGGCGAAGCGAACTTTT‑TCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATATTTGCGTCGACCACGCACGTGCCCACTACCGTTGCATTTTCACCGCTGGCactg                                                                                                                                              >  2:319057/1‑248 (MQ=255)                                                                                                     ccgtcgGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTT‑TCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTcgc                                                                                                                              <  1:50930/250‑1 (MQ=255)                                                                                                                                        aTCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTT‑TCGCCGt                                                                                                                                                                                                                                             >  2:225244/1‑105 (MQ=255)                                                                                                                                        aTCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTT‑TCGCCGt                                                                                                                                                                                                                                             <  1:225244/105‑1 (MQ=255)                                                                                                                                             cACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGAATCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTT‑TCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCgct                                                                                      >  2:236773/1‑249 (MQ=255)                                                                                                                                                       aCTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTTTT‑TCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAgg                                                                            <  2:78730/251‑1 (MQ=255)                                                                                                                                                                                                       tttCACCTTCAATGGCGACGGCGAAGCGAACTTTT‑TCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCTATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAAcgcg                             <  1:43355/250‑1 (MQ=255)                                                                                                                                                                                                             cTTCAATGGCGACGGCGAAGCGAACTTTT‑TCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGc                      >  1:232032/1‑251 (MQ=255)                                                                                                                                                                                                                gaagggggACGGTGAAGCGAACTGTG‑TGGCCGTCATGATATGCAAGTGCGGCGATTCCTCCTGGGCCGAGAATAATTGGGTCGACCACGCTCGTGCTCACGACCGTTGAATTTTCACCGCTGGCAATGATGGGTTCATTGTCGCCGCTGATGGCAATTCTGGCGTTTGGGCCAAAGCTGGCGATTTGCACCAGGTCTCCATTACTGGCGACATGACACCGTTCACCCAATGTTCGAACGCGTACTCGCAtt                   <  2:199300/244‑1 (MQ=255)                                                                                                                                                                                                                 aaTGGCGACGGCGAAGCGAACTTTT‑TCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTc                  >  2:213555/1‑251 (MQ=255)                                                                                                                                                                                                                    ggCGACGGCGAAGCGAAC‑CTTGTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCTCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTTCCGTTGAACTTTCACCGCTGGCAAGGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTGCTCGCATTCCg                <  1:164677/250‑1 (MQ=255)                                                                                                                                                                                                                              aagcgaaCTTTT‑TCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTCCGGTAGTCGCAAt     <  1:66962/251‑1 (MQ=255)                                                                                                                                                                                                                                 cgaaCTTTT‑TCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCAGAGAATAATTGAGTCGACCACGCCCGAGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTCCGGTATTCGCAATCCg  >  2:29470/1‑251 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      CCTGGTTGATGCACGACCAGCCTTTCGTTCAGGGACTTACGGCATGTGCAGAAATTGGTGAGGCGATGGCTCGGGCGGTCGTAAAGCCTGCATCCCGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTT‑TCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGCTGTTGGCAATTCTGGCGTTTGCGCCAAAGCTGGCGATTTGCACCAGGTCACCATTACTGGCGACATGACACCGTTCACCCAATGTACAAACGCGTACTCGCATTCCGGTATTCGCAATCCG  >  NC_000913/1748611‑1749085

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑