Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	456,711	C→G	P12R (CCC→CGC)	clpP →	ATP‑dependent Clp protease proteolytic subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	456,711	0	C	G	100.0%	124.0 / NA	36	P12R (CCC→CGC)	clpP	ATP‑dependent Clp protease proteolytic subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (14/22);  total (14/22)

GTGGGCTTTTTTTTGTCATGAATTTTGCATGGAACCGTGCGAAAAGCCTCTTTCGGTGTTAGCGTAACAACAAAAGATTGTTATGCTTGAAATATGGTGATGCCGTACCCATAACACAGGGACTAGCTGATAATCCGTCCATAAGGTTACAATCGGTACAGCAGGTTTTTTCAATTTTATCCAGGAGACGGAAATGTCATACAGCGGCGAACGAGATAACTTTGCACCCCATATGGCGCTGGTGCCGATGGTCATTGAACAGACCTCACGCGGTGAGCGCTCTTTTGATATCTATTCTCGTCTACTTAAGGAACGC‑GTCATTTTTCTGACTGGCCAGGTTGAAGACCACATGGCTAACCTGATTGTGGCGCAGATGCTGTTCCTGGAAGCGGAAAACCCAGAAAAAGATATCTATCTGTACATTAACTCCCCAGGCGGGGTGATCACTGCCGGGATGTCTATCTATGACACCAT  >  NC_000913/456484‑456957                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                        gtggGCTTTTTTTTGTCATGAATTTTGCATGGAACCGTGCGAAAAGCCTCTTTCGGTGTTAGCGTAACAACAAAAGATTGTTATGCTTGAAATATGGTGATGCCGTACCCATAACACAGGGACTAGCTGATAATCCGTCCATAAGGTTACAATCGGTACAGCAGGTTTTTTCAATTTTATCCAGGAGACGGAAATGTCATACAGCGGCGAACGAGATAACTTTGCACGCCATATGGCGCTGGTGCCGATgg                                                                                                                                                                                                                                  <  2:259492/251‑1 (MQ=255)          tttttGTCATGAATTTTGCATGGAACCGTGCGAAAAGCCTCTTTCGGTGTTAGCGTAACAACAAAAGATTGTTATGCTTGAAATATGGTGATGCCGTACCCATAACACAGGGACTAGCTTATAATCCGTCCATAAGGTTACAATCGGTACAGCAGGTTTTTTCAATTTTATCCAGGAGACGGAAATGTCATACAGCGGCGAACGAGATAACTTTGCACGCCATATGGCGCTGGTGCCGATGGTCATTGaa                                                                                                                                                                                                                          <  2:145242/250‑1 (MQ=255)              tGTCATGAATTTTGCATGGAACCGTGCGAAAAGCCTCTTTCGGTGTTAGCGTAACAACAAAAGATTGTTATGCTTGAAATATGGTGATGCCGTACCCATAACACAGGGACTAGCTGATAATCCGTCCATAAGGTTACAATCGGTACAGCAGGTTTTTTCAATTTTATCCAGGAGACGGAAATGTCATACAGCGGCGAACGAGATAACTTTGCACGCCATATGGCGCTGGTGCCGATGGTCATTGAACAGa                                                                                                                                                                                                                      <  1:21284/250‑1 (MQ=255)                                          aaaaGCCTCTTTCGGTGTTAGCGTAACAACAAAAGATTGTTATGCTTGAAATATGGTGATGCCGTACCCATAACACAGGGACTAGCTGATAATCCGTCCATAAGGTTACAATCGGTACAGCAGGTTTTTTCAATTTTATCCAGGAGACGGAAATGTCATACAGCGGCGAACGAGATAACTTTGCACGCCATATGGCGCTGGTGCCGATGGTCATTGAACAGACCTCACGCGGTGAGCGCTCTTTTGATATc                                                                                                                                                                                         <  2:61802/251‑1 (MQ=255)                                             aGCCTCTTTCGGTGTTAGCGTAACAACAAAAGATTGTTATGCTTGAAATATGGTGATGCCGTACCCATAACACAGGGACTAGCTGATAATCCGTCCATAAGGTTACAATCGGTACAGCAGGTTTTTTCAATTTTATCCAGGAGACGGAAATGTCATACAGCGGCGAACGAGATAACTTTGCACGCCATATGGCGCTGGTGCCGATGGTCATTGAACAGACCTCACGCGGTGAGCGCTCTTTTGTTATCTAt                                                                                                                                                                                      >  2:281951/1‑251 (MQ=255)                                                          gtTAGCGTAACAACAAAAGATTGTTATGCTTGAAATATGGTGATGCCGTACCCATAACACAGGGACTAGCTGATAATCCGTCCATAAGGTTACAATCGGTACAGCAGGTTTTTTCAATTTTATCCAGGAGACGGAAATGTCATACAGCGGCGAACGAGATAACTTTGCACGCCATATGGCGCTGGTGCCGATGGTCATTGAACAGACCTCACGCGGTGAGCGCTCTTTTGATATCTATTCTCGTCTACTTa                                                                                                                                                                         <  1:25249/251‑1 (MQ=255)                                                               cGTAACAACAAAAGATTGTTATGCTTGAAATATGGTGATGCCGTACCCATAACACAGGGACTAGCTGATAATCCGTCCATAAGGTTACAATCGGTACAGCAGGTTTTTTCAATTTTATCCAGGAGACGGAAATGTCATACAGCGGCGAACGAGATAAATTTGCACGCCATATGGCGCTGGTGCCGATGGTCATTGAACAGACCTCACGCGGTGAGCGCTCTTTTGATATCTATTCTCGTCTACTTAAGGa                                                                                                                                                                     >  1:62179/1‑250 (MQ=255)                                                                      acaaAAGATTGTTATGCTTGAAATATGGTGATGCCGTACCCATAACACAGGGACTAGCTGATAATCCGTCCATAAGGTTACAATCGGTACAGCAGGTTTTTTCAATTTTATCCAGGAGACGGAAATGTCATACAGCGGCGAACGAGATAACTTTGCACGCCATATGGCGCTGGTGCCGATGGTCATTGAACAGACCTCACGCGGTGAGCGCTCTTTTGATATCTATTCTCGTCTACTTAAGGAACGC‑GTc                                                                                                                                                             >  1:218077/1‑250 (MQ=255)                                                                        aaaaGATTGTTATGCTTGAAATATGGTGATGCCGTACCCATAACACAGGGACTAGCTGATAATCCGTCCATAAGGTTACAATCGGTACAGCAGGTTTTTTCAATTTTATCCAGGAGACGGAAATGTCATACAGCGGCGAACGAGATAACTTTGCACGCCATATGGCGCTGGTGCCGATGGTCATTGaa                                                                                                                                                                                                                          >  2:242608/1‑188 (MQ=255)                                                                        aaaaGATTGTTATGCTTGAAATATGGTGATGCCGTACCCATAACACAGGGACTAGCTGATAATCCGTCCATAAGGTTACAATCGGTACAGCAGGTTTTTTCAATTTTATCCAGGAGACGGAAATGTCATACAGCGGCGAACGAGATAACTTTGCACGCCATATGGCGCTGGTGCCGATGGTCATTGAACAGACCTCACGCGGTGAGCGCTCTTTTGATATCTATTCTCGTCTACTTAAGGAACGC‑GTCAtt                                                                                                                                                          >  1:34412/1‑251 (MQ=255)                                                                        aaaaGATTGTTATGCTTGAAATATGGTGATGCCGTACCCATAACACAGGGACTAGCTGATAATCCGTCCATAAGGTTACAATCGGTACAGCAGGTTTTTTCAATTTTATCCAGGAGACGGAAATGTCATACAGCGGCGAACGAGATAACTTTGCACGCCATATGGCGCTGGGGCCGATGGTCATTGaa                                                                                                                                                                                                                          <  1:242608/188‑1 (MQ=255)                                                                           aGATTGTTATGCTTGAAATATGGTGATGCCGTACCCATAACACAGGGACTAGCTGATAATCCGTCCATAAGGTTACAATCGGTACAGCAGGTTTTTTCAATTTTATCCAGGAGACGGAAATGTCATACAGCGGCGAACGAGATAACTTTGCACGCCATATGGCGCTGGTGCCGATGGTCATTGAACAGACCTCACGCGGTGAGCGCTCTTTTGATATCTATTCTCGTCTACTTAAGGAACGC‑GTCAttttt                                                                                                                                                       >  2:30028/1‑251 (MQ=255)                                                                               tGTTATGCTTGAAATATGGTGATGCCGTACCCATAACACAGGGACTAGCTGATAATCCGTCCATAAGGTTACAATCGGTACAGCAGGTTTTTTCAATTTTATCCAGGAGACGGAAATGTCATACAGCGGCGAACGAGATAACTTTGCACGCCATATGGCGCTGGTGCCGATGGTCATTGAACAGACCTCACGCGGTGAGCGCTCTTTTGATATCTATTCTCGTCTACTTAAGGAACGC‑GTCATTTTTCTg                                                                                                                                                    >  1:265715/1‑250 (MQ=255)                                                                                gTTATGCTTGAAATATGGTGATGCCGAACCCATAACACAGGGACTAGCTTATAATCCGTCCATAAGGTTACAATCGGTACAGCAGGTTTTTTCAATTTTATCCAGGAGACGGAAATGTCATACAGCGGCGAACGAGATAACTTTGCACGCCATATGGCGCTGGTGCCGATGGTCATTGAACAGACCTCACGCGGTGAGCGCTCTTTTGCTATCTATTCTCGTCTACTTAAGGACCGC‑GTCATTTTTCTGAc                                                                                                                                                  >  1:107494/1‑251 (MQ=255)                                                                                        tGAAATATGGTGTTGCCGTACCCATAACACAGGGACTAGCTGAGAATCCGTCCAGAAGGTTACAATCGGTACAGCAGGTTTTTGCAATTTTATCCATGAGACGGAAATGTGAGACATCGGCGAACGAGATAACTTTGCACGCCATATGGCGCTGGTGCCGATGGGCATTGAGCAGACCTCGCGGGGTGAGCGCTCTTTTTATGTCTATTCTCGTCTACTTAAGGAACGC‑GTCATTTTTCTGACTGGCCAg                                                                                                                                           <  1:133107/250‑1 (MQ=255)                                                                                        tGAAATATGGTGATGCCGTACCCATAACACAGGGACTAGCTGATAATCCGTCCATAAGGTTACAATCGGTACAGCAGGTTTTTTCAATTTTATCCAGGAGACGGAAATGTCATACAGCGGCGAACGAGATAACTTTGCACGCCATATGGCGCTGGTGCCGATGGTCATTGAACAGACCTc                                                                                                                                                                                                                  <  1:18577/180‑1 (MQ=255)                                                                                        tGAAATATGGTGATGCCGTACCCATAACACAGGGACTAGCTGATAATCCGTCCATAAGGTTACAATCGGTACAGCAGGTTTTTTCAATTTTATCCAGGAGACGGAAATGTCATACAGCGGCGAACGAGATAACTTTGCACGCCATATGGCGCTGGTGCCGATGGTCATTGAACAGACCTc                                                                                                                                                                                                                  >  2:18577/1‑180 (MQ=255)                                                                                         gAAATATGGTGATGCCGTTCCCATAACCCAGGGACTAGCTGATAATCCGTCCATAAGGTTACAAGCGGTACAGCAGGTTTTTTCAATTTTATCCAGGAGACGGAAATGTCATACAGCGTCGAACGAGATAACTTTGCACGCGATATGGCGCTGGTGCCGATGGTCATTGAACAGTCCTCACGCGGTGAGCGCTCTTTTGATATCTAGTCTCGTCTACTTAAGGAACGCTGT‑AGTTTTCTGACTGGCCAgg                                                                                                                                          <  2:324778/250‑1 (MQ=255)                                                                                          aaaTATGGTGATGCCGTACCCATAACACAGGGACTAGCTGATAATCCGTCCATAAGGTTACAATCGGTACAGCAGGTTTTTTCAATTTTATCCAGGAGACGGAAATGTCATACAGCGGCGAACGAGATAACTTTGCACGCCATATGGCGCTGGTGCCGATGGTCATTGAACAGACCTCACGCGGTGAGCGCTCTTTTGATATCTATtctc                                                                                                                                                                                  >  1:195277/1‑210 (MQ=255)                                                                                          aaaTATGGTGATGCCGTACCCATAACACAGGGACTAGCTGATAATCCGTCCATAAGGTTACAATCGGTACAGCAGGTTTTTTCAATTTTATCCAGGAGACGGAAATGTCATACAGCGGCGAACGAGATAACTTTGCACGCCATATGGCGCTGGTGCCGATGGTCATTGAACAGACCTCACGCGGTGAGCGCTCTTTTGATATCTATtctc                                                                                                                                                                                  <  2:195277/210‑1 (MQ=255)                                                                                                   gATGCCGTACCCATAACACAGGGACTAGCTGATAATCCGTCCATAAGGTTACAATCGGTACAGCAGGTTTTTTCAATTTTATCCAGGAGACGGAAATGTCATACAGCGGCGAACGAGATAACTTTGCACGCCATATGGCGCTGGTGCCGATGGTCATTGAACAGACCTCACGCGGTGAGCGCTCTTTTGATATCTATTCTCGTCTACTTAAGGAACGC‑GTCATTTTTCTGACTGGCCAGGTTGAAGACca                                                                                                                                <  1:278913/250‑1 (MQ=255)                                                                                                    aTGCCGTACCCATAACACAGGGACTAGCTGATAATCCGTCCATAAGGTTACAATCGGTACAGCAGGTTTTTTCAATTTTATCCAGGAGACGGAAATGTCATACAGCGGCGAACGAGATAACTTTGCACGCCATATGGCGCTGGTACCGATGGTCATTGAACAGACCTCACGCGGTGAGCGCTCTTTTGATATCTATTCTCGTCTACTTAAGGAACGC‑GTCATTTTTCTGACTGGCCAGGTTGAAGACcac                                                                                                                               >  1:287841/1‑250 (MQ=255)                                                                                                                 aaCACAGGGACTAGCTGATAATCCGTCCATAAGGTTACAATCGGTACAGCAGGTTTTTTCAATTTTAGCCAGGAGACGGAAATGTCATACAGCGGCGAACGAGATAACTTTGCACGCCATATGGCGCTGGTGCCGATGGTCATTGAACAGACCTCACGCGGTGAGCGCTCTTTTGATATCTATTCTCGTCTACTTAAGGAACGC‑GTCATTTTTCTGACTGGCCAGGTTGAAGACCACATGGCTAACCTGa                                                                                                                  <  2:311443/250‑1 (MQ=255)                                                                                                                    acaGGGACTAGCTGATAATCCGTCCATAAGGTTACAATCGGTACAGCAGGTTTTTTCAATTTTATCCAGGAGACGGAAATGTCATACAGCGGCGAACGAGATAACTTTGCACGCCATATGGCGCTGGTGCCGATGGTCATTGAACAGACCTCACGCGGTGAGCGCTCTTTTGATATCTATTCTCGTCTACTTAAGGAACGC‑GTCATTTTTCTGACTGGCCAGGTTGAAGACCACATGGCTAACCTGATtgt                                                                                                              <  1:143854/251‑1 (MQ=255)                                                                                                                                                     aCAATCGGTACAGCAGGTTTTTTCAATTTTATCCAGGAGACGGAAATGTCATACAGCGGCGAACGAGATAACTTTGCACGCCATATGGCGCTGGTGCCGATGGTCATTGAACAGACCTCACGCGGTGAGCGCTCTTTTGATATCTATTCTCGTCTACTTAAGGAACGC‑GTCATTTTTCTGACTGGCCAGGTTGAAGACCACATGGCTAACCTGATTGTGGCGCAGATGCTGTTCCTGGAAGCGGAAAAcc                                                                              <  1:295778/250‑1 (MQ=255)                                                                                                                                                     aCAATCGGTACAGCAGGTTTTTTCAATTTTATCCAGGAGACGGAAATGTCATACAGCGGCGAACGAGATAACTTTGCACGCCATATGGCGCTGGTGCCGATGGTCATTGAACAGACCTCACGCGGTGAGCGCTCTTTTGATATCTATTCTCGTCTACTTAAGGAACGC‑GTCATTTTTCTGACTGGCCAGGTTGAAGACCACATGGCTAACCTGATTGTGGCGCAGATGCTGTTCCTGGAAGCGGAAAAcc                                                                              <  1:281951/250‑1 (MQ=255)                                                                                                                                                     aCAATCGGTACAGCAGGTTTTTTCAATTTTATCCAGGAGACGGAAATGTCATACAGCGGCGAACGAGAGAACTTTGCACGCCATATGGCGCTGGTGCCGATGGTCATTGAACAGACCTCACGCGGTGAGCGCTCTTTTGATATCTATTCTCGTCTACTTAAGGAACGC‑GTCAGTTGTCTGGCGGGCCAGGTGGAAGACCACATTGCTGACCGGATTTTGGCGCAGAGGCTGGTCCTGGAAGCGTAAAAcc                                                                              <  2:46598/250‑1 (MQ=255)                                                                                                                                                          cGGTACAGCAGGTTTTTTCAATTTTATCCAGGAGACGGAAATGTCATACAGCGGCGAACGAGATAACTTTGCACGCCATATGGCGCTGGTGCCGATGGTCATTGAACAGACCTCACGCGGTGAGCGCTCTTTTGATATCTATTCTCGTCTACTTAAGGAACGC‑GTCATTTTTCTGACTGGCCAGGTTGAAGACCACATGGCTAACCTGATTGTGGCGCAGATGCTGTTCCTGGAAGCGGAAAACCCAGaaa                                                                        <  1:282855/251‑1 (MQ=255)                                                                                                                                                                             aaTTTTATCCAGGAGACGGAAATGTCATACAGCGGCGAACGAGATAACTTTGCACGCCATATGGCGCTGGTGCCGATGGTCATTGAACAGACCTCACGCGGTGAGCGCTCTTTTGATATCTATTCTCGTCTACTTAAGGAACGC‑GTCATTTTTCTGACTGGCCAGGTTGAAGACCACATGGCTAACCTGATTGTGGCGCAGATGCTGTTCCTGGAAGCGGAAAACCCAGAAAAAGATATCTATCTGTACAt                                                     <  1:228898/251‑1 (MQ=255)                                                                                                                                                                                     ccAGGAGACGGAAATGTCATACAGCGGCGAACGAGATAACTTTGCACGCCATATGGCGCTGGTGCCGATGGTCATTGAACAGACCTCACGCGGTGAGCGCTCTTTTGATATCTATTCTCGTCTACTTAAGGAACGC‑GTCATTTTTCTGACTGGCCAGGTTGAAGACCACATGGCTAACCTGATTGTGGCGCAGATGCTGTTCCTGGAAGc                                                                                      >  2:90331/1‑210 (MQ=255)                                                                                                                                                                                     ccAGGAGACGGAAATGTCATACAGCGGCGAACGAGATAACTTTGCACGCCATATGGCGCTGGTGCCGATGGTCATTGAACAGACCTCACGCGGTGAGCGCTCTTTTGATATCTATTCTCGTCTACTTAAGGAACGC‑GTCATTTTTCTGACTGGCCAGGTTGAAGACCACATGGCTAACCTGATTGTGGCGCAGATGCTGTTCCTGGAAGc                                                                                      <  1:90331/210‑1 (MQ=255)                                                                                                                                                                                       aGGAGACGGAAATGTCATACAGCGGCGAACGAGATAACTTTGCACGCCATATGGCGCTGGTGCCGATGGTCATTGAACAGACCTCACGCGGTGAGCGCTCTTTTGATATCTATTCTCGTCTACTTAAGGAACGC‑GTCATTTTTCTGACTGGCCAGGTTGAAGACCACATGGCTAACCTGATTGTGGCGCAGATGCTGTTCCTGGAAGCGGAAAACCCAGAAAAAGATATCTATCTGTACATTAACTCCCCa                                           <  1:137828/251‑1 (MQ=255)                                                                                                                                                                                          agaCGGAAATGTCATACAGCGGCGAACGAGATAACTTTTCACGCCATATGGCGCTGGTGCCGATGGTCATTGAACAGACCTCACGCGGTGAGCGCTCTTTTGATATCTATTCTCGTCTACTTAAGGAACGC‑GTCATTTTTCTGACTGGCCAGGTTGAAGACCACATGGCTAACCTGATTGTGGCGCAGATGCTGTTCCTGGAAGCGGAAAACCCAGAAAAAGATATCTATCTGTACATTAACTCCCCAGGc                                        <  2:35345/251‑1 (MQ=255)                                                                                                                                                                                              ggAAATGTCATACAGCGGCGAACGAGATAACTTTGCACGCCATATGGCGCTGGTGCCGATGGTCATTGAACAGACCTCACGCGGTGAGCGCTCTTTTGATATCTATTCTCGTCTACTTAAGGAACGC‑GTCATTTTTCTGACTGGCCAGGTTGAAGACCACATGGCTAACCTGATTGTGGCGCAGATGCTGTTCCTGGAAGCGGAAAACCCAGAAAAAGATATCTATCTGTACATTAACTCCCCAGGCgggg                                    >  2:69557/1‑251 (MQ=255)                                                                                                                                                                                                                           aCTTTGCACGCCATATGGCGCTGGTGCCGATGGTCATTGAACAGACCTCACGCGGTGAGCGCTCTTTTGATATCTATTCTCGTCTACTTAAGGAACGC‑GTCATTTTTCTGACTGGCCAGGTTGAAGACCACATGGCTAACCTGATTGTGGCGCAGATGCTGTTCCTGGAAGCGGAAAACCCAGAAAAAGATATCTATCTGTACATTAACTCCCCAGGCGGGGTGATCACTGCCGGGATGTCTATCTATGac       >  1:130810/1‑251 (MQ=255)                                                                                                                                                                                                                                gCACGCCATATGGCGCTGGTGCCGATGGTCATTGAACAGACCTCACGCGGTGAGCGCTCTTTTGATATCTATTCTCGTCTACTTAAGGAACGC‑GTCATTTTTCTGACTGGCCAGGTTGAAGACCACATGGCTAACCTGATTGTGGCGCAGATGCTGTTCCTGGAAGCGGAAAACCCAGAAAAAGATATCTATCTGTACATTAACTCCCCAGGCGGGGTGATCACTGCCGGGATGTCTATCTATGACACCAt  <  2:218077/251‑1 (MQ=255)                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                        GTGGGCTTTTTTTTGTCATGAATTTTGCATGGAACCGTGCGAAAAGCCTCTTTCGGTGTTAGCGTAACAACAAAAGATTGTTATGCTTGAAATATGGTGATGCCGTACCCATAACACAGGGACTAGCTGATAATCCGTCCATAAGGTTACAATCGGTACAGCAGGTTTTTTCAATTTTATCCAGGAGACGGAAATGTCATACAGCGGCGAACGAGATAACTTTGCACCCCATATGGCGCTGGTGCCGATGGTCATTGAACAGACCTCACGCGGTGAGCGCTCTTTTGATATCTATTCTCGTCTACTTAAGGAACGC‑GTCATTTTTCTGACTGGCCAGGTTGAAGACCACATGGCTAACCTGATTGTGGCGCAGATGCTGTTCCTGGAAGCGGAAAACCCAGAAAAAGATATCTATCTGTACATTAACTCCCCAGGCGGGGTGATCACTGCCGGGATGTCTATCTATGACACCAT  >  NC_000913/456484‑456957

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑