Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,223,638	T→C	intergenic (‑10/‑190)	iclR ← / → metH	DNA‑binding transcriptional repressor IclR/cobalamin‑dependent methionine synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,223,638	0	T	C	100.0%	77.9 / NA	22	intergenic (‑10/‑190)	iclR/metH	DNA‑binding transcriptional repressor IclR/cobalamin‑dependent methionine synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (5/17);  total (5/17)

CAACCTGACGCACGAAACCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGG‑TTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAAT‑CATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATT  >  NC_000913/4223400‑4223875                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                               cAACCTGACGCACGAAACCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGG‑TTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATc                                                                                                                                                                                                                                    <  1:105306/251‑1 (MQ=255)             cGAAACCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGG‑TTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAAt‑catt                                                                                                                                                                                                                       <  1:280771/251‑1 (MQ=255)               aaaCCCTGCTGTTGCATCGGGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAAGTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGG‑TTTTCTGCCGCGTTTCGCGGGAATGGGTTCGACCATGACAGTCTCCTTGTGCTGTATCGTGGAAAt‑catttt                                                                                                                                                                                                                     <  2:159891/251‑1 (MQ=255)                aaCCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGG‑TTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAAt‑cattttc                                                                                                                                                                                                                    <  1:109190/251‑1 (MQ=255)                                 gTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGG‑TTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAAT‑CATTTTCATTTTTATTGTTAGCTa                                                                                                                                                                                                   <  2:308811/251‑1 (MQ=255)                                        gCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGG‑TTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAAT‑CATTTTCATTTTTATTGTTAGCTAATGCAAt                                                                                                                                                                                            <  1:45180/251‑1 (MQ=255)                                                                     ccGGCTTGTGGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAGCCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGG‑TTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAAT‑CATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATg                                                                                                                                                               <  2:91353/251‑1 (MQ=255)                                                                      cGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTAGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCCGTCCAGCCGCTGGTGCGGTGGCAACGGCGGG‑ATTTCTGCCCCGCCTCGCGGGAATGGGGGCGGCCATGACCCTCTCCTTTTTCTTTATCGCGGAAAT‑CATTTTTCTTTTTTTTGTTTGCTAATTCCATAGTTTCTGAACTGATCCGATGAGTTAATGt                                                                                                                                                              >  1:168147/1‑251 (MQ=255)                                                                               cctccAGTTCCGTGATTGCCAGACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGG‑TTTTCTGCCGTGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAAT‑CATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAAt                                                                                                                                                     <  2:277926/248‑1 (MQ=255)                                                                                         cGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGG‑TTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAAT‑CATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGc                                                                                                                                           <  2:22422/251‑1 (MQ=255)                                                                                          gTGAGTGCCACACTGCCATTGGATTCGGCAATCCTCTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGG‑TTTTCTGCCGCGTTTCGCGTGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAAT‑CATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTTATCCGATGAGTGAATGTTGAACAAAGCGCATGTTGCg                                                                                                                                          <  2:115627/251‑1 (MQ=255)                                                                                                      cTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGCCTGAAACTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGA‑GGTTCTTCCACGGTTTGCGGGGATGGGGGCGACCATTCCAAGCTCCTTTTTTTGTAGCGTGGAAAAACATTTTTTTTTTTTTTTTTTG‑TTATTCAATAGTTTCTGAACTTATACGATGTGGTATTGTTGTAAAAATCTCATTTTACGTTGTTGTCGCtt                                                                                                                              >  2:108279/1‑251 (MQ=255)                                                                                                                        aTCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGG‑TTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAAT‑CATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTa                                                                                                            <  2:327234/251‑1 (MQ=255)                                                                                                                                             ccACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGG‑TTTTCTGCCGCGTGTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAAT‑CATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAAt                                                                                       <  2:153464/251‑1 (MQ=255)                                                                                                                                                 gcgTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGG‑TTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAAT‑CATTTTCATTTTTATTGTTAGCGAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAAttttt                                                                                   <  2:101043/251‑1 (MQ=255)                                                                                                                                                       aaGACTGAACCGGTCCAGTCGCTGGTGCGGTGGCAACGGCGGG‑TTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTGTTTCTGTAGCGTGGAAAT‑CATTTTCATTTTTATTGGTAGCTAATGCAATAGTTAGTGAGCTGATCCGATGAATTAATGTTGAGCAAATGTCAGGTTGCGGTGTGGTCGCTTTTACCACAGATGCGTGTATGCCAGTATGGTTTTTTGAGTTTCTGTTaaa                                                                             <  2:168147/251‑1 (MQ=255)                                                                                                                                                               aCCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTTC‑GCCGCGTTTCGCGGGAATGGGGGCGACCCCTCCAGGCTCCCTTTTTTGTATCGTGGGAAG‑CATTTTCATTTTTTTTGTTAGCTTATGCAATAGTTAATGAACTTATCCGATGGGTTTATGTTGTAAAAAACTCATTTTGCGTGGTGGTCCCTTTTTCCACAGAAGCGTTTATGCCAGATTGGGTTGTTGTAtttttttttaaacttgggt                                                                     >  2:304729/1‑237 (MQ=255)                                                                                                                                                                   gTCCAGTCGCTGGTGCGGTGGCAACGGCGGG‑TTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAAT‑CATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAg                                                                  <  1:99972/250‑1 (MQ=255)                                                                                                                                                                                        cAACGGCGGG‑TTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAAT‑CATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGagc                                            <  1:26848/251‑1 (MQ=255)                                                                                                                                                                                                                     gggAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAAT‑CATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGt                >  1:287408/1‑251 (MQ=255)                                                                                                                                                                                                                                   ccATGACAGTCTCCTTTGTCGGTATCGTGGAAAT‑CATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGAGCCGATGAGTTAATGTTGAACAGATCTCATGTTGCGTGTTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTGTGATTAAATCTGGGTTTAGCGTGTCGGGAGCGAGTTGGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGATCGTAtt  <  1:216088/251‑1 (MQ=255)                                                                                                                                                                                                                                   ccATGACAGTCTCCTGTTTCTGTATCGTGGAAAT‑CATTTTCATTTTTATTGTTAGCTAATGCACTAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCATTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGCGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGatcgaatt  >  2:186343/1‑244 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                               CAACCTGACGCACGAAACCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGG‑TTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAAT‑CATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATT  >  NC_000913/4223400‑4223875

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑