Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,029,550	C→T	R4R (CGC→CGT)	yedA →	putative transporter YedA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,029,550	0	C	T	100.0%	45.3 / NA	14	R4R (CGC→CGT)	yedA	putative transporter YedA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/5);  total (9/5)

GGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTAACAATCAAGTGCAATTATATCTCTCGCCAGCAGAATGATTTTCACGTTTACTATGAACCACCTTTTTATTTCCACCGTGAGGGATTATGCGTTTCCGCCAGTTGTTACCGCTTTTTGGCGCGCTGTTTGCGTTGTATATCATTTGGGGCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACTCCCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGC  >  NC_000913/2029319‑2029777                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                    ggCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCAATTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTAACAATCAAGTGCAATTATATCTCTCGCCAGCAGAATGATTTTCACGTTTACTATGAACCACCTTTTTATTTCCACCGTGAGGGATTATGCGTTTCCGTCAGTTGTTACCGCTTTTTg                                                                                                                                                                                                                  <  1:183937/251‑1 (MQ=255)        acatcGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTAACAATCAAGTGCAATTATATCTCTCGCCAGCAGAATGATTTTCACGTTTACTATGAACCACCTTTTTATTTCCACCGTGAGGGATTATGCGTTTCCGTCAGTTGTTACCGCTTTTTGGCGCGCt                                                                                                                                                                                                           <  1:10571/250‑1 (MQ=255)                                          aaaGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTAACAATCAAGTGCAATTATATCTCTCGCCAGCAGAATGATTTTCACGTTTACTATGAACCACCTTTTTATTTCCACCGTGAGGGATTATGCGTTTCCGTCAGTTGTTACCGCTTTTTGGCGCGCTGTTTGCGTTGTATATCATTTGGGGCTCAACCTAt                                                                                                                                                                         <  1:184025/251‑1 (MQ=255)                                                        cATTTTTATTGGTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATTCTTGCTTCACAGGTCAACATTTTTTTAACAATCAAGTGCAATTATATCTCTCGCCAGCATAATGATTTTCACGTTTACTATGAACCACCTTTTTATTTCCACCGTTAGGGATTATGCGTTGCCGTCATTTGTTTCCGCTTTTTGGCGCGCTGTTTGCGTTGTATATCATTTGGGGCTCAACCTATTTTGTCATTCGGAt                                                                                                                                                           <  1:218091/251‑1 (MQ=255)                                                                                                                 ttCACAGGTCAACATTTTTTTAACAATCAAGTGCAATTATATCTCTCGCCAGCAGAATGATTTTCACGTTTACTATGAACCACCTTTTTATTTCCACCGTGAGGGATTATGCGTTTCCGTCAGTTGTTACCGCTTTTTGGCGCGCTGTTTGCGTTGTATATCATTTGGGGCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCgg                                                                                                  >  2:112379/1‑251 (MQ=255)                                                                                                                   cacaGGTCAACATTTTTTTAACAATCAAGTGCAATTATATCTCTCGCCAGCAGAATGATTTTCACGTTTACTATGAACCACCTTTTTATTTCCACCGTGAGGGATTATGCGTTTCCGTCAGTTGTTACCGCTTTTTGGCGCGCTGTTTGCGTTGTATATCATTTGGGGCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGt                                                                                                 <  1:321079/250‑1 (MQ=255)                                                                                                                     caGGTCAACATTTTTTTAACAATCAAGTGCCATTATATCTCTCGCCAGCAGAATGATTTTCACGTTTACTATGAACCACCTTTTTATTTCCACCGTGAGGGATTATGCGTTTCCGTCAGTTGTTACCGCTTTTTGGCGCGCTGTTTGCGTTTTATATCATTTGGGGCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGTCGTTCGATTCCTGGCAGCCGGTAtt                                                                                              >  2:38058/1‑251 (MQ=255)                                                                                                                                            cAAGTGCAATTATATCTCTCGCCAGCAGAATGATTTTCACGTTTACTATGAACCACCTTTTTATTTCCACAGTGAGGGATAATGCGTTTCCGTCATTTGTTACCGCTTTTTGGCGAGCTGTTAGCTTTGTATATCATTTGGGGCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTTGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGGCTTTTATTGCTGGCAttttggttacc                                                                       >  1:289081/1‑244 (MQ=255)                                                                                                                                                 gCAATTATATCTCTCGCCAGCAGAATGATTTTCACGTTTACTATGAACCACCTTTTTATTTCCACCGTGAGGGATTATGCGTTTCCGTCAGTTGTTACCGCTTTTTGGCGCGCTGTTTGCGTTGTATATCATTTGGGGCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGATGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGGTTTTTTTTGGTGGAATTTTTGCTACTGcgcg                                                                  >  2:305526/1‑251 (MQ=255)                                                                                                                                                     ttATATCTCTCGCCAGCAGCATGATTTTCACGTTTACTATGAACCACCTTTTTATTTCCACCGTGAGGGATTATGCGTTTCCGTCAGTTGTTACCGCTTTTTGGCGCGCTGTTTGCGTTGTATATCATTTGGGGCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGGTTCCTGGCAGCCGGTATTTTTTTGCTGGCATTTTTGCTACTGCGCGGac                                                               >  2:24368/1‑250 (MQ=255)                                                                                                                                                                                              aaCCACCTTTTTATTTCCACCGTGAGGGATTATGCGTTTCCGTCAGTTGTTACCGCTTTTTGGCGCGCTGTTTGCGTTGTATATCATTTGGGGCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACTCCCCCCGCTACGTCCGCTGCTCAATGCCGCGCTg                      >  1:222715/1‑250 (MQ=255)                                                                                                                                                                                                 caccTTTTTATTTCCACCGTGAGGGATTATGCGTTTCCGTCAGTTATTACCGCTTTTTGGCGCGCTGTTTGCGTTGTATATCATTTGGGGCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGCTTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACTCCCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTg                  >  1:150015/1‑251 (MQ=255)                                                                                                                                                                                                             tCCACCGTGAGGGATTATGCGTTTCCGTCAGTTGTTACCGCTTTTTGGCGCGCTGTTTGAGTTGTATATCATTTGGGGCTCAACCTATTTTGTAATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATTCTGGCGGGCGTTCGGGTCCTGGCATCCGGTATTTTATTGCTGGCATTTTTGCTACTTCGCGGACACAACCTACCCCAGCTACGTTCGCTGCTCAATGCCGCGCCGGTTGGCCTGTAATTgc      >  2:249312/1‑251 (MQ=255)                                                                                                                                                                                                                 ccGTGAGGGATTATGCGTTTCCGTCAGTTGTTACCGCTTTTTGGCGCGCTGTTTGCGTTGTATATCATTTGGGGCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACTCCCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTgctggc  >  1:234544/1‑251 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                    GGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTAACAATCAAGTGCAATTATATCTCTCGCCAGCAGAATGATTTTCACGTTTACTATGAACCACCTTTTTATTTCCACCGTGAGGGATTATGCGTTTCCGCCAGTTGTTACCGCTTTTTGGCGCGCTGTTTGCGTTGTATATCATTTGGGGCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACTCCCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGC  >  NC_000913/2029319‑2029777

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑