Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,032,183	C→T	W45* (TGG→TGA)	dcm ←	DNA‑cytosine methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,032,183	0	C	T	100.0%	78.0 / NA	22	W45* (TGG→TGA)	dcm	DNA‑cytosine methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/10);  total (12/10)

GTTGGCTTTATAAGTGCGTACCGCATGTTTGTTCCATTCGCTGGTAAACACGCACTGTCCGCCAATCGATTCAAAACCGCGACGGATGCCGCCAATTCCGGCGAATAGATCGATAAAGCGAAACGCATAATGCGGATGATGTGCCGGTGGTTTGGGTAATAACGTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTCCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCT  >  NC_000913/2031937‑2032429                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       gTTGGCTTTATAAGTGCGTACCGCATGTTTGTTCCATTCGCTGGTAAACACGCACTGTCCGCCAATCGATTCAAAACCGCGACGGATGCCGCCCATTCCGGCGAATAGATCGATAAAGCGAAACGCATAATGCGGATGATGTGCCGGTGGTTTGGGTAATAACGTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTTCAGt                                                                                                                                                                                                                                                    >  2:147204/1‑251 (MQ=255)                 cGTACCGCATGTTTGTTCCATTCGCTGGTAAACACGCACTGTCCGCCAATCGATTCAAAACCGCGACGGATGCCGCCAATTCCGGCGAATAGATCGATAAAGCGAAACGCATAATGCGGATGATGTGCCGGTGGTTTGGGTAATAACGTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTTCAGTGATTCTCACCTACAcc                                                                                                                                                                                                                                    <  2:274709/251‑1 (MQ=255)                  gTACCGCATGTTTGTTCCATTCGCTGGTAAACACGCACTGTCCGCCAATCGATTCAAAACCGCGACGGATGCCGCCAATTCCGGCGAATAGATCGATAAAGCGAAACGCATAATGCGGATGATGTGCCGGTGGTTTGGGTAATAACGTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTTCAGTGATTCTCACCTACACCa                                                                                                                                                                                                                                   <  2:109884/251‑1 (MQ=255)                                                                                           gccAATTCCGGCGAATAGATCGATAAAGCGCAACGCATAATGCGGATGATGTGCCGGTGGTTTGGGTAATAACGTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTCAAATTGCCGCGCTTCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTcc                                                                                                                                                          >  1:100075/1‑251 (MQ=255)                                                                                                 tCCGGCGAATAGATCGATAAAGCGAAACGCATAATGCGGATGAGGTGCCGGTGGTTTGGGTAATAACGTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTTCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTg                                                                                                                                                     <  1:41635/250‑1 (MQ=255)                                                                                                       gAATAGATCGATAAAGCGAAACGCATAATGCGGATGATGTGCCGGTGGTTTGGGTAATAACGTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTTCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATg                                                                                                                                              >  1:71990/1‑251 (MQ=255)                                                                                                                               aTAATGCGGATGATGTGCCGGTGGTTTGGGTAATAACGTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTTCAGTGATTCTCACCTACACCATTAAGCTGAGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCt                                                                                                                      >  2:137582/1‑251 (MQ=255)                                                                                                                                 aaTGCGGATGATGTGCCGGTGGTTTGGGTAATAACGTGTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTTCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGc                                                                                                                    <  1:314011/251‑1 (MQ=255)                                                                                                                                                  ggtggtTTGGGTAATAACGTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTTCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATGTTCCTGCATAGATTTCACCGGCCa                                                                                                   <  2:115729/251‑1 (MQ=255)                                                                                                                                                    tggtTTGGGTAATAACGTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTTCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATc                                                                                                 >  1:84898/1‑251 (MQ=255)                                                                                                                                                               ataaCCTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCTAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTTCAGTGATTCTCACCTACACCATTAAGCTGCGCCCCCAACGTGTTAACATCATACATGTGCAGCAGTTTCACCAGCATTGCTTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGctg                                                                                      <  2:248840/251‑3 (MQ=255)                                                                                                                                                                  aCGTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTTCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGAc                                                                                    >  2:207750/1‑250 (MQ=255)                                                                                                                                                                    gTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTTCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCACCGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAgg                                                                                 >  2:305376/1‑251 (MQ=255)                                                                                                                                                                        gaaGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTTCAGTGATTCTCACCTACACCATTAATCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTTCGGCATTCCCGGTGCTGTATGAATCGTTTACTGATAGATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTa                                                                              <  2:230295/248‑1 (MQ=255)                                                                                                                                                                                 cGAACTCTTTCTCACTTAAACGGTGCCATGCCTAGGCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTTCAGTGATTCTCACCTACACCATTAAGCTGCGCACCCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCAGTTACTGATATATTTTCCTGAATAGATTTCACCGGCCATCTTAGATGGCCGACAGGTTAACATAATTAc                                                                    >  2:288101/1‑251 (MQ=255)                                                                                                                                                                                                        tGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTTCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTAt                                                                                                                                               >  1:312657/1‑153 (MQ=255)                                                                                                                                                                                                        tGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTTCAGTGATTCTCACCTACACCATGAAGCTGCTCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGGATTGCCTGTGCGGCATTCCCGGTGCTGTAt                                                                                                                                               <  2:312657/153‑1 (MQ=255)                                                                                                                                                                                                              gCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTTCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCCGCGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCCcagccagc                                       >  2:149131/1‑251 (MQ=255)                                                                                                                                                                                                                cGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTTCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCgg                                     >  2:176172/1‑251 (MQ=255)                                                                                                                                                                                                                               cgcACGTTTTAAAATTGCCGCGCTTCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCt                       >  2:118558/1‑250 (MQ=255)                                                                                                                                                                                                                                gtctgTTTTAAAATCGCCTCGCTTCAGTGATTCGCACCTACACCATTAAGCTGCGCCTCCAACGTCTTAACATCATCAATGTGTATCAGTCTCTCCAGTATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATACTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGACGTCAGGTTAACATAATTACAGCCATTGAGGGCCACGCGCTCAGCCCGCGGATGaatactctgctat                     <  2:544/247‑12 (MQ=255)                                                                                                                                                                                                                                                   cgcTTCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCt  <  1:122878/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       GTTGGCTTTATAAGTGCGTACCGCATGTTTGTTCCATTCGCTGGTAAACACGCACTGTCCGCCAATCGATTCAAAACCGCGACGGATGCCGCCAATTCCGGCGAATAGATCGATAAAGCGAAACGCATAATGCGGATGATGTGCCGGTGGTTTGGGTAATAACGTTTGCAGATGGGCGAACTCTTTCTCACTTAAACGGTGCCATGCCGAGTCATTCGCCAGCGCACGTTTTAAAATTGCCGCGCTCCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCT  >  NC_000913/2031937‑2032429

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑