Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	410,666	C→T	P175S (CCT→TCT)	mak →	fructokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	410,666	0	C	T	97.7%	143.2 / ‑7.4	44	P175S (CCT→TCT)	mak	fructokinase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (16/27);  total (17/27)
Fisher's exact test for biased strand distribution p-value = 3.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

GCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCAGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAA‑TGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCCGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAGCGGACATGCGCTGAAAGGCAGTGAAATTATCCGCCTGGTTGAAGAAAGCGATCCGGTAGCGGAACTGGCATTGCGTCGCTACGAGCTGCGGCTGGCAAAATCGCTGGCACATGTCGTGAATATTCTCGATCCGGATGTGATTGTCCTGGGGGGC  >  NC_000913/410421‑410899                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                          gcgcGAGGTGGCAGCGGGATGTGCGGCTGGAAAATGTCGCTAACTGTCTGGCGGTTTCAGAAGCAGTAGATGGCGCGGCATCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAA‑TGGGCGGGCGCATAGCGGCGGCAATGGCACGGCAGGTTAGTTTGGACACAATCCGCTACCGTGGATGGACGAAGACGACCTGCGTTATCGCGAGGAAGTCTCTTGt                                                                                                                                                                                                                                      <  2:236264/251‑1 (MQ=255)      aGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCAGAAGCAGGAGAAGGCGCGGCAGCGGGAGCGAAGACGGCATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGGGGCATTCAA‑TGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGGGAGGGGACACAATTCGATACCGTGGATAGACGGAGACGAACTGCGGTATCGCGCGGGAGTCCATTGTTATTg                                                                                                                                                                                                                                 >  1:238518/1‑251 (MQ=255)       ggTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCAGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAA‑TGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAAGCCGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCTCTTGTTATTGc                                                                                                                                                                                                                                <  1:293191/251‑1 (MQ=255)             aGCGGGAAGTGCGGCTGGCAAATGACGCTAACGGTCTGGCGGTTTCAGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTAGCGGCACGGGATGCGGCGCGGGCGTGGCATTCAA‑TGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCCGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCTCTTGTTATTGCGGTaaa                                                                                                                                                                                                                          <  2:125794/251‑1 (MQ=255)              gCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCAGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAA‑TGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCCGCTACCGTGGATGGACGAAGACGAACTGTGTTATCGCGTGGAAGTCTCTTGATATTGCGGTaaa                                                                                                                                                                                                                          >  1:175421/1‑250 (MQ=255)                  gAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCAGAAGCAGTAGATGGCGCGGCAGCGTGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAA‑TGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCCGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCTCTTGTTATTGCGGTAAACAAgg                                                                                                                                                                                                                     <  1:6079/251‑1 (MQ=255)                         ggCTGGCAAATGACGCTAACTGTCTGGCGGTTTCAGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAA‑TGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCCGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCTCTTGTTATTGCGGTAAACAAGGTTGTAtt                                                                                                                                                                                                              >  1:38010/1‑251 (MQ=255)                          gCTGGCAAATGACGCTAACTGTCTGGCGGTTTCAGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAA‑TGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCCGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCTCTTGTTATTGCGGTAAACAAGGTTGTATTg                                                                                                                                                                                                             <  1:32533/251‑1 (MQ=255)                               cAAAGGACGCTAACTGTCTGGCGGTTTCAGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAA‑TGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCCGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCTCTTGTTATTGCGGTAAACAAGGTTGTATTGAAAcc                                                                                                                                                                                                        <  2:118289/251‑1 (MQ=255)                                aaaTGACGCTAACTGTCTGGCGGTTTCAGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAA‑TGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCCGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCTCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCt                                                                                                                                                                                                       <  1:310544/251‑1 (MQ=255)                                  aTGACGCTATCTGTCTTGCGGTGTCAGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTAGCGGCACGGGATGCGGCGCGGGCGTGGCATTCAA‑TGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCCGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCTCTTGTTATTGCGGTAAACAAGTTTGTATTGAAACCttt                                                                                                                                                                                                     <  2:11662/251‑1 (MQ=255)                                    gACGCTAACTGGCTGGCGGTTTCAGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAA‑TGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCCGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCTCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTAt                                                                                                                                                                                                   <  2:184539/251‑1 (MQ=255)                                        cgaaCTGTCTGGCGGTTTCAGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAA‑TGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCCGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCTCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCg                                                                                                                                                                                               <  2:16103/249‑1 (MQ=255)                                          aaCTGTCTGGCGGTTTCAGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAA‑TGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCCGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCTCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCggg                                                                                                                                                                                             <  2:183260/251‑1 (MQ=255)                                                             aaGCAGGAGATGGCGCGGCAGCGGGAGCGCTGAGGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAAGTGGG‑GTGCGCATATCGGCGGCAATGGCACGGGAGGTGAGTGGGGACACAATCCGCTACCTTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCTCTTGTTATTGCGTTAATCAGGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGAt                                                                                                                                                                          <  1:190450/251‑1 (MQ=255)                                                                    aGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAA‑TGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCCGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCTCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTAtcgtc                                                                                                                                                                   <  1:308682/251‑1 (MQ=255)                                                                            cgGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAA‑TGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCCGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCTCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAGc                                                                                                                                                           >  1:118970/1‑251 (MQ=255)                                                                                 gCGGGAGCGCAGACGGTATTTGCCGTGATTAGCGGCACGGGATGCGGCGCGGGCGTGGCATTCAA‑TGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCCGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCTCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTGCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAGCGGACa                                                                                                                                                      <  2:38010/251‑1 (MQ=255)                                                                                   gggAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAA‑TGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGGGAGTGGGGACACAATCCGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCTCTTGTTATTGCGGTAAAAAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAGCGGACATg                                                                                                                                                    >  1:290174/1‑251 (MQ=255)                                                                                     gAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAA‑TGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCCGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCTCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAGCGGACATgcg                                                                                                                                                  <  2:242566/251‑1 (MQ=255)                                                                                             aCGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAA‑TGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCCGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCTCGTGTGATTGCGGTAAACAAGGTTGTATGGAAACCTTTATTTCGGTCACGGGATTCGCGATGGATTATCGTCGTTTGAGCGGACATGCGCTGAAAgg                                                                                                                                          <  1:189760/251‑1 (MQ=255)                                                                                                  aTTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAA‑TGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGGGAGTGGGGACACAATCCGCTACCGTGGAAGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCTCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAGCGGACATGCGCTGAAAGGCAggg                                                                                                                                     >  2:84507/1‑249 (MQ=255)                                                                                                      gCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAA‑TGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCCGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCTCTTGTTATTGCGGTAAACAAGGTTGTATTGAACCCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAGCGGACATGCGCTGAAAGGCAGTGAAAt                                                                                                                                 <  1:13747/251‑1 (MQ=255)                                                                                                         gTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAA‑TGGGACGGCGCATATCGGCGGACATGGCACGGAGGGTGAGTGGGGACACAATCCACTACCGAGGATGGACGAAGAAGAACTGCGTAATAGAGAGGGAGGCTCTTGTTAGTGCGGTAAACAAGGTTGTATTAGAACATTTATTTCGGGCACGGGATGCGGGGTGGTATATCGGAGTTTGAGCGGACAGGCGCTGACAgggcgaggagatat                                                                                                                              >  2:141072/1‑239 (MQ=255)                                                                                                                   gCACGGGATGCGGCGCGGGCGTGGCATTCAA‑TGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCCGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCTCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAGCGGACATGCGCTGAAAGGCAGTGAAATTATCCGcc                                                                                                                         >  1:46447/1‑246 (MQ=255)                                                                                                                   gCACGGGATGCGGCGCGGGCGTGGCATTCAA‑TGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCCGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCTCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAGCGGACATGCGCTGAAAGGCAGTGAAATTATCCGcc                                                                                                                         <  2:46447/246‑1 (MQ=255)                                                                                                                   gCACGGGATGCGGCGCGGGCGTGGCATTCAA‑TGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCCGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCTCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTACCGTCGGTTGAGCGGACATGCGCTGAAAGGCAGTGAAATTATCCGCCTGGtt                                                                                                                    <  1:135001/251‑1 (MQ=255)                                                                                                                              ggcgCGGGCGTGGCATTCAC‑TGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCCGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCTCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTTGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAGCGGACATGCGCTGAAAGGCAGTGAAATTATCCGCCTGGTTGAAGAAAGCg                                                                                                          >  1:124232/1‑250 (MQ=255)                                                                                                                                     gCGTGGGAGTCAA‑TGGGCGGGCGCATATCGGCGGCAATGGCACAGCAGGTGAGTGGGGACACAATCCGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCTCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAGCGGACATGCGCTGAAAGGCAGTGAAATTATCCGCCTGGTTGAAGAAAGCGATCCGGTa                                                                                                  <  1:9075/251‑1 (MQ=255)                                                                                                                                                              atatCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCCGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCTCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAGCGGACATGCGCTGAAAGGCAGTGAAATTATCCGCCTGGTTGAAGAAAGCGATCCGGTAGCGGAACTGGCATTGCGTCGCTAc                                                                          <  1:296010/251‑1 (MQ=255)                                                                                                                                                                         aaTGGCACGGCAGGTGAGTGGGGACACAATCCGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCTCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAGCGGACATGCGCTGAAAGGCAGTGAAATTATCCGCCTGGTTGAAGAAAGCGATCCGGTAGCGGAACTGGCATTGCGTCGCTACGAGCTGCGGCt                                                               <  2:182021/251‑1 (MQ=255)                                                                                                                                                                          aTGGCACGGCAGGTGAGTGGGGACACAATCCGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCTCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAGCGGACATGCGCTGAAAGGCAGTGAAATTATCCGCCTGGTTGAAGAAAGCGATCCGGTAGCGGAACTGGCATTGCGTCGCTACGAGCTGCGGCTg                                                              <  2:118970/251‑1 (MQ=255)                                                                                                                                                                            ggCACGGCAGGTGAGTGGGGACACAATCCGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCTCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAGCGGACATGCGCTGAAAGGCAGTGAAATTATCCGCCTGGTTGAAGAAAGCGATCCGGTAGCGGAACTGGCATTGCGTCGCTACGAGCTGCGGCTGGc                                                            <  1:1588/251‑1 (MQ=255)                                                                                                                                                                                         aGTGGGGACACAATCCGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCTCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAGCGGACATGCGCTGAAAGGCAGTGAAATTATCCGCCTGGTTGAAGAAAGCGATCCGGTAGCGGAACTGGCATTGCGTCGCTACGAGCTGCGGCTGGCAAAATCGCTGGc                                                >  2:286947/1‑250 (MQ=255)                                                                                                                                                                                               gACACAATCCGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCTCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAGCGGACATGCGCTGAAAGGCAGTGAAATTATCCGCCTGGTTGAAGAAAGCGATCCGGTAGCGGAACTGGCATTGCGTCGCTACGAGCTGCGGCTGGCAAAATCGCTGGCACATGTc                                         >  1:241724/1‑251 (MQ=255)                                                                                                                                                                                                   caATCCGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCTCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAGCGGACATGCGCTGAAAGGCAGTGAAATTATCCGCCTGGTTGAAGAAAGCGATCCGGTAGCGGAACTGGCATTGCGTCGCTACGAGCTGCGGCTGGCAAAATCGCTGGCACATGTCGTGa                                     <  2:241724/251‑1 (MQ=255)                                                                                                                                                                                                         gCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCTCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAGCGGACATGCGCTGAAAGGCAGTGAAATTATCCGCCTGGTTGAAGAAAGCGATCCGGTAGCGGAACTGGCATTGCGTCGCTACGAGCTGCGGCTGGCAAAATCGCTGGCACATGTCGTGAATATtc                               <  1:57526/251‑1 (MQ=255)                                                                                                                                                                                                             ccggggatggaCGAAGACGAACTGCGTTATAGCGAGGAAGTCTCTTGGTATTGCGGTAAACAAGGTTGTATTGAATCCTTTATTGCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAGCGGACATGCGCTGAAAGGCAGTGAAATTATCCGCCTGGTTGAAGAAAGCGTTCCGGTAGCGGAACTGGCATTGCGTCGCTACGAGCTGCGGCTGGCAAAATCACTGGCACAAGTCGTGAATATTCTCGa                           <  2:135357/247‑1 (MQ=255)                                                                                                                                                                                                                      gacgaagacgaaATGCGTTATCGCGAGGAAGTCTCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAGCGGACATGCGCTGAAAGGCAGTGAAATTATCCGCCTGGTTGAAGAAAGCGATCCGGTAGCGGAACTGGCATTGCGTCGCTACGAGCTGCGGCTGGCAAAATCGCTGGCACATGTCGTGAATATTCTCGATCCGGAtgt                  >  2:277403/1‑251 (MQ=255)                                                                                                                                                                                                                           agacgaaCTGCGTTATCGCGAGGAAGTCTCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAGCGGACATGCGCTGAAAGGCAGTGAAATTATCCGCCTGGTTGAAGAAAGCGATCCGGTAGCGGAACTGGCATTGCGTCGCTACGAGCTGCGGCTGGCAAAATCGCTGGCACATGTCGTGAATATTCTCGCTCCGGATGTGTTTg             >  2:186495/1‑251 (MQ=255)                                                                                                                                                                                                                            gacgaaCTGCGTTATCGCGAGGAAGTCTCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAGCGGACATGCGCTGAAAGGCAGTGAAATTATCCGCCTGGTTGAAGAAAGCGATCCGGTAGCGGAACTGGCATTGCGTCGCTACGAGCTGCGGCTGGCAAAATCGCTGGCACATGTCGTGAATATTCTCGATCCGGATGTGATTGt            >  1:297966/1‑251 (MQ=255)                                                                                                                                                                                                                                     cGTTATCGCGAGGAAGTCTCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAGCGGACATGCGCTGAAAGGCAGTGAAATTATCCGCCTGGTTGAAGAAAGCGATCCGGTAGCGGAACTGGCATTGCGTCGCTACGAGCTGCGGCTGGCAAAATCGCTGGCACATGTCGTGAATATTCTCGATCCGGATGTGATTGTCCTgggggg   >  2:222132/1‑251 (MQ=255)                                                                                                                                                                                                                                     cGTTATCGCGAGGAAGTCTCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAGCGGACATGCGCTGAAAGGCAGTGAAATTATCCGCCTGGTTGAAGAAAGCGATCCGGTAGCGGAACTGGCATTGCGTCGCTACGAGCTGCGGCTGGCAAAATCGCTGGCACATGTCGTGAATATTCTCGATCCGGATGTGATTGTCCTgggggg   >  1:140533/1‑251 (MQ=255)                                                                                                                                                                                                                                      gTTATCGCGAGGAAGTCTCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAGCGGACATGCGCTGAAAGGCAGTGAAATTATCCGCCTGGTTGAAGAAAGCGATCCGGTAGCGGAACTGGCATTGCGTCGCTACGAGCTGCGGCTGGCAAAATCGCTGGCACATGTCGTGAATATTCTCGATCCGGATGTGATTGTCCTGGGGGGc  >  1:264027/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                          GCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCAGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAA‑TGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCCGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAGCGGACATGCGCTGAAAGGCAGTGAAATTATCCGCCTGGTTGAAGAAAGCGATCCGGTAGCGGAACTGGCATTGCGTCGCTACGAGCTGCGGCTGGCAAAATCGCTGGCACATGTCGTGAATATTCTCGATCCGGATGTGATTGTCCTGGGGGGC  >  NC_000913/410421‑410899

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑