Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,730,560	C→T	W69* (TGG→TAG)	xylA ←	xylose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,730,560	0	C	T	97.2%	114.5 / ‑6.3	36	W69* (TGG→TAG)	xylA	xylose isomerase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (12/23);  minor base A (0/1);  total (12/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGAGGGTTTGTAAAGCAGTTGGCCGTTCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCCACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCCGCGGGTGATGGAT  >  NC_000913/3730320‑3730804                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                     cGAGGGTTTGTAAAGCAGTTGGCCGTTCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGAtt                                                                                                                                                                                                                                            <  2:185083/251‑1 (MQ=255)        ttGTAAAGCAGTTGGCCGTTCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACGCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGc                                                                                                                                                                                                                                      <  1:302726/250‑1 (MQ=255)                           tCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCATGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATcc                                                                                                                                                                                                                   <  2:200690/250‑1 (MQ=255)                                     agcTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCACTAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGACAGTGCCTCACCAGGCTGCTGCTACGGACGATTAGACGCCCCCACCCCAAACAAATCCGCCCCGTTcc                                                                                                                                                                                                         >  1:124136/1‑250 (MQ=255)                                          cACGCCGCTCTCTGCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGTACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGa                                                                                                                                                                                                   <  2:115740/251‑1 (MQ=255)                                               cgcTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACAGCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGGGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGgtg                                                                                                                                                                                              <  1:127186/251‑1 (MQ=255)                                                                       ggACAACAACCCTCTGCGAAAACTTATCGACGTACTCTTCTCACGACCCCCCCTCAGCAGAAACATCCAAATCGTGGACGCCCTAAAATCGCACCTGTACCTTGTGGAAAAACTCAAATGCGCCATCTGCTTTACGCTTCGCCAACGCCAGTGCCCCACCAGGCTGCTGCTACGGACGTTTACCCGCACCCACACCAACCATATCCCCCCCGTCCCAGCAGAAGGTGTGCCCGTCGCAggcggccaacacc                                                                                                                                                                      >  2:140592/1‑244 (MQ=255)                                                                                ccATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGCAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGctct                                                                                                                                                             >  2:60998/1‑251 (MQ=255)                                                                                    ttGCGCAAAATTATGGATGTACTCTTTTAACGACGCGCCCGCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCGCCCACTCCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCATTAGCAGGCGGCAAAACGCAAGTGCTCTTcc                                                                                                                                                          <  1:135428/250‑1 (MQ=255)                                                                                     tGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCa                                                                                                                                                         <  1:184243/250‑1 (MQ=255)                                                                                      gcgcAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAAAGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGGGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACGAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCAt                                                                                                                                                        <  2:152200/250‑1 (MQ=255)                                                                                      gcgcAAAATTATTGATGTACTCTGTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATTGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCAt                                                                                                                                                        <  2:244420/250‑1 (MQ=255)                                                                                         cAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAACCGCAAGTGCTCTTCCATACGc                                                                                                                                                    >  1:305697/1‑251 (MQ=255)                                                                                            aaTTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTa                                                                                                                                                 <  2:214036/251‑1 (MQ=255)                                                                                               tattGATGGTCTCTTTTAACGACGCTCCCTCAGTGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACAGCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCACTTCCATACGCTTAcc                                                                                                                                               <  2:103238/250‑1 (MQ=255)                                                                                                  tGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCaa                                                                                                                                            <  2:61081/250‑1 (MQ=255)                                                                                                            ttttAACGACGCGCCCTCAGGGGAAACATACACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCAGTTACAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCCACACCAGTtcgt                                                                                                                                 >  1:271900/1‑251 (MQ=255)                                                                                                             tttAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTtcg                                                                                                                                  <  2:239337/249‑1 (MQ=255)                                                                                                               tAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTtcgtcg                                                                                                                               <  2:164465/250‑1 (MQ=255)                                                                                                                        gcCCTCAGGGGACACATCCACATCGTGGAAGCACTAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAAc                                                                                                                                                                        >  1:230439/1‑200 (MQ=255)                                                                                                                        gcCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGt                                                                                                                     <  1:284452/251‑1 (MQ=255)                                                                                                                        gcCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTGTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACTCCAAACATATCCGCCCCGTTCCAGCAGAAGGTGGGCCAGTAGCAGGCGGTAAAAc                                                                                                                                                                        <  2:230439/200‑1 (MQ=255)                                                                                                                                         ccACATCTTGGAAGCAATTAAATTGCACATGTAACTTGTGGAAAAACACAAATGCGACATCTGCTTTACGCTTCGTCAACGCCAGTGCCTCGCCAGGCTGCTGCTACTGCCGATTAAACTCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTTTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGGTCGTCGGGATTGTATTGACGGAATGCTAACggg                                                                                                    <  2:301003/251‑1 (MQ=255)                                                                                                                                               cGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGa                                                                                               <  1:99878/250‑1 (MQ=255)                                                                                                                                                           aaaaTGGCACCTGTAACTGGGTGAAAAACCCAAATGCGACATCTGCGTTACGCTTCGCCAAAGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTGTGAGGATCTTTAGCCt                                                                                  <  2:305697/251‑1 (MQ=255)                                                                                                                                                                  cacaTGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATaac                                                                           >  1:8847/1‑251 (MQ=255)                                                                                                                                                                   acaTGTAACTTGTGGAAAAAGTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGACGGCATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATaacg                                                                          >  2:125305/1‑251 (MQ=255)                                                                                                                                                                    caTGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCAGCCCCGTTCCAGCAGAAGGTGTGACAGTAGCAGGCGGCAAAACGCAAGAGCTCTTCCATACGCTTACCCATCACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGTGGATTTTGTGCCTTCATaacg                                                                          >  2:117571/1‑250 (MQ=255)                                                                                                                                                                             tgtgGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCAGACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATc                                                                 <  2:147045/250‑1 (MQ=255)                                                                                                                                                                                 gAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCt                                                            >  1:114580/1‑251 (MQ=255)                                                                                                                                                                                         cAAATGGGACGTCTGCTTGACGCTTCGCCAACGCCATTGCCTCACCAGGCTGCTGCTACGGTCGATTAAACGCCCCCACCCCAAACATAGCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCTGGCGGCAAAACGCAAGTGCGCGTCCATACGCTTACCCAAAACCAGGTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTGTGAGGATTGTGAGCCTTCATAACGAACGCGTTCGAGCTGGTCaaa                                                     <  2:262949/250‑1 (MQ=255)                                                                                                                                                                                                                           cAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAAt                  >  1:101540/1‑251 (MQ=255)                                                                                                                                                                                                                               gCCTCACCCGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGTAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGATGATTTGGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGctt              <  2:58493/251‑3 (MQ=255)                                                                                                                                                                                                                               gCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACAGATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCcg              <  1:46413/251‑1 (MQ=255)                                                                                                                                                                                                                               gCCTCACCAGGCTGCTGCTACGGACGATTAAACGACCCCACACCAAACATATCCGCCCCGTTCCCGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGCCAAATTAGGCTTGCATATTGAACTCCCTCATCAGGTAATGCcg              >  2:35210/1‑251 (MQ=255)                                                                                                                                                                                                                                     cccggCTGCTGCAACGGCCGATTCAGCGCCCCCCAACCAAACATGCCCGCCCCGTTCCAGTAGAACGTGGGCGACTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCAACCGCGAGTTTGTGGTGATTGTAGTGGCCGCATGCCACAGGGTTTGACGATTTTGCGTCTTCCTAAAGCAGGCGATCGACCTGCTCAAAATTGGCTTGCATATTGAACGCCacatgcagttaatgcgccgggtg        <  2:118341/248‑23 (MQ=255)                                                                                                                                                                                                                                            gctgctACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCCGCGGGTgatggat  <  1:210840/250‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                     CGAGGGTTTGTAAAGCAGTTGGCCGTTCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCCACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCCGCGGGTGATGGAT  >  NC_000913/3730320‑3730804

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑