Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,325,000 C→T G56S (GGC→AGC)  phnC ← phosphonate/phosphate ABC transporter ATP binding subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,325,0000CT97.9% 151.5 / NA 47G56S (GGC→AGC) phnCphosphonate/phosphate ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (21/25);  minor base A (1/0);  total (22/25)
Fisher's exact test for biased strand distribution p-value = 4.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCGCCTGTAACGCGCGCTGTTTCTGCTCGCCGGTGAACCAGCTAAAACAGGTGCGCCAGAACGGCGTGCTGCCGAGCGCGCCAATCAGCACGTTCTCCAGTACGCTCAGGCGGTTCACCAGGTTGAATTGTTGGAATATGTAGCCGGTATGGGCGCGGCTTTTGCGGATATCGCGGGCCAGGCGGCCTTCGCGCTGGACTGTGCGGCCCAGCAGCTCGATATGGCTACCGACAGATTTATCGCCGGTAATCAAACCGCTTAAGTGACGTAAAAGGGTGGATTTTCCGGAACCCGACGGCCCAAGCAGAGCCACCATTTCACCGTGATGAATGTTCAGATCAACCGCATGCAGCGCCTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGATAATCGTTTGCATGATGCAGCCTCCCTAAAAAAGTGGCTCCATCGTGGCGGATTATTGTGACGATTTGGTTAATTAAAAGTGACTAACAGA  >  NC_000913/4324756‑4325243
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                   
aGCGCCTGCCACCCGCGCCGTTCCTGATCGCCGGTGCACCAGCTAAAACAGGTGCGCCCGAACGGCGTGCTGCCGAGCGCGCCAATCAGCCCGTTCTCCAGTACGCTCAGGCGGTTCACCAGGTTGAATTGTTGGAGTATGTAGCCGGTATGGGCGCGGCTTTTGCGGATCTCGCGGGCCAGGCGGCCTGCGCGCTGGAATGTGCGGCCCAGCAGCTCGCTATAGCTACCGACAGGTTTATCGCAGGTAAt                                                                                                                                                                                                                                               >  2:145258/1‑251 (MQ=255)
        tAACGCGCGCTGTTTCTGCTCGCCGGTGAACCAGCTAAAACAGGTGCGCCAGAACGGCGTGCTGCCGAGCGCGCCAATCAGCACGTTCTCCAGTACGCTCAGGCGGTTCACCAGGTTGAATTGTTGGAATATGTAGCCGGTATGGGCGCGGCTTTTGCGGATATCGCGGGCCAGGCGGCCTTCGCGCTGGACTGTGCGGCCCAGCAGCTCGATATGGCTACCGACAGATTTATCGCTGGTAATCAAACCGc                                                                                                                                                                                                                                       >  2:255338/1‑251 (MQ=255)
            gcgcgcTGTTTCTGCTCGCCGGTGAACCAGCTAAAACAGGTGCGCCAGAACGGCGTGCTGCCGAGCGCGCCAATCAGCACGTTCTCCAGTACGCTCAGGCGGTTCACCAGGTTGAATTGTTGGAATATGTAGCCGGTATGGGCGCGGCTTTTGCGGATATCGCGGGCCAGGCGGCCTTCGCGCTGGACTGTGCGGCCCAGCAGCTCGATATGGCTACCGACAGATTTATCGCTGGTAATCAAACCGCTTaa                                                                                                                                                                                                                                   <  1:255338/251‑1 (MQ=255)
               cgcTGTTTCTGCTCGCCGGTGAACCAGCTAAAACAGGTGCGCCAGAACGGCGTGCTGCCGAGCGCGCCAATCAGCACGTTCTCCAGTACGCGCAGGCGGTTCACCAGGTTGAATTGTTGGAATATGTAGCCGGTATGTGCGCGGCTTTGGCGGATATCGCGGGCCAGGCGGCCGTCGCGCTGGACTGTGCGGCCCAGCAGGTCGATATGGCTACCGACAGATTTATCGCTGGTAATCAAACCGCTTAAGTg                                                                                                                                                                                                                                <  1:212630/251‑1 (MQ=255)
                                                                  gtgtggcCGAGCGGGCAACTCTGCACGTTCCCCAGTTCTCGCAGGCGGTTCCCCAGGGGGAATTGTTGGAATATGTAGCCCGTATGGGGGCGGCTTTTGCGGATATCGCGGGCCGGGCGTCCTTCGCGCTGGACTGTGCGGCCCAGCAGCTCGATATGGCTACCGACAGATTTATCGCTGGTAATCAAACCGCTTAAGTGACGTAAAAGGGTGGATTTTCCGGATCCCGACGGCCCAAGCAGAGCCACCAt                                                                                                                                                                             <  2:174597/246‑1 (MQ=255)
                                                                  gtactgcCGAGCTCTCCAATCAGCGCGTTCTCTAGTACGATCAGGCGGTTCACCGGGTTGAATTGTTGGAATATGTAGCCGGTATGGGCGCGTCTTTTGCGGATAGCGCGGGCCAGTGGGCCTTCGCGCTGGACTGGGCGGCCCAGCAGCTCGATATGGCTACCGACAGATTTATCGCTGGTAATCAAACCGCTTAAGTGACGTAAAAGGGTGGATTTTCCGGAACCCGACGGCCCAAGCAGAGCCACCAt                                                                                                                                                                             <  1:43847/248‑1 (MQ=255)
                                                                  gTGCTGCCGAGCGCGCCAATCAGCACGTTCTCCAGTACGCTCAGGCGGTTCACCAGGTTGAATTGTTGGAATATGTACCCGGTATGGGCGCGGCTTTTGCGGATATCGCGGGCCAGGCGGCCTTCGCGCTGGACTGTCCGGCACAGCAGCTCGATATGGCTACAGCCCGATTTATCGCTGGCAATCAAACCGCTTAAGTAACGAAATAGGGTGGATTTTCCGGGAACCGACGGTCCACGCAGAGACACCAt                                                                                                                                                                             >  1:140617/1‑251 (MQ=255)
                                                                   tgctgcCGAGCGCGCCAATCAGCACGTTCTCCAGTACGCTCAGGCGGTTCACCAGGTTGAATTGTTGGAATATGTAGCCGGTATGGGCGCGGCTTTTGCGGATATCGCGGGCCAGGCGGCCTTCGCGCTGGACTGTGCGGCCCAGCAGCTCGATATGGCTACCGACAGATTTATCGCTGGTAATCAAACCGCTTAAGTGACGTAAAAGGGTGGATTTTCCGGAACCCGACGGCCCAAGCAGAGCCACCAtt                                                                                                                                                                            >  1:110659/1‑251 (MQ=255)
                                                                        ccGAGCGCGCCAATCAGCACGTTCTCCAGTACGCTCAGGCGGTTCACCAGGTTGAATTGTTGGAATATGTAGCCGGTATGGGCGCGGCTTTTGCGGATATCGCGGGCCAGGCGGCCTTCGCGCTGGACTGTGCGGCCCAGCAGCTCGATATGGCTACCGACAGATTTATCGCTGGTAATCAAACCGCTTAAGTGACGTAAAAGGGTGGATTTTCCGGAACCCGACGGCCCAAGCAGAGCCACCATTTCAcc                                                                                                                                                                       <  1:315867/251‑1 (MQ=255)
                                                                               cgcCAATCAGCACGTTCTCCAGTACGCTCAGGCGGTTCACCAGGTTGAATTGTTGGAATATGTAGCCGGTATGGGCGCGGCTTTTGCGGATATCGCGGGCCAGGCGGCCTTCGCGCTGGACTGTGCGGCCCAGCAGCTCGATATGGCTACCGACAGATTTATCGCTGGTAATCAAACCGCTTAAGTGACGTAAAAGGGTGGATTTTCCGGAACCCGACGGCCCAAGCAGAGCCACCATTTCACCGtgatg                                                                                                                                                                 >  1:193531/1‑250 (MQ=255)
                                                                                gcCAATCAGCACATTCTCCAGTACGCTCAGGCGGGTAACAAGGAAGAATTGTTGGAATATGTAGCCGGTATGGGCGCGGCTTTTGCGGATATCGCGAGCCAGGCGGCCTTCGCGCTGGACTGTGAGGCCCAGCAGCTCGATATGGCTACCGACAGATTTATCGCTGGTAATCAAACCGCTTAAGTGACGTAAAAGGGTGGATTTTGCGGCACCCGACGGCCAAAGCAGAGCCACACTTTAACCGtgagga                                                                                                                                                                >  2:232615/1‑247 (MQ=255)
                                                                                    aTCAGCACGTTCTCCAGTACGCTCAGGCGGTTCACCAGGTTGAATTGTTGGAATATGTAGCCGGTATGGGCGCGGCTTTTGCGGATATCGCGGGCCAGGCGGCCTTCGCGCTGGACTGTGCGGCCCAGCAGCTCGATATGGCTACCGACAGATTTATCGCTGGTAATCAAACCGCTTAAGTGACGTAAAAGGGTGGATTTTCCGGAACCCGACGGCCCAAGCAGAGCCACCATTTCACCGTGATGAATGtt                                                                                                                                                           >  1:65814/1‑251 (MQ=255)
                                                                                      cAGCACGTTCTCCAGTACGCTCAGGCGGTTCACCAGGTTGAATTGTTGGAATATGTAGCCGGTATGGGCGCGGCTTTTGCGGATATCGCGGGCCAGGCGGCCTTCGCGCTGGACTGTGCGGCCCAGCAGCTCGATATGGCTACCGACAGATTTATCGCTGGTAATCAAACCGCTTAAGTGACGTAAAAGGGTGGATTTTCCGGAACCCGACGGCCCAAGCAGAGCCACCATTTCACCGTGATGAATGTTc                                                                                                                                                          <  1:34103/250‑1 (MQ=255)
                                                                                      cAGCACGTTCTCCAGTACGCTCAGGCGGTTCACCAGGTTGAATTGTTGGAATAGGTAGCCGGTATGGGCGCGGCTTTTGCGGATATCGCGGGCCAGGCGGCCTTCGCGCTGGACTGTGCGGCCCAGCAGCTCGATATGGCTACCGACAGATTTATCGCTGGTAATCAAACCGCTTAAGTGACGTAAAAGGGTGGATTTTCCGGAACCCGACGGCCCAAGCAGAGCCACCATTTCACCGTGATGAATGTTCa                                                                                                                                                         <  2:65814/251‑1 (MQ=255)
                                                                                              tctcCAGTACGCTCAGGCGGTTCACCAGGTTGAATTGTTGGAATATGTAGCCGGTATGGGCGCGGCTTTTGCGGATATCGCGGGCCAGGCGGCCTTCGCGCTGGACTGTGCGGCCCAGCAGCTCGATATGGCTACCGACAGATTTATCGCTGGTAATCAAACCGCTTAAGTGACGTAAAAGGGTGGATTTTCCGGAACCCGACGGCCCAAGCAGAGCCACCATTTCACCGTGATGAATGTTCAGATCAAc                                                                                                                                                  <  1:47462/250‑1 (MQ=255)
                                                                                                 ccAGTACGCTCAGGCGTTTCACCAGGTTGGATTGGTGGAATATGTAGGCGGTATGGGCGCGGCTTTTGCGGATATCGCGGGCCAGGCGGCCTTCGCGCTGGACTGTGCGGCCCAGCAGCTCGATATGGCTACCGACAGATTTATCGCTGGTAATCAAACCGCTTAAGTGACGTAAAAGGGTGTATTTTCCGGAACCCGACGGCCCAAGCAGAGCCACCATTTCACCGTGATGAATGTTCAGATCAAcc                                                                                                                                                 <  2:168702/248‑1 (MQ=255)
                                                                                                 ccAGTACGCTCAGGCGGTTCACCAGGTTGAATTGTGGGAATATGTAGCCGGTAGGGGCGCGGCTGTTGCGGATATCGCGGGCCAGGCGGCCTTCGCGCTGGACTGTGCGGCCCAGCAGCTCGATATGGCTACCGACAGATTTATCGCTGGTAAGCAAACCGCTTAAGTGACGTAAAAGGGTGGATTTTCCGGAACCCGACGGCCCAAGCAGAGCCACCATTGCACCGTGATGAATGTTCAGATCATCCGCa                                                                                                                                              <  2:136116/251‑1 (MQ=255)
                                                                                                  catgtcgcgcAGGCGGTTCACCAGGTGGAATAGGTGGAATATGGAGTCGGTATGGGCGCGGCTTTTGCGGATATCGCGGGCCAGGCGGCCTTCACGCTGGTCTGTGCGGCCCAGCAGCTCGATATTGCTACCGACAGATTTATCGCTGGTAATCAAACCGCTTAAGTGACGTAAAAGGGTTGATTTTCAGGAACCCGCCGGCCCAAGCAGAGACACCATTCCACCGTGAGGAATGTTCAGATCAACCGCAt                                                                                                                                             <  2:50406/242‑1 (MQ=255)
                                                                                                    gTACGCTCAGGCGGTTCACCAGGTTGAATTGTTGGAATATGTAGCCGGTATGGGCGCGGCTTTTGCGGATATCGCGGGCCAGGCGGCCTTCGCGCTGGACTGTGCGGCCCAGCAGCTCGATATGGCTACCGACAGATTTATCGCTGGTAATCAAACCGCTTAAGTGACGTAAAAGGGTGGATTTTCCGGAACCCGACGGCCCAAGCAGAGCCACCATTTCACCGTGATGAATGTTCAGATCAACCGCATGc                                                                                                                                           >  1:123733/1‑251 (MQ=255)
                                                                                                       cGCTCAGGCGGTTCACCAGGTTGAATTGTTGGAATATGTAGCCGGTATGGGCGCGGCTTTTGCGGATATCGCGGGCCAGGCGGCCTTCGCGCTGGACTGTGCGGCCCAGCAGCTCGATATGGCTACCGACAGATTTATCGCTGGTAATCAAACCGCTTAAGTGACGTAAAAGGGTGGATTTTCCGGAACCCGACGGCCCAAGCAGAGCCACCATTTCACCGTGATGAATGTTCAGATCAACCGCATGCAgc                                                                                                                                        <  1:22715/251‑1 (MQ=255)
                                                                                                        gCTCAGGAGGTTCACCAGGTTGAATTGTTGGAATATGTAGCCGGTATGGGCGCGGCTTTTGCGGATATCGCGGGCCAGGCGGCCTTCGCGCTGGACTGTGCGGCCCAGCAGCTCGATATGGCTACCGACAGATTTATCGCTGGTAATCAAACCGCTTCAGTGACGTAAAAGGGAGGATTTTCCGGAACCCGACGGCCCACGCAGAGCCACCATTTAACCGTGATGAATGTTCAGATCAACCGCATGCAgcg                                                                                                                                       >  2:300702/1‑251 (MQ=255)
                                                                                                           cAGGCGGTTCGCTAGGTTTAATTGGTGTAATATGTAGCCGGTATTGGCGTGGCGTTTGCGGATATCGCGGGCCAGGCGGCCTTCGCGCTGTACTGTGCGGCCCAGCAGCTCGATATGGCTACCGACAGATTTATCGCTGGTAAGCAAACCGCGTAAGGGACGTAAAAGTGTGGATTTTCCGGAACCCGGCGGCCCAAGCAAAGGCACCATTTCACCGGGAGGAATGTTCAGATCATCCGCATGCAgcgc                                                                                                                                      <  2:15720/249‑1 (MQ=255)
                                                                                                                ggTTCACCAGGTTGAATTGTTGGACTATGTAGACGGTATGGGCGCGGCTTTTGCGGATATCGCGGGCCAGGCGGCTTTCGCGCTGGACTGTGCGGCCCAGCAGCTCGATATGGCTACCGACAGATTTATCGCTGGTAATCAAACCGCTTAAGAGCAGTAAAAGGGTGGCTTTTCCGGGACCCGCCGGCCACAGCAGAGTCACCATTTCACCGTGATGGATGTTCCGATCAACCGCCTGCCGCGCCTGATGc                                                                                                                               >  2:186749/1‑251 (MQ=255)
                                                                                                                ggTTCACCAGGTTGAATTGTTGGAATATGTAGCCGGTATGGGCGCGGCTTTTGCGGATATCGCGGGCCAGGCGGCCTTCGCGCTGGACTGTGCGGCCCAGCAGCTCGATATGGCTACCGACAGATTTATCGCTGGTAATCAAACCGCTTAAGTGACGTAAAAGGGTGGATTTTCCGGAACCCGACGGCCCAAGCAGAGCCACCATTTCACCGTGATGAATGTTCAGATCAACCGCATGCAGCGCCTGATGc                                                                                                                               >  1:157072/1‑251 (MQ=255)
                                                                                                                        aGGTTGAATTGTTGGAATATGTAGCCGGTATGGGCGCGGCTTTTGCGGATATCGCGGGCCAGGCGGCCTTCGCGCTGGACTGTGCGGCCCAGCAGCTCGATATGGCTACCGACAGATTTATCGCTGGTAATCAAACCGCTTAAGTGACGTAAAAGGGTGGATTTTCCGGAACCCGACGGCCCAAGCAGAGCCACCATTTCACCGTGATGAATGTTCAGATCAACCGCATGCCGCGCCTGATGCTGATTgc                                                                                                                        >  2:289581/1‑249 (MQ=255)
                                                                                                                         ggTTGAATTGTTGGAATTGGTAGCCGTTATGGGCGCGGCTTTTGCGGATATCGCGGGCCAGGCGGCCTTCGCGCTGGACTGTGCGGCCCAGCAGCTCGATATGGCTACCGACAGATTTATCGCTGGTAATCAAACCGCTTAAGTGACGTAAAAGGGTGGATTTTCCGGAACCCGACGGCCCAAGCAGAGCCACCATTTCACCGTGATGAATGTTCAGATCAACCGCATGCAGCGCCTGATGCTGATTGAAg                                                                                                                      <  2:113096/251‑1 (MQ=255)
                                                                                                                          ggtGAATTGTTGGAATATGTAGCCGGTATGGGCGCGGCTTTTGCGGATATCGCGGGCCAGGCGGCCTTCGCGCTGGACTGTGCGGCCCAGCAGCTCGATATGGCTACCGACAGATTTATCGCTGGTAATCAAACCGCTTAAGTGACGTAAAAGGGTGGATTTTCCGGAACCCGACGGCCCAAGCAGAGCCACCATTTCACCGTGATGAATGTTCAGATCAACCGCATGCAGCGCCTGATGCTGATTGAAgg                                                                                                                     <  1:62710/249‑1 (MQ=255)
                                                                                                                                 tgttgGAATATCTAGCCGGCATGGGCGAGGCTTTTGCGGATATCGCGGGCCAGACGGCCTTCGCGCTGGACTGTGCGGCCCCGCAGCTCGATATGGCTACCGACAGATTTATCGCTGGTAATCAAACCGCTTAAGTGACGTAAAAGGGTGGATTTTCCGGAACCCGACGGCCCACGCAGAGCCACCATTTCACCGTGATGAATGTTCCGATCAACCGCATGCAGCGCCTGATGCTGCTTGAAGGTTTTGGc                                                                                                              >  2:276137/1‑251 (MQ=255)
                                                                                                                                       aaTATGTAGCCGGTATGGGCGCTGCTTTTGCGGAGATCGCGGGCCAGGCGGCCTTCGCGCTGGACTGTGCGGCCCAGCAGCTCGATATGGCTACCGACAGATTTATCGCTGGTAATCAAACCGCTTAAGTGACGTAAAAGGGTGGATTTTCCGGAACCCGACGGCCCAAGCAGAGCCACCATTTCACCGTGATGAATGTTCAGATCAACCGCATGCAGCGCCTGATGCTGATTGAAGGTGTTGGCGAGCtt                                                                                                        <  2:39007/251‑1 (MQ=255)
                                                                                                                                            gTAGCCGGTATGGGCGCGGCTTTTGCGGATATCGCGGGCCAGGCGGCCTTCGCGCTGGACTGTGCGGCCCAGCAGCTCGATATGGCTACCGACAGATTTATCGCTGGTAATCAAACCGCTTAAGTGACGTAAAAGGGTGGATTTTCCGGAACCCGACGGCCCAAGCAGAGCCACCATTTCACCGTGATGAATGTTCAGATCAACCGCATGCAGCGCCTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCg                                                                                                    >  2:226357/1‑250 (MQ=255)
                                                                                                                                                            cgGCTTTTGCGGATATCGCGGGCCAGGCGGCCTTCGCGCTGGACTGTGCGGCCCAGCAGCTCGATATGGCTACCGACAGATTTATCGCTGGTAATCAAACCGCTTAAGTGACGTAAAAGGGTGGATTTTCCGGAAACCGACGGCCCAAGCAGAGCCACCATTTCACCGTGATGAATGTTCAGATCAACCGCATGCAGCGCCTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGATAATCGTTTg                                                                                   >  1:88636/1‑251 (MQ=255)
                                                                                                                                                              gCTTTTGCGGATATCGCGGGCCAGGCGGCCTTCGCGCTGGACTGTGCGGCCCAGCAGCTCGATATGGCTACCGACAGATTTATCGCTGGTAATCAAACCGCTTAAGTGACGTAAAAGGGTGGATTTTCCGGAACCCGACGGCCCAAGCAGAGCCACCATTTCACCGTGATGAATGTTCAGATCAACCGCATGCAGCGCCTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGATAATCGTTTGc                                                                                  >  1:325113/1‑250 (MQ=255)
                                                                                                                                                                      ggATATCGCGGGCCAGGCGGCCTTCGCGCTGGACTGTGCGGCCCAGCAGATCGATATGGCTACCGACAGATTTATCGCTGGTAATCAAACCGCTTCAGTGACGTAAAAGGGAGGATTTTCCGGAACCCGACGGCCCAAGCAGAGCCACAATTTCACCGTGATGAATGTTCAGATCAACCGCATGCAGCGCCTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGATAATCGTTTGCATGATGc                                                                           >  1:161530/1‑249 (MQ=255)
                                                                                                                                                                          atCGCGGGCCAGGCGGCCTTCGCGCTGGACTGTGCGGCCCAGCAGCTCGATATGGCTACCGACAGATTTATCGCTGGTAATCAAACCGCTTAAGTGACGTAAAAGGGTGGATTTTCCGGAACCCGACGGCCCAAGCAGAGCCACCATTTCACCGTGATGAATGTTCAGATCAACCGCATGCAGCGCCTGATGCTGATTGAAGGTTGTGGCGAGCTTCTCGACACGGATAATCGTTTGCATGATGCAGCCTc                                                                     <  1:300702/251‑1 (MQ=255)
                                                                                                                                                                          agcgcgGGCCAGGCGGCCTTCGCGCTGGACTGTGCGGCCCAGCAGCTCGATATGGCTAACGACAGATTTATCGCTGGTAATCAAACCGCTTAAGTGACGTAAAAGGGTGGATTTTCCGGAACCCGACGGCCCAAGCAGAGCCACCATTTCACCGTGATGAATGTTCAGATCAACCGCATGCAGCGCCTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGATAATCGTTTGCATGATGCAGCCt                                                                      <  2:144165/248‑1 (MQ=255)
                                                                                                                                                                              cgggcgcggcggaCTTCGCGGCGGAATGTGCGGACCAGCAGCGCGATATGGCTACCGACAGCTTTATCGCTGGTCAACAAACCGCTTAAGAGACGTACCAGGGTGGATTTTCCGGAACCCGACGGCCCAAGCAGCGCCACCACTTCACCGTGATGAATGTTAAGCTCAACCGCATGCCGCGCCTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGCCACGGTCACTCGTATGCATGATGCAGCCTCCCt                                                                  >  2:149010/8‑250 (MQ=255)
                                                                                                                                                                                  ccAGGCGGCCTTCGCGCTGGACTGTGCGGCCCAGCAGCTCGATATGGCTACCGACAGATTTATCGCTGGTAATCAAACCGCTTAAGTGACGTAAAAGGGTGGATTTTCCGGAACCCGACGGCCCAAGCAGAGCCACCATTTCACCGTGATGAATGTTCAGATCAACCGCCTGCAGCGCCTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGCCACGGCTAATCGTTTGCATGATGCAGCCTCCCTaaaa                                                              >  2:177043/1‑250 (MQ=255)
                                                                                                                                                                                  ccAGGCGGCCTTCGCGCTGGACTGTGCGGCCCAGCAGCTAGATATGGCTACCGACAGATTTATCGCTGGAAATCAAACCGCTTAAGAGACGTAAAAGGGTGGATTTTCCGGAACCCGAAGGCCCAAGCAGAGCCACCATTTCCCCGTGATGAATGTTCAGATCACCCGCATGCAGCGCCTGATGCTGATTGAAGGTTTTGGCGCGCTTCTCGACACGGATACTCGTTTGCATGATGCAGCCTCCCTaaaa                                                              >  1:46676/1‑250 (MQ=255)
                                                                                                                                                                                              cgcgCTGGACTGTGCGGCCCAGCAGCTCGATATGGCTACCGACAGATTTATCGCTGGTAATCAAACCGCTTAAGTGACGTAAAAGGGTGGATTTTCCGGAACCCGACGGCCCAAGCAGAGCCACCATTTCACCGTGATGAATGTTCAGATCAACCGCATGCAGCGCCTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGATAATCGTTTGCATGATGCAGCCTCCCTAAAAAAGTGGCTCCAt                                                  <  1:86642/250‑1 (MQ=255)
                                                                                                                                                                                                  cTGGACTGTGCGGCCCAGCAGCTCGATATGGCTACCGACAGATTTATCGCTGGTAATCAAACCGCTTAAGTGACGTAAAAGGGTGGATTTTCCGGAACCCGACGGCCCAAGCAGAGCCACCATTTCACCGTGATGAATGTTCAGATCAACCGCATGCAGCGCCTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGATAATCGTTTGCATGGTGCAGCCTCCCTAAAAAAGTGGCTCCATCGTgg                                             >  1:273264/1‑251 (MQ=255)
                                                                                                                                                                                                         gtgCGGCCCAGCAGCTCGATATGGCTACCGACAGATTTATCGCTGGTAATCAAACCGCTTAAGTGACGTAAAAGGGTGGATTTTCCGGAACCCGACGGCCCAAGCAGAGCCACCATTTCACCGTGATGAATGTTCAGATCAACCGCATGCAGCGCCTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGATAATCGTTTGCATGATGCAGCCTCCCTAAAAAAGTGGCTCCATCGTGGCGGat                                        <  1:43947/249‑1 (MQ=255)
                                                                                                                                                                                                           gCGGCCCAGCAGCTCGATATGGCTACCGACAGATTTATCGCTGGTAATCAAACCGCTTAAGTGACGTAAAAGGGTGGATTTTCCGGAACCCGACGGCCCAAGCAGAGCCACCATTTCACCGTGATGAATGTTCAGATCAACCGCATGCAGCGCCTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGATAATCGTTTGCATGATGCAGCCTCCCTAAAAAAGTGGCTCCATCGTGGCGGattat                                     <  1:226357/250‑1 (MQ=255)
                                                                                                                                                                                                             ggCCCAGCAGCTCGATATGGCTACCGACAGATTTATCGCTGGTAATCAAACCGCTTAAGTGACGTAAAAGGGTGGATTTTCCGGAACCCGACGGCCCAAGCAGAGCCACCATTTCACCGTGATGAATGTTCAGATCAACCGCATGCAGCGCCTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGATAATCGTTTGCATGATGCAGCCTCCCTAAAAAAGTGGCTCCATCGTGGCGGATTATtgt                                  <  1:173896/251‑1 (MQ=255)
                                                                                                                                                                                                                      gcTCGATATGGCGTCCGACAGTGTTATCGCTGGTAATCAAACCGCTTAAGTTACGTAAAAGGGTGGATTTTCCGGAACCCGACGGCCCAAGCAGATCCTGCATCTCACCATGATGAATTCTCAGATCAACCTCACGTAGCGCCTGATGCTGATTGAAGGTTGTGGCGAGCTTCTCGACACGGATAATCGTTTGCATGATGCTGCCTCCCTAAAAAAGTGGCTCCACCGTGGCGGATTATTGTGACGATTTg                         <  1:320030/251‑1 (MQ=255)
                                                                                                                                                                                                                           atatGGCTACCGACAGATTTATCGCTGGTAATCAAACCGCTTAAGTGACGTAAAAGGGTGGATTTTCCGGAACCCGACGGCCCAAGCAGAGCCACCATTTCACCGTGATGAATGTTCAGATCAACCGCATGCAGCGCCTGATGCTGATTGAAGGTTTTGTCGAGCTTCTCGACACGGATAATCGTTTGCATGATGCAGCCTCCCTAAAAAAGTGGCTCCATCGTGGCGGATTATTGTGACGATTTGGttaa                    <  2:78259/251‑1 (MQ=255)
                                                                                                                                                                                                                                    ccGACAGATTTATCGCTGGTAATCAAACCGCTTAAGTGACGTAAAAGGGTGGATTTTCCGGAACCCGACGGCCCAAGCAGAGCCACCATTTCACCGTGATGAATGTTCAGATCAACCGCATGCAGCGCCTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGATAATCGTTTGCATGATGCAGCCTCCCTAAAAAAGTGGCTCCATCGTGGCGGATTATTGTGACGATTTGGTTAATTAAAAGTg           >  2:252092/1‑251 (MQ=255)
                                                                                                                                                                                                                                              tATCGCTGGTAATCAAACCGCTTAAGTGACGTAAAAGGGTGGATTTTCCGGAACCCGACGGCCCAAGCAGAGCCACCATTTCACCGTGATGAATGTTCAGATCAACCGCATGCAGCGCCTGATGCTGATTGAAGGTTTTGGCGAGCTTCGCGACACGGATAATCGTGTGCATGATGCAGCCTCCCTAAAAAAGTGGCTCCATCGTGGCAGATTATTGTGACGATTTTGGTAATGAAAAGTGACTAACAGa  <  2:193531/250‑1 (MQ=255)
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                   
AGCGCCTGTAACGCGCGCTGTTTCTGCTCGCCGGTGAACCAGCTAAAACAGGTGCGCCAGAACGGCGTGCTGCCGAGCGCGCCAATCAGCACGTTCTCCAGTACGCTCAGGCGGTTCACCAGGTTGAATTGTTGGAATATGTAGCCGGTATGGGCGCGGCTTTTGCGGATATCGCGGGCCAGGCGGCCTTCGCGCTGGACTGTGCGGCCCAGCAGCTCGATATGGCTACCGACAGATTTATCGCCGGTAATCAAACCGCTTAAGTGACGTAAAAGGGTGGATTTTCCGGAACCCGACGGCCCAAGCAGAGCCACCATTTCACCGTGATGAATGTTCAGATCAACCGCATGCAGCGCCTGATGCTGATTGAAGGTTTTGGCGAGCTTCTCGACACGGATAATCGTTTGCATGATGCAGCCTCCCTAAAAAAGTGGCTCCATCGTGGCGGATTATTGTGACGATTTGGTTAATTAAAAGTGACTAACAGA  >  NC_000913/4324756‑4325243

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑