Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,281,954	C→A	N441K (AAC→AAA)	radD →	putative DNA repair helicase RadD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,281,954	0	C	A	97.3%	96.3 / NA	37	N441K (AAC→AAA)	radD	putative DNA repair helicase RadD
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (22/14);  total (23/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CACGATCTCTACGCGCCGGAAGTTGGTACACCAAAAGGCAAAAGTGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACAC‑CTTTTGGGGGAAAA‑C‑GACCGCCGACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAA‑‑AACGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCAACATGGGCACGACGAGAAAGGCGAATGGTTGAAAATCACCTATTACGATGAAGACGGCGCGGATGTGAGTGAGCGTTTCCGTCTGCAAACACCTGCCCAGCGTACCGCCTTCGAGCAGCTTT  >  NC_000913/2281712‑2282198                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                     cACGATCTCTACGCACCGGAGGTTGGTACACCAAAAGGCAAAAGTGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACAC‑CTTTTGGGGGAAAA‑C‑GACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCAGCGCGAACAATGTGACTTCCGTTTCCGTTGTAAAAATTGCCCGCAATGTAACGCGGAA‑‑AAAGATATTgc                                                                                                                                                                                                                                              <  2:315605/251‑1 (MQ=255)                              acCAACAGGCAAAAGTGACAACGTTCCGGTACAGGGTTTCTGCCCTGCCTGCGGTTTTGCCAACAC‑CTTTTGGGGGAAAA‑C‑GACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAA‑‑AAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCg                                                                                                                                                                                                                 >  2:224605/1‑251 (MQ=255)                                            gtaacaccgttcgggtaggtgTTTTCTCCCCTCCCGGGGGTTTT‑CCAACCC‑CCTTTGGGGGAAAA‑CCGACCGCGGAGGGGACATTGATTGAACCCTTTGGCCGTCGTTGTCAGGGATGGTTTGAAGATGACGACGGTCACCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATGGCCCGCAATGTAACGCGGAA‑‑AAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCg                                                                                                                                                                                                   <  2:272991/231‑1 (MQ=255)                                                            aCAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACAC‑CTTTTGGGGGAAAA‑C‑GACCGCCGACGGGACATTGCTTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAA‑‑AAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTaaa                                                                                                                                                                                     >  2:123918/1‑249 (MQ=255)                                                              aGGTTTTCTGCCATGCCTGCGGTTTTGCCAACAC‑CTTTTGGGGGAAAA‑C‑GACCGCCGACGGGACCTTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAA‑‑AAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGTTATGTTAAAAgcg                                                                                                                                                                                 >  2:166877/1‑251 (MQ=255)                                                                gttttccccccgccctggGGTTTTGCCA‑CCC‑CCTTTGGGGGAAAA‑CCGACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGTGGTCAGGGAGGGTTTGAAGAGGACGACGGTCATCGCGAAAAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAA‑‑AAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAgcggc                                                                                                                                                                               <  2:4061/238‑1 (MQ=255)                                                                gTTTTATGCCCTCCCTGCGGTGTTGCCAACCC‑CTATTGCGGGAAAA‑C‑GACCGCCGACGCGCCGTTGCTTGAACCCTTTCGCCGTCGCTCTCCGGGATGGTTTGAAGATCCCGACGGTCATCGCGAACAATGTGACTTCCGATTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAGCAAC‑‑TATTGCCGCCCGCCGCTGCCGCGCATGGGACACCGTACTGGTTGATCCGGACGCTATGTTAAAAgcggg                                                                                                                                                                               >  2:309745/1‑250 (MQ=255)                                                                   ttCTGCCCTGCCTGCGGTTTTGCCAACAC‑CTTTTGGGGGAAAA‑C‑GACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGCCGAGGGTAATCGGGAACAATGAGAATTCCGTTTCCGATTTAAAAATTGCCCGCAATGGAACGCGGAA‑‑AAAGATATTGACGCACGCCGCTGCCGCGAATGTGACACCGAACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCt                                                                                                                                                                            >  2:283549/1‑251 (MQ=255)                                                                       gcccctgacgtggTTTTGCCAA‑ACACTTTTGGGGGAAAAAC‑GACCGCCGACGGGAAATGGATTGAACCCTTTGGCGGTCGCTGTCAGGGAGGGTTTGAAGATGACGACGTCCACCGCAAAAAATGTGACTTCCTTTTCCGTTTAAAAAATTGCCCTCAATTTAACGCGGAA‑‑AAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACg                                                                                                                                                                         <  2:209455/240‑1 (MQ=255)                                                                        cccgcccggcggttggccaaaccc‑cTTTTGGGGAAAAA‑G‑TGCCGCCGACGGTACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAAAAATGGGACTTCCGTTTCCGTTTTAAAAATGGCCCGCAAGGTAACGCGGAA‑‑AAAGATATTCCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATAGGTTAAAAGCGGCGCTACGAc                                                                                                                                                                       <  1:123918/228‑1 (MQ=255)                                                                           tgcctgcGGTTTTGCCAACAC‑CTTTTGGGGGAAAA‑C‑GACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAA‑‑AAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTg                                                                                                                                                                     >  2:274651/1‑250 (MQ=255)                                                                                  ggTTTTGCCACCAC‑CTTTTGGGGGAAAA‑C‑GACCGCCGACGGGACATTGCTTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAA‑‑AAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGAcgc                                                                                                                                                             >  1:124070/1‑251 (MQ=255)                                                                                     tttGCCAACAC‑CTTTTGGGGGAAAA‑C‑GACCGCCGCCGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGCTGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAA‑‑AAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTAATGGTTGATCCGGACGATATGTTAAAAGAGGCGCTACGACTGCAAGACGCGCt                                                                                                                                                          >  2:220764/1‑251 (MQ=255)                                                                                              ac‑CTTTTGGGGGAAAA‑C‑GACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAA‑‑AAAGATATTGCCGCCCGCCGCTGCCGCGCATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTCTTACg                                                                                                                                                 >  2:276434/1‑251 (MQ=255)                                                                                                             aa‑C‑GACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGCCGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAA‑‑AAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCTGACGGTATGTTAACAGCGGCGCTCCGACTGACAGACGCGCTGGTATTACGCttacgaggcctgt                                                                                                                                   >  2:28821/1‑239 (MQ=255)                                                                                                                           cGGGACATTGATTGAACACTTTAGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAA‑‑AAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGACCGATATGTTCACAGCGGCGCTACCACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCAACAtt                                                                                                                       >  1:129480/1‑250 (MQ=255)                                                                                                                             ggACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAA‑‑AAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCAACATggg                                                                                                                     >  2:175717/1‑251 (MQ=255)                                                                                                                                 attgattgAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAA‑‑AAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCAACATGGGCacg                                                                                                                 >  1:32355/1‑251 (MQ=255)                                                                                                                                     attgaACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAA‑‑AAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCAACATGGGCacgacg                                                                                                              >  1:260648/1‑250 (MQ=255)                                                                                                                                      ttgaACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAA‑‑AAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCACCATGGGCacgacgcg                                                                                                            >  1:268610/1‑249 (MQ=255)                                                                                                                                              tttggcagtcgCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGCCTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTACCGCGGAA‑‑AAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTCAAAAGCGGCGCTACGACTGACAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCAACATGGGCCCGTCGAGAAAGGCgc                                                                                                    >  2:35011/8‑250 (MQ=255)                                                                                                                                                  gccgtcgCTGTCGGGGATGGTTTGAAGATGACGACGGTCAGCGCGAACAATGTGACTTCCGTTTCGGTTTTAAAAATTGCCCGCAATGTAACGGGGAA‑‑AAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCAACATGGGCACGACGAGAAAGGCGAATgg                                                                                                <  1:294141/249‑1 (MQ=255)                                                                                                                                                   ccgtcgCTTTCAGGGATGGTTTGAAGATGGCGGCGGTCATCGCGAACAATGTGACTTCCGTTTCCTTTTTAAAAATGGCCCGAAATGTAACGCGGAA‑‑AAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCAACATGGGCACGACGAGAAAGGCGAATGGt                                                                                               <  1:21218/250‑1 (MQ=255)                                                                                                                                                             cAGGTATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTTCGTTCCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAA‑‑AAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCAACATGGGCACGACGAGAAAGGCGAATGGTTGAAAATCAc                                                                                     <  1:15000/251‑1 (MQ=255)                                                                                                                                                             cAGGGATGGTTGTAAGATGACGACGGTCATCGCAAACAATGTGCCTTCCGTTTCCGTTTTAAAATTGGCCCGCAATGTAACGCGGAA‑‑AAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCGACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCAACATGGGCACGACGAGAAAGGCGAATGGTTGAAAATCa                                                                                      <  1:220764/250‑1 (MQ=255)                                                                                                                                                                         gAAGATGACGACGGTCATCGCAAACAATGTGACTTCCGTTTCTGTTTTAAAATTTGCCCGCAATTTAACGCGGAA‑‑AAAAATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGTCTGAAAGACGCGCTGGTATTACGCTGTAGCGGCAGGTCTTTGCAACATGGGCACGACGAGAAAGGCGAATGGTTGAAAATCACCTATTACGATg                                                                          <  1:29836/250‑1 (MQ=255)                                                                                                                                                                           aGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAA‑‑AAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCAACATGGGCACGACGAGAAAGGCGAATGGTTGAAAATCACCTATTACGATGAAg                                                                       <  1:119152/251‑1 (MQ=255)                                                                                                                                                                            gATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAA‑‑AAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCAACATGGGCACGACGAGAAAGGCGAATGGTTGAAAATCACCTATTACGATGa                                                                         >  1:51930/1‑248 (MQ=255)                                                                                                                                                                                  gacgGTCATCACGACCAATGTGAATTACGTCTCCGTTTTAAAAATTGCCCGAAATGTGACGCGGAA‑‑AAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGCTCCGGACGATATGTTAAAAGCGGCGCCACGACTGAAAGACGCGCTGGCATAACGATGAAGCGGCATGTCTTTGCAAAATGGGCACGACGAGAAAGGAGAATAGTTGAAAATACCCCATTACGCTGCAGACGgcgg                                                                >  2:143025/1‑250 (MQ=255)                                                                                                                                                                                              aaaCAATGAGACTTCCGTTTCCGTTTTAAAAAGTGCGCGAAATGAAACGGGAAA‑‑AAAGATATGGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCAACATGGGCACGACGAGAAAGGCGAATGGTTGAAAATCACCTATTACGATGAAGACGGCGCGGATgtgagtga                                                    <  2:78867/250‑1 (MQ=255)                                                                                                                                                                                                                   ccgatcaaaaaTTGCCCGCAATGTAACGCGAAA‑‑AAAGATATTGCCCCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCAACATGGGCACGACGAGAAAGGCGAATGGTTGAAAATCACCTATTACGATGAAGACGGCGCGGATGTGAGTGAGCGTTTCCGTCTGCAAacac                                <  2:24040/244‑1 (MQ=255)                                                                                                                                                                                                                          aaaaTTGCCCGCAATGTAACGCGGAA‑‑AAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCAACATGGGCACGACGAGAAAGGCGAATGGTTGAAAATCACCTATTACGATGAAGACGGCGCGGATGTGAGTGAGCGTTTCCGTCTGCAAACAccgatcgg                         >  1:24040/1‑244 (MQ=255)                                                                                                                                                                                                                                      aaTGTAACGGGAAA‑‑AAACATATTGCCGCCCGCCGCTTCCGCGAATTTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTTTAGCGGCATGTCTTTGCAACATGGGCTCGACGAGAAAGGCGAATGGTTGAAAATCACCTATTACGATCAAGGCGGCGCGGATGTGAGTGAGCGTTTCCGTCTGCAAACACCTGCCCAGCGTACCGCCTTc            <  2:118556/251‑1 (MQ=255)                                                                                                                                                                                                                                        tGTAACGCGGAA‑‑AAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCAACATGGGCACGACGAGAAAGGCGAATGGTTGAAAATCACCTATTACGATGAAGACGGCGCGGATGTGAGTGAGCGTTTCCGTCTGCAAACACCTGCCCAGCGTACCGCCTTCgg          >  1:68442/1‑250 (MQ=255)                                                                                                                                                                                                                                          tAACGCGGAA‑‑AAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCAACATGGGCACGACGAGAAAGGCGAATGGTTGAAAATCACCTATTACGATGAAGACGGCGCGGATGTGAGTGAGCGTTTCCGTCTGCAAACACCTGCCCAGCGTACCGCCTTCGagc        >  1:102041/1‑251 (MQ=255)                                                                                                                                                                                                                                            aCGCGGAA‑‑AAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATACGGACGATCTGTTAAAAGCGGCGCTCCGACTGACAGACGCGCTGGTATTACGCTGCAGCGGCATGTCTTTGCAACATGGGCACGGCGAGAAAGGCGAATGGTTGAAAATCACCTAATACGATGAAGACGGCGCGGGGGTGAGTGAGCGTTTCCGTCTGCAAACACCTGCCCAGCGTACCGCATTCGagccg      >  1:19301/1‑249 (MQ=255)                                                                                                                                                                                                                                                ggAA‑‑AAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTAATACGCTGTAGCGGCATGTCTTTGCAACATGGGCACGACGAGAAAGGCGAATGGTTGAAAATCACCTATTACGATGAAGACGGCGCGGATGTGAGTGAGCGTTTCCGTCTGCAAACACCTGCCCAGCGTACCGCCTTCGAGCAGCttt  >  2:138912/1‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                     CACGATCTCTACGCGCCGGAAGTTGGTACACCAAAAGGCAAAAGTGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACAC‑CTTTTGGGGGAAAA‑C‑GACCGCCGACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAA‑‑AACGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCAACATGGGCACGACGAGAAAGGCGAATGGTTGAAAATCACCTATTACGATGAAGACGGCGCGGATGTGAGTGAGCGTTTCCGTCTGCAAACACCTGCCCAGCGTACCGCCTTCGAGCAGCTTT  >  NC_000913/2281712‑2282198

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑