Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,474,424	T→G	K43N (AAA→AAC)	rpsL ←	30S ribosomal subunit protein S12

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,474,424	0	T	G	100.0%	187.8 / NA	49	K43N (AAA→AAC)	rpsL	30S ribosomal subunit protein S12
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (18/31);  total (18/31)

AGCCTTAGGACGCTTCACGCCATACTTGGAACGAGCCTGCTTACGGTCTTTAACGCCGGAGCAGTCAAGCGCACCACGTACGGTGTGGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAGGACGCCGAATTTTAGGGCGATGCCGAAAAGGTGTCAAGAAATATACAACGA  >  NC_000913/3474181‑3474669                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                      aGCCTTAGGTCGCTTCACGCCATTCTTGGAACGAGCCTGCTTACGGCCTTTAACGCCTGGGCATTCATGCGCACCACGTACGGTGTGGTAACGAACACCCGGGAGGTCTTTAACACGTCCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTGTACGCAGCGCGGAGTTCGGTTTGTTAGGAg                                                                                                                                                                                                                                                <  1:296392/251‑1 (MQ=255)   ccTTAGGACGCTTCACGCCATACTTGGAACGAGCCTGCTTACGGTCTTTAACGCCGGAGCAGTCAAGCGCACCACGTACGGTGTGGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAGGAGTg                                                                                                                                                                                                                                              >  1:153326/1‑251 (MQ=255)   ccTTAGGACGATTCACGCCATACTTGGAACGAGCCTGCTTACGGTCTTTAACGCCGGAGCAGTCAAGCGCACCACGTACGGTGTGGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAGGAGTg                                                                                                                                                                                                                                              >  1:87823/1‑251 (MQ=255)      tAGGACGCTGCACCCCATACTTGGAACGGGCCTGCTTAGGTTTTTTAACCCCGGAGCAGTCAAGCGCACCACGGACGGTGTGGTAACGAACACCCGGGAGGTTTTTAACCCGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTATTAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTTCGCATCGCGGAGGTCGGTTGGTTAGGAGTGgt                                                                                                                                                                                                                                            <  2:194824/250‑1 (MQ=255)                 aCGCCATACTTGGAACGAGCCTGCTTACGGTCTTTAACGCCGGAGCAGTCAAGCGCACCACGTACGGTGTGGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAGGAGTGGTAGTATATACACg                                                                                                                                                                                                                                >  2:62417/1‑251 (MQ=255)                                  aGCCTGCTTACGGTCTTTAACGCCGGAGCAGTCAAGCGCACCACGTACGGTGTGGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGtt                                                                                                                                                                                                               >  2:204309/1‑251 (MQ=255)                                         ttACGGTCTTTAACGCCGGAGCAGTCAAGCGCACCACGTACGGTGTGGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCgg                                                                                                                                                                                                        >  1:54512/1‑251 (MQ=255)                                           aCGGTCTTTAACGCCGGAGCAGTCAAGCGCACCACGTACGGTGTGGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCggg                                                                                                                                                                                                       <  1:276008/250‑1 (MQ=255)                                            cGGTCTTTAACGCCGGAGCAGTCAAGCGCACCACGTACGGTGTGGTAACGAACACCCGGGAGGTCTTTAACACGCCCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGc                                                                                                                                                                                                      >  2:45087/1‑250 (MQ=255)                                                        ccGGAGCAGTCAAGCGCACCACGTACGGTGTGGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAgcgc                                                                                                                                                                                         >  2:56554/1‑251 (MQ=255)                                                              cAGTCAAGCGCACCACGTACGGTGTGGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCAc                                                                                                                                                                                   <  1:65079/251‑1 (MQ=255)                                                                       cccccacGTACGGTGGGGTAACGAACACCCGGGAGGTCTGTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAGGAGTGGTAGTATAGACACGAGTACAGACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCTCGtgtctttt                                                                                                                                                                          <  2:44325/248‑8 (MQ=255)                                                                        ccccacGTACGGTGTGGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTc                                                                                                                                                                         <  2:87823/249‑1 (MQ=255)                                                                        caccacGTACGGTGGGGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTc                                                                                                                                                                         <  2:153326/251‑1 (MQ=255)                                                                         accacGTACGGTGTGGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAGGAGTGGTAGTATATACACGAGTACATCCGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCg                                                                                                                                                                        >  1:121024/1‑251 (MQ=255)                                                                             cGTACGGTGTGGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAAc                                                                                                                                                                    >  1:193913/1‑251 (MQ=255)                                                                                ccGGTGTGGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACttt                                                                                                                                                                 <  1:207988/250‑1 (MQ=255)                                                                                              aaCACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCTCAACt                                                                                                                                                                   <  2:52933/235‑1 (MQ=255)                                                                                              aaCACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACt                                                                                                                                                                   >  1:52933/1‑235 (MQ=255)                                                                                              aaCACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGtt                                                                                                                                                   >  1:37796/1‑251 (MQ=255)                                                                                                     gggAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTAc                                                                                                                                            >  1:47366/1‑251 (MQ=255)                                                                                                       gAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAGGAGTg                                                                                                                                                                                                                                              >  2:239555/1‑151 (MQ=255)                                                                                                       gAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAGGAGTg                                                                                                                                                                                                                                              <  1:239555/151‑1 (MQ=255)                                                                                                           tCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGGTCGGTTTGTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTg                                                                                                                                      >  1:132812/1‑251 (MQ=255)                                                                                                           gcgttAACGCGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCTCAGGCTCGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTTCGTACCAGCTg                                                                                                                                      <  1:287695/248‑1 (MQ=255)                                                                                                              ttAACACGACCGCCACGGATCAGGATCGCGGAGGTCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACGTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTGAGGAGTGGTAGTATATACACGAGTACATACGCCTCGTTTGTGCGGGCATGCTTCCAGCGCAGGCACGGTGCTTTTCGCAACTTTTCGAGCACGTGGTTTGCTTACCAGCTg                                                                                                                                      <  2:136872/248‑1 (MQ=255)                                                                                                               tAACGCGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGtt                                                                                                                                   <  1:290731/250‑1 (MQ=255)                                                                                                                aacccGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCGCCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTaa                                                                                                                                 <  2:95119/247‑1 (MQ=255)                                                                                                                aaCACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTa                                                                                                                                  <  1:295647/250‑1 (MQ=255)                                                                                                                aaCACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGAACTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTa                                                                                                                                  >  1:289673/1‑250 (MQ=255)                                                                                                                   ccGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGGCCTTCACCACCGATGTAGGAAGTCTCTTCGAAGCCGTTAGGCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTGTGTTAGGAGTGGTAGTATAGACACGAGTACATACGCCACGTTTTTTCGGGCATGCTTCCTGCGCAGGCACGTTGTTTTGCGCAACGTTGCGAGCACGTGGTTGTCGTACCAGCTGGTTAACTg                                                                                                                              <  2:317465/250‑1 (MQ=255)                                                                                                                         gCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCa                                                                                                                        <  2:67203/251‑1 (MQ=255)                                                                                                                          ccACGGATCAGTATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCTCCGATGTAGGAAGTCACTTCGAAGTCGTTAGTCAGACGAACACGGCATTCTTTACGCAGCGCGGAGTTCGGTTTGTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCATGCACGTTGCTTTTCGCATCTTTGCGAGCACGTGGTTTGCGTACCAGCGGGTTAACTGTTGCCAt                                                                                                                       <  2:240275/251‑1 (MQ=255)                                                                                                                             cGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTaa                                                                                                                    <  1:184418/251‑1 (MQ=255)                                                                                                                                atcaggatcaCGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGCTGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAGGAGTGGTAGTCTATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAAt                                                                                                                  >  1:16762/1‑250 (MQ=255)                                                                                                                                atcaggatcaCGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGCTGCCATTAAATa                                                                                                                 <  2:172725/251‑1 (MQ=255)                                                                                                                                      atcaCGGAGGGCTCCTGCAGGTTGTGCCCTGCACCACCGATGTAGGAAGTCACTTCGAAACCGTGAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAGGAGTGGTAGTATATACACGAGTACATACTCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCTAGCACGTGGTTGGCGTACCAGCTGGTTAACTGTTGCCATGAAATAGCTCCt                                                                                                           <  2:121024/251‑1 (MQ=255)                                                                                                                                            gAGTGCTCCTGCAGGTTGTGACCTTCACCACCGAGGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGTAGTTCGGTTTGTTAGGAGTGGTAGTATATACGCGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGtttt                                                                                                     <  2:174455/251‑1 (MQ=255)                                                                                                                                                   ccTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACTGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCtttt                                                                                              <  2:37955/251‑1 (MQ=255)                                                                                                                                                         ggTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCg                                                                                        <  1:62417/251‑1 (MQ=255)                                                                                                                                                                          ccGATGTAGGAATGCACTGCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCTCGGAGTTCGGTTTGTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCTGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAAc                                                                       <  2:298221/251‑1 (MQ=255)                                                                                                                                                                                               aaaCCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACGGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAGGAc                                                  <  2:300842/251‑1 (MQ=255)                                                                                                                                                                                                          cAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAGGACGCCGAATTTTa                                       <  1:56554/251‑1 (MQ=255)                                                                                                                                                                                                          aaGACGAACACCGCATACTTTACGAAGCGCGGAGTTCGGTTTGTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTACTAAAACTCCCTCACGCAATATGAGGACGCCGACtttt                                        >  2:14635/2‑250 (MQ=255)                                                                                                                                                                                                            gACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAGGACGCCGAATTTTAgg                                     <  2:323434/251‑1 (MQ=255)                                                                                                                                                                                                                   ccGGCATACTTTACGCAGCGCGGAGTTCGGTTTGTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAGGACGCCGAATTTTAGGGCGATGc                              <  1:6706/250‑1 (MQ=255)                                                                                                                                                                                                                                  cAGCGCGGAGTTCGGTTTGTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGATTTTCGCCACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACCATATGAGGACGCCGAATTTTAGGGCGATGCCGAAAAGGTGTCa                 >  1:137150/1‑249 (MQ=255)                                                                                                                                                                                                                                         gAGTTCGGTTTGTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATTCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGTTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAGGACGCCGAATTTTAGGGCGATGCCGAAAAGGTGTCAAGAAAtata        <  1:219655/251‑1 (MQ=255)                                                                                                                                                                                                                                               ggTTTGTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAGGACGCCGAATTTTAGGGCGATGCCGAAAAGGTGTCAAGAAATATACAACGa  <  2:312084/251‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                      AGCCTTAGGACGCTTCACGCCATACTTGGAACGAGCCTGCTTACGGTCTTTAACGCCGGAGCAGTCAAGCGCACCACGTACGGTGTGGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAGGACGCCGAATTTTAGGGCGATGCCGAAAAGGTGTCAAGAAATATACAACGA  >  NC_000913/3474181‑3474669

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑