Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,719,429	G→A	intergenic (‑385/‑49)	yiaF ← / → yiaG	DUF3053 domain‑containing protein YiaF/putative DNA‑binding transcriptional regulator YiaG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,719,429	0	G	A	100.0%	78.0 / NA	24	intergenic (‑385/‑49)	yiaF/yiaG	DUF3053 domain‑containing protein YiaF/putative DNA‑binding transcriptional regulator YiaG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/12);  total (12/12)

GTTGTTTCATTGTTAAAAAATGATAGTGCCGTTGCCTTTTTTGTAGCCAGAGATTTACGCCTTCGCAGAAGGTTAGATTTTCGAAATCTTTACAAAACAGGAGTGGTAAGAGATATATCGGCATTTATCAGATCGTTAGGTGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATGAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTGTTGAGCAGCCTGGAACAGATTGTTTTTAAAGATGAAACGCAGAAAATTACCCTGACGCACAGAACAACGTCCTGTACCGAAATTGAGCAGTTACGAAAAGGGACAGGATTAAAAATCGATGATTTCGCCC  >  NC_000913/3719190‑3719637                                                                                                                                                                                                                                                |                                                                                                                                                                                                                 gttgttTCATTGTTAAAAAATGATAGTGCCGTTGCCTTTTTTGTAGCCAGAGATTTACGCCTTCGCAGAAGGTTAGATTTTCGAAATCTTTACAAAACAGGAGAGGTAAGAGATATATCGGCATTTATCAGCTCGTTAGGTGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTAAATAAGCTTCGATGc                                                                                                                                                                                                       >  1:108272/1‑251 (MQ=255)        cATTGTTAAAAAATGATAGTGCCGTTGCCTTTTTTGTAGCCAGAGATTTACGCCTTCGCAGAAGGTTAGATTTTCGAAATCTTTACAAAACAGGAGTGGTAAGAGATATATCGGCATTTATCAGATCGTTAGGTGTCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCGGCTGTGTCTGCTTTGCCCGACTATTCTTAAGAAGCTTCGATGCAATTCAc                                                                                                                                                                                                <  1:124386/251‑1 (MQ=255)           tattaacaaaagacagtgactttGCATTTTTTGTAGCCAGTGATTTACACCTTCGCAGAAGGTTAGATTTTCGAAATCTTTACAATACAGGAGTGGTAAGAGATATATCGGCATTTATCAGATCGTTAGGTGGCTATAAGTCACGCTAAATGACATTCTGAATCGAATAATAGCCTGAGCATGCCATGACTTCACCCCGCTGTGTTTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGTAATTTACGa                                                                                                                                                                                              >  2:98681/22‑250 (MQ=255)                  aaaTGATAGTGCCGTTGCCTTTTTTGTAGCCAGAGATTTACGCCTTCGCAGAAGGTTAGATTTTCGAAATCTTTACAAAACAGGAGTGGTAAGAGATATATCGGCATTTATCAGATCGTTAGGTGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAg                                                                                                                                                                                      <  1:12126/251‑1 (MQ=255)                      gATAGTGCCGTTGCCTTTTTTGTAGCCAGAGATTTACGCCTTCGCAGAAGGTTAGATTTTCGAAATCTTTACAAAACAGGAGTGGTAAGAGATATATCGGCATTTATCAGATCGTTAGGTGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCCGtgtg                                                                                                                                                                                  >  2:243581/1‑251 (MQ=255)                             ccGTTGCCTTTTTTGTAGCCAGAGATTTACGCCTTCGCAGAAGGTTAGATTTTCGAAATCTTTACAAAACAGGAGTGGTAAGAGATATATCGGCATTTATCAGATCGTTAGGTGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGGCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGCTTTg                                                                                                                                                                             >  2:168052/1‑249 (MQ=255)                                              gCCAGAGATTTACGCCTTCGCAGAAGGTTAGATTTGCGAAATCATTACAAAACAGGAGTGGTAAGAGATATATCGGCATTTATCAGATCGTTAGGAGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTGCGATGCAATTCACGATCCCGCAGTGTGATTTGAAGAGTTTTCAATGGAata                                                                                                                                                          <  2:265733/251‑1 (MQ=255)                                                            ccTTCGCAGAAGGTTAGATTTTCGAAATCTTTACAAAACAGGAGTGGTAAGAGATATATCGGCATTTATCAGATCGTTAGGTGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGc                                                                                                                                            >  1:253141/1‑251 (MQ=255)                                                               tCGCAGAAGGTTAGATTTTCGAAATCTTTACAAAACAGGAGTGGTAAGAGATATATCGGCATTTATCAGATCGGTAGGTGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCa                                                                                                                                           <  2:19039/249‑1 (MQ=255)                                                                                        cTTTACAAAACAGGAGTGGTAAGAGATATATCGGCATTTATCAGATCGTTAGGTGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTGTTGAGCAGCCTGGAAc                                                                                                                    >  1:254589/1‑247 (MQ=255)                                                                                                     gAGTGGTAAGAGATATATCGGCATTTATCAGATCGTTAGGTGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTGTTGAGCAGCCTGGAACAGATTGTTTTTAAAGAt                                                                                                   <  1:58995/251‑1 (MQ=255)                                                                                                                   atatCGGCATTTATCAGATCGTTAGGTGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGtt                                                                                                                                                                      >  2:35035/1‑170 (MQ=255)                                                                                                                   atatCGGCATTTATCAGATCGTTAGGTGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGtt                                                                                                                                                                      <  1:35035/170‑1 (MQ=255)                                                                                                                    tatCGGCATTTATCAGATCGTTAGGTGGCTATAAGTCACGCTAAATGCCAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTGTTGAGCAGCCTGGAACAGATTGTTTTTAAAGATGAAACGCAGAACAtt                                                                                    >  2:274806/1‑251 (MQ=255)                                                                                                                       cGGCATTTATCAGATCGTTAGGTGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTGTTGAGCAGCCTGGAACAGATTGTTTTTAAAGATGAAACGCAGAAAATTAcc                                                                                 >  2:131481/1‑251 (MQ=255)                                                                                                                                                        cACGCGAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTGTTGAGCAGCCTGGAACAGATTGTTTTTAAAGATGAAACGCAGAAAATTACCCTGACGCACAGAACAACGTCCTGTACCGAAAt                                                 <  2:77497/250‑1 (MQ=255)                                                                                                                                                         aCGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTGTTGAGCAGCCTGGAACAGATTGTTTTTAAAGATGAAACGCAGAAAATTACCCTGACGCACAGAACAACGTCCTGTACCGAAATTg                                               >  1:112370/1‑251 (MQ=255)                                                                                                                                                              aaaTGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTGTTGAGCAGCCTGGAACAGATTGTTTTTAAAGATGAAACGCAGAAAATTACCCTGACGCACAGAACAACGTCCTGTACCGAAATTGAGc                                            <  1:131481/249‑1 (MQ=255)                                                                                                                                                                   aCAGGCTGAATCGAATAATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTCATACGCTTCGATGCAATTCACGAACCCGCAGTGAGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTGTTGAGCAGCCTGGAACAGCTTGTTTTTAAAGATGAAACGCAGAAAATTACCCAGCCGCACAGAAACACGTGCTGTACCGAAATTGAGCAGCTAcc                                     >  2:150854/1‑250 (MQ=255)                                                                                                                                                                               gaatcATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTGTTGAGCAGCCTGGAACAGATTGTTTTTAAAGATGAAACGCAGAAAATTACCCTGACGCACAGAACAACGTCCTGTACCGAAAATGAGCAGTTACGAAAAGGGACAgg                         <  2:227640/251‑1 (MQ=255)                                                                                                                                                                                   cATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATGCACGATCCCGCAGTGTGATTGGAGGAGTGTTCAATGGAATATAAAGATCCAATGCATGAGCTGTTGAGCAGCCTGTAACAGATTGTTTTTAAAGATGAAACGCAGAAAATTACCCTGACGCACAGAACAACGTCCTGTACCGAAATTGAGCAGTTACGAAAAGGGACAGGATTa                     <  1:47490/251‑1 (MQ=255)                                                                                                                                                                                    aTAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCGTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTGTTGAGCAGCCTGGAACAGATTGTTTTTAAAGATGAAACGCAGAAAATTACCCTGACGCACAGAACAACGTCCTGTACCGAAATTGAGCAGTTACGAAAAGGGACAGGATTaa                    <  1:326055/251‑1 (MQ=255)                                                                                                                                                                                                   ccTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTGTTGAGCAGCCTGGAACAGATTGTTTTTAAAGATGAAACGCAGAAAATTACCCTGACGCACAGAACAACGTCCTGTACCGCAATTGAGCAGTTACGAAAAGGGACAGGCTTAAACATCGATGATTTCg     >  2:221751/1‑251 (MQ=255)                                                                                                                                                                                                      gACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTGTTGAGCAGCCTGGAACAGATTGTTTTTAAAGATGAAACGCAGAAAATTACCCTGACGCACAGAACAACGTCCTGTACCGAAATTGAGCAGTTACGAAAAGGGACAGGATTAAAAATCGATGATTTCGccc  <  2:1300/251‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                 GTTGTTTCATTGTTAAAAAATGATAGTGCCGTTGCCTTTTTTGTAGCCAGAGATTTACGCCTTCGCAGAAGGTTAGATTTTCGAAATCTTTACAAAACAGGAGTGGTAAGAGATATATCGGCATTTATCAGATCGTTAGGTGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATGAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTGTTGAGCAGCCTGGAACAGATTGTTTTTAAAGATGAAACGCAGAAAATTACCCTGACGCACAGAACAACGTCCTGTACCGAAATTGAGCAGTTACGAAAAGGGACAGGATTAAAAATCGATGATTTCGCCC  >  NC_000913/3719190‑3719637

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑