Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,281,855	T→C	G408G (GGT→GGC)	radD →	putative DNA repair helicase RadD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,281,855	0	T	C	100.0%	91.8 / NA	31	G408G (GGT→GGC)	radD	putative DNA repair helicase RadD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/12);  total (19/12)

GCCCTACCGAATCAGTGAGTCTTTACCAACAAATTGTCGGGCGCGGTCTGCGTCTCGCTCCGGGCAAGACTGATTGCTTAATTCTTGATTATGCGGGTAATCCTCACGATCTCTACGCGCCGGAAGTTGGTACACCAAAAGGCAAAAGTGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACAC‑CTTTTGGGGGAAAA‑C‑GACCGCCGACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAA‑‑AACGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCAACATGGGCACGACGAG  >  NC_000913/2281608‑2282092                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                ccccTACCGAATCAGTGAGTCTTTACCAACAAATTGTCGGGCGCGGTCTGCGTCTCGCTCCGGGCAAGACTGATTGCTTAATTCTTGATTATGCGGGTAATCCTCACGATCTCTACGCGCCGGAAGTTGGTACACCAAAAGGCAAAAGTGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACAC‑CTTTTGGGGGAAAA‑C‑GACCGCCGACGGGACATTGATTGAACACTTTGgccgt                                                                                                                                                                                                                                              <  1:286050/250‑1 (MQ=255)                tGAGTCTTTACCAACAAATTGTCGGGCGCGGTCTGCGTCTCGCTCCGGGCAAGACTGATTGCTTAATTCTTGATTATGCGGGTAATCCTCACGATCTCTACGCGCCGGAAGTTGGTACACCAAAAGGCAAAAGTGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACAC‑CTTTTGGGGGAAAA‑C‑GACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATgg                                                                                                                                                                                                                               >  1:292932/1‑251 (MQ=255)                         aCCAACAAATTGTCGGGCGCGTTTTGCGTCTCGTTCCGGGCAAGACTGATTGCTTAATTCTTGATTAGGCGGGTAATCCTCACGATCTCTACGCGCCGGAAGTTGGTACACCAAAAGGCAAAAGTGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACAC‑CTTTGGGGGGAAAA‑C‑GACCGCTGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGAGGGTTTGAAGa                                                                                                                                                                                                                       <  2:310947/250‑1 (MQ=255)                           caacaaATTTTCGGGCGCGGTCTGCGTCTCGCTCCGGGCAAGACTGATTGCTTAATTCTTGATTATGCGGGTAATCCTCACGATCTCTACGCGCCGGAAGTTGGTACCCCAAAAGGCAAAAGTGACAACGTTCCGGTACAGGTTGTCTGCCCTGCCTGCGGTTTTGCTAACAC‑CTTTTGGGGGAAAA‑C‑TACCGCCGACGGTACATTGATTGAACACGTTGGCCGTCGCTGTTATGGATGGTTTGAAGATga                                                                                                                                                                                                                    <  2:216642/251‑1 (MQ=255)                                             ggtttGCGTCTCGCTCCGGGCAAGACTGATTGCTTAATTCTTGATTATGCGGGTAATCCTCACGATCTCTACGCGCCGGAAGTTGGTACACCAAAAGGCAAAAGTGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACAC‑CTTTTGGGGGAAAA‑C‑GACCGCCGACGGGACATTGATTGAACACGTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACTGTCATCGCGAACa                                                                                                                                                                                                  <  2:185958/247‑1 (MQ=255)                                                    gactcGCTCCGGGCAAGACTGATTGATTAATTCTTGATTATGCGGGTCATCCTCACGATCTCTACGCGCCGGTAGTTGGTACACCAAAAGGCAAAAGTGACAACGTTCCGGAACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACAC‑CTTTTGGGGGAAAA‑C‑GACCGCCGACGGGACATTGATTGCACAATTTGGCCGTCGCTGTCAGGGATGGTTTGAAGTTGACGACGGTCATCGCGAACAATGTGAc                                                                                                                                                                                           >  2:235413/3‑251 (MQ=255)                                                        cGCTCCGGGCAAGACTGATTGCTTAATTCTTGATTATGCGGGTAATCCTCACGATCTCTACGCGCCGGAAGTTGGTACACCAAAAGGCAAAAGTGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACAC‑CTTTTGGGGGAAAA‑C‑GACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACttcc                                                                                                                                                                                       <  2:56357/251‑1 (MQ=255)                                                            ccGGGCAAGACTGATTGCTTAATTCTTGATTATGCGGGTAATCCGCACGATCTCTACGCGCCGGAAGTTGGTACACCAAAAGGCAAAAGTGACAACGTTCCGGTACATGTTTTCTGCCCTGCCTGCGGTTTTGCCAACGC‑CTTTTGGGGGAAAA‑C‑GACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAAGGTGACttccgttt                                                                                                                                                                                   <  1:268825/251‑1 (MQ=255)                                                                                      tGATTATGCGGGTAATCCTCACGATCTCTACGCGCCGGAAGTTGGTACACCAAAAGGCAAAAGTGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACAC‑CTTTTGGGGGAAAA‑C‑GACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCACGCAATGt                                                                                                                                                          >  2:923/1‑250 (MQ=255)                                                                                              cGGGTAATCCTCACGATCTCTACGCGCCGGAAGTTGGTACACCAAAAGGCAAAAGGGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACAC‑CTTTTGGGGGAAAA‑C‑GACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGaa                                                                                                                                                 <  1:208076/251‑1 (MQ=255)                                                                                                         cACGATCTCTACGCACCGGAGGTTGGTACACCAAAAGGCAAAAGTGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACAC‑CTTTTGGGGGAAAA‑C‑GACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCAGCGCGAACAATGTGACTTCCGTTTCCGTTGTAAAAATTGCCCGCAATGTAACGCGGAA‑‑AAAGATATTgc                                                                                                                                    <  2:315605/251‑1 (MQ=255)                                                                                                                                      acCAACAGGCAAAAGTGACAACGTTCCGGTACAGGGTTTCTGCCCTGCCTGCGGTTTTGCCAACAC‑CTTTTGGGGGAAAA‑C‑GACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAA‑‑AAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCg                                                                                                       >  2:224605/1‑251 (MQ=255)                                                                                                                                                    gtaacaccgttcgggtaggtgTTTTCTCCCCTCCCGGGGGTTTT‑CCAACCC‑CCTTTGGGGGAAAA‑CCGACCGCGGAGGGGACATTGATTGAACCCTTTGGCCGTCGTTGTCAGGGATGGTTTGAAGATGACGACGGTCACCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATGGCCCGCAATGTAACGCGGAA‑‑AAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCg                                                                                         <  2:272991/231‑1 (MQ=255)                                                                                                                                                      gACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACTC‑CTTTTGGGGGAAAA‑C‑GACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAAt                                                                                                                                                                      <  2:52922/174‑1 (MQ=255)                                                                                                                                                      gACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACAC‑CTTTTGGGGGAAAA‑C‑GACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAAt                                                                                                                                                                      >  1:52922/1‑174 (MQ=255)                                                                                                                                                                    aCAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACAC‑CTTTTGGGGGAAAA‑C‑GACCGCCGACGGGACATTGCTTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAA‑‑AAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTaaa                                                                           >  2:123918/1‑249 (MQ=255)                                                                                                                                                                      aGGTTTTCTGCCATGCCTGCGGTTTTGCCAACAC‑CTTTTGGGGGAAAA‑C‑GACCGCCGACGGGACCTTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAA‑‑AAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGTTATGTTAAAAgcg                                                                       >  2:166877/1‑251 (MQ=255)                                                                                                                                                                        gttttccccccgccctggGGTTTTGCCA‑CCC‑CCTTTGGGGGAAAA‑CCGACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGTGGTCAGGGAGGGTTTGAAGAGGACGACGGTCATCGCGAAAAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAA‑‑AAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAgcggc                                                                     <  2:4061/238‑1 (MQ=255)                                                                                                                                                                        gTTTTATGCCCTCCCTGCGGTGTTGCCAACCC‑CTATTGCGGGAAAA‑C‑GACCGCCGACGCGCCGTTGCTTGAACCCTTTCGCCGTCGCTCTCCGGGATGGTTTGAAGATCCCGACGGTCATCGCGAACAATGTGACTTCCGATTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAGCAAC‑‑TATTGCCGCCCGCCGCTGCCGCGCATGGGACACCGTACTGGTTGATCCGGACGCTATGTTAAAAgcggg                                                                     >  2:309745/1‑250 (MQ=255)                                                                                                                                                                           ttCTGCCCTGCCTGCGGTTTTGCCAACAC‑CTTTTGGGGGAAAA‑C‑GACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGCCGAGGGTAATCGGGAACAATGAGAATTCCGTTTCCGATTTAAAAATTGCCCGCAATGGAACGCGGAA‑‑AAAGATATTGACGCACGCCGCTGCCGCGAATGTGACACCGAACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCt                                                                  >  2:283549/1‑251 (MQ=255)                                                                                                                                                                               gcccctgacgtggTTTTGCCAA‑ACACTTTTGGGGGAAAAAC‑GACCGCCGACGGGAAATGGATTGAACCCTTTGGCGGTCGCTGTCAGGGAGGGTTTGAAGATGACGACGTCCACCGCAAAAAATGTGACTTCCTTTTCCGTTTAAAAAATTGCCCTCAATTTAACGCGGAA‑‑AAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACg                                                               <  2:209455/240‑1 (MQ=255)                                                                                                                                                                                cccgcccggcggttggccaaaccc‑cTTTTGGGGAAAAA‑G‑TGCCGCCGACGGTACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAAAAATGGGACTTCCGTTTCCGTTTTAAAAATGGCCCGCAAGGTAACGCGGAA‑‑AAAGATATTCCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATAGGTTAAAAGCGGCGCTACGAc                                                             <  1:123918/228‑1 (MQ=255)                                                                                                                                                                                   tgcctgcGGTTTTGCCAACAC‑CTTTTGGGGGAAAA‑C‑GACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAA‑‑AAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTg                                                           >  2:274651/1‑250 (MQ=255)                                                                                                                                                                                          ggTTTTGCCACCAC‑CTTTTGGGGGAAAA‑C‑GACCGCCGACGGGACATTGCTTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAA‑‑AAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGAcgc                                                   >  1:124070/1‑251 (MQ=255)                                                                                                                                                                                             tttGCCAACAC‑CTTTTGGGGGAAAA‑C‑GACCGCCGCCGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGCTGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAA‑‑AAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTAATGGTTGATCCGGACGATATGTTAAAAGAGGCGCTACGACTGCAAGACGCGCt                                                >  2:220764/1‑251 (MQ=255)                                                                                                                                                                                                      ac‑CTTTTGGGGGAAAA‑C‑GACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAA‑‑AAAGATATTGCCGCCCGCCGCTGCCGCGCATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTCTTACg                                       >  2:276434/1‑251 (MQ=255)                                                                                                                                                                                                                     aa‑C‑GACCGCCGACGGGACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGCCGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAA‑‑AAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCTGACGGTATGTTAACAGCGGCGCTCCGACTGACAGACGCGCTGGTATTACGCttacgaggcctgt                         >  2:28821/1‑239 (MQ=255)                                                                                                                                                                                                                                   cGGGACATTGATTGAACACTTTAGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAA‑‑AAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGACCGATATGTTCACAGCGGCGCTACCACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCAACAtt             >  1:129480/1‑250 (MQ=255)                                                                                                                                                                                                                                     ggACATTGATTGAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAA‑‑AAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCAACATggg           >  2:175717/1‑251 (MQ=255)                                                                                                                                                                                                                                         attgattgAACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAA‑‑AAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCAACATGGGCacg       >  1:32355/1‑251 (MQ=255)                                                                                                                                                                                                                                             attgaACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAA‑‑AAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCAACATGGGCacgacg    >  1:260648/1‑250 (MQ=255)                                                                                                                                                                                                                                              ttgaACACTTTGGCCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAA‑‑AAAGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCACCATGGGCacgacgcg  >  1:268610/1‑249 (MQ=255)                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                GCCCTACCGAATCAGTGAGTCTTTACCAACAAATTGTCGGGCGCGGTCTGCGTCTCGCTCCGGGCAAGACTGATTGCTTAATTCTTGATTATGCGGGTAATCCTCACGATCTCTACGCGCCGGAAGTTGGTACACCAAAAGGCAAAAGTGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACAC‑CTTTTGGGGGAAAA‑C‑GACCGCCGACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAA‑‑AACGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGATATGTTAAAAGCGGCGCTACGACTGAAAGACGCGCTGGTATTACGCTGTAGCGGCATGTCTTTGCAACATGGGCACGACGAG  >  NC_000913/2281608‑2282092

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑