Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,489,491	G→A	L420L (CTG→CTA)	aldA →	aldehyde dehydrogenase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,489,491	0	G	A	92.3%	42.0 / NA	13	L420L (CTG→CTA)	aldA	aldehyde dehydrogenase A
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (4/8);  minor base C (1/0);  total (5/8)
Fisher's exact test for biased strand distribution p-value = 3.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAAAGTGGCGCGCGCAGTAGAAGAAGGGGCGAGAGTGGCGTTCGGTGGCAAAGCGGTAGAGGGGAAAGGATATTATTATCCGCCGACATTGCTGCTGGATGTTCGCCAGGAAATGTCGATTATGCATGAGGAAACCTTTGGCCCGGTGCTGCCAGTTGTCGCATTTGACACGCTGGAAGATGCTATCTCAATGGCTAATGACAGTGATTACGGCCTGACCTCATCAATCTATACCCAAAATCTGAACGTCGCGATGAAAGCCATTAAAGGGCTGAAGTTTGGTGAAACTTACATCAACCGTGAAAACTTCGAAGCTATGCAAGGCTTCCACGCCGGATGGCGTAAATCCGGTATTGGCGGCGCAGATGGTAAACATGGCTTGCATGAATATCTGCAGACCCAGGTGGTTTATTTACAGTCTTAATGAGTGAAAGAGGCGGAGGTTTTTTCCTCCGCCTGTGC  >  NC_000913/1489248‑1489709                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                           aaaaGTGGTGCGCGCAGTAGAAGGAGGGGCGAGAGTGGCGTTCGGTGGCAAAGCGGTATAGGGGAAAGGATATGAGTATCCGCCGACATTGCTGCTGGATGTTCGCCAGGAAATGTCGATTATGCATGAGGAAACCGTTGGCCCGGTGCTGCCAGATGTCGCATTTGACACGCTGGAAGATGCTATCGCAATGGCTTATGACGGTGATTACGGCCTGACCTCATCAATCTATACCCAAAATCTAAACGTcg                                                                                                                                                                                                                     <  1:318543/251‑1 (MQ=255)    aGTGGCGCGCGCAGTAGAAGCAGGGGCGAGAGTGGCGTTCGGTGGCAAAGCGGTAGAGGGGAAAGGATATTATTATCCGCAGAAAATGCTGCTGGATGTTCGCCAGGAAATGTCGGTTATGCATGAGGGAAACTTTGGCCAGGTGCTGCCAGTTGTCGCATTTGACACGCTGGAAGATGCTATCTCAATGGCTAATGACAGGGATTACAGCCCTACCTCATCAATCTATACCCAAAATCTCAACATcgcgg                                                                                                                                                                                                                  >  2:221285/1‑250 (MQ=255)       ggCGCGCGCAGTAGAAGAAGGGGCGAGAGTGGCGTTCGGTGGCAAAGCGGTAGAGGGGAAAGGATATTATTATCCGCCGACATTGCTGCTGGATGTTCGCCAGGAAATGTCGATTATGCATGAGGAAACCTTTGGCCCGGTGCTGCCAGTTGTCGCATTTGACACGCTGGAAGATGCTATCTCAATGGCTAATGACAGTGATTACGGCCTGACCTCATCAATCTATACCCAAAATCTAAACGTCGCGATGa                                                                                                                                                                                                               <  1:235121/251‑1 (MQ=255)                           gggCGAGAGTGGCGTTCGGTGGCAAAGCGGTAGAGGGGAAAGGATATTATTATCCGCCGACATTGCTGCTGGATGTTCGCCAGGAAATGTCGATTATGCATGAGGAAACCTTTGGCCCGGTGCTGCCAGTTGTCGCATTTGACACGCTGGAAGATGCTATCTCAATGGCTAATGACAGTGATTACGGCCTGACCTCATCAATCTATACCCAAAATCTAAACGTCGCGATGAAAGCCATTAAAGGGCTGa                                                                                                                                                                                             >  1:192247/1‑249 (MQ=255)                                            ggTGGCAAAGCGGTAGAGGGGAAAGGATATTATTATCCGCCGACATTGCTGCTGGATGTTCGCCAGGAAATGTCGATTATGCATGAGGAAACCTTTGGCCCGGTGCTGCCAGTTGTCGCATTTGACACGCTGGAAGATGCTATCTCAATGGCTAATGACAGTGATTACGGCCTGACCTCATCAATCTATACCCAAAATCTAAACGTCGCGATGAAAGCCATTAAAGGGCTGAAGTTTGGTGAAACTTACAt                                                                                                                                                                          >  2:255966/1‑251 (MQ=255)                                                                         ttattatCCGCCGACATTGCTGCTGGATGTTCGCCAGGAAATGTCGATTATGCATGAGGAAACCTTTGGCCCGGTGCTGCCAGTTGTCGCATTTGACACGCTGGAAGATGCTATCTCAATGGCTAATGACAGTGATTACGGCCTGACCTCATCAATCTATACCCAAAATCTAAACGTCGCGATGAAAGCCATTAAAGGGCTGAAGTTTGGTGAAACTTACATCAACCGTGAAAACTTCGAAGCTATGCa                                                                                                                                               >  1:268240/1‑249 (MQ=255)                                                                                ccgccgACATTGCTGCTGGATGTTCGCCAGGAAATGGCGATTATGCATGAGGAAACCTTTGGCCCGGTGCTGCCAGTTGTCGCATTTGACACGCTGGAAGATGCTATCTCAATGGCTAATGACAGTGATTACGGCCTGACCTCATCAATCTATACCCAAAATCTAAACGTCGCGATGAAAGCCATTAAAGGGCTGAAGTTTGGTGAAACTTACATCAACCGTGAAAACTTCGAAGCTATGCAAGGCTTcc                                                                                                                                       <  2:192247/250‑1 (MQ=255)                                                                                                            aGGAAATGTCGATTATGCATGAGGAAACCTTTGGCCCGGTGCTGCCAGTTGTCGCATTTGACACGCTGGAAGATGCTATCTCAATGGCTAATGACAGTGATTACGGCCTGACCTCATCAATCTATACCCAAAATCTAAACGTCGCGATGAAAGCCATTAAAGGGCTGAAGTTTGGTGAAACTTACATCAACCGTGAAAACTTCGAAGCTATGCAAGGCTTCCACGCCGGATGGCGTAAATCCGGGATTggc                                                                                                          <  1:255966/251‑1 (MQ=255)                                                                                                                                   gAAACCTTGGGCCCGGTGCTGCCAGTTGTCGCATTTGACACGCTGGAAGATGCTATCTCAATGGCGAATGACAGTGATTACGGCCTGACCTCATCAATCTATACCCAAAATCTAAACGTCGCGATGAAAGCCATTAAAGGGCTGAAGTTTGGTGAAACTTACATCAACCGTGAAAACTTCGAAGCTATGCAAGGCTTCCACACCGGATGGCGTAAATCCGGTATTGGCGGCGCAGATGGTAAACATGGCtt                                                                                   <  2:27307/251‑1 (MQ=255)                                                                                                                                                      tgcCAGTTGTCGCATTTGACAAGCTGGAAGATGCTATCTCAATGGCTAATGACAGTGATTACGGCCTGAACTCATCAATCTATACCCACAATCTAAACGTCGCGATGAAAGCCATTAAAGGGCTGAAGTTTGGTGAAACTTACATCAACCGTGAAAACTTCGAAGCTATGCAAGGCTTCCACGCCGGATGGCGTAAATCCGGTATTGGCGGCGCAGATGGTAAACATGGCTTGCATGAATATCTGCAGAcc                                                                >  1:272628/1‑251 (MQ=255)                                                                                                                                                                      tGACACGCTGGAAGATGCTATCTCAATGGCTAATGACAGTGATTACGGCCTGACCTCATCAATCTATACCCAAAATCTAAACGTCGCGATGAAAGCCATTAAAGGGCTGAAGTTTGGTGAAACTTACATCAACCGTGAAAACTTCGAAGCTATGCAAGGCTTCCACGCCGGATGGCGTAAATCCGGTATTGGCGGCGCAGATGGTAAACATGGCTTGCATGAATATCTGCAGACCCAGGTGGtttattta                                                 <  1:197181/250‑1 (MQ=255)                                                                                                                                                                                                        gACAGTGATTACGGCCTGACCTCATCAATCTATACCCAAAATCTAAACGTCGCGATGAAAGCCATTAAAGGGCTGAAGTTTGGTGAAACTTACATCAACCGTGAAAACTTCGAAGCTATGCAAGGCTTCCACGCCGGATGGCGTAAATCCGGTATTGGCGGCGCAGATGGTAAACATGGCTTGCATGAATATCTGCAGACCCAGGTGGTTTATTTACAGTCTTAATGAGTGAAAGAGTCGGAGGtttttt               <  1:102745/250‑1 (MQ=255)                                                                                                                                                                                                                    ggCCTGACCTCATCAATCTATACCCAAAATCTAAACGGCGCGATGAAAGCCATTAAAGGGCTGAAGTTTGGTGAAACTTACATCAACCGTGAAAACTTCGAAGCTATGCAAGGCTTCCACGCCGGATGGCGTAAATCCGGTATTGGCGGCGCAGATGGTAAACATGGCTTGCATGAATATCTGCAGACCCAGGTGGTTTATTTACAGTCTTAATGAGTGAAAGAGGCGGAGGTTTTTTCCTCCGCCTGTgc  <  2:13399/251‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                           AAAAGTGGCGCGCGCAGTAGAAGAAGGGGCGAGAGTGGCGTTCGGTGGCAAAGCGGTAGAGGGGAAAGGATATTATTATCCGCCGACATTGCTGCTGGATGTTCGCCAGGAAATGTCGATTATGCATGAGGAAACCTTTGGCCCGGTGCTGCCAGTTGTCGCATTTGACACGCTGGAAGATGCTATCTCAATGGCTAATGACAGTGATTACGGCCTGACCTCATCAATCTATACCCAAAATCTGAACGTCGCGATGAAAGCCATTAAAGGGCTGAAGTTTGGTGAAACTTACATCAACCGTGAAAACTTCGAAGCTATGCAAGGCTTCCACGCCGGATGGCGTAAATCCGGTATTGGCGGCGCAGATGGTAAACATGGCTTGCATGAATATCTGCAGACCCAGGTGGTTTATTTACAGTCTTAATGAGTGAAAGAGGCGGAGGTTTTTTCCTCCGCCTGTGC  >  NC_000913/1489248‑1489709

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑