Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,308,684	T→C	intergenic (‑39/‑104)	clsA ← / → yciY	cardiolipin synthase A/uncharacterized protein YciY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,308,684	0	T	C	100.0%	61.9 / NA	20	intergenic (‑39/‑104)	clsA/yciY	cardiolipin synthase A/uncharacterized protein YciY
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/7);  total (13/7)

GGCGTTTGCCTAAATGGAGCTCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGGAGGGAACTGCGCGTCGTTTCATTAGAATGCGTAAAGTTACGCCTGCAATGAGCAACCAGTATCCCAGAATGGCCAACCAACTCACCAACGTATAAACGGTTGTCATAGATTAGAAATCCTTTTGAAAGCGCATTGTTATGAGTTTACGCATATGGATTCATCTGGCAAATAAAAACGCGGGAAAAGCACTGGTCAGCGTCGGGGGTGGGGGTATAATGACCATTCTGTTATTGCATAGAGTAGTTAACATGAAGCGGAGTAGAACGGAAGTGGGGCGCTGGCGCATGCAGCGTCAGGCTAGCCGACGTAAATCGCGTTGGCTTGAGGGGCAATCGCGCCGAAATATGCGTATCCACAGCATCAGGAAGTGCATTCTAAACAAAC  >  NC_000913/1308447‑1308923                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                ggcgTTTGCCTAAATGGAGCTCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGGAGGGAACTGCGCGTCGTTTCATTAGAATGCGTAAAGTTACGCCTGCAATGAGCAACCAGTATCCCAGAATGGCCAACCAACTCACCAACGTATAAACGGTTGTCATAGATTAGAAATCCTTTTGAAAGCGCATTGTTATGAGTTCACGCATATGGAtt                                                                                                                                                                                                                                    <  1:96298/251‑1 (MQ=255)        gCCTAAATGGAGCTCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGGAGGGAACTGCGCGTCGTTTCATTAGAATGCGTAAAGTTACGCCTGCAATGAGCAACCAGTATCCCAGAATGGCCAACCAACTCACCAACGTATAAACGGTTGTCATAGATTAGAAATCCTTTTGAAAGCGCATTGTTATGAGTTCACGCATATGGATTCATCTg                                                                                                                                                                                                                              <  2:293133/250‑1 (MQ=255)                              gCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGGAGGGAACTGCGCGTCGTTTCATTAGAATGCGTAAAGTTACGCCTGCAATGAGCAACCAGTATCCCAGAATGGCCAACCAACTCACCAACGTATAAACGGTTGTCATAGATTAGAAATCCTTTTGAAAGCGCATTGTTATGAGTTCACGCATATGGATTCATCTGGCAAATAAAAACGCGGGaaa                                                                                                                                                                                                          >  1:229420/1‑248 (MQ=255)                                     aGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGGAGGGAACTGCGCGTCGTTTCATTAGAATGCGTAAAGATACGCCTGCAATGAGCAACCAGTATCCCAGAATGGCCAACCAACTCACCAACGTATAAACGGTTGTCATAGATTAGAAATCCTTTTGCAAGCGCATTGTTATGAGTTCACGCATATGGATTCATCTGGCACATAAAAACGCGGGAAAAGCACTGGTc                                                                                                                                                                                                >  2:5806/1‑251 (MQ=255)                                                  gACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGGAGGGAACTGCGCGTCGTTTCATTAGAATGCGTAAAGTTACGCCTGCAATGAGCAACCAGTATCCCAGAATGGCCAACCAACTCACCAACGTATAAACGGTTGTCATAGATTAGAAATCCTTTTGAAAGCGCATTGTTATGAGTTCACGCATATGGATTCATCTGGCAAATAAAAACGCGGGAAAAGCACTGGTCAGCGTCgggggtg                                                                                                                                                                                   <  2:2770/251‑1 (MQ=255)                                                                tGTAACTAATCAACAGCCAGGACATCGCGGCGGGAACTGCGCGTCATTTCATTAAAATGCGTAAAGTTACGCCTGCACTGAGCAACCAGTATCCCAGAATGGCCAACCAACTCACCAACGTATAAACGGTTGTCATAGATTAGAAATCCTTTTGAAAGCGCATTGTTATGAGTTCACGCATATGGATTCATCTGGCAAATAAAAACGCGGGAAAAGCACTGGTCAGCGTCGGGGGTGGGGGTATAATGAcc                                                                                                                                                                     >  2:140810/1‑251 (MQ=255)                                                                          cAACAGCCAGGCCATCGCGGAGGGAACTGCGCGTCGTTTAATTAGAATGCGTAAAGTTACGCCTGCAATGAGCAACCAGTATCCCAGAATGGCCAACCAACTCACCAACGTATAAACGGTTGTCATAGATTAGAAATCCTTTTGAAAGCGCATTGTTATGAGTTCACGCATATGGATTCATCTGGCAAATAAAAACGCGGGAAAAGCACTGGTCAGCGTCGGGGGTGGGGGTATAATGACCATTCTGTTAt                                                                                                                                                           <  1:7803/251‑1 (MQ=255)                                                                                                   aCTGCGCGTCGTTTCATTAGAATGCGTAAAGTTACGCCTGCAATGAGCAACCAGTATCCCAGAATGGCCAACCAACTCACCAACGTATAAACGGTTGTCATAGATTAGAAATCCTTTTGAAAGCGCATTGTTATGAGTTCACGCATATGGATTCATCTGGCAAATAAAAACGCGGGAAAAGCACTGGTCAGCGTCGGGGGTGGGGGTATAATGACCATTCTGTTATTGCATAGAGTAGTTAACATGAAGCg                                                                                                                                  >  2:62502/1‑251 (MQ=255)                                                                                                         cgtcgtTTCACTAGAATGAGCCAAGCTACGCCTGCAATGACCAACCAGTCTCCCAGAATGGACAAACAGCTCACCAACGTCTCACAGGTTGTTCTAGAATAGAACTCATTTTGCAAGCGAATTGTTATGAGTTCACGCATCTGGATTCATCTGGCAAATAAAAACGCGGGCAAAGCACTGGTCAGCGTCGGGGGAGGGGGTATAATGACCATTCCGTTATTGCATAGAGTAGTTAACATGAAGCgggga                                                                                                                              >  2:155529/1‑246 (MQ=255)                                                                                                          gtcgtTTCATTAGAATGCGTAAAGTTACGCCTGCAATGAGCAACCAGTATCCCAGAATGGCCAACCAACTAACAAACGTATACACGGATGTCATAGATTAGAAATCCTTTTGAAAGCGCATTGTTATGAGTTCACGCATATGGATTCATCTGGCAAATAAAAACGCGGGAAAAGCCCTGGTCAGCGTCGGGGGTGGGGGTATAATGACCATTCTGTTATTGCATAGAGTAGTTAAAATGAAGCGGAGt                                                                                                                              >  1:211537/1‑248 (MQ=255)                                                                                                            cgtTTCATTAGAATGCGTAAAGTTACGCCTGCAATGAGCAACCAGTATCCCAGAATGGCCAACCAACTCACCAACGTATAAACGGTTGTCATAGATTAGAAATCCTTTTGAAAGCGCATTGTTATGAGTTCACGCATATGGATTCATCTGGCAAATAAAAACGCGGGAAAAGCACTGGTCAGCGTCGGGGGTGGGGGTATAATGACCATTCTGTTATTGCATAGAGTAGTTAACATGAAGCGGAGTAGAAc                                                                                                                         >  1:200646/1‑251 (MQ=255)                                                                                                                                              tGAGCAACCAGTCTCCCAGACTGGCCAACCAACTCACCAACGTATAAACGGTTGTCATAGATTAGAAATCCTTTTGAAAGAGCATTGTTATGAGTTCACGCATATGGATTCATCTGGCAAATAAAAACGCGGGAAAAGCACTGGTCAGCGACGGGGGTGGGGGTATAATGACCATTCTGTTATTGCATAGAGTAGTTAACATGAAGCGGAGTAGAACGGAAGTGGGGCGCTGGCGCATGCAGCGTCAGGCt                                                                                       >  1:122943/1‑251 (MQ=255)                                                                                                                                              tGAGCAACCAGTATCCCAGAATGGCCAACCAACTCACCAACGTATAAACGGTTGTCATAGATTAGAAATCCTTTTGAAAGCGCATTGTTATGAGTTCACGCATATGGATTCATCTGGCAAATAAAAACGCGGGAAAAGCACTGGTCAGCGTCGGGGGTGGGGGTATAATGACCATTCTGTTATTGCATAGAGTAGTTAACATGAAGCGGAGTAGAACGGAAGTGGGGCGCTGGCGCATGCAGCGTCAGGCt                                                                                       >  2:60375/1‑251 (MQ=255)                                                                                                                                                              cAGAATGGCCAACCAACTCACCAACGTATAAACGGTTGTCATAGATTAGAAATCCTTTTGAAAGCGCATTGTTATGAGTTCACGCATATGGATTCATCTGGCAAATAAAAACGCGGGAAAAGCACTGGTCAGCGTCGGGGGTGGGGGTATAATGACCATTCTGTTATTGCATAGAGTAGTTAACATGAAGCGGAGTAGAACGGAAGTGGGGCGCTGGCGCATGCAGCGTCAGGCTAGCCGACGTAAATcgc                                                                       >  1:58552/1‑251 (MQ=255)                                                                                                                                                                                             aCGGTTGTCATAGATTAGAAATCCTTTTGAAAGCGCATTGTTATGAGTTCACGCATATGGATTCATCTGGCAAATAAAAACGCGGGAAAAGCACTGGTCAGCGTCGGGGGTGGGGGTATAATGACCATTCTGTTATTGCATAGAGTAGTTAACATGAAGCGGAGTAGAACGGAAGTGGGGCGCTGGCGCATGCAGCGTCAGGCTAGCCGACGTAAATCGCGTTGGCtt                                                               >  2:237910/1‑228 (MQ=255)                                                                                                                                                                                             aCGGTTGTCATAGATTAGAAATCCTTTTGAAAGCGCATTGTTAGGAGTTCACGCATATGGATTCATCTGGCAAATAAAAACGCGGGAAAAGCACTGGTCAGCGTCGGGGGTGGGGGTATAATGACCATTCTGTTATTGCATAGAGTAGTTAACATGAAGCGGAGTAGAACGGAAGTGGGGCGCTGGCGCATGCAGCGTCAGGCTAGCCGACGTAAATCGCGTTGGCtt                                                               <  1:237910/228‑1 (MQ=255)                                                                                                                                                                                                        aGATTAGAAATCCTTTTGAAAACGCATTGTTATGAGTTCACGCATATGGATTCATCTGGCAAATAAAAACGCGGGAAAAGCACTGGTCAGCGTCGGGGGTGGGGGTATAATGACCATTCTGTTATTGCATAGAGTAGTTAACATGAAGCGGAGTAGAACGGAAGTGGGGCGCTGGCGCATGCAGCGTCAGGCTAGCCGACGTAAATCGCGTTGGCTTGAGGGGCAATCGCGCCGAAATATGCGTATCcaca                             <  2:22566/251‑1 (MQ=255)                                                                                                                                                                                                                   tcTTTTGAAAGCGCATTGTTATGAGTTCACGCATATGGATTCATCTGGCAAATAAAAACGCGGGAAAAGCACTGGTCAGCGTCGGGGGTGGGGGTATAATGACCATTCTGTTATTGCATAGAGTAGTTAACATGAAGCGGAGTAGAACGGAAGTGGGGCGCTGGCGCATGCAGCGTCAGGCTAGCCGACGTAAATCGCGTTGGCTTGAGGGGCAATCGCGCCGAAATATGCGTATCCACAGCATCAGGaa                   <  2:300/249‑1 (MQ=255)                                                                                                                                                                                                                    cTTTTGAAAGCGCATTGTTATGAGTTCACGCATATGGATTCATCTGGCAAATAAAAACGCGGGAAAAGCACTGGTCAGCGTCGGGGGTGGGGGAATAATGACCATTCTGTTATTGCATAGAGTAGTTAACATGAAGCGGAGTAGAACGGAAGTGGGGCGCTGGCGCATGCAGCGTCAGGCTAGCCGACGTAAATCGCGTTGGCTTGCGGGGAAATCGCGCCGAACTATGCGTATCCACAGCATCAGGAAgg                 >  2:59154/1‑250 (MQ=255)                                                                                                                                                                                                                                     ttATGAGTTCACGCATATGGATTCATCTGGCAAATAAAAACGCGGGAAAAGCACTGGTCAGCGTCGGGGGTGGGGGTATAATGACCATTCTGTTATTGCATAGAGTAGTTAACATGAAGCGGAGTAGAACGGAAGTGGGGCGCTGGCGCATGCAGCGTCAGGCTAGCCGACGTAAATCGCGTTGGCTTGAGGGGCAATCGCGCCGAAATATGCGTATCCACAGCATCAGGAAGTGCATTCTaaacaaac  >  1:72547/1‑249 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                GGCGTTTGCCTAAATGGAGCTCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGGAGGGAACTGCGCGTCGTTTCATTAGAATGCGTAAAGTTACGCCTGCAATGAGCAACCAGTATCCCAGAATGGCCAACCAACTCACCAACGTATAAACGGTTGTCATAGATTAGAAATCCTTTTGAAAGCGCATTGTTATGAGTTTACGCATATGGATTCATCTGGCAAATAAAAACGCGGGAAAAGCACTGGTCAGCGTCGGGGGTGGGGGTATAATGACCATTCTGTTATTGCATAGAGTAGTTAACATGAAGCGGAGTAGAACGGAAGTGGGGCGCTGGCGCATGCAGCGTCAGGCTAGCCGACGTAAATCGCGTTGGCTTGAGGGGCAATCGCGCCGAAATATGCGTATCCACAGCATCAGGAAGTGCATTCTAAACAAAC  >  NC_000913/1308447‑1308923

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑