Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	421,027	C→T	R14R (CGC→CGT)	proY →	putative transporter ProY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	421,027	0	C	T	97.1%	106.0 / NA	35	R14R (CGC→CGT)	proY	putative transporter ProY
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (15/19);  minor base G (0/1);  total (15/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

CTGGGCGCAGCGTTTACCGCTGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTATGG‑CACTGGGTTCAGCAATTGGCACCGGGCTGTTTTACGGTTCGGCAGACGCCATCAAAATGGCCGGTCCGAGCGTGTTGTTGGCCTATATTATCGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTACCGGCTGGACC  >  NC_000913/420784‑421267                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                  cTGGGCGCAGCGTTTACCGCTGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGCCCTCCAGCGCTCAGTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGCATTGATGGCAAGTAAGAACAAGCTAAAGCGTGGGATAAGAACCCGTCACATAc                                                                                                                                                                                                                                            >  2:247739/1‑251 (MQ=255)  tGGGCGCAGCGTTTACCGCTGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGTCACATACg                                                                                                                                                                                                                                           >  1:327051/1‑251 (MQ=255)    ggCGCAGCGTTTACCGCTGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGTCACATACGCt                                                                                                                                                                                                                                         <  1:30572/251‑1 (MQ=255)        cAGCGTTTACCGCTGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTGCCCGTCACTTACGCTTTAt                                                                                                                                                                                                                                     <  2:214024/251‑1 (MQ=255)                      gggtgAACAAGGTCTGGCGTGGATAATGCCATCAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGTCTAAGTACCCGGCACATTCGCTTTATGG‑CACTTGGTTCAg                                                                                                                                                                                                                      <  2:36473/247‑1 (MQ=255)                        ccgtaCAAGGTCTGTCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCTCTGAACATTTGTTTTAACCACGGGGGTGCGATGCCCCGTGGTTTTTTATTGTGGTGATGGGTTAGGAATTGATGGAAAGTAAGAACATGCTAAAGCGTGGGCGAAGTTCCCGTCTCATACGCTTTATGG‑CACTGGGTTCAGCa                                                                                                                                                                                                                    <  2:197036/247‑1 (MQ=255)                              aaGGTCTGGCGTGGTTCATGCCAACAGTGGTGATGGTGATTCTGGCCATTATCTGGGATCGCGAGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGTGACATACGCTTTATGG‑CACTGGGTTCAGCAATTGGc                                                                                                                                                                                                              >  2:33647/1‑251 (MQ=255)                                   cTGGCGTGGTTAATGCCAACAGTGGAGATGGTGGTTCTGGCCATTCTCTGGGATCGCGCGGCAGGACGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAACGCGTGGGCTAAGTACCCGTCACATACACTTTATGG‑CACTGGGTTCAGCAATTGGCACCgg                                                                                                                                                                                                         >  1:302571/1‑251 (MQ=255)                                                tGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGTCACATACGCTTTATGG‑CACTGGGTTCAGCAATTGGCACCGGGCTg                                                                                                                                                                                                     <  1:83179/242‑1 (MQ=255)                                                tGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGTCACATACGCTTTATGG‑CACTGGGTTCAGCAATTGGCACCGGGCTg                                                                                                                                                                                                     >  2:83179/1‑242 (MQ=255)                                                          ggtgATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGTCACATACGCTTTATGG‑CACTGGGTTCAGCAATTGGCACCGGGCTGTTTTACGGTTCGGCAGAc                                                                                                                                                                                   <  2:2417/250‑1 (MQ=255)                                                            tgATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGTCACATACGCTTTATGG‑CACTGGGTTCAGCAATTGGCACCGGGCTGTTTTACGGTTCGGCAGACGcc                                                                                                                                                                                >  1:50757/1‑251 (MQ=255)                                                                ggtggtTCTGGCCATTATCGGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGGTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTTCCCGTCACATACGCTTTATGG‑CACTGGGTTCAGCAATTGGCACCGGGCTGTTTTACGGTTCGGCAGACGCCATCa                                                                                                                                                                            <  2:292453/251‑1 (MQ=255)                                                                         ggCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGTCACATACGCTTTATGG‑CACTGGGTTCAGCAATTGGCACCGGGCTGTTTTACGGTTCGGCAGACGCCATCAAAATGGcct                                                                                                                                                                   <  2:47249/251‑2 (MQ=255)                                                                               tATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGTCACATACGCTTTATGG‑CACTGGGTTCAGCAATTGGCACCGGGCTGTTTTACGGTTCGGCAGACGCCATCAAAATGGCCGGTCCg                                                                                                                                                              >  1:111831/1‑250 (MQ=255)                                                                                    gggATCGCGCGGCAGGTCGTCAGGGGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGTCACATACGCTTTATGG‑CACTGGGTTCAGCAATTGGCACCGGGCTGTTTTACGGTTCGGCAGACGCCATCAAAATGGCCGGTCCGAGCg                                                                                                                                                          <  1:247739/249‑1 (MQ=255)                                                                                                 aGGTCGTCAGGTGCCCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAAGTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGTCACATACGCTTTATGG‑CACTGGGTTCAGCAATTGGCACCGGGCTGTTTTACGGTTCGGCAGACGCCATCAAAATGGCCGGTCCGAGCGTGTTGTTGGCCtata                                                                                                                                           <  1:276590/251‑1 (MQ=255)                                                                                                           gTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGTCACATACGCTTTATGG‑CACTGGGTTCAGCAATTGGCACCGGGCTGTTTTACGGTTCGGCAGACGCCATCAAAATGGCCGGTCCGAGCGTGTTGTTGGCCTATATTATCggtgg                                                                                                                                 <  1:45941/251‑1 (MQ=255)                                                                                                              aCCTCTAGCGCTCACTAACTCACTGAATATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGCTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTCCCCTTCACATACGCTTTATGG‑CACTGGGTTCAGCAATGGGCACCGGGCTGTTTTACGGTTCGGCAGACGCCATCAAAATGGCCGGTCCGAGCGTGTTGTTGGCCTATCTTATCTGGGGTAt                                                                                                                              >  2:174300/1‑251 (MQ=255)                                                                                                                 tCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGTCACATACGCTTTATGG‑CACTGGGTTCAGCAATTGGCACCGGGCTGTTTTACGGTTCGGCAGACGCCATCAAAATGGCCGGTCCGAGCGTGTTGTTGGCCTATATTATCGGTGGTATcgc                                                                                                                           >  2:148418/1‑251 (MQ=255)                                                                                                                    aGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGTCACATACGCTTTATGG‑CACTGGGTTCAGCAATTGGCACCGGGCTGTTTTACGGTTCGGCAGACGCCATCAAAATGGCCGGTCCGAGCGTGTTGTTGGCCTATATTATCGGTGGTATCgcggc                                                                                                                        <  1:320605/251‑1 (MQ=255)                                                                                                                                                   aaCCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGTCACATACGCTTTATGG‑CACTGGGTTCAGCAATTGGCACCGGGCTGTTTTACGGTTCGGCAGACGCCATCAAAATGGCCGGTCCGAGCGTGTTGTTGGCCTATATTATCGGTGGTATCgcggc                                                                                                                        <  2:286688/220‑1 (MQ=255)                                                                                                                                                   aaCCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGTCACATACGCTTTATGG‑CACTGGGTTCAGCAATTGGCACCGGGCTGTTTTACGGTTCGGCAGACGCCATCAAAATGGCCGGTCCGAGCGTGTTGTTGGCCTATATTATCGGTGGTATCgcggc                                                                                                                        >  1:286688/1‑220 (MQ=255)                                                                                                                                                   aaCCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGTCACATACGCTTTATGG‑CACTGGGTTCAGCAATTGGCACCGGGCTGTTTTACGGTTCGGCAGACGCCATCAAAATGGCCGGTCCGAGCGTGTTGTTGGCCTATATTATCGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATg                                                                                         >  1:307974/1‑251 (MQ=255)                                                                                                                                                               tcGATTCCCCGTTGTTTTTTATTGGGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGTCACATACGCTTTATGG‑CACTGGGTTCAGCAATTGGCACCGGGCTGTTTTACGTTTCGGCAGACGCCATCAAAATGGCCGGTCCGAGCGTGTTGTTGGCCGATATTATCGGTGGTAGCGCGGCGTATATCATGATGCGTGCGCTGGGGGAAATTTCGGTACATa                                                                               <  2:209728/248‑1 (MQ=255)                                                                                                                                                               gCGATGTCGCGTTGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTCCCCGTCTGATACGCTTTATGG‑CGCTGGGTTCAGCAATTGGCACCGGGCTGTTTTACGGTTCGGCAGACGCCATCAAAATGGCCGGTCCGAGCGTGTTGTTGGCCTATATTATCGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATaa                                                                              <  1:308415/250‑1 (MQ=255)                                                                                                                                                                       ccGTGGTTTTTTATTGTGTTGATGGGCTAGGAATTGATGGAAAGTAAGACCAAGCTAAAGCGTGGGCTAAGTACCCGTCACATACGCTTTATGG‑CACTGGGTTCAGCAATTGGCACCGGGCTGTTTTACGGTTCGGCAGACGCCATCAAAATGGCCGGTCCGAGCGTGTTGTTGGCCTATATTATCGGTGGTATCGCGGCGTATATCATTCTGCGTGCGCTGGGGGAAATGCCGGTACATAACTCGgccgc                                                                     >  2:292011/1‑251 (MQ=255)                                                                                                                                                                                                 ttAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGTCACATACGCTTTATGG‑CACTGGGTTCAGCAATTGGCACCGGGCTGTTTTACGGTTCGGCAGACGCCATCAAAATGGCCGGTCCGAGCGTGTTGTTGGCCTATATTATCGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAgg                                           >  1:47739/1‑251 (MQ=255)                                                                                                                                                                                                                aaGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGTCACATACGCTTTATGG‑CACTGGGTTCAGCAATTGGCACCGGGCTGTTTTACGGTTCGGCAGACGCCATCAAAATGGCCGGTCCGAGCGTGTTGTTGGCCTATATTATCGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGc                            <  1:148418/251‑1 (MQ=255)                                                                                                                                                                                                                      gAACAAGCTAAAGCGTGGGCTAAGTACCCGTCACATACGCTTTATGG‑CACTGGGTTCAGCAATTGGCACCGGGCTGTTTTACGGTTCGGCAGACGCCATCAAAATGGCCGGTCCGAGCGTGTTGTTGGCCTATATTATCGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGc                        >  1:317798/1‑249 (MQ=255)                                                                                                                                                                                                                             cgaaggCGTGGGCTAAGTACCCGTCACATACGTTTTATGG‑CACTGGGTTCAGCAATTGGCACCGGGCTGGTTTACGGTTCGGCAGACGCCATCAAAATGGCCGGTCCGAGCGTGTTGTTGGCCTATATTATCGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCGCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACAt               <  2:272122/246‑1 (MQ=255)                                                                                                                                                                                                                                   cGTGGGCCAAGTACCCGTCCCCTTCGCTTTATGG‑CCCTGGGTTCAGCAATTGGGCCCGGGCTTTTTTACCGTTCGGCAGACGCCATCAAAATGGCCGGTCCGAGCGTGTTGTTGGCCTATATTATCGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTACCgg         <  2:111831/251‑1 (MQ=255)                                                                                                                                                                                                                                      gggCTAAGTACCCGTCACAGGCGCTTTATGG‑CACTGGGTTCAGCAATTGGCACCGGGCTGTTTTACGGTTCGGCAGACGCCATCAAAATGGCCGGTCCGAGCTTGTTGTTGGCCTATATTATCGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGTAAATGTCGGTACAGAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTACCGGCt       <  1:216007/250‑1 (MQ=255)                                                                                                                                                                                                                                       ggCTAAGTACCCGTCGCATCCGCTTTATGGTCA‑TGGGTTCAGCACTTGGCACCGGGCTGCTTTCCGGTTCGGCACACGCCATCAAAATGGCCGGTCCGTGCGTGTTGTTGGCCTACATTATCGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGGCAATGTAGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTCTGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTAcaggatgg     >  2:127945/1‑244 (MQ=255)                                                                                                                                                                                                                                           aaGTGCCCGTCACATACGCTTTATGG‑CACTGGGTTCAGCAATTGGCACCGGGCTGTTTTACGGTTCGGCAGACGCCATCAAAATGGCCGGTCCGAGCGTGTTGTTGGCCTATATTATCGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTACCGGCTGGAcc  <  1:173716/250‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                  CTGGGCGCAGCGTTTACCGCTGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTATGG‑CACTGGGTTCAGCAATTGGCACCGGGCTGTTTTACGGTTCGGCAGACGCCATCAAAATGGCCGGTCCGAGCGTGTTGTTGGCCTATATTATCGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTACCGGCTGGACC  >  NC_000913/420784‑421267

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑