Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,962,172	C→T	A67A (GCC→GCT)	yecT →	protein YecT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,962,172	0	C	T	100.0%	53.7 / NA	16	A67A (GCC→GCT)	yecT	protein YecT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/9);  total (7/9)

CGATAACGGTTGAATTTAAGGAATACCGCAGTGTTTAAATTTCTTGTATTAACATTAGGCATTATCTCTTGCCAGGCTTACGCAGAAGATACGGTTATAGTAAACGACCATGACATTTCAGCCATCAAAGATTGTTGGCAAAAAAATTCAGATGATGATACTGACGTTAACGTGATCAAATCATGCCTGCGACAAGAATACAATCTCGTCGATGCGCAATTAAATAAAGCCTATGGTGAAGCTTATCGTTATATAGAACAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTGGATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTTCAGGATTTAAGTGACCCTTATTCTGAATCAGAATGGCTCTCATGTATGATCATACAGACCAATACGCGAACTCGCCAGTTGCAGCTAT  >  NC_000913/1961942‑1962413                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                  cGATAACGGTTGAATTTAAGGAATAACGCAGTGTTTAAATTTCTTGTATTAACATTAGGCATTATCTCTTGCCAGGCTTACGCAGAAGATACGGTTATAGTAAACGACCATGACATTTCAGCCATCAAAGATTGTTGGCAAAAAAATTCAGATGATGATACTGACGTTAACGTGATCAAATCATGCCTGCGACAAGAATACAATCTCGTCGATGCGCAATTAAATAAAGCTTATGGTGAAGCTTATCGTta                                                                                                                                                                                                                               <  1:105264/251‑1 (MQ=255)  gATAAAGGTTGAATTTAAGGAATACAGCAGTGTTTAAATTTCTAGTATTAACATTAGGCATTATCTCTTGCCAGGCTTACGCAGAAGATACGGTTATAGTAAACGACCATGACATTTCAGACATCAAAGATTGTTGGCAAAAAAATTCAGATGATGATACTGACGTTAACGTGATCAAATCATGCTTGCAAAAAGAATACAATCTCGTCGATGGGCAATTAAATAAAGCTTATGGTGAAGCTTATCGTtat                                                                                                                                                                                                                              >  2:317492/1‑251 (MQ=255)                          ccGCAGTGTTTAAATTTCTTGTATTAACATTAGGCATTATCTCTTGCCAGGCTTACGCAGAAGATACGGTTATAGTAAACGACCATGACATTTCAGCCATCAAAGATTGTTGGCAAAAAAATTCAGATGATGATACTGACGTTAACGTGATCAAATCATGCCTGCGACAAGAATACAATCTCGTCGATGCGCAATTAAATAAAGCTTATGGTGAAGCTTATCGTTATATAGAACAAGTGCCACGCACAGgt                                                                                                                                                                                                      <  1:228315/251‑1 (MQ=255)                                         ttCTTGTATTAACATTAGGCATTATCTCTTGCCAGGCTTACGCAGAAGATACGGGTATAGTAAACGACCATTACATTTCAGCCATCAAAGATTGTTGGCAAAAAAATTCAGATGAGGATACTGACGTTAACGTGAGCAAATCATGCCTGCGACAAGAATACAATCGCGTCGATGCGCAATTAAATAAAGCTTATGGTGTAGCGTATCGTTATATAGAACAAGTGCCACGCACAGGTGTAAAAAAACCTGa                                                                                                                                                                                        <  1:40303/250‑1 (MQ=255)                                             tGTATTAACATTAGGCATTATCTCTTGCCAGGCTTGCGCAGAATATACGGTGATAGTAAACGACCATGACATTTCAGCCATCAAAGATTGTTGGCAAAAAAAGTCAGATGATGATACTGACGTTAGCATGATCAAATCATGCCTGCGAAAAGAATACAATCTCGGCGACCCGCAATTAAATAAAGCTTATGGTGAAGCTTATCGTTATATAGAACAAGGGCCCCGCACAGGTGTAAAAAAACCTGATAct                                                                                                                                                                                    <  2:130761/250‑2 (MQ=255)                                                         aGGCATTATCTCTTGCCAGGCTTACGCAGAAGATACGGTTATAGTAAACGACCATGACATTTCAGCCATCAAAGATTGTTGGCAAAAAAATTCAGATGATGATACTGACGTTAACGTGATCAAATCATGCCTGCGACAAGAATACAATCTCGTCGATGCGCAATTAAATAAAGCTTATGGTGAAGCTTATCGTTATATAGAACAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCGAACaacttaac                                                                                                                                                                        <  2:125034/250‑1 (MQ=255)                                                                                  gCAGAAGATACGGTTATAGTAAACGACCATGACATTTCAGCCATCAAAGATTGTTGGCAAAAAAATTCAGATGATGATACTGACGTTAACGTGATCAAATCATGCCTGCGACAAGAATACAATCTCGTCGATGCGCAATTAAATAAAGCTTATGGTGAAGCTTATCGTTATATAGAACAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCctg                                                                                                                                              <  1:7298/251‑1 (MQ=255)                                                                                     gaagaTACGGTTATAGTAAACGACCATGACATTTCAGCCATCAAAGATTGTTGGCAAAAAAATTCAGATGATGATACTGACGTTAACGTGATCAAATCATGCCTGCGACAAGAATACAATCTCGTCGATGCGCAATTAAATAAAGCTTATGGTGAAGCTTATCTTTATATAGAACAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCctggct                                                                                                                                           <  1:31781/251‑1 (MQ=255)                                                                                       agaTACGGTTATAGTAAACTACCATGACATTTCAGCCATCTAAGATTGTTGGCAAAAAAATTCAGATGATGATACTGACGTTAACGTGATCAAATCATGCCTGCGACAAGAATACAATCTCGTCGATGCGCAATTAAATAAAGCTTATGGTGAAGCTTATCGTTATATAGAACAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTACAAAATCACAGCGAGCctggctgg                                                                                                                                         >  1:143727/1‑251 (MQ=255)                                                                                                      aaaCGACCATGACATTTCAGCCATCAAAGATTGTTGGCAAAAACATTCAGATGATGATACTGACGTTAACGTGCTCAAATCATGCATGCGACAAGAATACAATCTCGTCGATGCGCAATTAATTAAAGCTTATGGTGAAGCTTATCGTTATATAGAACAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTGGATTTTAGGGACaa                                                                                                                            >  2:101411/1‑249 (MQ=255)                                                                                                                                   aTTGTTGGCAAAAAAATTCAGATGATGATACTGACGTTAACGTGATCAAATCATGCCTGCGACAAGAATACAATCTCGTCGATGCGCAATTAAATAAAGCTTATGGTGAAGCTTATCGTTATATAGAACAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTGGATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGAc                                                                                             <  1:10806/251‑1 (MQ=255)                                                                                                                                                          gatgatACTGAGGTTAATGTGATAAAATAATGCCTGCGACAAGAATAAAATCTCGTCGATGCGCAATTAAATAACGCTTATGGAGCAGCTTATCGTTATATAGAACAAGTGACACGCACAGGTGTAAAACAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTGGATTTTAGGGACAAAGAATGTGAATTAATCCTTTAAAATGAGGCCGTTCAGGATTTAAGTGACCCtt                                                                       >  2:44362/1‑250 (MQ=255)                                                                                                                                                                                       atgcctccgcaaaGAATACAATCTCGTCGATGCGCAATTAAATAAAGCTTATGGAGAAGCTTATCGTTATATAGAACAAGTGCAACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTGGATTTTAAGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTTCAGGATTTAAGTGACCCTTATTCTGAATCAGAATGGCTCTCATGTATg                                          >  2:293010/12‑250 (MQ=255)                                                                                                                                                                                                                          aaataaataaaGCTTATGGTGAAGCTTATCGTTATATAGAACAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTAACTAGCTTAAAAAATCACAGCGAGCCTGGCTGGAGTTTAGGTACAAAGAATTTGAATTAATGCTTTCAAATGAGGACGTTCGGGATTTAAGGGTCCTTTATTCTGAATCAGAATGGCTCTCATGTATGATCATACAGACCAATACGCGAACTCGCCAGTTGCAg      <  1:36981/248‑1 (MQ=255)                                                                                                                                                                                                                            ttAAATAAAGCTTATGGTGAAGCTTATCGTTATATAGAACAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTGGATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTTCAGGATTTAAGTGACCCTTATTCTGAATCAGAATGGCTCTCATGTATGATCATACAGACCAATACGCGAACTCGCCAGTTGCAGCt    >  2:238874/1‑251 (MQ=255)                                                                                                                                                                                                                               aataaaGCTTATGGTGAAGCTTATCGTTATATAGAACAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTGGATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTTCAGGATTTAAGTGACCCTTATTCTGAATCAGAATGGCTCTCATGTATGATCATACAGACCAATACGCGAACTCGCCAGTTGCAGCtat  >  1:31284/1‑250 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                  CGATAACGGTTGAATTTAAGGAATACCGCAGTGTTTAAATTTCTTGTATTAACATTAGGCATTATCTCTTGCCAGGCTTACGCAGAAGATACGGTTATAGTAAACGACCATGACATTTCAGCCATCAAAGATTGTTGGCAAAAAAATTCAGATGATGATACTGACGTTAACGTGATCAAATCATGCCTGCGACAAGAATACAATCTCGTCGATGCGCAATTAAATAAAGCCTATGGTGAAGCTTATCGTTATATAGAACAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTGGATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTTCAGGATTTAAGTGACCCTTATTCTGAATCAGAATGGCTCTCATGTATGATCATACAGACCAATACGCGAACTCGCCAGTTGCAGCTAT  >  NC_000913/1961942‑1962413

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑