Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,511,466	C→T	V293V (GTG→GTA)	yhfZ ←	putative DNA‑binding transcriptional regulator YhfZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,511,466	0	C	T	100.0%	150.6 / NA	49	V293V (GTG→GTA)	yhfZ	putative DNA‑binding transcriptional regulator YhfZ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/34);  total (15/34)

CGTTATCCAGCGGCTCTATTTCTTCACCACGGCGACTGCGCATCAGTGCACTCGCCATATGTGTCATCGCCATCGTTCCTTGCTCACTGCGCACCGGCAGATGGCACTCTGTTTCCAGTACGTTGACGACCTGCATCATGCCTTTGCAGATGTCCTTATCAATAACGCCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTCACTACCCGTTGTT‑GATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAG  >  NC_000913/3511221‑3511711                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                       cGTTATCCAGCGGCTCTATTTCTTCACCACGGCGACTGCGCATCAGTGCACTCGCCATATGTGTCATCGCCATCGTTCCTTGCTCACTGCGCACCGGCAGATGGCACTCTGTTTCCAGTACGTTGACGACCTGCATCATGCCTTTGCAGATGTCCTTATCAATAACGCCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTtactac                                                                                                                                                                                                                                                   >  1:270054/1‑251 (MQ=255)                  aTTTCTTCACCACGGCGACTGCGCATCAGTGCACTCGCCATATGTGTCATCGCCATCGTTCCTTGCTCACTGCGCACCGGCAGATGGCACTCTGTTTCCAGTACGTTGACGACCTGCATCATGCCTTTGCAGATGTCCTTATCAATAACGCCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTTACTACCCGTTGTT‑GATGGGCg                                                                                                                                                                                                                                  >  1:71018/1‑250 (MQ=255)                         caccacGGCGACTGCGCATCAGTGCACTCTCCATATGTGTCATCGCCATCGTTCCTTGCTTACTGGGCAACAGCAGATGGCACTCTTTTTCCATTACGTTGACGACCTGCATCATGCCTTTGCAGATGTTCTTATTAACGACGCCTGCCTTGCAAAGCAGGTTGAGCCTGCTTTCCATATCAGATGCCTTTCGTTTAAGAACTCGGTGCCTGTTCCCCTCTTACTACCCGGTGTG‑GATGGGCGAGTAGGGc                                                                                                                                                                                                                          <  1:239182/251‑1 (MQ=255)                           acacGGCGACTGCGCATCAGTGCACTCGCCATATGTGTCATCGCCATCGTTCCTTGCTCACTGCGCACCGGCAGATGGCACTCTGTTTCCAGTACGTTGACGACCTGCATCATGCCTTTGCAGATGTCCTTATCAATAACGCCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTTACTACCCGTTGTT‑GATGGGCGAGCAGGGc                                                                                                                                                                                                                          <  1:316726/248‑1 (MQ=255)                                         cATCAGTGCACTCGCCATATGTGTCATCGCCATCGTTCCTTGCTCACTGCGCACCGGCAGATGGCACTCTGTTTCCAGTACGTTGACGACCTGCATCATGCCTTTGCAGATGTCCTTATCAATAACGCCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTTACTACCCGTTGTT‑GATGGGCGAGCAGGGCGTGTTTATCTACAACg                                                                                                                                                                                                          <  2:270054/251‑1 (MQ=255)                                                gCACTCGCCATATGTGTCATCGCCATCGTTCCTTGCTCACTGCGCACCGGCAGATGGCACTCTGTTTCCAGTACGTTGACGACCTGCATCATGCCTTTGCAGATGTCCTTATCAATAACGCCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTTACTACCCGTTGTT‑GATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACgca                                                                                                                                                                                                   <  2:251585/251‑1 (MQ=255)                                                                    cGCCATCGTTCCTTGCTCACTGCGCACCGGCAGATGGCACTCTGTTTCCAGTACGTTGACGACCTGCATCATGCCTTTGCAGATGTCCTTATCAATAACGCCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTTACTACCCGTTGTT‑GATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTaa                                                                                                                                                                               <  1:16537/251‑1 (MQ=255)                                                                     gCCATCGTTCCTTGCTCACTGCGCACCGGCAGATGGCACTCTGTTTCCAGTACGTTGACGACCTGCATCATGCCTTTGCAGATGTCCTTATCAATAACGCCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTTACTACCCGTTGTT‑GATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAat                                                                                                                                                                              >  1:283245/1‑251 (MQ=255)                                                                                     cacggcgcTCCGTCAGATGGCACTCTGTTCCCAGTACGTTTACGATCTGCATCATGCCTTTGCAGATGTCCTTATCAATAACGCCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTTACTCCCCGTTGTT‑GATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCa                                                                                                                                                               <  2:2192/246‑1 (MQ=255)                                                                                       cTGCCCACCGGCAGATGGCTCTCTGTTTCCATTACGTTGACGACCTGCATCATGCCTTTTCAGATTTCCTTATCAATAACGCCTGCCTCGCAACGCAGGTTGAGTCTGGTTTCCATATCAGATGTTGTTTGTTTGATAACTCGTTTCCTGTGCCCCACTTACTACT‑TTTGTTTGATGGGCGGTCAGGTCTTTTTTATCTACGACGTCACGGAGCAGTTGGTGCTTTGTGTAatcatcttctttcgtca                                                                                                                                                               <  2:306478/248‑12 (MQ=255)                                                                                             aCCGGCAGATGGCACTCTGTTTCCAGTACGTTGACGACCTGCATCATGCCTTTGCAGATGTCCTTATCAATAACGCCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTTACTACCCGTTGTT‑GATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACgg                                                                                                                                                      >  2:198631/1‑251 (MQ=255)                                                                                                ggCAGATGGCACTCTGTTTCCAGTACGTTGACGACCTGCATCATGCCTTTGCAGATGTCCTTATCAATAACGCCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTTACTACCCGTTGTT‑GATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCtt                                                                                                                                                   >  1:145643/1‑251 (MQ=255)                                                                                                  cAGATGGCACTCTGTTTCCAGTACGTTGACGACCTGCATCATGCCTTTGCAGATGTCCTTATCAATAACGCCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTTACTACCCGTTGTT‑GATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTg                                                                                                                                                                                    <  2:218368/216‑1 (MQ=255)                                                                                                  cAGATGGCACTCTGTTTCCAGTACGTTGACGACCTGCATCATGCCTTTGCAGATGTCCTTATCAATAACGCCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTTACTACCCGTTGTT‑GATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTg                                                                                                                                                                                    >  1:218368/1‑216 (MQ=255)                                                                                                   aGATGGAACTCAGTTTCCCGTCAGTTGACGCCCTGCATCATGCCTTTGCAGTTGTCCTTATCAATAACGCCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCATTTCGTTTAATAACTCGGTTCCTGTTCCCCCCTTACTACCCGTTGTT‑GCTGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATTATCGACTCGCGTCCGGAACACGGCGTCgg                                                                                                                                                >  2:224250/1‑251 (MQ=255)                                                                                                    gATGGCACTCTGTTTCCAGTACGTTGACGACCTGCATCATGCCTTTGCAGATGTCCTTATCAATAACGCCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTTACTACCCGTTGTT‑GATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGt                                                                                                                                               <  2:265026/251‑1 (MQ=255)                                                                                                    gATGGCACTCTGTTTCCAGTACGTTGACGACCTGCATCATGCCTTTGCAGATGTCCTTATCAATAACGCCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTTACTACCCGTTGTT‑GATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGt                                                                                                                                               <  2:46371/251‑1 (MQ=255)                                                                                                    gATGGCACTCTGTTTCCAGTACGTTGACGACCTGCATCATGCCTTTGCAGATGTCCTTATCAATAACGCCTGCCGCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTTACTACCCGTTGTT‑GATGGGCGAGCAGGTCGTGTTTATCTACAACGGCACGCAGCTGTTGTTGCATTGGGTAAGCATCGACTCGCTTCAGGACCACTGCTTCGGt                                                                                                                                               <  2:194195/251‑1 (MQ=255)                                                                                                               gTTTCCAGTACGTTGACGACCTGCATCATGCCTTTGCAGATGTCCTTATCAATAACGCCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTTACTACCCGTTGTT‑GATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCgg                                                                                                                                                <  2:88649/239‑1 (MQ=255)                                                                                                               gTTTCCAGTACGTTGACGACCTGCATCATGCCTTTGCAGATGTCCTTATCAATAACGCCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTTACTACCCGTTGTT‑GATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCgg                                                                                                                                                >  1:88649/1‑239 (MQ=255)                                                                                                               gTTTCCAGTACGTTGACGACCTGCATCATGCCTTTGCAGATGTCCTTATCAATAACGCCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTTACTACCCGTTGTT‑GATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTaaaa                                                                                                                                    <  1:249557/251‑1 (MQ=255)                                                                                                               gTTTCCAGTACGTTGACGACCTGCATCATGCCTTTGCAGATGTCCTTATCAATAACGCCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTTACTACCCGTTGTT‑GATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTaa                                                                                                                                      <  2:81880/249‑1 (MQ=255)                                                                                                                  tCCAGTACGTTGACGACCTGCATCATGCCTTTGCAGATGTCCTTATCAATAACGCCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTTACTACCCGTTGTT‑GATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATc                                                                                                                                 >  1:43606/1‑251 (MQ=255)                                                                                                                              acgacCTGCATCATGCCTTTGCAGATGTCCTTATCAATAACGCCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTTACTACCCGTTGTT‑GATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCtg                                                                                                                     >  2:233367/1‑251 (MQ=255)                                                                                                                                          atGCCTTTGCAGATGTCCTTCTCAATAACGCCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTTACTACCCGTTGTT‑GATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCg                                                                                                         <  2:242628/251‑1 (MQ=255)                                                                                                                                          atGCCTTTGCAGATGTCCTTATCAATAACGCCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTTACTACCCGTTGTT‑GATGGGCGAGCAGTGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCTCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCg                                                                                                         <  2:136034/251‑1 (MQ=255)                                                                                                                                                  gCAGATTTCCTTATCAATAACGCCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTTACTACCCGTTGTT‑GATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAAt                                                                                                 <  2:103903/251‑1 (MQ=255)                                                                                                                                                    aGATGTCCTTATCAATAACGCCTGCCGCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCGTTCGTTTAATAACTCGGTTCCTGTTCCCCACTTACTACCCGTTGTT‑GATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATcc                                                                                               <  1:149959/251‑1 (MQ=255)                                                                                                                                                        gTCCTTATCAATAACGCCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTTACTACCCGTTGTT‑GATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGc                                                                                           <  1:2090/251‑1 (MQ=255)                                                                                                                                                             tATCAATAACGCCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTTACTACCCGTTGTT‑GATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATgg                                                                                       <  1:233367/250‑1 (MQ=255)                                                                                                                                                                  aTAACGCCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTTACTACCCGTTGTT‑GATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGtt                                                                                 >  1:82046/1‑251 (MQ=255)                                                                                                                                                                   tAACGCCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTTACTACCCGTTGTT‑Ga                                                                                                                                                                                                                                        >  1:56723/1‑99 (MQ=255)                                                                                                                                                                   tAACGCCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCGTTCGTTTAATAACTCGGTTCCTGTTCCCCACGTACTACCCGTTGTT‑Ga                                                                                                                                                                                                                                        <  2:56723/99‑1 (MQ=255)                                                                                                                                                                    aaCGCCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTTACTACCCGTTGTT‑GATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCg                                                                               <  2:99265/251‑1 (MQ=255)                                                                                                                                                                       gcctgcctCGCAACGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTTACTACCCGTTGTT‑GATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGttt                                                                            >  1:105271/1‑251 (MQ=255)                                                                                                                                                                        cctgcctCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTTACTACCCGTTGTT‑GATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTTTAATCCCAGCATGGTCAGTTCGtttt                                                                           <  2:57672/251‑1 (MQ=255)                                                                                                                                                                               cGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTTACTACCAGTTGTT‑GATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGAAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGccac                                                                    >  1:130926/1‑251 (MQ=255)                                                                                                                                                                                     gCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCATGTTCCCCACTTACTACCCGTTGTT‑GATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGtt                                                              <  1:93250/251‑1 (MQ=255)                                                                                                                                                                                        gggtGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTTACTACCCGTTGTT‑GATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGGTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTTTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATTGTCAGTTCGTTCTCCGCCTCCACGTTCCa                                                           <  2:151102/248‑1 (MQ=255)                                                                                                                                                                                            gaggcTGGTTTCCATATCAGATGCCTTGCGTTTAATAACTCGGTTCCTGTTCCCCTCTTACGACCCGTTGTT‑GATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCATCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCGGTAAAAATCGCGGATCGTCTTTGAGCGTCATCGCCTCTAATCCCAGCAGGGTCAGTTCGTTTTCCTCCTCCACGTTCCAGATc                                                       <  2:220296/247‑1 (MQ=255)                                                                                                                                                                                            gAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTTACTTCCCGTTGTT‑GATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGAt                                                        <  1:198631/250‑1 (MQ=255)                                                                                                                                                                                                  ggTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTTACTACCCGTTGTT‑GATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCAcc                                                    <  2:131449/248‑1 (MQ=255)                                                                                                                                                                                                  ggTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTTACTACCCGTTGTT‑GATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCg                                                   <  1:50002/249‑1 (MQ=255)                                                                                                                                                                                                     ttCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTTACTACCCGTTGTT‑GATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATc                                               <  2:168370/250‑1 (MQ=255)                                                                                                                                                                                                                 tGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTTACTACCCGTTGTT‑GCTGGGCGCGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTCATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATcagatcggcagcgc                                  >  1:24291/1‑238 (MQ=255)                                                                                                                                                                                                                       tCGTTTAATAACTCGGTTCCTGTTCCCCACTTACTTCCCGTTGTT‑GATTGGCGAGCAGGGCGGGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAAGCATCGGCTCGCGTCAGGACCACGGCTGCGGTGGCCTGTAAAAAGCGCGGATCGTCTGTGAGCGTCGTCGCCTCTAATCCCAGCATGGTCAGTTCGCTTTCCGCCACCACGTTCCAGATCACCGCATCTACATCGCCTCTAACAAt                             <  2:130926/250‑1 (MQ=255)                                                                                                                                                                                                                       tCGTTTAATAACTCGGTTCCTGTTCCCCACTTACTACCCGTTGTT‑GATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAAt                             <  2:67338/250‑1 (MQ=255)                                                                                                                                                                                                                                    cGGTTCCTGTTCCCCACTTACTACCCGTTGTT‑GATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAAct                 <  2:71018/249‑1 (MQ=255)                                                                                                                                                                                                                                                 ccACTTACTACCCGTTGTT‑GATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGAGCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAagagag  <  2:223645/251‑1 (MQ=255)                                                                                                                                                                                                                                                 ccACTTACTACCCGTTGTT‑GATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCATCTAATCCCAGCATGGTCCGTTCGTTTTCCGCCACCACGTTCCAGATCACCGACTCGACATAGCCTTTAACAATGCGTTGTAAACCCTCgctctaagac    >  2:53716/1‑240 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                       CGTTATCCAGCGGCTCTATTTCTTCACCACGGCGACTGCGCATCAGTGCACTCGCCATATGTGTCATCGCCATCGTTCCTTGCTCACTGCGCACCGGCAGATGGCACTCTGTTTCCAGTACGTTGACGACCTGCATCATGCCTTTGCAGATGTCCTTATCAATAACGCCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTCACTACCCGTTGTT‑GATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAG  >  NC_000913/3511221‑3511711

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑