Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,123,823	2 bp→AG	coding (231‑232/648 nt)	grxB ←	reduced glutaredoxin 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,123,823	0	G	A	100.0%	71.9 / NA	22	L78L (CTG→TTG)	grxB	reduced glutaredoxin 2
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/13);  total (9/13)
*	NC_000913	1,123,824	0	T	G	95.5%	61.9 / NA	22	L77F (TTA→TTC)	grxB	reduced glutaredoxin 2
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base G (9/12);  total (9/13)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.97e-01

ACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCCATTGACCTTGCGCAGCCACTCTTCAATGGCAGGGGAACGTTTGCCGGTCAGTAACGGTTTGCCGTCGAGTTTATCGACATAGTGAACGATGTCCATGCTTTCTGGCATATAGCGGCTGTCATCTTTTTGCAGAATGGGAACCTGTTTTTGACCGACCATCCGGGTGGGTGTTTCTGCGTCGTCGTTGAGCAGAACATGTAATTCGACGGGGATATTTTTCAGGCCGAAAATCATGCGGGCTTTGAGGCAGTAAGGGCAGTGATCGTAAATGTATAGCTTCACGTGACTCCTCCA  >  NC_000913/1123579‑1124066                                                                                                                                                                                                                                                     ||                                                                                                                                                                                                                                                   aCGACCAGTTTGTCCATCGCACGTAAACCAACTCTGATATGCGAAATCAGGCCGTCAGGGGTGGCCAGCAGGTCGGCAAAATTCCCCGCGCTGGCCTCTTTCTTGTCGACAAAAAATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGAGTTGGCAAAACGCGGCGACAGCAGTTTGTTGGCGAAGCCATTGACCTTGCGCAGCCACACTTCAATGGCAGGGGAACGTTTGCCTGTCAATAACgg                                                                                                                                                                                                                                               <  2:5189/251‑1 (MQ=255)          ttgcccAGCGCCGGTAAATCATCTCTGATATTCTTAATCAGACCGTCAGAGTGGCCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTGTTTCTTGTCGACGAAATATTTGCGCGCGGGGGGAGTAGAAAACTCATCAAATGCCGATTGGGCAAAACGCGGCAACAGCAGTTGGTTGGGGGTGCCATTGATCTTGCGCAGCTACTCGTCAATGGCAGGGGAACGTTTGCCTGGCAAGAACGGTTTGCCGt                                                                                                                                                                                                                                       <  2:168651/246‑1 (MQ=255)                                   tGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCCATTGACCTTGCGCAGCCACTCTTCAATGGCAGGGGAACGTTTGCCGGTCAAGAACGGTTTGCCGTCGAGTTTATCGACATAGTGAACGATg                                                                                                                                                                                                             >  2:101938/1‑251 (MQ=255)                                               tCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCCATTGACCTTGCGCAGCCACTCTTCAATGGCAGGGGAACGTTTGCCGGTCAAGAACGGTTTGCCGTCGAGTTTATCGACATAGTGAACGATGTCCATGCTTTCt                                                                                                                                                                                                 >  2:62871/1‑251 (MQ=255)                                                   aCCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCCATTGACCTTGCGCAGCCACTCTTCAATGGCAGGGGAACGTTTGCCGGTCAAGAACGGTTTGCCGTCGAGTTTATCGACATAGTGAACGATGTCCATGCTTTCTGGCa                                                                                                                                                                                             <  1:266800/251‑1 (MQ=255)                                                                      agGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCCATTGACCTTGCGCAGCCACTCTTCAATGGCAGGGGAACGTTTGCCGGTCAAGAACGGGTTGCCGTCGAGTTTATCGAc                                                                                                                                                                                                                          >  2:236991/1‑203 (MQ=255)                                                                      agGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCCATTGACCTTGCGCAGCCACTCTTCAAGGGCAGGGGAACGTTTGCCGGTCAAGAACGGTTTGCCGTCGAGTGTATCGAc                                                                                                                                                                                                                          <  1:236991/203‑1 (MQ=255)                                                                              aaaaTTCCCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCCATTGACCTTGCGCAGCCACTCTTCAATGGCAGGGGAACGTTTGCCGGTCAAGAACGGTTTGCCGTCGAGTTTATCGACATAGTGAACGATGTCCATGCTTTCTGGCATATAGCGGCTGTCATCTTTTTGCAGa                                                                                                                                                                   >  1:266112/1‑250 (MQ=255)                                                                                      ccGCGCTGGCCTCTTTGTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCCATTGACCTTGCGCAGCCACTCTTCAATGGCAGGGGAACGTTTGCCGGTCAAGAACGGTTGGCCTTCGAGTTTATCGACATAGTGAACGATGTCCATGCTTTCTGGCATATAGCGGCTGTCATCTTTTTGCAGAATGGGAAcc                                                                                                                                                          <  1:140234/251‑1 (MQ=255)                                                                                        gcgcTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCCATTGACCTTGCGCAGCCACTCTTCAATGGCAGGGGAACGTTTGCCGGTCAAGAACGGTTTGCCGTCGAGTTTATCGACATAGTGAACGATGTCCATGCTTTCTGGCATATAGCGGCTGTCATCTTTTTGCAGAATGGGAACCTg                                                                                                                                                        <  1:67439/251‑1 (MQ=255)                                                                                               ccTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCCATCGACATTGCGCAGCCCCTCTTCAATGGCCGGGGAACGTTTGCCGGTCAAGAACGGTTTGCCGTCGAGTTTATCGACATAGTGAACGATGTCCATGCTTTCTGGCTTATAGCGGCTGTCATCTTTTTGCAGCATGGGAACCTGTTTTTg                                                                                                                                                  >  1:311502/1‑250 (MQ=255)                                                                                                        tGTCGAAGAAATTTTTGCGCGTGGCGGGAGTAGAAAACTCATCAAATGCCTATTTGGCAAAACGCGGCCACAGCAGTTTGTGGGCGTAGCCATTGCCCTTGCGCAGCCACGCTTCAATGGCAGGGGAACGTTTGCCGGTCAAGAACGGTTTGCCGTCGAGTTTATCGACATAGTGAACGATGTCCATGCTTTCTGGCATATAGCGGCTGTCATCTTTTTGCAGAATGTGACCCTGGTTTTgactgccca                                                                                                                                          <  2:326689/249‑7 (MQ=255)                                                                                                                                gggtaGTAGAAAACGCATCAAATGCCGATTTGGCAAACCGCGGCAACAGCAGTTTGTTGGCGTAGCCATTGGCCTTGCGCAGCCACTCTTCAATGGCAGGGGAACGTTTGCCGGTCAAGAACGGTTTGCCGTCGAGTTTATCGACATAGTGAACGATGTCCATGCTTTCTGGCATATAGCGGCTGTCATCTTTTTGCAGAATGGGAACCTGTTTTTGACCGACCATCCGGGTGGGTGTTTCTGcgtcgtcg                                                                                                                <  1:126783/247‑1 (MQ=255)                                                                                                                                cggGAGTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCCATTGACCTTGCGCAGCCACTCTTCAATGGCAGGGGAACGTTTGCCGGTCAAGAACGGTTTGCCGTCGAGTTTATCGACATAGTGAACGATGTCCATGCTTTCTGGCATATAGCGGCTGTCATCTTTTTGCAGACTGGGAACCTGTTTTTGACCGACCATCCGGGTGGGTGTTTCTGcgtcgtcg                                                                                                                >  1:55427/1‑251 (MQ=255)                                                                                                                                            aCTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCCATTGACCTTGCGCAGCCACTCTTCAATGGCAGGGGAACGTTTGCCGGTCAAGAACGGTTTGCCGTCGAGTTTATCGACATAGTGAACGATGTCCATGCTTTCTGGCATATAGCGGCTGTCATCTTTTTGCAGAATGGGAACCTGTTTTTGACCGACCATCCGGGTGGGTGTTTCTGCGTCGTCGTTGAGCAGAACa                                                                                                    <  1:101938/251‑1 (MQ=255)                                                                                                                                                     aTGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCCATTGACCTTGCGCAGCCACTCTTCAATGGCAGGGGAACGTTTGCCGGTCAAGAACGGTTTGCCGTCGAGTTTATCGACATAGTGAACGATGTCCATGCTTTCTGGCATATAGCGGCTGTCATCTTTTTGCAGAATGGGAACCTGTTTTTGACCGACCATCCGGGTGGGTGTTTCTGCGTCGTCGTTGAGCAGAACATGTAATTCg                                                                                           >  1:203821/1‑251 (MQ=255)                                                                                                                                                              tGGCAAAACTCGGCAACAGCAGTTTGGTGGCGTAGCCATTGACCTTGCGCAGCCACTCTTCAATGTCAGGGGAACGTTTGCCGGTCAAGAACGGTTTGCCGTCGAGTTTATCGACATAGTGAACGATGTCCATGCTTTCTGGCATATAGCGGCTGTCATCTTTTTGCAGAATGGGAACCTGTTTTTGACCGACCATCCGGGTGGGTGTTTCTGCGTCGTCGTTGAGCAGAACATGTAATTCGACGGGGat                                                                                   <  2:203821/250‑1 (MQ=255)                                                                                                                                                                      cgcgGCAACAGCAGTTTGTTGGCGTAGCCATTGACCTTGCGCAGCCACTCTTCAATGGCAGGGGAACGTTTGCCGGTCAAGAACGGTTTGCCGTCGAGTTTATCGACATAGTGAACGATGTCCATGCTTTCTGGCATATAGCGGCTGTCATCTTTTTGCAGAATGGGAACCTGTTTTTGACCGACCATCCGGGTGGGTGTTTCTGCGTCGTCGTTGAGCAGAACATGTAATTCGACGGGGATATTTTTc                                                                            >  1:283303/1‑249 (MQ=255)                                                                                                                                                                                     ttgttgGCGTAGCCATTGACCTTGCGCAGCCACTCTTCAATGGCAGGGGAACGGTTGCCGGTCAAGAACGGTTTGCCGTCGAGTTTATCGACATAGTGAACGATGTCCATGCTTTCTGGCATATAGCGGCTGTCATCTTTTTGCAGAATGGGAACCTGTTTTTGACCGACCATCCGGGTGGGTGTTTCTGCGTCGTCGTTGAGCAGAACATGTAATTCGACGGGGATATTTTTCAGGCCGAAAATCATGc                                                            <  1:62871/250‑1 (MQ=255)                                                                                                                                                                                                                 gCCACTCTTCAATGGCAGGGGAACGTTTGCCGGTCAAGAACGGTTTGCCGTCGAGTTTATCGACATAGTGAACGATGTCCATGCTTTCTGGCATATAGCGGCTGTCATCTTTTTGCAGAATGGGAACCTGTTTTTGACCGACCATCCGGGTGGGTGTTTCTGCGTCGTCGTTGAGCAGAACATGTAATTCGACGGGGATATTTTTCAGGCCGAAAATCATGCGGGCTTTGAGGCAGTAAGGGCAGTGATCg                               <  2:63231/251‑1 (MQ=255)                                                                                                                                                                                                                           aaTGGCAGGGGAACGTTTGCCGGGCAAGAACGGTTTGCCGTCGAGTTTATCGACATAGTGAACGATGTCCATGCTTTCTGGCATATAGCGGCTGTCATCTTTTTGCAGGATGGGAAACTGGTTTTGACCGACCATCCGGGGGGGGGTTTCTGCGTCGTCGTTTAGCAGAAAATGTAATTCGACGGGGATATTTTTCAGGCCGAAAATCAAGCGGGCTTTGAGGCAGTTAGGGCAGTGATCGTAAATGtat                      >  2:248337/1‑250 (MQ=255)                                                                                                                                                                                                                                              ccGGTCAAGAACGGTTTGCCGTCGAGTTTATCGACATATTGAACGATGTCCATGCTTTCTGGCATATAGCGGCTGTCATCTTTTTGCAGAATGGGAACCTGTTTTTGACCGACCATCCGGGTGGGTGTTTCTGCGTCGTCGTTGAGCAGAACATGTAATTCGACGGGGATATTTTTCAGGCCGAAAATCATGCGGGCTTTGAGGCAGTAAGGGCAGTGATCGTAAATGTATAGCTTCACGTGACTCCTCCa  <  2:266112/251‑1 (MQ=255)                                                                                                                                                                                                                                                     ||                                                                                                                                                                                                                                                   ACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCCATTGACCTTGCGCAGCCACTCTTCAATGGCAGGGGAACGTTTGCCGGTCAGTAACGGTTTGCCGTCGAGTTTATCGACATAGTGAACGATGTCCATGCTTTCTGGCATATAGCGGCTGTCATCTTTTTGCAGAATGGGAACCTGTTTTTGACCGACCATCCGGGTGGGTGTTTCTGCGTCGTCGTTGAGCAGAACATGTAATTCGACGGGGATATTTTTCAGGCCGAAAATCATGCGGGCTTTGAGGCAGTAAGGGCAGTGATCGTAAATGTATAGCTTCACGTGACTCCTCCA  >  NC_000913/1123579‑1124066

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑