Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,003,219	T→G	T130P (ACC→CCC)	fliC ←	flagellar filament structural protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,003,219	0	T	G	100.0%	32.1 / NA	10	T130P (ACC→CCC)	fliC	flagellar filament structural protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (8/2);  total (8/2)

GCTTCCGTAGAAAGGGTAATTCCAGTAAGTTTAATATTGTTTGTGGTGGTAGCACCAAAAGCAGTTACTGGAGCACTAGTGGTAACTGTATCGTTATTTTTAACGCTAAAACCATCAAGGCCAAGAGTTTTAGCATCAATCTGCTTCAGATCGATAGTGATAGTCTGGTTATCATTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATTTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGAGGTGAAA  >  NC_000913/2002981‑2003448                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                      gctTCCGCAGACCGGGTAATTCCAGTAGGATTAATATTGTTCGTGGTGCAAACACAAAACACACTTACTCGAGCACTCGTGGTACCTGCATCGTTATTTTTCACGCTAAAACCATCAAGGCCAAGAGTTTTAGCATCAATCTGCTTCAGATCGCTAGAGATAGTCTGGTTATCATTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGGCTGACCAGATAc                                                                                                                                                                                                                           >  2:164840/1‑251 (MQ=255)                        aGTAAGTTTAATATTGTTTGTGGTGGTAGCACCAAAAGCAGTTACTGGAGCACTAGTGGTAACTGTATCGTTATTTTTAACGCTAAAACCATCAAGGCCAAGAGTTTTAGCATCAATCTGCTTCAGATCGATAGTGATAGTCTGGTTATCATTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGGCTGACCAGATACGCGGTCAATTTCATCCAGACggg                                                                                                                                                                                                    >  1:131600/1‑251 (MQ=255)                                                                                  gTAACTGTATCGTTATTTTTAACGCTAAAACCATCAAGGCCAAGAGTTTTAGCATCAATCTGCTTCAGATCGATAGTGATAGTCTGGTTATCATTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGGCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGc                                                                                                                                          >  1:271150/1‑251 (MQ=255)                                                                                                                                    aGCATCAATCTGCTTCAGATCGATAGTGATAGTCTGGTTATCATTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGGCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATTTCGGAc                                                                                         >  1:275652/1‑250 (MQ=255)                                                                                                                                     gCATCAATCTGCTTCAGATCGATAGTGATAGTCTGGTTATCATTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGGCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATTTCGGAc                                                                                         >  1:222266/1‑249 (MQ=255)                                                                                                                                       aTCAATCTGCTTCAGATCGATAGTGATAGTCTGGTTATCATTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGGCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATTTCGGACAgcg                                                                                     >  1:183289/1‑251 (MQ=255)                                                                                                                                                          atagtgatagtCTGGTTATCATTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCCCGTTCACGCCGTTGAACTGGGGCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATTTCGGACAGCGCGCCTTCGGTGGTCTgc                                                                    <  1:250852/249‑1 (MQ=255)                                                                                                                                                                         ttATCATTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGGCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATTTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACcgtc                                                   >  1:54423/1‑251 (MQ=255)                                                                                                                                                                         ttATCATTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGGCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATTTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACcgtc                                                   >  2:220300/1‑251 (MQ=255)                                                                                                                                                                                                                          cGTTCACGCCGTTGAACTGGGGCTGATCAGATTCGCGGGCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGGAGACAGATCAGACTCAGAGTGAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTTATTTCGGACAGCGCGCCTTCGGTGGGCTGCGCAACGGAGATACCGTCGTGGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGAGGTGaaa  <  1:202693/251‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                      GCTTCCGTAGAAAGGGTAATTCCAGTAAGTTTAATATTGTTTGTGGTGGTAGCACCAAAAGCAGTTACTGGAGCACTAGTGGTAACTGTATCGTTATTTTTAACGCTAAAACCATCAAGGCCAAGAGTTTTAGCATCAATCTGCTTCAGATCGATAGTGATAGTCTGGTTATCATTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATTTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGAGGTGAAA  >  NC_000913/2002981‑2003448

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑