Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,331,890	C→T	L463L (CTC→CTT)	proP →	osmolyte:H(+) symporter ProP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,331,890	0	C	T	100.0%	101.5 / NA	33	L463L (CTC→CTT)	proP	osmolyte:H(+) symporter ProP
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/25);  total (8/25)

CGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGATGCCTGCCTATTACCTGATGGTAGTGGCGGTGGTTGGTTTAATCACCGGCGTAACCATGAAAGAGACGGCAAATCGTCCGTTGAAAGGTGCGACACCGGCGGCGTCAGATATACAGGAAGCGAAGGAAATTCTCGTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACATCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCAATTGAACTCTTAAGGTTCACTTAATCTCTGACGCGCATACTCTCCTCCAGGTTAACGGAGGAGAGTGCAATGAAAAACCGTGTT  >  NC_000913/4331646‑4332130                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                 cGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGATGCCTGCCTATTACCTGATGGTAGTGGCGGTGGTTGGTTTAATCACCGGCGTAACCATGAAAGAGACGGCAAATCGTCCGTTGAAAGGTGCGACACCGGCGGCGTCAGATATACAGGAAGCGAAGGAAATTCTTGTCGAg                                                                                                                                                                                                                                            <  2:250887/251‑1 (MQ=255)         cGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGATGCCTGCCTATTACCTGATGGTAGTGGCGGTGGTTGGTTTAATCACCGGCGTAACCATGAAAGAGACGGCAAATCGGCCGTTGAAAGGTGCGACACCGGCTGCGTCAGATATGCAGGAAGCGAAGGAAATTCTTGTCGAGCATTACGa                                                                                                                                                                                                                                    <  2:21929/251‑1 (MQ=255)                   cgcTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGATGCCTGCCTATTACCTGATGGTAGTGGCGGTGGTTGGTTTAATCACCGGCGTAACCATGAAAGAGACGGCAAATCGTCCGTTGAAAGGTGCGACACCGGCGGCGTCAGATATACAGGAAGCGAAGGAAATTCTTGTCGAGCATTACGATAATATCGAg                                                                                                                                                                                                                          <  1:32303/251‑1 (MQ=255)                                aTTTAATATTGCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGATGCCTGCCTATTACCTGATGGTAGTGGCGGTGGTTGGTTTAATCACCGGCGTAACCATGAAAGAGACGGCAAATCGTCCGTTGAAAGGTGCGACACCGGCGGCGTCAGATATACAGGAAGCGAAGGAAATTCTTGTCGAGCATTACGATAAGATCTAGCAGAAAATCgatg                                                                                                                                                                                                             <  1:109255/251‑1 (MQ=255)                                       atTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGATGCCTGCCTATTACCTGATGGTAGTGGCGGTGGTTGGTTTAATCACCGGCGTAACCATGAAAGAGACGGCAAATCGTCCGTTGAAAGGTGCGACACCGGCGGCGTCAGATATACAGGAAGCGAAGGAAATTCTTGTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATATTg                                                                                                                                                                                                       <  1:219205/250‑1 (MQ=255)                                         ttCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGATGCCTGCCTATTACCTGATGGTAGTGGCGGTGGTTGGTTTAATCACCGGCGTAACCATGAAAGAGACGGCAAATCGTCCGTTGAAAGGTGCGACACCGGCGGCGTCAGATATACAGGAAGCGAAGGAAATTCTTGTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATATTGAc                                                                                                                                                                                                     <  1:89683/250‑1 (MQ=255)                                                      gCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGATGCCTGCCTATGACCTGAGGGTAGTGGCGGTGGTTGGTTTAATCACCGGCGTAACCATGAAAGAGACGGCAAATCGTCCGGTGAAATGTGCGACACCGGCGGCGTCAGATATACAGGAAGCGAAGGAAATTCTTGTCGAGCAGTACGATAATATCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCg                                                                                                                                                                                        <  1:194311/250‑1 (MQ=255)                                                                                  ggctggGCGAAAGCTCGCAGAATCTGAGGATGCCGGCCTATTCCCTGATGGTAGTGGCGGTGGTTGGTTTAATCCCCGGCGTAACCATGAAAGAGAGGGCAAATCGTCCGTTGAAAGGGGCGACACCGGCGGCGTCAGATATACAGGAAGCGAAGGAAATTCTTGTGGAGCATTACGATAATATCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTg                                                                                                                                                           <  2:81823/251‑1 (MQ=255)                                                                                              gcgcGCAGAATCTGATGAGGCCTGCCTATTACCTGATGGTAGTGGCGGTGGTTGGTTTAATCACCGGCGTAACCATGAAAGAGACGGCAACTCGTCCGTTGAAAGGTGCGACACCGGCGGCGTCAGATATACAGGAAGCGAAGGAAATTCTTGTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATATTGCCCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACATCcg                                                                                                                                               <  2:84136/248‑1 (MQ=255)                                                                                                    aGAATCTGATGATGCCTGCCTATTACCTGATGGTAGGGGCGGTGGTGGGTTTAATCACCGGCGTAACCATGAAAGAGACGGCAAATCGTCCGTTGAAAGGTGCGACACCGGCGGCGTCAGATATACAGGAAGCGAAGGAAATTCTTGTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTCCCCGCCTGGTGCAGCAACATCCGCGAAtt                                                                                                                                         <  2:318291/251‑1 (MQ=255)                                                                                                                 gccttcctATTACCTGATGTTAGTGGCGGTGGTTGGTTTAATCACCGGCGTAACCATGAAAGAGACGGAAAATCGTCCGTTGAAAGGTGCGACACCGGCGTCGTCAGATATACAGGAAGCGAAGGAAATTCTTGTCGAGCATTACGATAATATCGAGCAAAAAATCGATGATATTGACCACGAGATGGCCGTTTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACATCCGCGAATTGATGAATAAGCTg                                                                                                                            <  2:124327/251‑1 (MQ=255)                                                                                                                  cctgcctATTACCGGGTGGTAGTGGCGGTGGTTGGTTTAATCACCGGCGTAACCAGGAAAGAGACGGCAAATCGTCCGTTGAAAGGTGCGACACCGGCGGCGTCAGATATACAGGAAGCGAAGGAAATTCTTGTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACATCCGCGAATTGATGAATAAGCTGa                                                                                                                           <  2:49456/251‑1 (MQ=255)                                                                                                                     gcttATTACCGGATGGGAGTGGCGGTGGTTGGTTAAATCACCGGCGTAACCATGAAAGAGACGGCAAATCGTCCGTTAAAAGGTGCGACACCGGCGGCGTCAAATATACAGGAAGCGAAGGAAATTCTTGTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACATCCGCGAATTGATGAATAAGCTGAAAc                                                                                                                        <  2:74369/248‑1 (MQ=255)                                                                                                                        tattatcttattgTAGTTGCGGTGGTTGGTTTAATCCCCGGTGTAACCATGAAAGAGACGGCAAATCGTCCGTTGAAAGGTGCGCCTCCGGCGGCGTCAGATATACAGGAAGCGAAGGAAATTCTTTTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATATTGCCCACGAGATTGCCGATTTGCAGGCGAAACGTCCCCGCCTGGTGCAGCAACATCCGCGAATTTATGAATAAGCTGAAACgg                                                                                                                      <  1:105458/238‑1 (MQ=255)                                                                                                                                         gggggtggtTGGTTTAATCACCGGCGTAACCATGAAAGAGACGGCAAATCGTCCGTTGAAAGGTGCGACACCGGCGGCGTCAGATATACAGGAAGCGAAGTAAATTCTTGTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTTCAGGCGAAACGTACCCGCCTGGTGCAGCAACATCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCt                                                                                                    <  2:226259/248‑1 (MQ=255)                                                                                                                                           gggtggtTGGTTTAATCACCGGCGTAACCATGAAAGATCCGGCAAATCTTCCGTTGAAAGTTGCGAAACCGGCGGCGTCAGAGATACAGGAAGCGAAGGAAATTCTTGCCGACTATTACGTTAATATCGAGCAGAAAATCTATGATATGGACCACGAGATGGCCGATTTGTAGGCGAAACTTACCCGCCTGGTGCAGCAACATCCGCGAATTGATGAATAAGCTGAAACGGAGGTCCTGagggggggctgt                                                                                                  <  2:311259/250‑12 (MQ=255)                                                                                                                                           cGGTGGTGTGTTTAATCACCGGCGTAACCATGAAAGAGACGGCAAATCGTCCGTTGAAAGGTGCGACACCGGCGGCGTCAGATATACAGTAAGCGAAGGAAATTCTTGTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACATCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGt                                                                                                  <  1:25873/251‑1 (MQ=255)                                                                                                                                                       tAATCACCGGCGTAACCATGAAAGAGACGGCAAATCGTCCGTTGAAAGGTGCGACACCGGCGGCGTCAGGTATACAGGAAGCGAAGGAAATTCTTGTCGAGCATTACGATAATATCGAGCCGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGGGCAGCAACATCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGccc                                                                                      >  2:106882/1‑250 (MQ=255)                                                                                                                                                                               ggccggaaaaTCGTCCGTTGAAAGGTGCGACCCCGGCGGCGTCAGATATACAGGAAGCGAAGGAAATTCTTGTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACATCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCAATTGAACTCTTAAGGTTCACTTaa                                                              <  2:122514/244‑1 (MQ=255)                                                                                                                                                                               agaCGGCAAATCGTCCGTTGAAAGGTGCGACACCGGCGGGGTCAGATATACAGGAAGCGAAGGAAATTCTTGTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACATCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCAATTGAACTCTTAAGGTTCACTTaa                                                              <  2:165402/251‑1 (MQ=255)                                                                                                                                                                                  cGGCAAATCGTCCGTTGAAAGGTGCGACACCGGCGGCGTCAGATATACAGGAAGCGAAGGAAATTCTTGTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACATCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCAATTGAACTCTTAAGGTTCACTTAAtct                                                           >  1:163294/1‑251 (MQ=255)                                                                                                                                                                                     cAAATCGTCCGTTGAAAGGTGCGACACCGGCGGCGTCAGATATACAGGAAGCGAAGGAAATTCTTGTCGAGCATTACGATAATATCGAGCAGAAAACCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGGACCCGCCTGGTGCAGCAACATCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCAATTGAACTCTTAAGGTTCACTTAATCTCTg                                                        <  2:43896/251‑1 (MQ=255)                                                                                                                                                                                        aTCGTCCGTTGAAAGGTGCGACACCGGCGGCGTCAGATATACAGGAAGCGAAGGAAATTCTTGTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACATCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCAATTGAACTCTTAAGGTTCACTTAATCTCTGAcg                                                     >  2:92952/1‑251 (MQ=255)                                                                                                                                                                                           gacggtggaagggTGCGCCACCGACGGCGCCGAATATACAGAATGCGAAGGAAATTCTTGTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGAACCCGCCTGGTGCAGCAACATCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCAAGTGAACTCTTAAGGTTCACTTAATCTCTGACgcgc                                                  <  2:132481/240‑1 (MQ=255)                                                                                                                                                                                                 tGAAAGGTGCGACACCGGCGGCGTCAGATATACAGGAAGCGAAGGAAATTCTTGTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACATCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCAATTGAACTCTTAAGGTTCACTTAATCTCTGACGCGCATAct                                             <  1:163229/250‑1 (MQ=255)                                                                                                                                                                                                             cacCGGCGGCGTCAGATATACAGGAAGCGAAGGAAATTCTTGTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACATCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCAATTGAACTCTTAAGGTTCACTTAATCTCTGACGCGCATACTCTCCTCCAGGTTa                                <  1:92952/251‑1 (MQ=255)                                                                                                                                                                                                                  gcggcgTCAGATATACAGGAAGCGAAGGAAATTCTTGTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACATCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCAATTGAACTCTTAAGGTTCACTTAATCTCTGACGCGCATACTCTCCTCCATGTTAACgga                           <  1:239958/251‑1 (MQ=255)                                                                                                                                                                                                                    ggcgTCAGATATACAGGAAGCGAAGGAAATTCTTGTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATATTGCCCACGAGATTGCCGATTTGCAGGCGAAACTTACCCGCCTGGTGCAGCAACATCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCAATTGAACTCTTAAGGTTCACTTAATCTCTGACGCGCATACTCTCCTCCAGGTTAACggagg                         <  1:39193/251‑1 (MQ=255)                                                                                                                                                                                                                         cAGATATACAGGAAGCGAAGGAAATTCTTGTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATATTGCCCAAGAGATTGCCGATTTGCAGGCGAAACGTACCCGcc                                                                                                                                                                >  1:188168/1‑111 (MQ=255)                                                                                                                                                                                                                                  aGGAAGCGAAGGAAATTCTTGTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACATCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCAATTGAACTCTTAAGGTTCACTTAATCTCTGACGCGCATACTCTCCTCCAGGTTAACGGAGGAGAGTGCAATGaa            >  1:234851/1‑250 (MQ=255)                                                                                                                                                                                                                                      aGCGAAGGAAATTCTTGTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACATCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCAATTGAACTCTTACGGTTCACTTAATATCTGACGCGCATACTCTCCTCCAGGTTAACGGAGGAGAGTGCAATGACAAAcc       >  1:112684/1‑251 (MQ=255)                                                                                                                                                                                                                                      aGCGAAGGAAATTCTTGTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACATCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCAATTGAACTCTTAAGGTTCACTTAATCTCTGACGCGCATACTCTCCTCCAGGCTAACGGAGGAGAGTGCAATGAAAAAcc       >  2:178161/1‑251 (MQ=255)                                                                                                                                                                                                                                           aGGAAATTCTTGTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACATCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCAATTGAACTCTTAAGGTTCACTTAATCTCTGACGCGCATACTCTCCTCCAGGTTAACGGAGGAGAGTGCAATGAAAAACCGTGtt  >  1:61653/1‑251 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                 CGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGATGCCTGCCTATTACCTGATGGTAGTGGCGGTGGTTGGTTTAATCACCGGCGTAACCATGAAAGAGACGGCAAATCGTCCGTTGAAAGGTGCGACACCGGCGGCGTCAGATATACAGGAAGCGAAGGAAATTCTCGTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATATTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACATCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCAATTGAACTCTTAAGGTTCACTTAATCTCTGACGCGCATACTCTCCTCCAGGTTAACGGAGGAGAGTGCAATGAAAAACCGTGTT  >  NC_000913/4331646‑4332130

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑