Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,169,836	A→G	L180P (CTG→CCG)	ldtC ←	L,D‑transpeptidase LdtC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,169,836	0	A	G	100.0%	114.8 / NA	32	L180P (CTG→CCG)	ldtC	L,D‑transpeptidase LdtC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/16);  total (16/16)

GACGCGCACCGTTTGGTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCA‑TTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACGGTTACCGAATTTTTACCCGGCGGGTAGTAATAGAGACGCAGCTCCGCAATGTTGATCACAATGCCTTCGCGCGGCG  >  NC_000913/1169609‑1170079                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                    aaCGCGCACCGTTTGGTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTGTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCA‑TTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGc                                                                                                                                                                                                                              <  2:159020/250‑1 (MQ=255)   cgcgCACCGTTTGGTTCGGCCGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCA‑TTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCa                                                                                                                                                                                                                             >  2:71908/1‑250 (MQ=255)      gcACCGTTTTGTGCGGCAGAGACGTTTATCGGAGTGTTGATGATATTCGCTTGGGTGCCTGGGGTGACCTGGCAATAGAGTGTTTTGATTTCGTCATCCCGCAGACGAATACAGCCAGACCTTACCCGCATGCCAATGCCGAATTCGGCGTTCGTACCAGAAACCAAATAAACTCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCA‑TTGGGTTATCCGGTCCAGCCTGCACTACCGCAGGCAACTc                                                                                                                                                                                                                         <  1:49991/251‑1 (MQ=255)      gcACCGTTTGGTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGACAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCAAGACGAATCGCATGATGGCCCA‑TTGGGTTATCCGGTACAGCCGGCACTACCGCAGGCAACTc                                                                                                                                                                                                                         >  1:11229/1‑251 (MQ=255)       cACCGTTTGGTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCA‑TTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCa                                                                                                                                                                                                                        <  1:3870/251‑1 (MQ=255)                     gCAGAGACTTTTATCGGAGTGTTGATGATATTCACTGTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCATACGAATACAGCCAGAACTTCCCCGCATGCCAATGCCGAAATCGGCGTTCGTCCCATGAAGCAAATAATCGCCGCCATAGGCCGCCAGACGAAGCGCATGAGGGCCCA‑TTGGGTTAGCCGGTCCAGCCGGCACTACCGCAGGCAATTCAATTCCCTGTGCttt                                                                                                                                                                                                          <  2:152402/251‑1 (MQ=255)                            cTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCA‑TTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACgg                                                                                                                                                                                                   >  1:113937/1‑251 (MQ=255)                                tATCGGAGTGTTGATGATATTCACTTTGGTGCCGGGGGTGACCGGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCA‑TTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGgcg                                                                                                                                                                                                <  2:127488/250‑1 (MQ=255)                                               gatATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCA‑TTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTg                                                                                                                                                                                >  1:126630/1‑251 (MQ=255)                                                            gTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCCTGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCCGACGAATCGCATGATGGCCCA‑TTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCCGGTTgg                                                                                                                                                                   >  1:48274/1‑251 (MQ=255)                                                                             gttAAAGAGTGTTTGGATATCTTCATCCCGCTGACGAATACAGCCAGAACTTCCCCGCATGCCAATGCAGAAAGCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAAGCGCATGATGGCCCA‑TTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTGTATAACGGGCGCGGATGTTTTGCGGTGGCGTCCTGGTTGGGTTTGCAAGGTTGTCTg                                                                                                                                                  <  2:16843/249‑1 (MQ=255)                                                                                aaaGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCAAGATGGCCCA‑TTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCCACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTg                                                                                                                                                  >  2:792/1‑248 (MQ=255)                                                                                      tgtTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCA‑TTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACggtgg                                                                                                                                         >  2:88187/1‑251 (MQ=255)                                                                                             atatCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAAGCGCATGATGGCACA‑TTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTAACCAt                                                                                                                                  >  2:325594/1‑251 (MQ=255)                                                                                                           cAGACGAATACAGCCAGAAATTACACGCCTGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCA‑TTGGGTTATCCGGTCCAGCCGGCACTACCGAAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGACGGTGTCAGCg                                                                                                                    >  2:200932/1‑251 (MQ=255)                                                                                                                      aGCCAGAACTTACCCGCATGCCAAAGCCGAAATCGGCGTTCGTACCAGAAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCA‑TTGGGTTAGCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCt                                                                                                         <  2:296390/251‑1 (MQ=255)                                                                                                                            aaCTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCA‑TTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGa                                                                                                   <  1:88187/251‑1 (MQ=255)                                                                                                                                     gCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCA‑TTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCt                                                                                          >  1:266376/1‑251 (MQ=255)                                                                                                                                         gCCAATGAAGAAATCGGCGTTCGTACCATGAACCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCA‑TTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTg                                                                                      >  1:275532/1‑251 (MQ=255)                                                                                                                                                   aaaccGGCGTTCGTCCCAGGAAGCAAAAAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCA‑TTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAAGTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCAGTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACGGt                                                                            <  2:144078/247‑1 (MQ=255)                                                                                                                                                                           aaaTAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCA‑TTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACGGTTACCGAATTTTTACCCGGCGGgta                                                    <  1:71908/251‑1 (MQ=255)                                                                                                                                                                             aTAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCA‑TTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACGGTTACCGAATTTTTACCCGGCGGgtagt                                                  <  1:76941/251‑1 (MQ=255)                                                                                                                                                                                       ccATAGGCCGCCAGACGAATCGCATGATGGCCCA‑TTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACGGTTACCGAATTTTTACCCGGCGGGTAGTAATAGAGACg                                        >  2:226659/1‑251 (MQ=255)                                                                                                                                                                                              ccgccAGACGAATCGCATGATGGCCCA‑TTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACGGTTACCGAATTTTTAACCGGCGGGTAGAAATAGAGACGCAGCTcc                                 >  1:244368/1‑251 (MQ=255)                                                                                                                                                                                                 ccAGACGAATCGCATGATGGCCCA‑TTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACGGTTACCGAATTTTTACCCGGCGGGTAGTAATAGAGACGCAGCTCCGc                               >  1:80790/1‑250 (MQ=255)                                                                                                                                                                                                  cAGACGAATCGCATGATGGCCCA‑TTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACGGTTACCGAATTTTTACCCGGCGGGTAGTAATAGAGACGCAGCTCCGCaa                             <  1:153330/251‑1 (MQ=255)                                                                                                                                                                                                         aTCGCATGATGGCCCACGTGGGTT‑TCCGGGTCAGCCGTCACTACCGCAGGCAACGCAATTCCCTGTGCTTTATAACGGGCGCGGATGGTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGGTTGTCCGAAACGGTTGTCTCCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACGGTTACCGAATTTTTGCCCGGCGGGTAGTAATAGAGACGCAGCCCCGCTATGTTGAt                      <  2:297802/251‑1 (MQ=255)                                                                                                                                                                                                           cGCATGATGGCCCA‑GTGGGTTATCCGGCCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTGGCCGTTGGCGTCCAGGTTGGGTTTGCACGGTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCTCCTAACTGACCAAGACCTATTGGATACTCGGTTACCGAATTTTTACCCGGCGGGTAGTAATAGAGACGCAGTTCCGCAATGTGGATca                    <  2:232945/251‑1 (MQ=255)                                                                                                                                                                                                            gCATGATGGCCCA‑TTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACGGTTACCGAATTTTTACCCGGCGGGTAGTAATAGAGACGCAGCTCCGCAATGTTGATcac                   >  2:31258/1‑251 (MQ=255)                                                                                                                                                                                                                 atgGCCCA‑TTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACGGTTACCGAATTTTTACCCGGCGGGTAGTAATAGAGACGCAGCtagctcggaagagcgtcgggtt              >  2:65381/1‑230 (MQ=255)                                                                                                                                                                                                                 atgGCCCA‑TTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACGGTTACCGAATTTTTACCCGGCGGGTAGTAATAGAGACGCAGCt                                   <  1:65381/230‑1 (MQ=255)                                                                                                                                                                                                                              gTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACGGTTACCGAATTTTTACCCGGCGGGTAGTAATAGAGACGCAGCTCCGCAATGTTGATCACAATGCCTTCGCgcggcg  <  2:126630/251‑1 (MQ=255)                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                    GACGCGCACCGTTTGGTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCA‑TTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACGGTTACCGAATTTTTACCCGGCGGGTAGTAATAGAGACGCAGCTCCGCAATGTTGATCACAATGCCTTCGCGCGGCG  >  NC_000913/1169609‑1170079

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑