Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,475,493	T→C	F19F (TTT→TTC)	yjgL →	protein YjgL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,475,493	0	T	C	100.0%	72.8 / NA	22	F19F (TTT→TTC)	yjgL	protein YjgL
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/14);  total (8/14)

AACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCAAGGTTTACGGCGAGTTTGTGAAAAGAGCGTTTTTTGATATTTTTTTGTGAGTAAAATTTGTAATGCTTAGACGTTCTTATTCACTCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATGGCTCATATTAAGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAA  >  NC_000913/4475247‑4475738                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      aaCCGCTGTGCGCAGTGGGCGCGCCAGCTCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCAAGGTTTACGGCGAGTTTGTGAAAAGAGCGTTTTTTGATATTTTTTTGTGAGTAAAATTTGTAATGCTTAGACGTTCTTATTCACTCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTCAAAc                                                                                                                                                                                                                                                   <  1:129296/251‑1 (MQ=255)      gtgggcgcAGTTGGCGCGCCAGTCCAGGTTCGCAAAGCAGTAGGGAAGAGGCGGATGGCGTGATGACTTTTCGCCGTAAATAACTCAAGTTTTACGGCGAGTTTGTGAAAAGAGCGTTTTTTGATATTTTTTTGTGAGTAAAATTAGTAATGCTTAGACGTTCTTATTCACTCAAGGTGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCCACACATTACATTAGCCGACAATTTCAAACaacaa                                                                                                                                                                                                                                              <  2:250288/247‑4 (MQ=255)                       gcCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCAAGGTTTACGGCGAGTTTGTGAAAAGAGCGTTTTTTGATATTTTTTTGTGAGTAAAATTTGTAATGCTTAGACGTTCTTATTCACTCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTCAAACAAAAAAGTGAAGTTTTAAATAc                                                                                                                                                                                                                             <  1:32846/251‑1 (MQ=255)                         cAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGGAAATAACTCAAGGTTTACGGCGAGTTTGTGAAAAGAGCGTTTTTTGATATTTTTTTGTGAGTAAAATTTGTAATGCTTAGACGTTCTTATTCACTCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCTGACAATTTCAAACAAAAAAGTGAAGTTTTAAATTCCt                                                                                                                                                                                                                           <  2:316553/251‑1 (MQ=255)                                      cAAAGCAGTAGTGAAGATGCGGATGGCGTGATGACTTTTCGCCGTAAATAACTCAAGGTTTACGGCGAGTGTGTGAAAAGAGCGTTTTTTGATATTTTTTTGTGAGTAAAATTTGTAATGCTTAGACGTTCTTATTCACTCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTAGTCTCAACACATTACATTAGCCGACAATTTCAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGgaat                                                                                                                                                                                                              <  2:72408/251‑1 (MQ=255)                                                                             cGCCGTAAATAACTCAAGGTTTACGGCGAGTTTGTGAAAAGAGCGTTTTTTGATATTTTTTTGTGAGTAAAATTTGTAATGCTTAGACGTTCTTATTCACTCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTCAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGATAAc                                                                                                                                                                         >  1:250187/1‑249 (MQ=255)                                                                                                  tACGGCGAGTTTGTGAAACGAGAGTTTTTTGATATTTTTTTGTGAGTAAAATTTGTAATGCTTAGACGTTCTTATTCACTCAAGGCGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTCAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGATAACAGAAGGAatat                                                                                                                                                              >  2:299335/1‑239 (MQ=255)                                                                                                  tACGGCGAGTTTGTGAAAAGAGCGTTTTTTGATATTTTTTTGTGAGTAAAATTTGTAATGCTTAGACGTTCTTATTCACTCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTCAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGATAACAGAAGGAatat                                                                                                                                                              <  1:299335/239‑1 (MQ=255)                                                                                                   aCGGCGAGTGTGTGAAAAGAGCGGTTTTTGATATTTTTTTGTGAGTAAAATTTGTAATGCTTAGACGTTCTTATTCTCTCAAGGAGGTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAACTGCAAACAAAAAAGTGAAGTTGTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGa                                                                                                                                                 <  2:182227/251‑1 (MQ=255)                                                                                                     ggCGAGTTTGTGAAAAGAGCGTTTTTTGATATTTTTTTGGGAGTAAAATTTGTAATGCTTAGACGTTCTTATTCACTCAAGGAGTTCGTCATGAGCAAAATAACAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTCAAACAAAAAAGTGAAGTTGTAAATGCCTGGCGTGTTGGAATGAATGATTTGGCCCGTATTGCCGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGc                                                                                                                                               <  1:51700/251‑1 (MQ=255)                                                                                                      gCGAGTTTGTGAAAAGAGCGTTTTTTGATATTTTTTTGTGAGTAAAATTTGTAATGCTTAGACGTTCTTATTCACTCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTCAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCa                                                                                                                                              <  1:283436/251‑1 (MQ=255)                                                                                                                               tGATATTTTTTTGTGAGTAAAATTTGTAATGCTTAGACGTTCTTATTCACTCAAGGAGTTCGTCATGAGCAAAATATCAGATTTACATTATTCTCAACACATTACATTAGCCGACAATTTCAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGATAGCAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGACAAAGatat                                                                                                                     >  2:264485/1‑251 (MQ=255)                                                                                                                                            tgAGTAAAATTTGTAATGCTTAGACGTTCTTATTCACTCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTCAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGtt                                                                                                         >  1:284818/1‑250 (MQ=255)                                                                                                                                              aGTAAAATTTGTAATGCTTAGACGTTCTTATTCACTCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTCAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTAtg                                                                                                      >  1:150309/1‑251 (MQ=255)                                                                                                                                                         tAATGCTTAGACGTTCTTATTCACTCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTCAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGc                                                                                           >  1:255091/1‑251 (MQ=255)                                                                                                                                                                     gTTCTTATTCACTCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTCAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAAc                                                                               <  2:255091/251‑1 (MQ=255)                                                                                                                                                                                                   gCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTCAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATGGCTCATAtt                                                 <  2:158685/251‑1 (MQ=255)                                                                                                                                                                                                               aTTTAAATTATTCTCAACACATTACATTAGCCGACAATTTCAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATGGCTCATATTAAGTCCTCATCt                                     <  1:283106/251‑1 (MQ=255)                                                                                                                                                                                                                             cAACACATTACATTAGCCGACAATTTCAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATGGCTCATATTAAGTCCTCATCTTATTCTAAAGATAc                       >  1:203250/1‑251 (MQ=255)                                                                                                                                                                                                                              aaCACATTACATTAGCCGACAATTTCAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATGGCTCATATTAAGTCCTCATCTTATTCTAAAGATACt                      >  1:156084/1‑251 (MQ=255)                                                                                                                                                                                                                                             ccGACAATTTCAAACAAAAAAGTGAAGGTTTAAAGACCTGGTGTGTTGGAATGAATTATTTTGCCCTTATTGCCGGGTGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCCGGTAGAAATATGATGGCTCATATTAAGTCCTCATCTTATTCTTAAGATACTAATGGCAATGaaaaa       <  2:74601/251‑1 (MQ=255)                                                                                                                                                                                                                                                  aaTTTCAAACAAAAAAGTGAAGTGTTAAATATCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCATGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTAGGTTATGTGGAGTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATGGCACATATTAAGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAAttca  <  2:2316/251‑4 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      AACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCAAGGTTTACGGCGAGTTTGTGAAAAGAGCGTTTTTTGATATTTTTTTGTGAGTAAAATTTGTAATGCTTAGACGTTCTTATTCACTCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATGGCTCATATTAAGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAA  >  NC_000913/4475247‑4475738

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑