Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,104,024	G→T	I162I (ATC→ATA)	wbbK ←	putative glycosyltransferase WbbK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,104,024	0	G	T	100.0%	16.7 / NA	6	I162I (ATC→ATA)	wbbK	putative glycosyltransferase WbbK
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (2/4);  total (2/4)

TTGCATACGCATTTTCTGTACCACTGATAGTAAGCAGAAATTTAATATTGGATTGTTCTTTCAATTTCCTTGCTGCACTAATAATAAGCTCGTAATTTTTAAATACTCGTGGAACAGCAGGGTAAAATATTGTCAACTCAGAAGGGTTATTCTTAAATTGAGAATCATCATCAGTAAGTTGGCTTTTATCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGACAAATCCAATGCGTAGCATTCAGCTCTTTTG  >  NC_000913/2103780‑2104268                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     ttGCATTCGCATTTTCTGTACCACTGATAGTAAGCAGAAATTTAATATTGTATTGTTCTTTCAATTTCCTTGCTGCACTAATAATAAGCTCGTAATTTTTAAATACTCGTGGAACAGCAGGGTAAAATATTGTCAACTCAGAAGGGTTATTCTTAAATTGAGAATCATCATCAGTAAGTTGGCTTTTATCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTTATaaa                                                                                                                                                                                                                                                 <  2:242331/250‑1 (MQ=255)             tttCTGTACCACTGATAGTAAGCAGAAATTTAATATTGGATTGTTCTTTCAATTTCCTTGCTGCACTAATAAGAAGCTCGTAATTTTTAAATACTCGTGGAACAGCAGGGTAAAATATTGTCAACTCAGAAGGGTTATTCTTAAATTGAGAATCATCATCAGTAAGTTGGCTTTTAGCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTTATAAATTTTTCTTTCAt                                                                                                                                                                                                                                     <  1:22223/250‑1 (MQ=255)                                                                                              aaTTTTTAAATACTCGTGGAACAGCAGGGTAAAATATTGTCAACTCAGAAGGGTTATTCTTAAATTGAGAATCATCATCAGTAAGTTGGCTTTTATCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTTATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGaa                                                                                                                                                    >  2:168010/1‑250 (MQ=255)                                                                                                                                             gAAGGGTTATTCTTAAATTGAGAATCATCATCAGTAAGTTGGCTTTTATCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTTATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATaaaa                                                                                                    <  2:232110/251‑1 (MQ=255)                                                                                                                                                gggTTATTCTTAAATTGAGAATCATCATCAGTAAGTTGGCTTTTATCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTTATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAgg                                                                                                 <  1:248041/251‑1 (MQ=255)                                                                                                                                                                                                                                                ttCTTTATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGACAAATCCAATGCGTAGCATTCAGCTCTTTTg  >  1:103432/1‑250 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     TTGCATACGCATTTTCTGTACCACTGATAGTAAGCAGAAATTTAATATTGGATTGTTCTTTCAATTTCCTTGCTGCACTAATAATAAGCTCGTAATTTTTAAATACTCGTGGAACAGCAGGGTAAAATATTGTCAACTCAGAAGGGTTATTCTTAAATTGAGAATCATCATCAGTAAGTTGGCTTTTATCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGACAAATCCAATGCGTAGCATTCAGCTCTTTTG  >  NC_000913/2103780‑2104268

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑