Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	822,527	C→T	E405K (GAA→AAA)	clsB ←	cardiolipin synthase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	822,527	0	C	T	88.9%	44.8 / NA	18	E405K (GAA→AAA)	clsB	cardiolipin synthase B
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (8/8);  minor base G (0/2);  total (8/10)
Fisher's exact test for biased strand distribution p-value = 4.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

GGGCGAGCAGGTCATAGCAGCCGTAAATCAGATAGCTGACGACCACCAGCCCGACCGCACTAAGCAGCGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTCTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGATTATCGCGCAGCGTCTGGTTAAAATGAC  >  NC_000913/822282‑822763                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                             gggCGAGCAGGTCATAGCAGCCGTAAATCAGATAGCTGACGACCACCAGCCCGACCGCACTAAGCAGCGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTAccc                                                                                                                                                                                                                                         <  1:174869/251‑1 (MQ=255)                  cagcCGTAAATCAGATAGCTGACGACCACCAGCCCGACCGCACTAAGCAGCGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAGCACAAGCACCGCGATAAAAAACAGCCAGGTGAGGATCGTCTTTGCTAAGCGCCAGCGCGTGTGTGATTTACTCATCAAGGTTTTACCCCCGGGTTTTCAGTTTGTAGCCGATCCTGCTGTTCCAtt                                                                                                                                                                                                                        <  2:31729/251‑1 (MQ=255)                           aTCAGATAGCTGACGGCCACCAGCCCGACCGCACTAAGCATCGCAACTCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCTCTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAATCAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGAGCCTGCGTTTCCATTGTCGGTtgt                                                                                                                                                                                                               <  2:31495/251‑1 (MQ=255)                            tCAGATAGCTGACGACCACCAGCCCGACCGCACTAAGCAGCGCAACGAGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCCTTGTCGGTtgtg                                                                                                                                                                                                              >  1:33170/1‑251 (MQ=255)                                       acgacCACCAGCCCGACCGCACTAAGCAGCGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTGTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTTCGGGCGGATCa                                                                                                                                                                                                   <  2:166275/251‑1 (MQ=255)                                        cgacCACCAGCCCGACCGCACTAAGCAGCGTAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGGCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTTCTAAGCGCCAGCGCGGGTGTGATTTACTCAGCAGGGTGTTACCCCCGTGGTGTCAGTTGGTACCCGATCCTGCGTTTCCATTGGCTGGTGTGCGGGCTGATCaa                                                                                                                                                                                                  <  2:307111/251‑1 (MQ=255)                                                                       aaCGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGa                                                                                                                                                                     >  2:183240/1‑249 (MQ=255)                                                                                            gACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCACCACAATCACCGCGATAAAAAACAGCCAGGTGAGTATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGaa                                                                                                                                              <  2:37325/251‑1 (MQ=255)                                                                                                      aCCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTaa                                                                                                                                     >  1:312292/1‑250 (MQ=255)                                                                                                                                                          cgATAAAAAACTGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTTTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGgca                                                                                <  2:277951/251‑1 (MQ=255)                                                                                                                                                                     aGCCAGGTGAGGATCTTCTTTGCTAAGCGCCCGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGGGGAACGCCAGCAAGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGTTTc                                                                      >  1:108071/1‑250 (MQ=255)                                                                                                                                                                                          gCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGTTTCATCCACCTGCTGACAATCTGCg                                                >  1:237803/1‑251 (MQ=255)                                                                                                                                                                                                  ccAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGTTCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGTAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTGTGGGCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCaataat                                        <  2:122438/251‑1 (MQ=255)                                                                                                                                                                                                               gATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGATTa                           <  1:183240/251‑1 (MQ=255)                                                                                                                                                                                                                    aCTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCATGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCCGACGGGGCGTGTGTGCCGGAAGCCCGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCCCGCTTTTGGTCAGGGTCCACCAGGTGCGTTTGGGCGGCATGGTTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCCGATTATcgc                       >  1:185731/1‑250 (MQ=255)                                                                                                                                                                                                                            gggTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGATTATCGCGCAGCGTCt              <  2:237803/251‑1 (MQ=255)                                                                                                                                                                                                                                 ttACCCCAGTGTTTTCAGTTTTTACCCCATCCTGCGTTTCCATTGCCGGTTGTGCGCGCCCCTCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCCGCCAACCAGCGCCGGGAAGTGGCGTCAAAAGTGGAACGCCCGCACGCTTTTGGTCAAGTTCCACCCGGTGCGTTTGGGCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGATTAACGCGCAGCGTCTGGtt          >  2:164278/1‑250 (MQ=255)                                                                                                                                                                                                                                        cGTGTTTTCAGTTTTTACCCGTTCCTACGTTGCCATTGTCGGTTGTGCGGGCGGTTCCACCTGCTCCAGACGTGGCGTGTGTGCCGGCAGCCAGCCAACCCGCGCCGGGTAGTGGCGTAAAAAGTGGGACGCCAGCCCGCTTTTGGTCAGGTTCCACCCGGTGCGTTTGGGCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCAATCATGCCGTTCCGCTTATCTCGCAGCTTCTGGTTAAAATgcc  >  2:52233/1‑249 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                             GGGCGAGCAGGTCATAGCAGCCGTAAATCAGATAGCTGACGACCACCAGCCCGACCGCACTAAGCAGCGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTCTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGATTATCGCGCAGCGTCTGGTTAAAATGAC  >  NC_000913/822282‑822763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑